This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.91 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 126,710 (4.5%) Reads that were too short: 59,072 (2.1%) Reads written (passing filters): 67,638 (2.4%) Total basepairs processed: 853,219,115 bp Total written (filtered): 12,307,199 bp (1.4%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 126710 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 130 692.0 0 130 7 5651 692.0 0 5651 8 93 692.0 0 93 9 82 692.0 0 82 10 107 692.0 0 107 11 78 692.0 0 78 12 74 692.0 0 74 13 101 692.0 0 101 14 99 692.0 0 99 15 83 692.0 0 83 16 89 692.0 0 89 17 75 692.0 0 75 18 85 692.0 0 85 19 97 692.0 0 97 20 83 692.0 0 83 21 97 692.0 0 97 22 86 692.0 0 86 23 94 692.0 0 94 24 102 692.0 0 102 25 84 692.0 0 84 26 80 692.0 0 80 27 88 692.0 0 88 28 90 692.0 0 90 29 76 692.0 0 76 30 65 692.0 0 65 31 79 692.0 0 79 32 89 692.0 0 89 33 85 692.0 0 85 34 99 692.0 0 99 35 95 692.0 0 95 36 74 692.0 0 74 37 66 692.0 0 66 38 82 692.0 0 82 39 108 692.0 0 108 40 77 692.0 0 77 41 61 692.0 0 61 42 77 692.0 0 77 43 94 692.0 0 94 44 83 692.0 0 83 45 94 692.0 0 94 46 80 692.0 0 80 47 96 692.0 0 96 48 68 692.0 0 68 49 82 692.0 0 82 50 66 692.0 0 66 51 80 692.0 0 80 52 77 692.0 0 77 53 86 692.0 0 86 54 82 692.0 0 82 55 71 692.0 0 71 56 95 692.0 0 95 57 93 692.0 0 93 58 51 692.0 0 51 59 82 692.0 0 82 60 95 692.0 0 95 61 86 692.0 0 86 62 89 692.0 0 89 63 86 692.0 0 86 64 103 692.0 0 103 65 72 692.0 0 72 66 98 692.0 0 98 67 89 692.0 0 89 68 83 692.0 0 83 69 102 692.0 0 102 70 97 692.0 0 97 71 79 692.0 0 79 72 90 692.0 0 90 73 94 692.0 0 94 74 260 692.0 0 260 75 178 692.0 0 178 76 292 692.0 0 292 77 203 692.0 0 203 78 298 692.0 0 298 79 106 692.0 0 106 80 114 692.0 0 114 81 140 692.0 0 140 82 116 692.0 0 116 83 122 692.0 0 122 84 114 692.0 0 114 85 97 692.0 0 97 86 106 692.0 0 106 87 130 692.0 0 130 88 140 692.0 0 140 89 152 692.0 0 152 90 92 692.0 0 92 91 133 692.0 0 133 92 104 692.0 0 104 93 122 692.0 0 122 94 111 692.0 0 111 95 138 692.0 0 138 96 118 692.0 0 118 97 128 692.0 0 128 98 118 692.0 0 118 99 121 692.0 0 121 100 103 692.0 0 103 101 91 692.0 0 91 102 91 692.0 0 91 103 146 692.0 0 146 104 162 692.0 0 162 105 139 692.0 0 139 106 178 692.0 0 178 107 541 692.0 0 541 108 206 692.0 0 206 109 250 692.0 0 250 110 151 692.0 0 151 111 138 692.0 0 138 112 125 692.0 0 125 113 157 692.0 0 157 114 129 692.0 0 129 115 141 692.0 0 141 116 130 692.0 0 130 117 138 692.0 0 138 118 148 692.0 0 148 119 155 692.0 0 155 120 345 692.0 0 345 121 412 692.0 0 412 122 254 692.0 0 254 123 318 692.0 0 318 124 404 692.0 0 404 125 961 692.0 0 961 126 208 692.0 0 208 127 298 692.0 0 298 128 304 692.0 0 304 129 272 692.0 0 272 130 252 692.0 0 252 131 307 692.0 0 307 132 298 692.0 0 298 133 907 692.0 0 907 134 3190 692.0 0 3190 135 6356 692.0 0 6356 136 14682 692.0 0 14682 137 336 692.0 0 336 138 236 692.0 0 236 139 278 692.0 0 278 140 207 692.0 0 207 141 259 692.0 0 259 142 271 692.0 0 271 143 145 692.0 0 145 144 162 692.0 0 162 145 163 692.0 0 163 146 173 692.0 0 173 147 315 692.0 0 315 148 262 692.0 0 262 149 232 692.0 0 232 150 148 692.0 0 148 151 183 692.0 0 183 152 222 692.0 0 222 153 228 692.0 0 228 154 674 692.0 0 674 155 929 692.0 0 929 156 1414 692.0 0 1414 157 2631 692.0 0 2631 158 1681 692.0 0 1681 159 2759 692.0 0 2759 160 162 692.0 0 162 161 209 692.0 0 209 162 175 692.0 0 175 163 127 692.0 0 127 164 174 692.0 0 174 165 160 692.0 0 160 166 202 692.0 0 202 167 154 692.0 0 154 168 110 692.0 0 110 169 167 692.0 0 167 170 92 692.0 0 92 171 110 692.0 0 110 172 104 692.0 0 104 173 137 692.0 0 137 174 175 692.0 0 175 175 131 692.0 0 131 176 104 692.0 0 104 177 90 692.0 0 90 178 84 692.0 0 84 179 87 692.0 0 87 180 129 692.0 0 129 181 138 692.0 0 138 182 125 692.0 0 125 183 124 692.0 0 124 184 111 692.0 0 111 185 115 692.0 0 115 186 244 692.0 0 244 187 122 692.0 0 122 188 133 692.0 0 133 189 100 692.0 0 100 190 119 692.0 0 119 191 143 692.0 0 143 192 129 692.0 0 129 193 120 692.0 0 120 194 105 692.0 0 105 195 70 692.0 0 70 196 91 692.0 0 91 197 110 692.0 0 110 198 80 692.0 0 80 199 116 692.0 0 116 200 132 692.0 0 132 201 156 692.0 0 156 202 169 692.0 0 169 203 141 692.0 0 141 204 119 692.0 0 119 205 102 692.0 0 102 206 116 692.0 0 116 207 131 692.0 0 131 208 111 692.0 0 111 209 127 692.0 0 127 210 103 692.0 0 103 211 95 692.0 0 95 212 114 692.0 0 114 213 206 692.0 0 206 214 155 692.0 0 155 215 155 692.0 0 155 216 152 692.0 0 152 217 111 692.0 0 111 218 129 692.0 0 129 219 151 692.0 0 151 220 159 692.0 0 159 221 164 692.0 0 164 222 164 692.0 0 164 223 100 692.0 0 100 224 105 692.0 0 105 225 206 692.0 0 206 226 137 692.0 0 137 227 117 692.0 0 117 228 151 692.0 0 151 229 123 692.0 0 123 230 136 692.0 0 136 231 128 692.0 0 128 232 179 692.0 0 179 233 149 692.0 0 149 234 189 692.0 0 189 235 119 692.0 0 119 236 94 692.0 0 94 237 113 692.0 0 113 238 106 692.0 0 106 239 107 692.0 0 107 240 113 692.0 0 113 241 134 692.0 0 134 242 128 692.0 0 128 243 141 692.0 0 141 244 130 692.0 0 130 245 117 692.0 0 117 246 118 692.0 0 118 247 126 692.0 0 126 248 115 692.0 0 115 249 90 692.0 0 90 250 124 692.0 0 124 251 214 692.0 0 214 252 406 692.0 0 406 253 264 692.0 0 264 254 250 692.0 0 250 255 116 692.0 0 116 256 125 692.0 0 125 257 148 692.0 0 148 258 124 692.0 0 124 259 148 692.0 0 148 260 136 692.0 0 136 261 129 692.0 0 129 262 158 692.0 0 158 263 135 692.0 0 135 264 135 692.0 0 135 265 106 692.0 0 106 266 132 692.0 0 132 267 144 692.0 0 144 268 237 692.0 0 237 269 186 692.0 0 186 270 503 692.0 0 503 271 811 692.0 0 811 272 763 692.0 0 763 273 258 692.0 0 258 274 654 692.0 0 654 275 274 692.0 0 274 276 1619 692.0 0 1619 277 1405 692.0 0 1405 278 1624 692.0 0 1624 279 510 692.0 0 510 280 292 692.0 0 292 281 448 692.0 0 448 282 647 692.0 0 647 283 209 692.0 0 209 284 269 692.0 0 269 285 414 692.0 0 414 286 221 692.0 0 221 287 210 692.0 0 210 288 368 692.0 0 368 289 522 692.0 0 522 290 473 692.0 0 473 291 394 692.0 0 394 292 420 692.0 0 420 293 476 692.0 0 476 294 681 692.0 0 681 295 15506 692.0 0 15506 296 8652 692.0 0 8652 297 10181 692.0 0 10181 298 135 692.0 0 135 299 89 692.0 0 89 300 118 692.0 0 118 301 164 692.0 0 164 Finished in 2.77 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 67,638 Reads with adapters: 3,258 (4.8%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 3,240 (4.8%) Total basepairs processed: 12,307,199 bp Total written (filtered): 887,597 bp (7.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3258 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 17 0.0 3 0 15 0 1 1 20 3213 0.0 4 2884 317 11 0 1 21 6 0.0 4 1 4 0 1 22 1 0.0 4 1 23 1 0.0 4 0 0 0 1 38 1 0.0 4 1 40 1 0.0 4 0 0 1 42 1 0.0 4 0 0 0 0 1 81 4 0.0 4 0 0 0 0 4 126 2 0.0 4 0 0 0 0 2 129 1 0.0 4 1 132 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 1 188 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 3,240 Reads with adapters: 29 (0.9%) Reads written (passing filters): 3,240 (100.0%) Total basepairs processed: 887,597 bp Total written (filtered): 887,175 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 29 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 17.2% C: 0.0% G: 72.4% T: 10.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5 50.6 0 5 10 2 0.0 1 0 2 11 17 0.0 1 0 17 12 4 0.0 1 0 4 152 1 0.0 1 0 1