This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.52 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 51,043 (1.8%) Reads that were too short: 17,727 (0.6%) Reads written (passing filters): 33,316 (1.2%) Total basepairs processed: 853,219,115 bp Total written (filtered): 7,732,494 bp (0.9%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 51043 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1250 692.0 0 1250 7 7264 692.0 0 7264 8 123 692.0 0 123 9 7352 692.0 0 7352 10 46 692.0 0 46 11 55 692.0 0 55 12 22 692.0 0 22 13 43 692.0 0 43 14 20 692.0 0 20 15 36 692.0 0 36 16 40 692.0 0 40 17 50 692.0 0 50 18 23 692.0 0 23 19 38 692.0 0 38 20 34 692.0 0 34 21 25 692.0 0 25 22 40 692.0 0 40 23 39 692.0 0 39 24 32 692.0 0 32 25 39 692.0 0 39 26 35 692.0 0 35 27 38 692.0 0 38 28 34 692.0 0 34 29 49 692.0 0 49 30 43 692.0 0 43 31 48 692.0 0 48 32 38 692.0 0 38 33 29 692.0 0 29 34 21 692.0 0 21 35 38 692.0 0 38 36 46 692.0 0 46 37 35 692.0 0 35 38 40 692.0 0 40 39 44 692.0 0 44 40 55 692.0 0 55 41 52 692.0 0 52 42 49 692.0 0 49 43 32 692.0 0 32 44 19 692.0 0 19 45 30 692.0 0 30 46 43 692.0 0 43 47 35 692.0 0 35 48 29 692.0 0 29 49 34 692.0 0 34 50 31 692.0 0 31 51 31 692.0 0 31 52 43 692.0 0 43 53 54 692.0 0 54 54 34 692.0 0 34 55 49 692.0 0 49 56 56 692.0 0 56 57 24 692.0 0 24 58 41 692.0 0 41 59 37 692.0 0 37 60 40 692.0 0 40 61 42 692.0 0 42 62 30 692.0 0 30 63 39 692.0 0 39 64 30 692.0 0 30 65 27 692.0 0 27 66 21 692.0 0 21 67 49 692.0 0 49 68 49 692.0 0 49 69 43 692.0 0 43 70 56 692.0 0 56 71 111 692.0 0 111 72 44 692.0 0 44 73 127 692.0 0 127 74 80 692.0 0 80 75 88 692.0 0 88 76 50 692.0 0 50 77 35 692.0 0 35 78 40 692.0 0 40 79 48 692.0 0 48 80 70 692.0 0 70 81 54 692.0 0 54 82 61 692.0 0 61 83 45 692.0 0 45 84 63 692.0 0 63 85 56 692.0 0 56 86 51 692.0 0 51 87 53 692.0 0 53 88 56 692.0 0 56 89 55 692.0 0 55 90 54 692.0 0 54 91 51 692.0 0 51 92 49 692.0 0 49 93 30 692.0 0 30 94 42 692.0 0 42 95 64 692.0 0 64 96 51 692.0 0 51 97 41 692.0 0 41 98 52 692.0 0 52 99 72 692.0 0 72 100 70 692.0 0 70 101 767 692.0 0 767 102 291 692.0 0 291 103 453 692.0 0 453 104 67 692.0 0 67 105 69 692.0 0 69 106 49 692.0 0 49 107 50 692.0 0 50 108 55 692.0 0 55 109 66 692.0 0 66 110 88 692.0 0 88 111 331 692.0 0 331 112 226 692.0 0 226 113 234 692.0 0 234 114 93 692.0 0 93 115 139 692.0 0 139 116 69 692.0 0 69 117 88 692.0 0 88 118 86 692.0 0 86 119 63 692.0 0 63 120 43 692.0 0 43 121 72 692.0 0 72 122 68 692.0 0 68 123 114 692.0 0 114 124 114 692.0 0 114 125 104 692.0 0 104 126 60 692.0 0 60 127 48 692.0 0 48 128 67 692.0 0 67 129 64 692.0 0 64 130 90 692.0 0 90 131 108 692.0 0 108 132 341 692.0 0 341 133 201 692.0 0 201 134 98 692.0 0 98 135 62 692.0 0 62 136 83 692.0 0 83 137 127 692.0 0 127 138 105 692.0 0 105 139 241 692.0 0 241 140 101 692.0 0 101 141 234 692.0 0 234 142 121 692.0 0 121 143 141 692.0 0 141 144 316 692.0 0 316 145 264 692.0 0 264 146 401 692.0 0 401 147 56 692.0 0 56 148 121 692.0 0 121 149 118 692.0 0 118 150 107 692.0 0 107 151 105 692.0 0 105 152 72 692.0 0 72 153 67 692.0 0 67 154 146 692.0 0 146 155 113 692.0 0 113 156 157 692.0 0 157 157 92 692.0 0 92 158 287 692.0 0 287 159 76 692.0 0 76 160 73 692.0 0 73 161 76 692.0 0 76 162 83 692.0 0 83 163 107 692.0 0 107 164 123 692.0 0 123 165 82 692.0 0 82 166 103 692.0 0 103 167 75 692.0 0 75 168 124 692.0 0 124 169 182 692.0 0 182 170 731 692.0 0 731 171 214 692.0 0 214 172 300 692.0 0 300 173 48 692.0 0 48 174 32 692.0 0 32 175 56 692.0 0 56 176 55 692.0 0 55 177 115 692.0 0 115 178 266 692.0 0 266 179 135 692.0 0 135 180 139 692.0 0 139 181 63 692.0 0 63 182 75 692.0 0 75 183 72 692.0 0 72 184 87 692.0 0 87 185 70 692.0 0 70 186 82 692.0 0 82 187 70 692.0 0 70 188 79 692.0 0 79 189 71 692.0 0 71 190 60 692.0 0 60 191 56 692.0 0 56 192 63 692.0 0 63 193 46 692.0 0 46 194 47 692.0 0 47 195 49 692.0 0 49 196 74 692.0 0 74 197 98 692.0 0 98 198 88 692.0 0 88 199 136 692.0 0 136 200 78 692.0 0 78 201 68 692.0 0 68 202 120 692.0 0 120 203 101 692.0 0 101 204 145 692.0 0 145 205 197 692.0 0 197 206 130 692.0 0 130 207 61 692.0 0 61 208 62 692.0 0 62 209 75 692.0 0 75 210 135 692.0 0 135 211 115 692.0 0 115 212 229 692.0 0 229 213 80 692.0 0 80 214 89 692.0 0 89 215 64 692.0 0 64 216 96 692.0 0 96 217 75 692.0 0 75 218 73 692.0 0 73 219 74 692.0 0 74 220 64 692.0 0 64 221 82 692.0 0 82 222 71 692.0 0 71 223 118 692.0 0 118 224 212 692.0 0 212 225 210 692.0 0 210 226 302 692.0 0 302 227 203 692.0 0 203 228 145 692.0 0 145 229 99 692.0 0 99 230 57 692.0 0 57 231 60 692.0 0 60 232 61 692.0 0 61 233 92 692.0 0 92 234 80 692.0 0 80 235 78 692.0 0 78 236 129 692.0 0 129 237 96 692.0 0 96 238 70 692.0 0 70 239 88 692.0 0 88 240 87 692.0 0 87 241 82 692.0 0 82 242 69 692.0 0 69 243 117 692.0 0 117 244 157 692.0 0 157 245 370 692.0 0 370 246 205 692.0 0 205 247 296 692.0 0 296 248 148 692.0 0 148 249 95 692.0 0 95 250 80 692.0 0 80 251 65 692.0 0 65 252 53 692.0 0 53 253 91 692.0 0 91 254 81 692.0 0 81 255 71 692.0 0 71 256 172 692.0 0 172 257 249 692.0 0 249 258 190 692.0 0 190 259 199 692.0 0 199 260 112 692.0 0 112 261 123 692.0 0 123 262 113 692.0 0 113 263 185 692.0 0 185 264 556 692.0 0 556 265 304 692.0 0 304 266 365 692.0 0 365 267 93 692.0 0 93 268 111 692.0 0 111 269 72 692.0 0 72 270 95 692.0 0 95 271 151 692.0 0 151 272 236 692.0 0 236 273 213 692.0 0 213 274 167 692.0 0 167 275 111 692.0 0 111 276 80 692.0 0 80 277 102 692.0 0 102 278 102 692.0 0 102 279 87 692.0 0 87 280 169 692.0 0 169 281 161 692.0 0 161 282 239 692.0 0 239 283 290 692.0 0 290 284 1220 692.0 0 1220 285 1458 692.0 0 1458 286 1351 692.0 0 1351 287 606 692.0 0 606 288 122 692.0 0 122 289 83 692.0 0 83 290 61 692.0 0 61 291 167 692.0 0 167 292 150 692.0 0 150 293 316 692.0 0 316 294 107 692.0 0 107 295 85 692.0 0 85 296 87 692.0 0 87 297 74 692.0 0 74 298 110 692.0 0 110 299 162 692.0 0 162 300 100 692.0 0 100 301 116 692.0 0 116 Finished in 1.50 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 33,316 Reads with adapters: 7,522 (22.6%) Reads that were too short: 45 (0.1%) Reads written (passing filters): 7,477 (22.4%) Total basepairs processed: 7,732,494 bp Total written (filtered): 2,045,182 bp (26.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7522 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 4 0.0 3 0 0 1 3 16 61 0.0 3 0 50 8 3 17 7263 0.0 3 6766 415 66 16 18 17 0.0 3 4 9 2 2 19 17 0.0 3 1 0 8 8 20 39 0.0 3 6 3 1 29 21 2 0.0 3 0 0 0 2 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 1 35 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 51 2 0.0 3 0 0 0 2 52 4 0.0 3 0 0 0 4 54 3 0.0 3 0 0 1 2 59 3 0.0 3 0 0 0 3 60 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 70 15 0.0 3 0 0 0 15 73 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 5 0.0 3 0 0 0 5 91 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 96 3 0.0 3 0 0 0 3 102 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 106 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 1 112 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 166 3 0.0 3 0 0 0 3 182 4 0.0 3 0 0 0 4 183 3 0.0 3 0 0 0 3 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 3 0.0 3 0 0 0 3 200 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 208 4 0.0 3 0 0 0 4 209 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 283 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.27 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 7,477 Reads with adapters: 184 (2.5%) Reads written (passing filters): 7,477 (100.0%) Total basepairs processed: 2,045,182 bp Total written (filtered): 2,044,620 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 184 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 31.5% C: 28.3% G: 36.4% T: 3.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 176 116.8 0 176 4 6 29.2 0 6 5 2 7.3 0 2