This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.71 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 38,577 (1.4%) Reads that were too short: 10,108 (0.4%) Reads written (passing filters): 28,469 (1.0%) Total basepairs processed: 853,219,115 bp Total written (filtered): 6,992,463 bp (0.8%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 38577 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1572 692.0 0 1572 7 8732 692.0 0 8732 8 75 692.0 0 75 9 6622 692.0 0 6622 10 26 692.0 0 26 11 41 692.0 0 41 12 8 692.0 0 8 13 20 692.0 0 20 14 20 692.0 0 20 15 18 692.0 0 18 16 22 692.0 0 22 17 23 692.0 0 23 18 6 692.0 0 6 19 21 692.0 0 21 20 27 692.0 0 27 21 24 692.0 0 24 22 18 692.0 0 18 23 29 692.0 0 29 24 33 692.0 0 33 25 23 692.0 0 23 26 17 692.0 0 17 27 28 692.0 0 28 28 23 692.0 0 23 29 22 692.0 0 22 30 17 692.0 0 17 31 23 692.0 0 23 32 27 692.0 0 27 33 20 692.0 0 20 34 21 692.0 0 21 35 22 692.0 0 22 36 13 692.0 0 13 37 11 692.0 0 11 38 21 692.0 0 21 39 18 692.0 0 18 40 31 692.0 0 31 41 22 692.0 0 22 42 15 692.0 0 15 43 50 692.0 0 50 44 18 692.0 0 18 45 17 692.0 0 17 46 15 692.0 0 15 47 18 692.0 0 18 48 22 692.0 0 22 49 24 692.0 0 24 50 109 692.0 0 109 51 23 692.0 0 23 52 25 692.0 0 25 53 38 692.0 0 38 54 25 692.0 0 25 55 33 692.0 0 33 56 25 692.0 0 25 57 21 692.0 0 21 58 31 692.0 0 31 59 31 692.0 0 31 60 29 692.0 0 29 61 18 692.0 0 18 62 16 692.0 0 16 63 15 692.0 0 15 64 25 692.0 0 25 65 29 692.0 0 29 66 21 692.0 0 21 67 33 692.0 0 33 68 36 692.0 0 36 69 39 692.0 0 39 70 32 692.0 0 32 71 130 692.0 0 130 72 26 692.0 0 26 73 80 692.0 0 80 74 68 692.0 0 68 75 67 692.0 0 67 76 12 692.0 0 12 77 16 692.0 0 16 78 14 692.0 0 14 79 31 692.0 0 31 80 50 692.0 0 50 81 35 692.0 0 35 82 33 692.0 0 33 83 34 692.0 0 34 84 36 692.0 0 36 85 32 692.0 0 32 86 48 692.0 0 48 87 33 692.0 0 33 88 34 692.0 0 34 89 24 692.0 0 24 90 42 692.0 0 42 91 46 692.0 0 46 92 30 692.0 0 30 93 34 692.0 0 34 94 20 692.0 0 20 95 28 692.0 0 28 96 35 692.0 0 35 97 29 692.0 0 29 98 27 692.0 0 27 99 34 692.0 0 34 100 54 692.0 0 54 101 449 692.0 0 449 102 157 692.0 0 157 103 218 692.0 0 218 104 51 692.0 0 51 105 57 692.0 0 57 106 42 692.0 0 42 107 31 692.0 0 31 108 36 692.0 0 36 109 25 692.0 0 25 110 31 692.0 0 31 111 167 692.0 0 167 112 122 692.0 0 122 113 92 692.0 0 92 114 62 692.0 0 62 115 91 692.0 0 91 116 45 692.0 0 45 117 60 692.0 0 60 118 49 692.0 0 49 119 27 692.0 0 27 120 34 692.0 0 34 121 40 692.0 0 40 122 36 692.0 0 36 123 87 692.0 0 87 124 90 692.0 0 90 125 74 692.0 0 74 126 45 692.0 0 45 127 36 692.0 0 36 128 28 692.0 0 28 129 36 692.0 0 36 130 79 692.0 0 79 131 82 692.0 0 82 132 357 692.0 0 357 133 184 692.0 0 184 134 102 692.0 0 102 135 31 692.0 0 31 136 55 692.0 0 55 137 78 692.0 0 78 138 74 692.0 0 74 139 130 692.0 0 130 140 55 692.0 0 55 141 143 692.0 0 143 142 91 692.0 0 91 143 113 692.0 0 113 144 236 692.0 0 236 145 221 692.0 0 221 146 347 692.0 0 347 147 42 692.0 0 42 148 77 692.0 0 77 149 115 692.0 0 115 150 91 692.0 0 91 151 72 692.0 0 72 152 36 692.0 0 36 153 37 692.0 0 37 154 100 692.0 0 100 155 81 692.0 0 81 156 97 692.0 0 97 157 47 692.0 0 47 158 252 692.0 0 252 159 51 692.0 0 51 160 49 692.0 0 49 161 40 692.0 0 40 162 50 692.0 0 50 163 50 692.0 0 50 164 54 692.0 0 54 165 41 692.0 0 41 166 76 692.0 0 76 167 36 692.0 0 36 168 64 692.0 0 64 169 106 692.0 0 106 170 534 692.0 0 534 171 162 692.0 0 162 172 280 692.0 0 280 173 33 692.0 0 33 174 15 692.0 0 15 175 38 692.0 0 38 176 40 692.0 0 40 177 65 692.0 0 65 178 105 692.0 0 105 179 75 692.0 0 75 180 76 692.0 0 76 181 36 692.0 0 36 182 32 692.0 0 32 183 46 692.0 0 46 184 48 692.0 0 48 185 53 692.0 0 53 186 39 692.0 0 39 187 46 692.0 0 46 188 46 692.0 0 46 189 61 692.0 0 61 190 40 692.0 0 40 191 26 692.0 0 26 192 24 692.0 0 24 193 29 692.0 0 29 194 35 692.0 0 35 195 55 692.0 0 55 196 55 692.0 0 55 197 56 692.0 0 56 198 69 692.0 0 69 199 90 692.0 0 90 200 49 692.0 0 49 201 40 692.0 0 40 202 42 692.0 0 42 203 61 692.0 0 61 204 70 692.0 0 70 205 98 692.0 0 98 206 67 692.0 0 67 207 39 692.0 0 39 208 39 692.0 0 39 209 66 692.0 0 66 210 102 692.0 0 102 211 83 692.0 0 83 212 226 692.0 0 226 213 67 692.0 0 67 214 48 692.0 0 48 215 61 692.0 0 61 216 53 692.0 0 53 217 50 692.0 0 50 218 66 692.0 0 66 219 43 692.0 0 43 220 42 692.0 0 42 221 35 692.0 0 35 222 50 692.0 0 50 223 75 692.0 0 75 224 104 692.0 0 104 225 123 692.0 0 123 226 218 692.0 0 218 227 113 692.0 0 113 228 99 692.0 0 99 229 104 692.0 0 104 230 29 692.0 0 29 231 29 692.0 0 29 232 43 692.0 0 43 233 53 692.0 0 53 234 55 692.0 0 55 235 47 692.0 0 47 236 60 692.0 0 60 237 74 692.0 0 74 238 64 692.0 0 64 239 63 692.0 0 63 240 43 692.0 0 43 241 46 692.0 0 46 242 45 692.0 0 45 243 60 692.0 0 60 244 112 692.0 0 112 245 163 692.0 0 163 246 125 692.0 0 125 247 184 692.0 0 184 248 85 692.0 0 85 249 78 692.0 0 78 250 46 692.0 0 46 251 36 692.0 0 36 252 51 692.0 0 51 253 59 692.0 0 59 254 53 692.0 0 53 255 69 692.0 0 69 256 81 692.0 0 81 257 105 692.0 0 105 258 108 692.0 0 108 259 93 692.0 0 93 260 73 692.0 0 73 261 87 692.0 0 87 262 64 692.0 0 64 263 109 692.0 0 109 264 268 692.0 0 268 265 156 692.0 0 156 266 178 692.0 0 178 267 54 692.0 0 54 268 59 692.0 0 59 269 36 692.0 0 36 270 62 692.0 0 62 271 91 692.0 0 91 272 132 692.0 0 132 273 123 692.0 0 123 274 83 692.0 0 83 275 75 692.0 0 75 276 60 692.0 0 60 277 51 692.0 0 51 278 78 692.0 0 78 279 75 692.0 0 75 280 74 692.0 0 74 281 84 692.0 0 84 282 128 692.0 0 128 283 153 692.0 0 153 284 719 692.0 0 719 285 606 692.0 0 606 286 564 692.0 0 564 287 248 692.0 0 248 288 59 692.0 0 59 289 60 692.0 0 60 290 50 692.0 0 50 291 100 692.0 0 100 292 88 692.0 0 88 293 189 692.0 0 189 294 76 692.0 0 76 295 80 692.0 0 80 296 58 692.0 0 58 297 76 692.0 0 76 298 108 692.0 0 108 299 100 692.0 0 100 300 92 692.0 0 92 301 79 692.0 0 79 Finished in 1.45 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 28,469 Reads with adapters: 10,163 (35.7%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 10,157 (35.7%) Total basepairs processed: 6,992,463 bp Total written (filtered): 2,778,367 bp (39.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10163 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 1 0.0 3 0 0 1 19 42 0.0 3 0 39 0 2 1 20 8686 0.0 4 8529 147 9 1 21 16 0.0 4 2 14 22 6 0.0 4 2 4 23 1382 0.0 4 1352 22 0 5 3 24 4 0.0 4 3 1 25 1 0.0 4 0 0 1 26 2 0.0 4 0 0 0 2 27 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 127 14 0.0 4 0 0 0 0 14 228 2 0.0 4 0 0 0 0 2 229 1 0.0 4 0 0 0 0 1 237 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.35 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 10,157 Reads with adapters: 394 (3.9%) Reads written (passing filters): 10,157 (100.0%) Total basepairs processed: 2,778,367 bp Total written (filtered): 2,774,172 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 394 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.1% C: 4.6% G: 88.6% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 20 158.7 0 20 6 1 2.5 0 1 9 87 0.0 0 4 83 10 138 0.0 1 4 134 11 128 0.0 1 2 126 12 14 0.0 1 0 14 20 4 0.0 1 3 1 152 1 0.0 1 0 1 158 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.