This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.80 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 144,343 (5.1%) Reads that were too short: 31,519 (1.1%) Reads written (passing filters): 112,824 (4.0%) Total basepairs processed: 853,219,115 bp Total written (filtered): 26,307,829 bp (3.1%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 144343 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2649 692.0 0 2649 7 28809 692.0 0 28809 8 20141 692.0 0 20141 9 61 692.0 0 61 10 47 692.0 0 47 11 46 692.0 0 46 12 36 692.0 0 36 13 41 692.0 0 41 14 47 692.0 0 47 15 38 692.0 0 38 16 39 692.0 0 39 17 56 692.0 0 56 18 81 692.0 0 81 19 58 692.0 0 58 20 176 692.0 0 176 21 58 692.0 0 58 22 29 692.0 0 29 23 50 692.0 0 50 24 34 692.0 0 34 25 31 692.0 0 31 26 30 692.0 0 30 27 49 692.0 0 49 28 44 692.0 0 44 29 57 692.0 0 57 30 91 692.0 0 91 31 32 692.0 0 32 32 25 692.0 0 25 33 42 692.0 0 42 34 65 692.0 0 65 35 31 692.0 0 31 36 72 692.0 0 72 37 58 692.0 0 58 38 63 692.0 0 63 39 44 692.0 0 44 40 59 692.0 0 59 41 47 692.0 0 47 42 204 692.0 0 204 43 55 692.0 0 55 44 38 692.0 0 38 45 61 692.0 0 61 46 199 692.0 0 199 47 42 692.0 0 42 48 44 692.0 0 44 49 34 692.0 0 34 50 61 692.0 0 61 51 62 692.0 0 62 52 56 692.0 0 56 53 61 692.0 0 61 54 50 692.0 0 50 55 55 692.0 0 55 56 97 692.0 0 97 57 38 692.0 0 38 58 56 692.0 0 56 59 56 692.0 0 56 60 78 692.0 0 78 61 34 692.0 0 34 62 48 692.0 0 48 63 69 692.0 0 69 64 104 692.0 0 104 65 147 692.0 0 147 66 249 692.0 0 249 67 455 692.0 0 455 68 163 692.0 0 163 69 252 692.0 0 252 70 435 692.0 0 435 71 103 692.0 0 103 72 134 692.0 0 134 73 207 692.0 0 207 74 187 692.0 0 187 75 206 692.0 0 206 76 210 692.0 0 210 77 290 692.0 0 290 78 270 692.0 0 270 79 521 692.0 0 521 80 6091 692.0 0 6091 81 8401 692.0 0 8401 82 11877 692.0 0 11877 83 220 692.0 0 220 84 118 692.0 0 118 85 98 692.0 0 98 86 132 692.0 0 132 87 88 692.0 0 88 88 81 692.0 0 81 89 156 692.0 0 156 90 46 692.0 0 46 91 32 692.0 0 32 92 38 692.0 0 38 93 63 692.0 0 63 94 41 692.0 0 41 95 83 692.0 0 83 96 140 692.0 0 140 97 65 692.0 0 65 98 27 692.0 0 27 99 55 692.0 0 55 100 41 692.0 0 41 101 27 692.0 0 27 102 40 692.0 0 40 103 49 692.0 0 49 104 31 692.0 0 31 105 39 692.0 0 39 106 37 692.0 0 37 107 42 692.0 0 42 108 29 692.0 0 29 109 29 692.0 0 29 110 49 692.0 0 49 111 43 692.0 0 43 112 50 692.0 0 50 113 55 692.0 0 55 114 57 692.0 0 57 115 39 692.0 0 39 116 36 692.0 0 36 117 90 692.0 0 90 118 116 692.0 0 116 119 178 692.0 0 178 120 200 692.0 0 200 121 226 692.0 0 226 122 197 692.0 0 197 123 54 692.0 0 54 124 112 692.0 0 112 125 157 692.0 0 157 126 152 692.0 0 152 127 223 692.0 0 223 128 685 692.0 0 685 129 126 692.0 0 126 130 30 692.0 0 30 131 51 692.0 0 51 132 44 692.0 0 44 133 61 692.0 0 61 134 57 692.0 0 57 135 71 692.0 0 71 136 113 692.0 0 113 137 41 692.0 0 41 138 46 692.0 0 46 139 64 692.0 0 64 140 42 692.0 0 42 141 79 692.0 0 79 142 57 692.0 0 57 143 50 692.0 0 50 144 74 692.0 0 74 145 58 692.0 0 58 146 100 692.0 0 100 147 76 692.0 0 76 148 80 692.0 0 80 149 89 692.0 0 89 150 146 692.0 0 146 151 35 692.0 0 35 152 68 692.0 0 68 153 51 692.0 0 51 154 67 692.0 0 67 155 44 692.0 0 44 156 63 692.0 0 63 157 38 692.0 0 38 158 41 692.0 0 41 159 48 692.0 0 48 160 49 692.0 0 49 161 72 692.0 0 72 162 67 692.0 0 67 163 51 692.0 0 51 164 72 692.0 0 72 165 91 692.0 0 91 166 71 692.0 0 71 167 261 692.0 0 261 168 125 692.0 0 125 169 1015 692.0 0 1015 170 62 692.0 0 62 171 76 692.0 0 76 172 69 692.0 0 69 173 106 692.0 0 106 174 139 692.0 0 139 175 268 692.0 0 268 176 105 692.0 0 105 177 129 692.0 0 129 178 145 692.0 0 145 179 94 692.0 0 94 180 120 692.0 0 120 181 121 692.0 0 121 182 113 692.0 0 113 183 126 692.0 0 126 184 141 692.0 0 141 185 220 692.0 0 220 186 244 692.0 0 244 187 997 692.0 0 997 188 6775 692.0 0 6775 189 2248 692.0 0 2248 190 4137 692.0 0 4137 191 480 692.0 0 480 192 610 692.0 0 610 193 479 692.0 0 479 194 493 692.0 0 493 195 138 692.0 0 138 196 188 692.0 0 188 197 70 692.0 0 70 198 72 692.0 0 72 199 116 692.0 0 116 200 70 692.0 0 70 201 87 692.0 0 87 202 90 692.0 0 90 203 85 692.0 0 85 204 108 692.0 0 108 205 122 692.0 0 122 206 1073 692.0 0 1073 207 1511 692.0 0 1511 208 2178 692.0 0 2178 209 216 692.0 0 216 210 135 692.0 0 135 211 451 692.0 0 451 212 472 692.0 0 472 213 921 692.0 0 921 214 283 692.0 0 283 215 51 692.0 0 51 216 73 692.0 0 73 217 39 692.0 0 39 218 51 692.0 0 51 219 64 692.0 0 64 220 41 692.0 0 41 221 60 692.0 0 60 222 157 692.0 0 157 223 96 692.0 0 96 224 54 692.0 0 54 225 67 692.0 0 67 226 97 692.0 0 97 227 80 692.0 0 80 228 88 692.0 0 88 229 140 692.0 0 140 230 181 692.0 0 181 231 136 692.0 0 136 232 87 692.0 0 87 233 75 692.0 0 75 234 87 692.0 0 87 235 84 692.0 0 84 236 60 692.0 0 60 237 98 692.0 0 98 238 79 692.0 0 79 239 74 692.0 0 74 240 96 692.0 0 96 241 89 692.0 0 89 242 68 692.0 0 68 243 64 692.0 0 64 244 95 692.0 0 95 245 53 692.0 0 53 246 65 692.0 0 65 247 104 692.0 0 104 248 121 692.0 0 121 249 66 692.0 0 66 250 78 692.0 0 78 251 110 692.0 0 110 252 136 692.0 0 136 253 87 692.0 0 87 254 117 692.0 0 117 255 138 692.0 0 138 256 164 692.0 0 164 257 333 692.0 0 333 258 268 692.0 0 268 259 145 692.0 0 145 260 229 692.0 0 229 261 220 692.0 0 220 262 142 692.0 0 142 263 97 692.0 0 97 264 105 692.0 0 105 265 90 692.0 0 90 266 139 692.0 0 139 267 153 692.0 0 153 268 788 692.0 0 788 269 745 692.0 0 745 270 1555 692.0 0 1555 271 2811 692.0 0 2811 272 3091 692.0 0 3091 273 2860 692.0 0 2860 274 475 692.0 0 475 275 119 692.0 0 119 276 83 692.0 0 83 277 121 692.0 0 121 278 103 692.0 0 103 279 91 692.0 0 91 280 116 692.0 0 116 281 95 692.0 0 95 282 873 692.0 0 873 283 451 692.0 0 451 284 631 692.0 0 631 285 76 692.0 0 76 286 87 692.0 0 87 287 94 692.0 0 94 288 102 692.0 0 102 289 140 692.0 0 140 290 88 692.0 0 88 291 167 692.0 0 167 292 518 692.0 0 518 293 316 692.0 0 316 294 309 692.0 0 309 295 177 692.0 0 177 296 111 692.0 0 111 297 135 692.0 0 135 298 134 692.0 0 134 299 144 692.0 0 144 300 347 692.0 0 347 301 230 692.0 0 230 Finished in 4.97 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 112,824 Reads with adapters: 30,683 (27.2%) Reads that were too short: 227 (0.2%) Reads written (passing filters): 30,456 (27.0%) Total basepairs processed: 26,307,829 bp Total written (filtered): 8,385,662 bp (31.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 30683 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 17 0.0 3 0 0 9 8 16 201 0.0 3 0 177 20 4 17 27594 0.0 3 26153 1199 200 42 18 98 0.0 3 8 50 5 35 19 54 0.0 3 8 18 18 10 20 2163 0.0 3 1797 110 12 244 21 4 0.0 3 1 2 0 1 22 3 0.0 3 0 0 0 3 23 15 0.0 3 0 0 1 14 27 2 0.0 3 0 0 0 2 31 1 0.0 3 0 0 0 1 34 2 0.0 3 1 0 0 1 35 1 0.0 3 0 0 0 1 36 1 0.0 3 1 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 42 3 0.0 3 0 0 0 3 43 4 0.0 3 0 0 0 4 44 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 1 52 1 0.0 3 1 53 5 0.0 3 0 0 0 5 61 2 0.0 3 0 0 0 2 63 3 0.0 3 0 0 0 3 64 13 0.0 3 0 0 0 13 65 8 0.0 3 0 0 0 8 67 4 0.0 3 0 0 0 4 68 2 0.0 3 0 0 1 1 69 4 0.0 3 0 0 0 4 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 73 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 75 11 0.0 3 0 0 0 11 77 1 0.0 3 0 0 0 1 81 3 0.0 3 0 0 0 3 82 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 86 38 0.0 3 0 0 0 38 87 23 0.0 3 0 0 0 23 88 1 0.0 3 0 0 0 1 89 24 0.0 3 0 0 3 21 90 3 0.0 3 0 0 0 3 91 15 0.0 3 0 0 0 15 92 10 0.0 3 0 0 0 10 93 4 0.0 3 0 0 0 4 94 2 0.0 3 0 0 0 2 95 7 0.0 3 0 0 1 6 96 2 0.0 3 0 0 0 2 97 2 0.0 3 0 0 0 2 100 5 0.0 3 0 0 0 5 102 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 104 3 0.0 3 0 0 0 3 105 2 0.0 3 0 0 0 2 106 11 0.0 3 0 0 0 11 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 7 0.0 3 0 0 0 7 110 2 0.0 3 0 0 0 2 111 37 0.0 3 0 0 1 36 112 2 0.0 3 0 0 0 2 113 2 0.0 3 0 0 0 2 114 8 0.0 3 0 0 0 8 115 9 0.0 3 0 0 0 9 116 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 122 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 129 11 0.0 3 0 0 0 11 130 5 0.0 3 0 0 0 5 131 1 0.0 3 0 0 0 1 132 4 0.0 3 0 0 1 3 133 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 136 6 0.0 3 1 0 0 5 137 4 0.0 3 0 0 0 4 139 1 0.0 3 0 0 0 1 142 3 0.0 3 0 0 1 2 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 1 1 151 3 0.0 3 0 0 0 3 154 1 0.0 3 0 0 0 1 155 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 3 0.0 3 0 0 0 3 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 3 0.0 3 0 0 0 3 173 4 0.0 3 0 0 0 4 174 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 4 0.0 3 0 0 0 4 186 2 0.0 3 0 0 0 2 187 4 0.0 3 0 0 0 4 188 5 0.0 3 0 0 1 4 189 3 0.0 3 0 0 0 3 191 11 0.0 3 0 0 0 11 192 5 0.0 3 0 0 0 5 194 4 0.0 3 0 0 0 4 198 2 0.0 3 0 0 0 2 200 1 0.0 3 0 0 0 1 203 2 0.0 3 0 0 0 2 204 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 42 0.0 3 0 0 0 42 207 2 0.0 3 0 0 0 2 208 2 0.0 3 0 0 0 2 211 3 0.0 3 0 0 0 3 212 5 0.0 3 0 0 1 4 213 9 0.0 3 0 0 0 9 215 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 1 218 3 0.0 3 0 0 1 2 243 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 6 0.0 3 0 0 0 6 282 4 0.0 3 0 0 0 4 284 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.08 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 30,456 Reads with adapters: 139 (0.5%) Reads written (passing filters): 30,456 (100.0%) Total basepairs processed: 8,385,662 bp Total written (filtered): 8,385,232 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 139 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 36.0% C: 29.5% G: 30.2% T: 4.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 131 475.9 0 131 4 5 119.0 0 5 5 2 29.7 0 2 7 1 1.9 0 1