This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.12 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 156,680 (5.5%) Reads that were too short: 13,394 (0.5%) Reads written (passing filters): 143,286 (5.1%) Total basepairs processed: 853,219,115 bp Total written (filtered): 40,904,744 bp (4.8%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 156680 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1333 692.0 0 1333 7 73826 692.0 0 73826 8 57360 692.0 0 57360 9 57 692.0 0 57 10 3 692.0 0 3 11 4 692.0 0 4 12 7 692.0 0 7 13 16 692.0 0 16 14 11 692.0 0 11 15 11 692.0 0 11 16 7 692.0 0 7 17 13 692.0 0 13 18 69 692.0 0 69 19 35 692.0 0 35 20 27 692.0 0 27 21 6 692.0 0 6 22 7 692.0 0 7 23 25 692.0 0 25 24 5 692.0 0 5 25 5 692.0 0 5 26 29 692.0 0 29 27 34 692.0 0 34 28 42 692.0 0 42 29 29 692.0 0 29 30 25 692.0 0 25 31 7 692.0 0 7 32 10 692.0 0 10 33 44 692.0 0 44 34 26 692.0 0 26 35 32 692.0 0 32 36 107 692.0 0 107 37 107 692.0 0 107 38 525 692.0 0 525 39 15 692.0 0 15 40 17 692.0 0 17 41 12 692.0 0 12 42 10 692.0 0 10 43 154 692.0 0 154 44 177 692.0 0 177 45 652 692.0 0 652 46 25 692.0 0 25 47 8 692.0 0 8 48 7 692.0 0 7 49 21 692.0 0 21 50 11 692.0 0 11 51 10 692.0 0 10 52 11 692.0 0 11 53 13 692.0 0 13 54 7 692.0 0 7 55 12 692.0 0 12 56 18 692.0 0 18 57 66 692.0 0 66 58 12 692.0 0 12 59 19 692.0 0 19 60 8 692.0 0 8 61 14 692.0 0 14 62 15 692.0 0 15 63 12 692.0 0 12 64 19 692.0 0 19 65 12 692.0 0 12 66 17 692.0 0 17 67 8 692.0 0 8 68 19 692.0 0 19 69 8 692.0 0 8 70 19 692.0 0 19 71 70 692.0 0 70 72 17 692.0 0 17 73 47 692.0 0 47 74 22 692.0 0 22 75 28 692.0 0 28 76 15 692.0 0 15 77 15 692.0 0 15 78 18 692.0 0 18 79 43 692.0 0 43 80 39 692.0 0 39 81 18 692.0 0 18 82 35 692.0 0 35 83 41 692.0 0 41 84 18 692.0 0 18 85 30 692.0 0 30 86 28 692.0 0 28 87 23 692.0 0 23 88 12 692.0 0 12 89 33 692.0 0 33 90 25 692.0 0 25 91 30 692.0 0 30 92 18 692.0 0 18 93 24 692.0 0 24 94 44 692.0 0 44 95 24 692.0 0 24 96 50 692.0 0 50 97 65 692.0 0 65 98 16 692.0 0 16 99 14 692.0 0 14 100 3 692.0 0 3 101 7 692.0 0 7 102 9 692.0 0 9 103 15 692.0 0 15 104 20 692.0 0 20 105 24 692.0 0 24 106 22 692.0 0 22 107 25 692.0 0 25 108 30 692.0 0 30 109 25 692.0 0 25 110 19 692.0 0 19 111 25 692.0 0 25 112 33 692.0 0 33 113 41 692.0 0 41 114 28 692.0 0 28 115 30 692.0 0 30 116 26 692.0 0 26 117 23 692.0 0 23 118 16 692.0 0 16 119 20 692.0 0 20 120 47 692.0 0 47 121 45 692.0 0 45 122 55 692.0 0 55 123 27 692.0 0 27 124 38 692.0 0 38 125 29 692.0 0 29 126 71 692.0 0 71 127 63 692.0 0 63 128 196 692.0 0 196 129 63 692.0 0 63 130 68 692.0 0 68 131 57 692.0 0 57 132 41 692.0 0 41 133 132 692.0 0 132 134 141 692.0 0 141 135 1182 692.0 0 1182 136 238 692.0 0 238 137 438 692.0 0 438 138 49 692.0 0 49 139 56 692.0 0 56 140 98 692.0 0 98 141 148 692.0 0 148 142 132 692.0 0 132 143 63 692.0 0 63 144 45 692.0 0 45 145 38 692.0 0 38 146 57 692.0 0 57 147 96 692.0 0 96 148 201 692.0 0 201 149 376 692.0 0 376 150 233 692.0 0 233 151 55 692.0 0 55 152 27 692.0 0 27 153 59 692.0 0 59 154 33 692.0 0 33 155 46 692.0 0 46 156 56 692.0 0 56 157 30 692.0 0 30 158 31 692.0 0 31 159 23 692.0 0 23 160 35 692.0 0 35 161 50 692.0 0 50 162 30 692.0 0 30 163 45 692.0 0 45 164 49 692.0 0 49 165 37 692.0 0 37 166 38 692.0 0 38 167 63 692.0 0 63 168 37 692.0 0 37 169 30 692.0 0 30 170 26 692.0 0 26 171 28 692.0 0 28 172 30 692.0 0 30 173 35 692.0 0 35 174 39 692.0 0 39 175 53 692.0 0 53 176 48 692.0 0 48 177 38 692.0 0 38 178 37 692.0 0 37 179 24 692.0 0 24 180 72 692.0 0 72 181 42 692.0 0 42 182 77 692.0 0 77 183 29 692.0 0 29 184 40 692.0 0 40 185 46 692.0 0 46 186 49 692.0 0 49 187 53 692.0 0 53 188 38 692.0 0 38 189 35 692.0 0 35 190 54 692.0 0 54 191 51 692.0 0 51 192 40 692.0 0 40 193 52 692.0 0 52 194 66 692.0 0 66 195 66 692.0 0 66 196 45 692.0 0 45 197 37 692.0 0 37 198 31 692.0 0 31 199 36 692.0 0 36 200 48 692.0 0 48 201 49 692.0 0 49 202 64 692.0 0 64 203 45 692.0 0 45 204 26 692.0 0 26 205 30 692.0 0 30 206 40 692.0 0 40 207 80 692.0 0 80 208 130 692.0 0 130 209 277 692.0 0 277 210 96 692.0 0 96 211 46 692.0 0 46 212 118 692.0 0 118 213 100 692.0 0 100 214 169 692.0 0 169 215 203 692.0 0 203 216 107 692.0 0 107 217 62 692.0 0 62 218 37 692.0 0 37 219 81 692.0 0 81 220 79 692.0 0 79 221 86 692.0 0 86 222 80 692.0 0 80 223 42 692.0 0 42 224 45 692.0 0 45 225 48 692.0 0 48 226 69 692.0 0 69 227 50 692.0 0 50 228 86 692.0 0 86 229 72 692.0 0 72 230 133 692.0 0 133 231 69 692.0 0 69 232 86 692.0 0 86 233 195 692.0 0 195 234 153 692.0 0 153 235 359 692.0 0 359 236 85 692.0 0 85 237 46 692.0 0 46 238 68 692.0 0 68 239 34 692.0 0 34 240 53 692.0 0 53 241 44 692.0 0 44 242 61 692.0 0 61 243 80 692.0 0 80 244 71 692.0 0 71 245 51 692.0 0 51 246 63 692.0 0 63 247 33 692.0 0 33 248 51 692.0 0 51 249 47 692.0 0 47 250 46 692.0 0 46 251 42 692.0 0 42 252 49 692.0 0 49 253 48 692.0 0 48 254 52 692.0 0 52 255 65 692.0 0 65 256 71 692.0 0 71 257 51 692.0 0 51 258 63 692.0 0 63 259 71 692.0 0 71 260 88 692.0 0 88 261 66 692.0 0 66 262 84 692.0 0 84 263 56 692.0 0 56 264 82 692.0 0 82 265 64 692.0 0 64 266 57 692.0 0 57 267 90 692.0 0 90 268 171 692.0 0 171 269 673 692.0 0 673 270 301 692.0 0 301 271 601 692.0 0 601 272 123 692.0 0 123 273 136 692.0 0 136 274 240 692.0 0 240 275 120 692.0 0 120 276 140 692.0 0 140 277 98 692.0 0 98 278 97 692.0 0 97 279 97 692.0 0 97 280 174 692.0 0 174 281 252 692.0 0 252 282 204 692.0 0 204 283 136 692.0 0 136 284 67 692.0 0 67 285 81 692.0 0 81 286 65 692.0 0 65 287 85 692.0 0 85 288 131 692.0 0 131 289 80 692.0 0 80 290 124 692.0 0 124 291 116 692.0 0 116 292 130 692.0 0 130 293 280 692.0 0 280 294 542 692.0 0 542 295 544 692.0 0 544 296 1159 692.0 0 1159 297 455 692.0 0 455 298 246 692.0 0 246 299 71 692.0 0 71 300 117 692.0 0 117 301 243 692.0 0 243 Finished in 6.72 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 143,286 Reads with adapters: 72,337 (50.5%) Reads that were too short: 114 (0.1%) Reads written (passing filters): 72,223 (50.4%) Total basepairs processed: 40,904,744 bp Total written (filtered): 19,988,670 bp (48.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 72337 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 12 0.0 2 0 0 0 12 15 28 0.0 3 0 0 16 12 16 738 0.0 3 0 620 79 39 17 70552 0.0 3 65909 3868 644 131 18 242 0.0 3 16 134 12 80 19 147 0.0 3 4 2 70 71 20 383 0.0 3 1 0 0 382 21 3 0.0 3 1 0 0 2 22 2 0.0 3 1 0 1 23 2 0.0 3 1 0 0 1 24 1 0.0 3 0 0 1 26 1 0.0 3 1 31 1 0.0 3 0 0 0 1 32 3 0.0 3 0 1 0 2 40 1 0.0 3 0 0 0 1 43 19 0.0 3 0 0 0 19 45 3 0.0 3 0 0 0 3 46 5 0.0 3 0 0 0 5 48 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 69 3 0.0 3 0 0 0 3 71 2 0.0 3 0 0 0 2 73 1 0.0 3 0 0 0 1 79 3 0.0 3 0 0 0 3 86 4 0.0 3 0 0 0 4 87 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 1 121 1 0.0 3 0 0 0 1 123 3 0.0 3 0 0 0 3 128 5 0.0 3 0 0 0 5 129 3 0.0 3 0 0 0 3 131 1 0.0 3 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 7 0.0 3 0 0 0 7 136 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 5 0.0 3 0 0 1 4 146 2 0.0 3 0 0 0 2 147 3 0.0 3 0 0 0 3 148 3 0.0 3 0 0 0 3 149 2 0.0 3 0 0 0 2 151 3 0.0 3 0 0 0 3 152 4 0.0 3 0 0 0 4 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 173 2 0.0 3 0 0 0 2 174 2 0.0 3 0 0 0 2 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 1 190 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 0 2 192 2 0.0 3 0 0 0 2 194 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 217 21 0.0 3 0 0 2 19 222 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 238 4 0.0 3 0 0 0 4 240 1 0.0 3 0 0 0 1 241 2 0.0 3 0 0 0 2 245 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 280 3 0.0 3 0 0 0 3 281 12 0.0 3 0 0 0 12 282 6 0.0 3 0 0 1 5 283 3 0.0 3 0 0 0 3 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.52 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 72,223 Reads with adapters: 643 (0.9%) Reads written (passing filters): 72,223 (100.0%) Total basepairs processed: 19,988,670 bp Total written (filtered): 19,986,699 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 643 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.2% C: 61.9% G: 15.7% T: 4.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 619 1128.5 0 619 4 12 282.1 0 12 5 6 70.5 0 6 6 6 17.6 0 6