This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.48 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 54,135 (1.9%) Reads that were too short: 12,890 (0.5%) Reads written (passing filters): 41,245 (1.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 8,123,436 bp (1.0%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 54135 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 313 692.0 0 313 7 208 692.0 0 208 8 5841 692.0 0 5841 9 5189 692.0 0 5189 10 15 692.0 0 15 11 1 692.0 0 1 13 2 692.0 0 2 14 1 692.0 0 1 15 3 692.0 0 3 16 3 692.0 0 3 17 2 692.0 0 2 18 23 692.0 0 23 19 19 692.0 0 19 20 11 692.0 0 11 21 5 692.0 0 5 22 2 692.0 0 2 24 2 692.0 0 2 27 2 692.0 0 2 28 8 692.0 0 8 29 6 692.0 0 6 30 1 692.0 0 1 31 5 692.0 0 5 32 2 692.0 0 2 33 13 692.0 0 13 34 6 692.0 0 6 35 9 692.0 0 9 36 7 692.0 0 7 37 17 692.0 0 17 38 18 692.0 0 18 39 6 692.0 0 6 40 11 692.0 0 11 41 11 692.0 0 11 42 8 692.0 0 8 43 18 692.0 0 18 44 45 692.0 0 45 45 57 692.0 0 57 46 32 692.0 0 32 47 9 692.0 0 9 48 10 692.0 0 10 49 14 692.0 0 14 50 28 692.0 0 28 51 17 692.0 0 17 52 15 692.0 0 15 53 10 692.0 0 10 54 12 692.0 0 12 55 7 692.0 0 7 56 34 692.0 0 34 57 10 692.0 0 10 58 13 692.0 0 13 59 51 692.0 0 51 60 49 692.0 0 49 61 9 692.0 0 9 62 17 692.0 0 17 63 8 692.0 0 8 64 12 692.0 0 12 65 22 692.0 0 22 66 35 692.0 0 35 67 19 692.0 0 19 68 42 692.0 0 42 69 26 692.0 0 26 70 26 692.0 0 26 71 39 692.0 0 39 72 68 692.0 0 68 73 45 692.0 0 45 74 77 692.0 0 77 75 116 692.0 0 116 76 229 692.0 0 229 77 2457 692.0 0 2457 78 2684 692.0 0 2684 79 4445 692.0 0 4445 80 45 692.0 0 45 81 8 692.0 0 8 82 50 692.0 0 50 83 10 692.0 0 10 84 11 692.0 0 11 85 6 692.0 0 6 86 11 692.0 0 11 87 11 692.0 0 11 88 3 692.0 0 3 89 4 692.0 0 4 90 10 692.0 0 10 91 16 692.0 0 16 92 17 692.0 0 17 93 18 692.0 0 18 94 43 692.0 0 43 95 34 692.0 0 34 96 18 692.0 0 18 97 8 692.0 0 8 98 6 692.0 0 6 99 8 692.0 0 8 100 5 692.0 0 5 101 11 692.0 0 11 102 8 692.0 0 8 103 38 692.0 0 38 104 26 692.0 0 26 105 20 692.0 0 20 106 12 692.0 0 12 107 13 692.0 0 13 108 30 692.0 0 30 109 39 692.0 0 39 110 5 692.0 0 5 111 13 692.0 0 13 112 11 692.0 0 11 113 10 692.0 0 10 114 77 692.0 0 77 115 34 692.0 0 34 116 166 692.0 0 166 117 91 692.0 0 91 118 22 692.0 0 22 119 16 692.0 0 16 120 18 692.0 0 18 121 12 692.0 0 12 122 13 692.0 0 13 123 33 692.0 0 33 124 17 692.0 0 17 125 10 692.0 0 10 126 16 692.0 0 16 127 17 692.0 0 17 128 3 692.0 0 3 129 6 692.0 0 6 130 14 692.0 0 14 131 16 692.0 0 16 132 9 692.0 0 9 133 22 692.0 0 22 134 25 692.0 0 25 135 51 692.0 0 51 136 129 692.0 0 129 137 81 692.0 0 81 138 67 692.0 0 67 139 23 692.0 0 23 140 14 692.0 0 14 141 11 692.0 0 11 142 16 692.0 0 16 143 19 692.0 0 19 144 33 692.0 0 33 145 5 692.0 0 5 146 10 692.0 0 10 147 14 692.0 0 14 148 13 692.0 0 13 149 10 692.0 0 10 150 38 692.0 0 38 151 19 692.0 0 19 152 99 692.0 0 99 153 11 692.0 0 11 154 15 692.0 0 15 155 16 692.0 0 16 156 6 692.0 0 6 157 20 692.0 0 20 158 10 692.0 0 10 159 14 692.0 0 14 160 22 692.0 0 22 161 17 692.0 0 17 162 32 692.0 0 32 163 52 692.0 0 52 164 64 692.0 0 64 165 42 692.0 0 42 166 35 692.0 0 35 167 25 692.0 0 25 168 25 692.0 0 25 169 36 692.0 0 36 170 22 692.0 0 22 171 37 692.0 0 37 172 71 692.0 0 71 173 724 692.0 0 724 174 513 692.0 0 513 175 1620 692.0 0 1620 176 76 692.0 0 76 177 30 692.0 0 30 178 57 692.0 0 57 179 75 692.0 0 75 180 638 692.0 0 638 181 195 692.0 0 195 182 438 692.0 0 438 183 17 692.0 0 17 184 34 692.0 0 34 185 38 692.0 0 38 186 61 692.0 0 61 187 56 692.0 0 56 188 65 692.0 0 65 189 188 692.0 0 188 190 409 692.0 0 409 191 7262 692.0 0 7262 192 1285 692.0 0 1285 193 2222 692.0 0 2222 194 129 692.0 0 129 195 10 692.0 0 10 196 5 692.0 0 5 197 15 692.0 0 15 198 12 692.0 0 12 199 27 692.0 0 27 200 16 692.0 0 16 201 12 692.0 0 12 202 153 692.0 0 153 203 154 692.0 0 154 204 118 692.0 0 118 205 60 692.0 0 60 206 28 692.0 0 28 207 15 692.0 0 15 208 118 692.0 0 118 209 34 692.0 0 34 210 56 692.0 0 56 211 19 692.0 0 19 212 16 692.0 0 16 213 12 692.0 0 12 214 8 692.0 0 8 215 40 692.0 0 40 216 11 692.0 0 11 217 12 692.0 0 12 218 25 692.0 0 25 219 11 692.0 0 11 220 13 692.0 0 13 221 9 692.0 0 9 222 2 692.0 0 2 223 8 692.0 0 8 224 13 692.0 0 13 225 30 692.0 0 30 226 10 692.0 0 10 227 9 692.0 0 9 228 12 692.0 0 12 229 19 692.0 0 19 230 15 692.0 0 15 231 71 692.0 0 71 232 15 692.0 0 15 233 14 692.0 0 14 234 21 692.0 0 21 235 23 692.0 0 23 236 20 692.0 0 20 237 13 692.0 0 13 238 18 692.0 0 18 239 15 692.0 0 15 240 20 692.0 0 20 241 19 692.0 0 19 242 21 692.0 0 21 243 12 692.0 0 12 244 17 692.0 0 17 245 14 692.0 0 14 246 15 692.0 0 15 247 24 692.0 0 24 248 30 692.0 0 30 249 20 692.0 0 20 250 21 692.0 0 21 251 57 692.0 0 57 252 121 692.0 0 121 253 133 692.0 0 133 254 179 692.0 0 179 255 25 692.0 0 25 256 26 692.0 0 26 257 45 692.0 0 45 258 130 692.0 0 130 259 713 692.0 0 713 260 412 692.0 0 412 261 510 692.0 0 510 262 111 692.0 0 111 263 58 692.0 0 58 264 113 692.0 0 113 265 154 692.0 0 154 266 69 692.0 0 69 267 40 692.0 0 40 268 15 692.0 0 15 269 9 692.0 0 9 270 25 692.0 0 25 271 36 692.0 0 36 272 36 692.0 0 36 273 27 692.0 0 27 274 35 692.0 0 35 275 30 692.0 0 30 276 20 692.0 0 20 277 34 692.0 0 34 278 54 692.0 0 54 279 47 692.0 0 47 280 53 692.0 0 53 281 29 692.0 0 29 282 52 692.0 0 52 283 127 692.0 0 127 284 1744 692.0 0 1744 285 1372 692.0 0 1372 286 4192 692.0 0 4192 287 40 692.0 0 40 288 105 692.0 0 105 289 52 692.0 0 52 290 21 692.0 0 21 291 36 692.0 0 36 292 21 692.0 0 21 293 11 692.0 0 11 294 13 692.0 0 13 295 40 692.0 0 40 296 44 692.0 0 44 297 118 692.0 0 118 298 21 692.0 0 21 299 6 692.0 0 6 300 10 692.0 0 10 301 66 692.0 0 66 Finished in 1.71 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 41,245 Reads with adapters: 5,607 (13.6%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 5,594 (13.6%) Total basepairs processed: 8,123,436 bp Total written (filtered): 1,521,172 bp (18.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5607 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 17 0.0 3 0 0 0 0 17 18 3 0.0 3 0 0 2 0 1 19 131 0.0 3 0 127 4 20 5412 0.0 4 5290 112 8 2 21 20 0.0 4 6 14 22 1 0.0 4 0 0 1 23 3 0.0 4 0 0 0 2 1 66 1 0.0 4 1 83 1 0.0 4 0 1 127 2 0.0 4 0 0 0 0 2 135 2 0.0 4 1 0 1 136 1 0.0 4 1 137 2 0.0 4 1 1 155 2 0.0 4 0 0 0 0 2 157 5 0.0 4 0 0 0 0 5 158 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.21 s (38 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 5,594 Reads with adapters: 744 (13.3%) Reads written (passing filters): 5,594 (100.0%) Total basepairs processed: 1,521,172 bp Total written (filtered): 1,512,969 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 744 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.7% C: 3.0% G: 93.4% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 87.4 0 4 4 1 21.9 0 1 5 1 5.5 0 1 9 285 0.0 0 8 277 10 390 0.0 1 18 372 11 54 0.0 1 0 54 12 4 0.0 1 0 4 208 3 0.0 1 2 1 223 1 0.0 1 1 228 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.