This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.56 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 241,679 (8.5%) Reads that were too short: 87,366 (3.1%) Reads written (passing filters): 154,313 (5.4%) Total basepairs processed: 853,219,115 bp Total written (filtered): 43,344,259 bp (5.1%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 241679 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1084 692.0 0 1084 7 1932 692.0 0 1932 8 64383 692.0 0 64383 9 69601 692.0 0 69601 10 63 692.0 0 63 11 26 692.0 0 26 12 11 692.0 0 11 13 10 692.0 0 10 14 7 692.0 0 7 15 7 692.0 0 7 16 11 692.0 0 11 17 2 692.0 0 2 18 12 692.0 0 12 19 5 692.0 0 5 20 6 692.0 0 6 21 135 692.0 0 135 22 9 692.0 0 9 23 6 692.0 0 6 24 5 692.0 0 5 25 6 692.0 0 6 26 8 692.0 0 8 27 9 692.0 0 9 28 10 692.0 0 10 29 6 692.0 0 6 30 1 692.0 0 1 31 11 692.0 0 11 32 2 692.0 0 2 33 5 692.0 0 5 34 9 692.0 0 9 35 15 692.0 0 15 36 15 692.0 0 15 37 6 692.0 0 6 38 21 692.0 0 21 39 3 692.0 0 3 40 10 692.0 0 10 41 7 692.0 0 7 42 22 692.0 0 22 43 82 692.0 0 82 44 1 692.0 0 1 45 18 692.0 0 18 46 13 692.0 0 13 47 11 692.0 0 11 48 14 692.0 0 14 49 29 692.0 0 29 50 16 692.0 0 16 51 21 692.0 0 21 52 21 692.0 0 21 53 6 692.0 0 6 54 11 692.0 0 11 55 13 692.0 0 13 56 883 692.0 0 883 57 167 692.0 0 167 58 71 692.0 0 71 59 15 692.0 0 15 60 10 692.0 0 10 61 36 692.0 0 36 62 18 692.0 0 18 63 17 692.0 0 17 64 26 692.0 0 26 65 17 692.0 0 17 66 12 692.0 0 12 67 12 692.0 0 12 68 17 692.0 0 17 69 117 692.0 0 117 70 175 692.0 0 175 71 155 692.0 0 155 72 53 692.0 0 53 73 21 692.0 0 21 74 44 692.0 0 44 75 48 692.0 0 48 76 128 692.0 0 128 77 58 692.0 0 58 78 55 692.0 0 55 79 73 692.0 0 73 80 38 692.0 0 38 81 41 692.0 0 41 82 53 692.0 0 53 83 51 692.0 0 51 84 64 692.0 0 64 85 228 692.0 0 228 86 59 692.0 0 59 87 55 692.0 0 55 88 64 692.0 0 64 89 1500 692.0 0 1500 90 610 692.0 0 610 91 5006 692.0 0 5006 92 40 692.0 0 40 93 14 692.0 0 14 94 13 692.0 0 13 95 15 692.0 0 15 96 13 692.0 0 13 97 48 692.0 0 48 98 35 692.0 0 35 99 37 692.0 0 37 100 42 692.0 0 42 101 14 692.0 0 14 102 16 692.0 0 16 103 15 692.0 0 15 104 17 692.0 0 17 105 14 692.0 0 14 106 15 692.0 0 15 107 20 692.0 0 20 108 23 692.0 0 23 109 18 692.0 0 18 110 24 692.0 0 24 111 22 692.0 0 22 112 55 692.0 0 55 113 29 692.0 0 29 114 7 692.0 0 7 115 12 692.0 0 12 116 5 692.0 0 5 117 36 692.0 0 36 118 12 692.0 0 12 119 11 692.0 0 11 120 16 692.0 0 16 121 12 692.0 0 12 122 17 692.0 0 17 123 14 692.0 0 14 124 9 692.0 0 9 125 14 692.0 0 14 126 18 692.0 0 18 127 16 692.0 0 16 128 54 692.0 0 54 129 7 692.0 0 7 130 20 692.0 0 20 131 8 692.0 0 8 132 18 692.0 0 18 133 19 692.0 0 19 134 18 692.0 0 18 135 14 692.0 0 14 136 19 692.0 0 19 137 68 692.0 0 68 138 16 692.0 0 16 139 41 692.0 0 41 140 29 692.0 0 29 141 24 692.0 0 24 142 17 692.0 0 17 143 11 692.0 0 11 144 14 692.0 0 14 145 20 692.0 0 20 146 28 692.0 0 28 147 24 692.0 0 24 148 57 692.0 0 57 149 46 692.0 0 46 150 94 692.0 0 94 151 15 692.0 0 15 152 21 692.0 0 21 153 23 692.0 0 23 154 19 692.0 0 19 155 22 692.0 0 22 156 96 692.0 0 96 157 60 692.0 0 60 158 28 692.0 0 28 159 40 692.0 0 40 160 42 692.0 0 42 161 31 692.0 0 31 162 26 692.0 0 26 163 24 692.0 0 24 164 25 692.0 0 25 165 24 692.0 0 24 166 23 692.0 0 23 167 41 692.0 0 41 168 337 692.0 0 337 169 136 692.0 0 136 170 119 692.0 0 119 171 22 692.0 0 22 172 26 692.0 0 26 173 31 692.0 0 31 174 71 692.0 0 71 175 134 692.0 0 134 176 2003 692.0 0 2003 177 920 692.0 0 920 178 329 692.0 0 329 179 34 692.0 0 34 180 20 692.0 0 20 181 8 692.0 0 8 182 24 692.0 0 24 183 22 692.0 0 22 184 21 692.0 0 21 185 16 692.0 0 16 186 25 692.0 0 25 187 31 692.0 0 31 188 12 692.0 0 12 189 28 692.0 0 28 190 23 692.0 0 23 191 19 692.0 0 19 192 13 692.0 0 13 193 26 692.0 0 26 194 30 692.0 0 30 195 22 692.0 0 22 196 23 692.0 0 23 197 24 692.0 0 24 198 19 692.0 0 19 199 11 692.0 0 11 200 16 692.0 0 16 201 20 692.0 0 20 202 69 692.0 0 69 203 46 692.0 0 46 204 46 692.0 0 46 205 18 692.0 0 18 206 27 692.0 0 27 207 84 692.0 0 84 208 84 692.0 0 84 209 88 692.0 0 88 210 44 692.0 0 44 211 41 692.0 0 41 212 43 692.0 0 43 213 40 692.0 0 40 214 31 692.0 0 31 215 58 692.0 0 58 216 74 692.0 0 74 217 85 692.0 0 85 218 146 692.0 0 146 219 276 692.0 0 276 220 2837 692.0 0 2837 221 1278 692.0 0 1278 222 1913 692.0 0 1913 223 81 692.0 0 81 224 35 692.0 0 35 225 247 692.0 0 247 226 33 692.0 0 33 227 63 692.0 0 63 228 16 692.0 0 16 229 13 692.0 0 13 230 59 692.0 0 59 231 64 692.0 0 64 232 139 692.0 0 139 233 52 692.0 0 52 234 94 692.0 0 94 235 40 692.0 0 40 236 31 692.0 0 31 237 19 692.0 0 19 238 31 692.0 0 31 239 78 692.0 0 78 240 75 692.0 0 75 241 138 692.0 0 138 242 86 692.0 0 86 243 84 692.0 0 84 244 62 692.0 0 62 245 58 692.0 0 58 246 61 692.0 0 61 247 52 692.0 0 52 248 34 692.0 0 34 249 59 692.0 0 59 250 65 692.0 0 65 251 92 692.0 0 92 252 38 692.0 0 38 253 74 692.0 0 74 254 54 692.0 0 54 255 80 692.0 0 80 256 167 692.0 0 167 257 112 692.0 0 112 258 51 692.0 0 51 259 113 692.0 0 113 260 118 692.0 0 118 261 117 692.0 0 117 262 193 692.0 0 193 263 108 692.0 0 108 264 230 692.0 0 230 265 159 692.0 0 159 266 206 692.0 0 206 267 107 692.0 0 107 268 207 692.0 0 207 269 669 692.0 0 669 270 651 692.0 0 651 271 1243 692.0 0 1243 272 181 692.0 0 181 273 79 692.0 0 79 274 1154 692.0 0 1154 275 240 692.0 0 240 276 1576 692.0 0 1576 277 131 692.0 0 131 278 225 692.0 0 225 279 356 692.0 0 356 280 438 692.0 0 438 281 1286 692.0 0 1286 282 5320 692.0 0 5320 283 30533 692.0 0 30533 284 17650 692.0 0 17650 285 12228 692.0 0 12228 286 934 692.0 0 934 287 41 692.0 0 41 288 93 692.0 0 93 289 130 692.0 0 130 290 288 692.0 0 288 291 147 692.0 0 147 292 32 692.0 0 32 293 41 692.0 0 41 294 44 692.0 0 44 295 31 692.0 0 31 296 37 692.0 0 37 297 46 692.0 0 46 298 45 692.0 0 45 299 35 692.0 0 35 300 27 692.0 0 27 301 12 692.0 0 12 Finished in 8.11 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 154,313 Reads with adapters: 72,057 (46.7%) Reads that were too short: 52 (0.0%) Reads written (passing filters): 72,005 (46.7%) Total basepairs processed: 43,344,259 bp Total written (filtered): 19,567,969 bp (45.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 72057 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 6 0.0 3 0 0 5 1 19 187 0.0 3 0 181 6 20 71407 0.0 4 70068 1288 47 3 1 21 156 0.0 4 30 123 3 22 8 0.0 4 0 0 8 23 48 0.0 4 1 0 0 43 4 24 5 0.0 4 0 0 0 0 5 25 3 0.0 4 1 0 0 1 1 29 1 0.0 4 1 30 1 0.0 4 0 1 33 1 0.0 4 0 1 45 1 0.0 4 0 1 93 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 165 0.0 4 0 0 0 0 165 128 9 0.0 4 0 0 0 0 9 135 3 0.0 4 0 0 0 0 3 145 1 0.0 4 0 0 0 0 1 146 7 0.0 4 0 0 0 2 5 147 3 0.0 4 0 0 0 0 3 178 1 0.0 4 0 0 0 0 1 184 1 0.0 4 1 195 1 0.0 4 1 213 1 0.0 4 0 0 0 0 1 221 2 0.0 4 0 0 0 0 2 224 1 0.0 4 0 0 0 0 1 228 10 0.0 4 0 0 0 0 10 229 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 235 4 0.0 4 0 0 0 0 4 237 12 0.0 4 0 0 0 0 12 257 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.56 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 72,005 Reads with adapters: 9,397 (13.1%) Reads written (passing filters): 72,005 (100.0%) Total basepairs processed: 19,567,969 bp Total written (filtered): 19,474,200 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 9397 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.7% C: 3.3% G: 93.3% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 16 1125.1 0 16 4 14 281.3 0 14 5 8 70.3 0 8 6 2 17.6 0 2 8 13 1.1 0 13 9 3931 0.3 0 136 3795 10 4141 0.1 1 180 3961 11 1185 0.0 1 15 1170 12 9 0.0 1 0 9 13 7 0.0 1 0 7 15 52 0.0 1 36 16 16 2 0.0 1 2 57 1 0.0 1 0 1 70 3 0.0 1 3 104 1 0.0 1 1 150 5 0.0 1 0 5 208 3 0.0 1 2 1 223 3 0.0 1 3 260 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.