This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.06 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 150,699 (5.3%) Reads that were too short: 7,091 (0.3%) Reads written (passing filters): 143,608 (5.1%) Total basepairs processed: 853,219,115 bp Total written (filtered): 41,357,999 bp (4.8%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 150699 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1019 692.0 0 1019 7 63091 692.0 0 63091 8 72280 692.0 0 72280 9 53 692.0 0 53 10 13 692.0 0 13 11 16 692.0 0 16 12 8 692.0 0 8 13 8 692.0 0 8 14 15 692.0 0 15 15 13 692.0 0 13 16 15 692.0 0 15 17 9 692.0 0 9 18 27 692.0 0 27 19 21 692.0 0 21 20 28 692.0 0 28 21 14 692.0 0 14 22 13 692.0 0 13 23 12 692.0 0 12 24 9 692.0 0 9 25 7 692.0 0 7 26 13 692.0 0 13 27 15 692.0 0 15 28 13 692.0 0 13 29 17 692.0 0 17 30 11 692.0 0 11 31 7 692.0 0 7 32 18 692.0 0 18 33 9 692.0 0 9 34 20 692.0 0 20 35 22 692.0 0 22 36 73 692.0 0 73 37 76 692.0 0 76 38 174 692.0 0 174 39 9 692.0 0 9 40 15 692.0 0 15 41 10 692.0 0 10 42 18 692.0 0 18 43 42 692.0 0 42 44 61 692.0 0 61 45 162 692.0 0 162 46 10 692.0 0 10 47 18 692.0 0 18 48 17 692.0 0 17 49 30 692.0 0 30 50 24 692.0 0 24 51 17 692.0 0 17 52 17 692.0 0 17 53 21 692.0 0 21 54 13 692.0 0 13 55 22 692.0 0 22 56 32 692.0 0 32 57 47 692.0 0 47 58 27 692.0 0 27 59 35 692.0 0 35 60 9 692.0 0 9 61 22 692.0 0 22 62 24 692.0 0 24 63 22 692.0 0 22 64 17 692.0 0 17 65 13 692.0 0 13 66 18 692.0 0 18 67 10 692.0 0 10 68 13 692.0 0 13 69 19 692.0 0 19 70 24 692.0 0 24 71 71 692.0 0 71 72 6 692.0 0 6 73 31 692.0 0 31 74 12 692.0 0 12 75 13 692.0 0 13 76 17 692.0 0 17 77 25 692.0 0 25 78 24 692.0 0 24 79 42 692.0 0 42 80 16 692.0 0 16 81 10 692.0 0 10 82 21 692.0 0 21 83 13 692.0 0 13 84 20 692.0 0 20 85 17 692.0 0 17 86 19 692.0 0 19 87 44 692.0 0 44 88 35 692.0 0 35 89 40 692.0 0 40 90 26 692.0 0 26 91 27 692.0 0 27 92 18 692.0 0 18 93 29 692.0 0 29 94 49 692.0 0 49 95 32 692.0 0 32 96 35 692.0 0 35 97 40 692.0 0 40 98 27 692.0 0 27 99 21 692.0 0 21 100 9 692.0 0 9 101 16 692.0 0 16 102 10 692.0 0 10 103 19 692.0 0 19 104 25 692.0 0 25 105 21 692.0 0 21 106 18 692.0 0 18 107 24 692.0 0 24 108 20 692.0 0 20 109 21 692.0 0 21 110 14 692.0 0 14 111 11 692.0 0 11 112 30 692.0 0 30 113 26 692.0 0 26 114 16 692.0 0 16 115 30 692.0 0 30 116 13 692.0 0 13 117 21 692.0 0 21 118 27 692.0 0 27 119 21 692.0 0 21 120 37 692.0 0 37 121 36 692.0 0 36 122 38 692.0 0 38 123 16 692.0 0 16 124 13 692.0 0 13 125 25 692.0 0 25 126 46 692.0 0 46 127 27 692.0 0 27 128 68 692.0 0 68 129 53 692.0 0 53 130 43 692.0 0 43 131 45 692.0 0 45 132 37 692.0 0 37 133 135 692.0 0 135 134 166 692.0 0 166 135 845 692.0 0 845 136 180 692.0 0 180 137 303 692.0 0 303 138 32 692.0 0 32 139 30 692.0 0 30 140 80 692.0 0 80 141 111 692.0 0 111 142 82 692.0 0 82 143 53 692.0 0 53 144 31 692.0 0 31 145 26 692.0 0 26 146 35 692.0 0 35 147 53 692.0 0 53 148 83 692.0 0 83 149 199 692.0 0 199 150 99 692.0 0 99 151 39 692.0 0 39 152 21 692.0 0 21 153 58 692.0 0 58 154 27 692.0 0 27 155 31 692.0 0 31 156 29 692.0 0 29 157 20 692.0 0 20 158 14 692.0 0 14 159 20 692.0 0 20 160 26 692.0 0 26 161 21 692.0 0 21 162 20 692.0 0 20 163 24 692.0 0 24 164 28 692.0 0 28 165 27 692.0 0 27 166 28 692.0 0 28 167 32 692.0 0 32 168 17 692.0 0 17 169 20 692.0 0 20 170 21 692.0 0 21 171 22 692.0 0 22 172 20 692.0 0 20 173 35 692.0 0 35 174 33 692.0 0 33 175 40 692.0 0 40 176 20 692.0 0 20 177 22 692.0 0 22 178 20 692.0 0 20 179 23 692.0 0 23 180 40 692.0 0 40 181 34 692.0 0 34 182 44 692.0 0 44 183 19 692.0 0 19 184 28 692.0 0 28 185 29 692.0 0 29 186 34 692.0 0 34 187 27 692.0 0 27 188 26 692.0 0 26 189 25 692.0 0 25 190 29 692.0 0 29 191 33 692.0 0 33 192 20 692.0 0 20 193 34 692.0 0 34 194 39 692.0 0 39 195 62 692.0 0 62 196 23 692.0 0 23 197 24 692.0 0 24 198 20 692.0 0 20 199 22 692.0 0 22 200 25 692.0 0 25 201 37 692.0 0 37 202 64 692.0 0 64 203 42 692.0 0 42 204 19 692.0 0 19 205 33 692.0 0 33 206 46 692.0 0 46 207 57 692.0 0 57 208 56 692.0 0 56 209 126 692.0 0 126 210 49 692.0 0 49 211 39 692.0 0 39 212 96 692.0 0 96 213 50 692.0 0 50 214 50 692.0 0 50 215 77 692.0 0 77 216 73 692.0 0 73 217 28 692.0 0 28 218 25 692.0 0 25 219 36 692.0 0 36 220 59 692.0 0 59 221 52 692.0 0 52 222 38 692.0 0 38 223 34 692.0 0 34 224 28 692.0 0 28 225 30 692.0 0 30 226 32 692.0 0 32 227 50 692.0 0 50 228 48 692.0 0 48 229 44 692.0 0 44 230 84 692.0 0 84 231 37 692.0 0 37 232 48 692.0 0 48 233 68 692.0 0 68 234 58 692.0 0 58 235 101 692.0 0 101 236 48 692.0 0 48 237 38 692.0 0 38 238 39 692.0 0 39 239 24 692.0 0 24 240 39 692.0 0 39 241 29 692.0 0 29 242 38 692.0 0 38 243 45 692.0 0 45 244 49 692.0 0 49 245 36 692.0 0 36 246 26 692.0 0 26 247 30 692.0 0 30 248 41 692.0 0 41 249 27 692.0 0 27 250 23 692.0 0 23 251 29 692.0 0 29 252 54 692.0 0 54 253 42 692.0 0 42 254 29 692.0 0 29 255 33 692.0 0 33 256 36 692.0 0 36 257 33 692.0 0 33 258 49 692.0 0 49 259 37 692.0 0 37 260 39 692.0 0 39 261 47 692.0 0 47 262 38 692.0 0 38 263 50 692.0 0 50 264 40 692.0 0 40 265 35 692.0 0 35 266 34 692.0 0 34 267 56 692.0 0 56 268 52 692.0 0 52 269 257 692.0 0 257 270 114 692.0 0 114 271 256 692.0 0 256 272 57 692.0 0 57 273 66 692.0 0 66 274 80 692.0 0 80 275 65 692.0 0 65 276 66 692.0 0 66 277 49 692.0 0 49 278 59 692.0 0 59 279 54 692.0 0 54 280 82 692.0 0 82 281 148 692.0 0 148 282 130 692.0 0 130 283 65 692.0 0 65 284 44 692.0 0 44 285 50 692.0 0 50 286 58 692.0 0 58 287 48 692.0 0 48 288 77 692.0 0 77 289 81 692.0 0 81 290 59 692.0 0 59 291 57 692.0 0 57 292 80 692.0 0 80 293 113 692.0 0 113 294 335 692.0 0 335 295 344 692.0 0 344 296 538 692.0 0 538 297 232 692.0 0 232 298 127 692.0 0 127 299 48 692.0 0 48 300 57 692.0 0 57 301 153 692.0 0 153 Finished in 7.13 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 143,608 Reads with adapters: 75,335 (52.5%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 75,316 (52.4%) Total basepairs processed: 41,357,999 bp Total written (filtered): 20,527,813 bp (49.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 75335 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 17 0.0 3 0 0 16 1 19 829 0.0 3 0 805 20 3 1 20 73897 0.0 4 72624 1235 33 3 2 21 154 0.0 4 29 120 4 1 22 6 0.0 4 0 0 5 0 1 23 72 0.0 4 1 0 0 60 11 24 3 0.0 4 1 0 0 0 2 25 1 0.0 4 1 26 1 0.0 4 0 0 0 1 27 2 0.0 4 2 47 1 0.0 4 1 91 3 0.0 4 0 0 0 0 3 98 6 0.0 4 0 0 0 0 6 101 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 305 0.0 4 0 0 0 0 305 128 5 0.0 4 0 0 0 0 5 129 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 136 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 167 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 257 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 1 285 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 294 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.66 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 75,316 Reads with adapters: 7,463 (9.9%) Reads written (passing filters): 75,316 (100.0%) Total basepairs processed: 20,527,813 bp Total written (filtered): 20,444,455 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7463 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.5% C: 3.0% G: 93.8% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 29 1176.8 0 29 4 10 294.2 0 10 5 3 73.6 0 3 6 1 18.4 0 1 9 31 0.3 0 1 30 10 1006 0.1 1 44 962 11 6345 0.0 1 43 6302 12 13 0.0 1 1 12 13 8 0.0 1 0 8 14 3 0.0 1 0 3 151 1 0.0 1 0 1 170 1 0.0 1 1 171 1 0.0 1 1 187 1 0.0 1 0 1 190 1 0.0 1 1 191 1 0.0 1 0 1 209 6 0.0 1 1 5 230 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.