This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.45 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 266,733 (9.4%) Reads that were too short: 113,699 (4.0%) Reads written (passing filters): 153,034 (5.4%) Total basepairs processed: 853,219,115 bp Total written (filtered): 42,662,475 bp (5.0%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 266733 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1121 692.0 0 1121 7 2665 692.0 0 2665 8 66634 692.0 0 66634 9 62045 692.0 0 62045 10 76 692.0 0 76 11 45 692.0 0 45 12 30 692.0 0 30 13 11 692.0 0 11 14 18 692.0 0 18 15 18 692.0 0 18 16 11 692.0 0 11 17 14 692.0 0 14 18 7 692.0 0 7 19 9 692.0 0 9 20 19 692.0 0 19 21 13 692.0 0 13 22 24 692.0 0 24 23 9 692.0 0 9 24 21 692.0 0 21 25 18 692.0 0 18 26 39 692.0 0 39 27 13 692.0 0 13 28 21 692.0 0 21 29 10 692.0 0 10 30 13 692.0 0 13 31 18 692.0 0 18 32 18 692.0 0 18 33 19 692.0 0 19 34 31 692.0 0 31 35 10 692.0 0 10 36 32 692.0 0 32 37 13 692.0 0 13 38 32 692.0 0 32 39 13 692.0 0 13 40 13 692.0 0 13 41 26 692.0 0 26 42 30 692.0 0 30 43 62 692.0 0 62 44 10 692.0 0 10 45 20 692.0 0 20 46 15 692.0 0 15 47 16 692.0 0 16 48 28 692.0 0 28 49 38 692.0 0 38 50 31 692.0 0 31 51 23 692.0 0 23 52 22 692.0 0 22 53 11 692.0 0 11 54 18 692.0 0 18 55 23 692.0 0 23 56 68 692.0 0 68 57 99 692.0 0 99 58 94 692.0 0 94 59 26 692.0 0 26 60 25 692.0 0 25 61 57 692.0 0 57 62 25 692.0 0 25 63 28 692.0 0 28 64 53 692.0 0 53 65 32 692.0 0 32 66 14 692.0 0 14 67 30 692.0 0 30 68 30 692.0 0 30 69 165 692.0 0 165 70 294 692.0 0 294 71 325 692.0 0 325 72 58 692.0 0 58 73 37 692.0 0 37 74 55 692.0 0 55 75 94 692.0 0 94 76 165 692.0 0 165 77 68 692.0 0 68 78 83 692.0 0 83 79 94 692.0 0 94 80 57 692.0 0 57 81 60 692.0 0 60 82 87 692.0 0 87 83 69 692.0 0 69 84 63 692.0 0 63 85 208 692.0 0 208 86 95 692.0 0 95 87 79 692.0 0 79 88 113 692.0 0 113 89 1860 692.0 0 1860 90 893 692.0 0 893 91 6034 692.0 0 6034 92 54 692.0 0 54 93 30 692.0 0 30 94 60 692.0 0 60 95 25 692.0 0 25 96 34 692.0 0 34 97 136 692.0 0 136 98 66 692.0 0 66 99 103 692.0 0 103 100 59 692.0 0 59 101 34 692.0 0 34 102 38 692.0 0 38 103 28 692.0 0 28 104 21 692.0 0 21 105 23 692.0 0 23 106 18 692.0 0 18 107 25 692.0 0 25 108 40 692.0 0 40 109 29 692.0 0 29 110 31 692.0 0 31 111 62 692.0 0 62 112 76 692.0 0 76 113 71 692.0 0 71 114 19 692.0 0 19 115 15 692.0 0 15 116 21 692.0 0 21 117 64 692.0 0 64 118 29 692.0 0 29 119 22 692.0 0 22 120 39 692.0 0 39 121 28 692.0 0 28 122 16 692.0 0 16 123 25 692.0 0 25 124 20 692.0 0 20 125 28 692.0 0 28 126 26 692.0 0 26 127 14 692.0 0 14 128 56 692.0 0 56 129 34 692.0 0 34 130 41 692.0 0 41 131 24 692.0 0 24 132 36 692.0 0 36 133 30 692.0 0 30 134 44 692.0 0 44 135 27 692.0 0 27 136 26 692.0 0 26 137 74 692.0 0 74 138 26 692.0 0 26 139 48 692.0 0 48 140 32 692.0 0 32 141 28 692.0 0 28 142 26 692.0 0 26 143 24 692.0 0 24 144 27 692.0 0 27 145 42 692.0 0 42 146 39 692.0 0 39 147 37 692.0 0 37 148 181 692.0 0 181 149 97 692.0 0 97 150 242 692.0 0 242 151 24 692.0 0 24 152 27 692.0 0 27 153 37 692.0 0 37 154 40 692.0 0 40 155 31 692.0 0 31 156 121 692.0 0 121 157 76 692.0 0 76 158 34 692.0 0 34 159 67 692.0 0 67 160 61 692.0 0 61 161 32 692.0 0 32 162 40 692.0 0 40 163 31 692.0 0 31 164 29 692.0 0 29 165 33 692.0 0 33 166 32 692.0 0 32 167 51 692.0 0 51 168 389 692.0 0 389 169 152 692.0 0 152 170 138 692.0 0 138 171 31 692.0 0 31 172 31 692.0 0 31 173 30 692.0 0 30 174 76 692.0 0 76 175 109 692.0 0 109 176 1663 692.0 0 1663 177 745 692.0 0 745 178 312 692.0 0 312 179 95 692.0 0 95 180 27 692.0 0 27 181 16 692.0 0 16 182 42 692.0 0 42 183 48 692.0 0 48 184 41 692.0 0 41 185 32 692.0 0 32 186 30 692.0 0 30 187 42 692.0 0 42 188 28 692.0 0 28 189 34 692.0 0 34 190 27 692.0 0 27 191 26 692.0 0 26 192 26 692.0 0 26 193 35 692.0 0 35 194 48 692.0 0 48 195 38 692.0 0 38 196 49 692.0 0 49 197 26 692.0 0 26 198 31 692.0 0 31 199 21 692.0 0 21 200 20 692.0 0 20 201 27 692.0 0 27 202 132 692.0 0 132 203 123 692.0 0 123 204 151 692.0 0 151 205 59 692.0 0 59 206 38 692.0 0 38 207 126 692.0 0 126 208 165 692.0 0 165 209 142 692.0 0 142 210 134 692.0 0 134 211 75 692.0 0 75 212 100 692.0 0 100 213 64 692.0 0 64 214 37 692.0 0 37 215 81 692.0 0 81 216 100 692.0 0 100 217 85 692.0 0 85 218 172 692.0 0 172 219 340 692.0 0 340 220 2771 692.0 0 2771 221 1156 692.0 0 1156 222 1938 692.0 0 1938 223 98 692.0 0 98 224 45 692.0 0 45 225 244 692.0 0 244 226 37 692.0 0 37 227 67 692.0 0 67 228 39 692.0 0 39 229 26 692.0 0 26 230 93 692.0 0 93 231 83 692.0 0 83 232 303 692.0 0 303 233 67 692.0 0 67 234 77 692.0 0 77 235 84 692.0 0 84 236 45 692.0 0 45 237 53 692.0 0 53 238 48 692.0 0 48 239 167 692.0 0 167 240 127 692.0 0 127 241 271 692.0 0 271 242 137 692.0 0 137 243 147 692.0 0 147 244 117 692.0 0 117 245 103 692.0 0 103 246 84 692.0 0 84 247 50 692.0 0 50 248 54 692.0 0 54 249 105 692.0 0 105 250 84 692.0 0 84 251 113 692.0 0 113 252 66 692.0 0 66 253 115 692.0 0 115 254 63 692.0 0 63 255 132 692.0 0 132 256 223 692.0 0 223 257 163 692.0 0 163 258 79 692.0 0 79 259 109 692.0 0 109 260 156 692.0 0 156 261 152 692.0 0 152 262 397 692.0 0 397 263 260 692.0 0 260 264 746 692.0 0 746 265 300 692.0 0 300 266 263 692.0 0 263 267 198 692.0 0 198 268 350 692.0 0 350 269 1442 692.0 0 1442 270 1417 692.0 0 1417 271 2885 692.0 0 2885 272 229 692.0 0 229 273 118 692.0 0 118 274 786 692.0 0 786 275 200 692.0 0 200 276 1073 692.0 0 1073 277 197 692.0 0 197 278 299 692.0 0 299 279 430 692.0 0 430 280 713 692.0 0 713 281 1566 692.0 0 1566 282 6863 692.0 0 6863 283 39350 692.0 0 39350 284 21297 692.0 0 21297 285 17194 692.0 0 17194 286 1235 692.0 0 1235 287 46 692.0 0 46 288 135 692.0 0 135 289 206 692.0 0 206 290 587 692.0 0 587 291 169 692.0 0 169 292 43 692.0 0 43 293 44 692.0 0 44 294 48 692.0 0 48 295 29 692.0 0 29 296 49 692.0 0 49 297 86 692.0 0 86 298 103 692.0 0 103 299 32 692.0 0 32 300 49 692.0 0 49 301 50 692.0 0 50 Finished in 7.46 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 153,034 Reads with adapters: 68,233 (44.6%) Reads that were too short: 119 (0.1%) Reads written (passing filters): 68,114 (44.5%) Total basepairs processed: 42,662,475 bp Total written (filtered): 18,769,492 bp (44.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 68233 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 16 0.0 2 0 0 0 16 15 44 0.0 3 0 0 19 25 16 674 0.0 3 0 534 95 45 17 66493 0.0 3 61650 3938 736 169 18 207 0.0 3 8 120 16 63 19 232 0.0 3 0 1 130 101 20 251 0.0 3 0 0 1 250 29 1 0.0 3 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 43 20 0.0 3 0 0 1 19 45 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 80 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 82 6 0.0 3 0 0 0 6 83 2 0.0 3 0 0 0 2 84 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 1 1 101 11 0.0 3 0 0 0 11 102 105 0.0 3 0 0 0 105 103 3 0.0 3 0 0 0 3 104 1 0.0 3 0 0 0 1 105 24 0.0 3 0 0 2 22 106 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 128 3 0.0 3 0 0 0 3 131 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 142 1 0.0 3 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 153 4 0.0 3 0 0 0 4 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 178 8 0.0 3 0 0 1 7 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 11 0.0 3 0 0 1 10 198 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 11 0.0 3 0 0 0 11 204 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 206 3 0.0 3 0 0 0 3 211 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 224 2 0.0 3 0 0 0 2 233 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 280 4 0.0 3 0 0 0 4 281 13 0.0 3 0 0 0 13 282 9 0.0 3 0 0 0 9 285 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.37 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 68,114 Reads with adapters: 399 (0.6%) Reads written (passing filters): 68,114 (100.0%) Total basepairs processed: 18,769,492 bp Total written (filtered): 18,768,201 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 399 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 43.4% C: 19.3% G: 28.1% T: 9.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 345 1064.3 0 345 4 45 266.1 0 45 5 3 66.5 0 3 6 1 16.6 0 1 11 5 0.0 1 0 5