This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.23 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 132,497 (4.7%) Reads that were too short: 23,137 (0.8%) Reads written (passing filters): 109,360 (3.9%) Total basepairs processed: 853,219,115 bp Total written (filtered): 25,830,803 bp (3.0%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 132497 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1890 692.0 0 1890 7 23276 692.0 0 23276 8 27664 692.0 0 27664 9 43 692.0 0 43 10 26 692.0 0 26 11 33 692.0 0 33 12 27 692.0 0 27 13 19 692.0 0 19 14 30 692.0 0 30 15 32 692.0 0 32 16 28 692.0 0 28 17 41 692.0 0 41 18 40 692.0 0 40 19 21 692.0 0 21 20 41 692.0 0 41 21 28 692.0 0 28 22 31 692.0 0 31 23 16 692.0 0 16 24 29 692.0 0 29 25 21 692.0 0 21 26 30 692.0 0 30 27 31 692.0 0 31 28 35 692.0 0 35 29 24 692.0 0 24 30 66 692.0 0 66 31 32 692.0 0 32 32 25 692.0 0 25 33 32 692.0 0 32 34 64 692.0 0 64 35 32 692.0 0 32 36 74 692.0 0 74 37 70 692.0 0 70 38 64 692.0 0 64 39 34 692.0 0 34 40 41 692.0 0 41 41 42 692.0 0 42 42 224 692.0 0 224 43 36 692.0 0 36 44 13 692.0 0 13 45 44 692.0 0 44 46 182 692.0 0 182 47 23 692.0 0 23 48 197 692.0 0 197 49 51 692.0 0 51 50 42 692.0 0 42 51 37 692.0 0 37 52 42 692.0 0 42 53 29 692.0 0 29 54 44 692.0 0 44 55 25 692.0 0 25 56 53 692.0 0 53 57 25 692.0 0 25 58 44 692.0 0 44 59 30 692.0 0 30 60 62 692.0 0 62 61 32 692.0 0 32 62 45 692.0 0 45 63 51 692.0 0 51 64 87 692.0 0 87 65 131 692.0 0 131 66 244 692.0 0 244 67 488 692.0 0 488 68 125 692.0 0 125 69 271 692.0 0 271 70 397 692.0 0 397 71 90 692.0 0 90 72 112 692.0 0 112 73 168 692.0 0 168 74 132 692.0 0 132 75 205 692.0 0 205 76 199 692.0 0 199 77 221 692.0 0 221 78 236 692.0 0 236 79 431 692.0 0 431 80 5823 692.0 0 5823 81 8353 692.0 0 8353 82 12246 692.0 0 12246 83 93 692.0 0 93 84 71 692.0 0 71 85 26 692.0 0 26 86 31 692.0 0 31 87 44 692.0 0 44 88 62 692.0 0 62 89 82 692.0 0 82 90 29 692.0 0 29 91 36 692.0 0 36 92 37 692.0 0 37 93 22 692.0 0 22 94 26 692.0 0 26 95 37 692.0 0 37 96 73 692.0 0 73 97 54 692.0 0 54 98 38 692.0 0 38 99 36 692.0 0 36 100 18 692.0 0 18 101 19 692.0 0 19 102 33 692.0 0 33 103 39 692.0 0 39 104 34 692.0 0 34 105 26 692.0 0 26 106 26 692.0 0 26 107 35 692.0 0 35 108 28 692.0 0 28 109 17 692.0 0 17 110 36 692.0 0 36 111 40 692.0 0 40 112 48 692.0 0 48 113 46 692.0 0 46 114 37 692.0 0 37 115 30 692.0 0 30 116 14 692.0 0 14 117 60 692.0 0 60 118 54 692.0 0 54 119 69 692.0 0 69 120 96 692.0 0 96 121 124 692.0 0 124 122 127 692.0 0 127 123 52 692.0 0 52 124 61 692.0 0 61 125 56 692.0 0 56 126 79 692.0 0 79 127 158 692.0 0 158 128 662 692.0 0 662 129 93 692.0 0 93 130 34 692.0 0 34 131 43 692.0 0 43 132 43 692.0 0 43 133 38 692.0 0 38 134 42 692.0 0 42 135 68 692.0 0 68 136 118 692.0 0 118 137 35 692.0 0 35 138 43 692.0 0 43 139 56 692.0 0 56 140 23 692.0 0 23 141 39 692.0 0 39 142 39 692.0 0 39 143 35 692.0 0 35 144 78 692.0 0 78 145 44 692.0 0 44 146 77 692.0 0 77 147 50 692.0 0 50 148 41 692.0 0 41 149 57 692.0 0 57 150 137 692.0 0 137 151 28 692.0 0 28 152 51 692.0 0 51 153 53 692.0 0 53 154 53 692.0 0 53 155 37 692.0 0 37 156 41 692.0 0 41 157 23 692.0 0 23 158 30 692.0 0 30 159 31 692.0 0 31 160 41 692.0 0 41 161 54 692.0 0 54 162 60 692.0 0 60 163 55 692.0 0 55 164 63 692.0 0 63 165 99 692.0 0 99 166 65 692.0 0 65 167 245 692.0 0 245 168 89 692.0 0 89 169 1146 692.0 0 1146 170 47 692.0 0 47 171 62 692.0 0 62 172 58 692.0 0 58 173 76 692.0 0 76 174 142 692.0 0 142 175 235 692.0 0 235 176 87 692.0 0 87 177 122 692.0 0 122 178 103 692.0 0 103 179 86 692.0 0 86 180 74 692.0 0 74 181 103 692.0 0 103 182 96 692.0 0 96 183 89 692.0 0 89 184 106 692.0 0 106 185 152 692.0 0 152 186 207 692.0 0 207 187 910 692.0 0 910 188 6547 692.0 0 6547 189 2277 692.0 0 2277 190 4336 692.0 0 4336 191 341 692.0 0 341 192 422 692.0 0 422 193 247 692.0 0 247 194 266 692.0 0 266 195 86 692.0 0 86 196 109 692.0 0 109 197 36 692.0 0 36 198 40 692.0 0 40 199 54 692.0 0 54 200 43 692.0 0 43 201 61 692.0 0 61 202 51 692.0 0 51 203 64 692.0 0 64 204 67 692.0 0 67 205 84 692.0 0 84 206 882 692.0 0 882 207 1357 692.0 0 1357 208 1978 692.0 0 1978 209 161 692.0 0 161 210 27 692.0 0 27 211 164 692.0 0 164 212 141 692.0 0 141 213 286 692.0 0 286 214 106 692.0 0 106 215 41 692.0 0 41 216 55 692.0 0 55 217 25 692.0 0 25 218 32 692.0 0 32 219 58 692.0 0 58 220 38 692.0 0 38 221 34 692.0 0 34 222 153 692.0 0 153 223 53 692.0 0 53 224 28 692.0 0 28 225 42 692.0 0 42 226 74 692.0 0 74 227 61 692.0 0 61 228 48 692.0 0 48 229 71 692.0 0 71 230 65 692.0 0 65 231 63 692.0 0 63 232 50 692.0 0 50 233 42 692.0 0 42 234 48 692.0 0 48 235 36 692.0 0 36 236 30 692.0 0 30 237 57 692.0 0 57 238 43 692.0 0 43 239 31 692.0 0 31 240 33 692.0 0 33 241 52 692.0 0 52 242 37 692.0 0 37 243 40 692.0 0 40 244 44 692.0 0 44 245 43 692.0 0 43 246 34 692.0 0 34 247 56 692.0 0 56 248 63 692.0 0 63 249 49 692.0 0 49 250 40 692.0 0 40 251 79 692.0 0 79 252 78 692.0 0 78 253 58 692.0 0 58 254 64 692.0 0 64 255 64 692.0 0 64 256 107 692.0 0 107 257 220 692.0 0 220 258 270 692.0 0 270 259 83 692.0 0 83 260 102 692.0 0 102 261 172 692.0 0 172 262 78 692.0 0 78 263 70 692.0 0 70 264 77 692.0 0 77 265 50 692.0 0 50 266 78 692.0 0 78 267 78 692.0 0 78 268 325 692.0 0 325 269 232 692.0 0 232 270 488 692.0 0 488 271 2994 692.0 0 2994 272 3950 692.0 0 3950 273 1736 692.0 0 1736 274 277 692.0 0 277 275 77 692.0 0 77 276 34 692.0 0 34 277 69 692.0 0 69 278 48 692.0 0 48 279 63 692.0 0 63 280 58 692.0 0 58 281 63 692.0 0 63 282 663 692.0 0 663 283 344 692.0 0 344 284 477 692.0 0 477 285 59 692.0 0 59 286 70 692.0 0 70 287 66 692.0 0 66 288 63 692.0 0 63 289 75 692.0 0 75 290 63 692.0 0 63 291 110 692.0 0 110 292 503 692.0 0 503 293 293 692.0 0 293 294 296 692.0 0 296 295 105 692.0 0 105 296 78 692.0 0 78 297 82 692.0 0 82 298 105 692.0 0 105 299 77 692.0 0 77 300 183 692.0 0 183 301 146 692.0 0 146 Finished in 5.41 s (49 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 109,360 Reads with adapters: 28,541 (26.1%) Reads that were too short: 62 (0.1%) Reads written (passing filters): 28,479 (26.0%) Total basepairs processed: 25,830,803 bp Total written (filtered): 7,767,703 bp (30.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 28541 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 4 0.0 3 0 0 0 0 4 18 7 0.0 3 0 0 7 19 279 0.0 3 0 275 1 1 2 20 28012 0.0 4 27550 448 11 3 21 68 0.0 4 16 51 1 22 5 0.0 4 0 1 1 1 2 23 29 0.0 4 0 0 0 26 3 24 1 0.0 4 0 0 0 0 1 27 4 0.0 4 1 0 0 0 3 29 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 55 14 0.0 4 0 0 0 0 14 62 1 0.0 4 0 0 0 0 1 64 1 0.0 4 1 72 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 1 90 1 0.0 4 1 93 3 0.0 4 1 2 94 1 0.0 4 1 111 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 37 0.0 4 0 0 0 0 37 130 7 0.0 4 0 0 0 0 7 131 1 0.0 4 0 0 0 0 1 137 1 0.0 4 1 145 1 0.0 4 0 1 150 1 0.0 4 0 0 0 0 1 155 29 0.0 4 0 0 0 0 29 156 8 0.0 4 0 0 0 0 8 157 2 0.0 4 0 0 0 0 2 165 1 0.0 4 0 0 0 1 172 1 0.0 4 0 1 173 1 0.0 4 0 0 0 0 1 174 1 0.0 4 1 189 1 0.0 4 1 204 1 0.0 4 1 208 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 237 1 0.0 4 1 240 1 0.0 4 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.99 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 28,479 Reads with adapters: 2,488 (8.7%) Reads written (passing filters): 28,479 (100.0%) Total basepairs processed: 7,767,703 bp Total written (filtered): 7,738,015 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2488 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.0% C: 3.3% G: 93.9% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 13 445.0 0 13 4 3 111.2 0 3 5 2 27.8 0 2 9 8 0.1 0 0 8 10 90 0.0 1 5 85 11 2355 0.0 1 20 2335 12 3 0.0 1 0 3 81 1 0.0 1 1 190 1 0.0 1 1 200 9 0.0 1 8 1 209 1 0.0 1 0 1 210 1 0.0 1 1 224 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.