This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.62 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 76,290 (2.7%) Reads that were too short: 28,997 (1.0%) Reads written (passing filters): 47,293 (1.7%) Total basepairs processed: 853,219,115 bp Total written (filtered): 9,020,401 bp (1.1%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 76290 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 354 692.0 0 354 7 249 692.0 0 249 8 6185 692.0 0 6185 9 4516 692.0 0 4516 10 18 692.0 0 18 11 3 692.0 0 3 12 5 692.0 0 5 13 22 692.0 0 22 14 70 692.0 0 70 15 197 692.0 0 197 16 31 692.0 0 31 17 17 692.0 0 17 18 48 692.0 0 48 19 59 692.0 0 59 20 43 692.0 0 43 21 14 692.0 0 14 22 1 692.0 0 1 24 2 692.0 0 2 25 2 692.0 0 2 26 2 692.0 0 2 27 9 692.0 0 9 28 9 692.0 0 9 29 10 692.0 0 10 31 2 692.0 0 2 32 6 692.0 0 6 33 10 692.0 0 10 34 13 692.0 0 13 35 6 692.0 0 6 36 7 692.0 0 7 37 5 692.0 0 5 38 10 692.0 0 10 39 4 692.0 0 4 40 9 692.0 0 9 41 10 692.0 0 10 42 15 692.0 0 15 43 96 692.0 0 96 44 333 692.0 0 333 45 364 692.0 0 364 46 101 692.0 0 101 47 15 692.0 0 15 48 12 692.0 0 12 49 10 692.0 0 10 50 17 692.0 0 17 51 16 692.0 0 16 52 17 692.0 0 17 53 21 692.0 0 21 54 11 692.0 0 11 55 7 692.0 0 7 56 24 692.0 0 24 57 13 692.0 0 13 58 16 692.0 0 16 59 66 692.0 0 66 60 59 692.0 0 59 61 30 692.0 0 30 62 17 692.0 0 17 63 9 692.0 0 9 64 10 692.0 0 10 65 36 692.0 0 36 66 35 692.0 0 35 67 35 692.0 0 35 68 82 692.0 0 82 69 36 692.0 0 36 70 28 692.0 0 28 71 51 692.0 0 51 72 71 692.0 0 71 73 43 692.0 0 43 74 74 692.0 0 74 75 101 692.0 0 101 76 223 692.0 0 223 77 2207 692.0 0 2207 78 2539 692.0 0 2539 79 4379 692.0 0 4379 80 45 692.0 0 45 81 18 692.0 0 18 82 38 692.0 0 38 83 17 692.0 0 17 84 31 692.0 0 31 85 28 692.0 0 28 86 49 692.0 0 49 87 6 692.0 0 6 88 4 692.0 0 4 89 7 692.0 0 7 90 13 692.0 0 13 91 33 692.0 0 33 92 27 692.0 0 27 93 37 692.0 0 37 94 53 692.0 0 53 95 22 692.0 0 22 96 32 692.0 0 32 97 7 692.0 0 7 98 5 692.0 0 5 99 8 692.0 0 8 100 7 692.0 0 7 101 10 692.0 0 10 102 15 692.0 0 15 103 44 692.0 0 44 104 23 692.0 0 23 105 10 692.0 0 10 106 11 692.0 0 11 107 6 692.0 0 6 108 39 692.0 0 39 109 54 692.0 0 54 110 7 692.0 0 7 111 13 692.0 0 13 112 13 692.0 0 13 113 23 692.0 0 23 114 74 692.0 0 74 115 54 692.0 0 54 116 144 692.0 0 144 117 108 692.0 0 108 118 21 692.0 0 21 119 17 692.0 0 17 120 25 692.0 0 25 121 30 692.0 0 30 122 24 692.0 0 24 123 37 692.0 0 37 124 24 692.0 0 24 125 32 692.0 0 32 126 20 692.0 0 20 127 43 692.0 0 43 128 28 692.0 0 28 129 13 692.0 0 13 130 22 692.0 0 22 131 10 692.0 0 10 132 12 692.0 0 12 133 31 692.0 0 31 134 45 692.0 0 45 135 123 692.0 0 123 136 184 692.0 0 184 137 142 692.0 0 142 138 77 692.0 0 77 139 24 692.0 0 24 140 11 692.0 0 11 141 28 692.0 0 28 142 46 692.0 0 46 143 29 692.0 0 29 144 42 692.0 0 42 145 19 692.0 0 19 146 12 692.0 0 12 147 16 692.0 0 16 148 25 692.0 0 25 149 19 692.0 0 19 150 70 692.0 0 70 151 20 692.0 0 20 152 98 692.0 0 98 153 26 692.0 0 26 154 30 692.0 0 30 155 26 692.0 0 26 156 16 692.0 0 16 157 18 692.0 0 18 158 22 692.0 0 22 159 25 692.0 0 25 160 52 692.0 0 52 161 32 692.0 0 32 162 37 692.0 0 37 163 82 692.0 0 82 164 129 692.0 0 129 165 85 692.0 0 85 166 83 692.0 0 83 167 45 692.0 0 45 168 47 692.0 0 47 169 57 692.0 0 57 170 79 692.0 0 79 171 83 692.0 0 83 172 180 692.0 0 180 173 1454 692.0 0 1454 174 1050 692.0 0 1050 175 3789 692.0 0 3789 176 90 692.0 0 90 177 45 692.0 0 45 178 60 692.0 0 60 179 146 692.0 0 146 180 1149 692.0 0 1149 181 378 692.0 0 378 182 583 692.0 0 583 183 38 692.0 0 38 184 55 692.0 0 55 185 77 692.0 0 77 186 94 692.0 0 94 187 65 692.0 0 65 188 104 692.0 0 104 189 212 692.0 0 212 190 494 692.0 0 494 191 6551 692.0 0 6551 192 1471 692.0 0 1471 193 2136 692.0 0 2136 194 151 692.0 0 151 195 18 692.0 0 18 196 13 692.0 0 13 197 29 692.0 0 29 198 45 692.0 0 45 199 65 692.0 0 65 200 30 692.0 0 30 201 26 692.0 0 26 202 232 692.0 0 232 203 356 692.0 0 356 204 243 692.0 0 243 205 137 692.0 0 137 206 38 692.0 0 38 207 31 692.0 0 31 208 220 692.0 0 220 209 232 692.0 0 232 210 286 692.0 0 286 211 73 692.0 0 73 212 26 692.0 0 26 213 25 692.0 0 25 214 18 692.0 0 18 215 39 692.0 0 39 216 32 692.0 0 32 217 44 692.0 0 44 218 90 692.0 0 90 219 27 692.0 0 27 220 41 692.0 0 41 221 16 692.0 0 16 222 11 692.0 0 11 223 12 692.0 0 12 224 22 692.0 0 22 225 26 692.0 0 26 226 26 692.0 0 26 227 39 692.0 0 39 228 18 692.0 0 18 229 48 692.0 0 48 230 24 692.0 0 24 231 53 692.0 0 53 232 29 692.0 0 29 233 31 692.0 0 31 234 62 692.0 0 62 235 45 692.0 0 45 236 31 692.0 0 31 237 29 692.0 0 29 238 20 692.0 0 20 239 15 692.0 0 15 240 37 692.0 0 37 241 23 692.0 0 23 242 48 692.0 0 48 243 34 692.0 0 34 244 39 692.0 0 39 245 29 692.0 0 29 246 41 692.0 0 41 247 49 692.0 0 49 248 68 692.0 0 68 249 48 692.0 0 48 250 45 692.0 0 45 251 153 692.0 0 153 252 251 692.0 0 251 253 316 692.0 0 316 254 435 692.0 0 435 255 67 692.0 0 67 256 30 692.0 0 30 257 52 692.0 0 52 258 155 692.0 0 155 259 617 692.0 0 617 260 812 692.0 0 812 261 712 692.0 0 712 262 240 692.0 0 240 263 142 692.0 0 142 264 264 692.0 0 264 265 288 692.0 0 288 266 141 692.0 0 141 267 78 692.0 0 78 268 59 692.0 0 59 269 25 692.0 0 25 270 70 692.0 0 70 271 132 692.0 0 132 272 116 692.0 0 116 273 115 692.0 0 115 274 99 692.0 0 99 275 73 692.0 0 73 276 66 692.0 0 66 277 105 692.0 0 105 278 147 692.0 0 147 279 173 692.0 0 173 280 155 692.0 0 155 281 144 692.0 0 144 282 180 692.0 0 180 283 404 692.0 0 404 284 4170 692.0 0 4170 285 3448 692.0 0 3448 286 10023 692.0 0 10023 287 102 692.0 0 102 288 79 692.0 0 79 289 69 692.0 0 69 290 47 692.0 0 47 291 60 692.0 0 60 292 48 692.0 0 48 293 40 692.0 0 40 294 55 692.0 0 55 295 101 692.0 0 101 296 125 692.0 0 125 297 290 692.0 0 290 298 102 692.0 0 102 299 48 692.0 0 48 300 40 692.0 0 40 301 126 692.0 0 126 Finished in 1.79 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 47,293 Reads with adapters: 6,371 (13.5%) Reads that were too short: 129 (0.3%) Reads written (passing filters): 6,242 (13.2%) Total basepairs processed: 9,020,401 bp Total written (filtered): 1,671,449 bp (18.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6371 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 7 0.0 3 0 0 3 4 16 43 0.0 3 0 29 10 4 17 5422 0.0 3 5033 315 55 19 18 340 0.0 3 0 13 12 315 19 34 0.0 3 4 7 12 11 20 274 0.0 3 218 23 3 30 21 48 0.0 3 0 0 3 45 23 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 27 2 0.0 3 0 0 0 2 28 1 0.0 3 0 0 1 29 3 0.0 3 1 1 0 1 30 6 0.0 3 0 0 0 6 33 3 0.0 3 0 0 0 3 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 39 3 0.0 3 0 0 0 3 40 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 1 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 53 3 0.0 3 0 0 0 3 54 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 1 57 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 1 1 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 67 1 0.0 3 1 69 4 0.0 3 0 0 1 3 70 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 78 3 0.0 3 0 0 0 3 81 1 0.0 3 1 83 3 0.0 3 0 0 0 3 85 1 0.0 3 0 0 0 1 90 3 0.0 3 0 0 0 3 94 2 0.0 3 1 0 0 1 95 1 0.0 3 0 0 0 1 97 5 0.0 3 0 0 0 5 98 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 106 2 0.0 3 0 0 0 2 107 2 0.0 3 0 0 1 1 109 4 0.0 3 0 0 0 4 110 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 25 0.0 3 0 0 1 24 120 1 0.0 3 0 0 0 1 122 2 0.0 3 1 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 128 4 0.0 3 0 0 0 4 129 2 0.0 3 0 0 0 2 130 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 1 133 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 3 0.0 3 0 0 0 3 153 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 3 0.0 3 0 0 0 3 177 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 1 1 192 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 200 3 0.0 3 0 0 0 3 203 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 14 0.0 3 0 0 0 14 218 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 249 4 0.0 3 0 0 0 4 250 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.21 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 6,242 Reads with adapters: 35 (0.6%) Reads written (passing filters): 6,242 (100.0%) Total basepairs processed: 1,671,449 bp Total written (filtered): 1,671,340 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 35 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 28.6% C: 45.7% G: 14.3% T: 11.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 33 97.5 0 33 4 1 24.4 0 1 6 1 1.5 0 1