This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGCGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.44 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 137,272 (4.8%) Reads that were too short: 9,837 (0.3%) Reads written (passing filters): 127,435 (4.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 36,080,415 bp (4.2%) === Adapter 1 === Sequence: CGCGAT; Type: regular 5'; Length: 6; Trimmed: 137272 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3668 692.0 0 3668 7 63339 692.0 0 63339 8 671 692.0 0 671 9 48240 692.0 0 48240 10 30 692.0 0 30 11 22 692.0 0 22 12 26 692.0 0 26 13 34 692.0 0 34 14 19 692.0 0 19 15 19 692.0 0 19 16 26 692.0 0 26 17 22 692.0 0 22 18 13 692.0 0 13 19 22 692.0 0 22 20 19 692.0 0 19 21 21 692.0 0 21 22 20 692.0 0 20 23 25 692.0 0 25 24 15 692.0 0 15 25 16 692.0 0 16 26 32 692.0 0 32 27 23 692.0 0 23 28 26 692.0 0 26 29 26 692.0 0 26 30 23 692.0 0 23 31 17 692.0 0 17 32 25 692.0 0 25 33 13 692.0 0 13 34 11 692.0 0 11 35 20 692.0 0 20 36 29 692.0 0 29 37 28 692.0 0 28 38 39 692.0 0 39 39 23 692.0 0 23 40 26 692.0 0 26 41 28 692.0 0 28 42 143 692.0 0 143 43 85 692.0 0 85 44 201 692.0 0 201 45 23 692.0 0 23 46 13 692.0 0 13 47 21 692.0 0 21 48 18 692.0 0 18 49 20 692.0 0 20 50 18 692.0 0 18 51 17 692.0 0 17 52 24 692.0 0 24 53 32 692.0 0 32 54 33 692.0 0 33 55 23 692.0 0 23 56 33 692.0 0 33 57 15 692.0 0 15 58 36 692.0 0 36 59 20 692.0 0 20 60 17 692.0 0 17 61 16 692.0 0 16 62 20 692.0 0 20 63 18 692.0 0 18 64 19 692.0 0 19 65 30 692.0 0 30 66 37 692.0 0 37 67 46 692.0 0 46 68 15 692.0 0 15 69 28 692.0 0 28 70 24 692.0 0 24 71 22 692.0 0 22 72 20 692.0 0 20 73 21 692.0 0 21 74 28 692.0 0 28 75 48 692.0 0 48 76 24 692.0 0 24 77 49 692.0 0 49 78 12 692.0 0 12 79 29 692.0 0 29 80 19 692.0 0 19 81 52 692.0 0 52 82 14 692.0 0 14 83 24 692.0 0 24 84 21 692.0 0 21 85 31 692.0 0 31 86 26 692.0 0 26 87 41 692.0 0 41 88 32 692.0 0 32 89 33 692.0 0 33 90 44 692.0 0 44 91 47 692.0 0 47 92 391 692.0 0 391 93 375 692.0 0 375 94 667 692.0 0 667 95 31 692.0 0 31 96 20 692.0 0 20 97 26 692.0 0 26 98 26 692.0 0 26 99 34 692.0 0 34 100 24 692.0 0 24 101 44 692.0 0 44 102 28 692.0 0 28 103 21 692.0 0 21 104 25 692.0 0 25 105 27 692.0 0 27 106 31 692.0 0 31 107 28 692.0 0 28 108 41 692.0 0 41 109 38 692.0 0 38 110 49 692.0 0 49 111 29 692.0 0 29 112 44 692.0 0 44 113 41 692.0 0 41 114 32 692.0 0 32 115 39 692.0 0 39 116 39 692.0 0 39 117 42 692.0 0 42 118 56 692.0 0 56 119 48 692.0 0 48 120 59 692.0 0 59 121 61 692.0 0 61 122 61 692.0 0 61 123 1731 692.0 0 1731 124 297 692.0 0 297 125 239 692.0 0 239 126 24 692.0 0 24 127 27 692.0 0 27 128 89 692.0 0 89 129 203 692.0 0 203 130 417 692.0 0 417 131 61 692.0 0 61 132 245 692.0 0 245 133 34 692.0 0 34 134 24 692.0 0 24 135 27 692.0 0 27 136 27 692.0 0 27 137 28 692.0 0 28 138 23 692.0 0 23 139 25 692.0 0 25 140 25 692.0 0 25 141 17 692.0 0 17 142 21 692.0 0 21 143 29 692.0 0 29 144 22 692.0 0 22 145 47 692.0 0 47 146 25 692.0 0 25 147 95 692.0 0 95 148 27 692.0 0 27 149 26 692.0 0 26 150 23 692.0 0 23 151 22 692.0 0 22 152 36 692.0 0 36 153 24 692.0 0 24 154 26 692.0 0 26 155 28 692.0 0 28 156 22 692.0 0 22 157 26 692.0 0 26 158 65 692.0 0 65 159 16 692.0 0 16 160 20 692.0 0 20 161 20 692.0 0 20 162 31 692.0 0 31 163 23 692.0 0 23 164 34 692.0 0 34 165 28 692.0 0 28 166 16 692.0 0 16 167 49 692.0 0 49 168 27 692.0 0 27 169 37 692.0 0 37 170 24 692.0 0 24 171 23 692.0 0 23 172 25 692.0 0 25 173 41 692.0 0 41 174 52 692.0 0 52 175 51 692.0 0 51 176 35 692.0 0 35 177 43 692.0 0 43 178 113 692.0 0 113 179 103 692.0 0 103 180 129 692.0 0 129 181 59 692.0 0 59 182 31 692.0 0 31 183 30 692.0 0 30 184 70 692.0 0 70 185 42 692.0 0 42 186 104 692.0 0 104 187 24 692.0 0 24 188 42 692.0 0 42 189 35 692.0 0 35 190 28 692.0 0 28 191 22 692.0 0 22 192 44 692.0 0 44 193 32 692.0 0 32 194 45 692.0 0 45 195 91 692.0 0 91 196 64 692.0 0 64 197 38 692.0 0 38 198 189 692.0 0 189 199 136 692.0 0 136 200 336 692.0 0 336 201 91 692.0 0 91 202 62 692.0 0 62 203 39 692.0 0 39 204 53 692.0 0 53 205 43 692.0 0 43 206 39 692.0 0 39 207 44 692.0 0 44 208 28 692.0 0 28 209 29 692.0 0 29 210 25 692.0 0 25 211 41 692.0 0 41 212 47 692.0 0 47 213 21 692.0 0 21 214 70 692.0 0 70 215 32 692.0 0 32 216 43 692.0 0 43 217 51 692.0 0 51 218 50 692.0 0 50 219 92 692.0 0 92 220 85 692.0 0 85 221 162 692.0 0 162 222 515 692.0 0 515 223 182 692.0 0 182 224 469 692.0 0 469 225 57 692.0 0 57 226 37 692.0 0 37 227 27 692.0 0 27 228 57 692.0 0 57 229 49 692.0 0 49 230 34 692.0 0 34 231 36 692.0 0 36 232 24 692.0 0 24 233 38 692.0 0 38 234 43 692.0 0 43 235 46 692.0 0 46 236 39 692.0 0 39 237 55 692.0 0 55 238 192 692.0 0 192 239 265 692.0 0 265 240 1838 692.0 0 1838 241 762 692.0 0 762 242 947 692.0 0 947 243 22 692.0 0 22 244 45 692.0 0 45 245 34 692.0 0 34 246 33 692.0 0 33 247 55 692.0 0 55 248 61 692.0 0 61 249 51 692.0 0 51 250 66 692.0 0 66 251 96 692.0 0 96 252 149 692.0 0 149 253 36 692.0 0 36 254 24 692.0 0 24 255 19 692.0 0 19 256 34 692.0 0 34 257 40 692.0 0 40 258 43 692.0 0 43 259 32 692.0 0 32 260 38 692.0 0 38 261 62 692.0 0 62 262 55 692.0 0 55 263 237 692.0 0 237 264 26 692.0 0 26 265 30 692.0 0 30 266 28 692.0 0 28 267 38 692.0 0 38 268 43 692.0 0 43 269 41 692.0 0 41 270 30 692.0 0 30 271 33 692.0 0 33 272 41 692.0 0 41 273 27 692.0 0 27 274 26 692.0 0 26 275 33 692.0 0 33 276 34 692.0 0 34 277 39 692.0 0 39 278 54 692.0 0 54 279 38 692.0 0 38 280 38 692.0 0 38 281 31 692.0 0 31 282 29 692.0 0 29 283 31 692.0 0 31 284 37 692.0 0 37 285 48 692.0 0 48 286 46 692.0 0 46 287 70 692.0 0 70 288 103 692.0 0 103 289 81 692.0 0 81 290 158 692.0 0 158 291 121 692.0 0 121 292 58 692.0 0 58 293 39 692.0 0 39 294 40 692.0 0 40 295 32 692.0 0 32 296 56 692.0 0 56 297 36 692.0 0 36 298 27 692.0 0 27 299 48 692.0 0 48 300 72 692.0 0 72 301 75 692.0 0 75 Finished in 6.36 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 127,435 Reads with adapters: 66,425 (52.1%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 66,411 (52.1%) Total basepairs processed: 36,080,415 bp Total written (filtered): 18,193,421 bp (50.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 66425 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 1 3 18 24 0.0 3 0 0 22 2 19 731 0.0 3 0 710 18 0 3 20 65447 0.0 4 64370 1053 22 2 21 111 0.0 4 16 94 1 22 5 0.0 4 1 0 4 23 31 0.0 4 1 0 0 28 2 24 3 0.0 4 0 0 1 0 2 30 1 0.0 4 1 86 1 0.0 4 0 0 0 0 1 98 1 0.0 4 1 101 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 119 1 0.0 4 1 124 1 0.0 4 0 1 126 1 0.0 4 0 0 0 0 1 127 40 0.0 4 0 0 0 0 40 130 2 0.0 4 0 0 0 0 2 135 1 0.0 4 1 136 2 0.0 4 2 139 1 0.0 4 0 1 146 1 0.0 4 1 150 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 194 6 0.0 4 0 0 0 1 5 204 1 0.0 4 1 207 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 262 1 0.0 4 1 263 1 0.0 4 1 266 1 0.0 4 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.25 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 66,411 Reads with adapters: 536 (0.8%) Reads written (passing filters): 66,411 (100.0%) Total basepairs processed: 18,193,421 bp Total written (filtered): 18,178,334 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 536 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 17.4% C: 5.8% G: 72.6% T: 4.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 75 1037.7 0 75 4 2 259.4 0 2 5 1 64.9 0 1 9 3 0.3 0 0 3 10 1 0.1 1 0 1 11 199 0.0 1 1 198 12 85 0.0 1 42 43 13 2 0.0 1 2 15 106 0.0 1 83 23 16 9 0.0 1 5 4 20 2 0.0 1 2 21 2 0.0 1 2 29 1 0.0 1 1 33 2 0.0 1 1 1 152 2 0.0 1 0 2 210 34 0.0 1 23 11 211 2 0.0 1 2 225 8 0.0 1 8