This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.41 s (28 us/read; 2.17 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 246,438 (8.7%) Reads that were too short: 36,536 (1.3%) Reads written (passing filters): 209,902 (7.4%) Total basepairs processed: 853,219,115 bp Total written (filtered): 56,134,732 bp (6.6%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 246438 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2490 692.0 0 2490 7 67239 692.0 0 67239 8 2352 692.0 0 2352 9 42371 692.0 0 42371 10 1563 692.0 0 1563 11 41963 692.0 0 41963 12 107 692.0 0 107 13 40 692.0 0 40 14 70 692.0 0 70 15 58 692.0 0 58 16 45 692.0 0 45 17 36 692.0 0 36 18 58 692.0 0 58 19 44 692.0 0 44 20 58 692.0 0 58 21 38 692.0 0 38 22 35 692.0 0 35 23 49 692.0 0 49 24 62 692.0 0 62 25 56 692.0 0 56 26 67 692.0 0 67 27 116 692.0 0 116 28 108 692.0 0 108 29 119 692.0 0 119 30 614 692.0 0 614 31 54 692.0 0 54 32 67 692.0 0 67 33 47 692.0 0 47 34 47 692.0 0 47 35 43 692.0 0 43 36 72 692.0 0 72 37 55 692.0 0 55 38 61 692.0 0 61 39 62 692.0 0 62 40 54 692.0 0 54 41 148 692.0 0 148 42 69 692.0 0 69 43 57 692.0 0 57 44 78 692.0 0 78 45 63 692.0 0 63 46 77 692.0 0 77 47 62 692.0 0 62 48 81 692.0 0 81 49 79 692.0 0 79 50 269 692.0 0 269 51 40 692.0 0 40 52 65 692.0 0 65 53 49 692.0 0 49 54 56 692.0 0 56 55 89 692.0 0 89 56 92 692.0 0 92 57 123 692.0 0 123 58 46 692.0 0 46 59 63 692.0 0 63 60 79 692.0 0 79 61 64 692.0 0 64 62 79 692.0 0 79 63 65 692.0 0 65 64 81 692.0 0 81 65 74 692.0 0 74 66 76 692.0 0 76 67 113 692.0 0 113 68 72 692.0 0 72 69 98 692.0 0 98 70 281 692.0 0 281 71 144 692.0 0 144 72 800 692.0 0 800 73 76 692.0 0 76 74 80 692.0 0 80 75 97 692.0 0 97 76 91 692.0 0 91 77 104 692.0 0 104 78 123 692.0 0 123 79 495 692.0 0 495 80 1698 692.0 0 1698 81 198 692.0 0 198 82 135 692.0 0 135 83 1349 692.0 0 1349 84 1237 692.0 0 1237 85 3753 692.0 0 3753 86 81 692.0 0 81 87 44 692.0 0 44 88 61 692.0 0 61 89 66 692.0 0 66 90 97 692.0 0 97 91 70 692.0 0 70 92 51 692.0 0 51 93 41 692.0 0 41 94 41 692.0 0 41 95 69 692.0 0 69 96 64 692.0 0 64 97 113 692.0 0 113 98 123 692.0 0 123 99 298 692.0 0 298 100 300 692.0 0 300 101 109 692.0 0 109 102 142 692.0 0 142 103 158 692.0 0 158 104 110 692.0 0 110 105 139 692.0 0 139 106 464 692.0 0 464 107 182 692.0 0 182 108 189 692.0 0 189 109 157 692.0 0 157 110 220 692.0 0 220 111 281 692.0 0 281 112 365 692.0 0 365 113 4503 692.0 0 4503 114 3043 692.0 0 3043 115 13531 692.0 0 13531 116 132 692.0 0 132 117 53 692.0 0 53 118 46 692.0 0 46 119 56 692.0 0 56 120 45 692.0 0 45 121 54 692.0 0 54 122 57 692.0 0 57 123 66 692.0 0 66 124 82 692.0 0 82 125 72 692.0 0 72 126 185 692.0 0 185 127 83 692.0 0 83 128 91 692.0 0 91 129 72 692.0 0 72 130 102 692.0 0 102 131 145 692.0 0 145 132 56 692.0 0 56 133 110 692.0 0 110 134 91 692.0 0 91 135 174 692.0 0 174 136 205 692.0 0 205 137 82 692.0 0 82 138 135 692.0 0 135 139 168 692.0 0 168 140 141 692.0 0 141 141 140 692.0 0 140 142 170 692.0 0 170 143 100 692.0 0 100 144 75 692.0 0 75 145 87 692.0 0 87 146 188 692.0 0 188 147 78 692.0 0 78 148 105 692.0 0 105 149 97 692.0 0 97 150 350 692.0 0 350 151 65 692.0 0 65 152 68 692.0 0 68 153 91 692.0 0 91 154 1035 692.0 0 1035 155 655 692.0 0 655 156 532 692.0 0 532 157 807 692.0 0 807 158 156 692.0 0 156 159 74 692.0 0 74 160 77 692.0 0 77 161 66 692.0 0 66 162 58 692.0 0 58 163 180 692.0 0 180 164 65 692.0 0 65 165 122 692.0 0 122 166 56 692.0 0 56 167 387 692.0 0 387 168 113 692.0 0 113 169 79 692.0 0 79 170 184 692.0 0 184 171 67 692.0 0 67 172 58 692.0 0 58 173 87 692.0 0 87 174 48 692.0 0 48 175 48 692.0 0 48 176 54 692.0 0 54 177 53 692.0 0 53 178 55 692.0 0 55 179 287 692.0 0 287 180 41 692.0 0 41 181 44 692.0 0 44 182 80 692.0 0 80 183 51 692.0 0 51 184 120 692.0 0 120 185 106 692.0 0 106 186 102 692.0 0 102 187 95 692.0 0 95 188 66 692.0 0 66 189 134 692.0 0 134 190 57 692.0 0 57 191 61 692.0 0 61 192 70 692.0 0 70 193 68 692.0 0 68 194 70 692.0 0 70 195 66 692.0 0 66 196 69 692.0 0 69 197 88 692.0 0 88 198 61 692.0 0 61 199 65 692.0 0 65 200 253 692.0 0 253 201 115 692.0 0 115 202 152 692.0 0 152 203 56 692.0 0 56 204 76 692.0 0 76 205 49 692.0 0 49 206 58 692.0 0 58 207 68 692.0 0 68 208 59 692.0 0 59 209 71 692.0 0 71 210 84 692.0 0 84 211 375 692.0 0 375 212 353 692.0 0 353 213 1127 692.0 0 1127 214 133 692.0 0 133 215 103 692.0 0 103 216 90 692.0 0 90 217 78 692.0 0 78 218 74 692.0 0 74 219 51 692.0 0 51 220 60 692.0 0 60 221 84 692.0 0 84 222 75 692.0 0 75 223 83 692.0 0 83 224 60 692.0 0 60 225 77 692.0 0 77 226 91 692.0 0 91 227 135 692.0 0 135 228 1229 692.0 0 1229 229 406 692.0 0 406 230 1204 692.0 0 1204 231 119 692.0 0 119 232 226 692.0 0 226 233 97 692.0 0 97 234 132 692.0 0 132 235 187 692.0 0 187 236 276 692.0 0 276 237 172 692.0 0 172 238 143 692.0 0 143 239 64 692.0 0 64 240 72 692.0 0 72 241 75 692.0 0 75 242 52 692.0 0 52 243 87 692.0 0 87 244 95 692.0 0 95 245 74 692.0 0 74 246 138 692.0 0 138 247 196 692.0 0 196 248 362 692.0 0 362 249 173 692.0 0 173 250 334 692.0 0 334 251 87 692.0 0 87 252 104 692.0 0 104 253 95 692.0 0 95 254 190 692.0 0 190 255 165 692.0 0 165 256 221 692.0 0 221 257 330 692.0 0 330 258 169 692.0 0 169 259 69 692.0 0 69 260 81 692.0 0 81 261 64 692.0 0 64 262 83 692.0 0 83 263 62 692.0 0 62 264 100 692.0 0 100 265 97 692.0 0 97 266 59 692.0 0 59 267 89 692.0 0 89 268 195 692.0 0 195 269 697 692.0 0 697 270 607 692.0 0 607 271 271 692.0 0 271 272 129 692.0 0 129 273 83 692.0 0 83 274 240 692.0 0 240 275 80 692.0 0 80 276 79 692.0 0 79 277 93 692.0 0 93 278 118 692.0 0 118 279 111 692.0 0 111 280 481 692.0 0 481 281 200 692.0 0 200 282 232 692.0 0 232 283 180 692.0 0 180 284 153 692.0 0 153 285 238 692.0 0 238 286 105 692.0 0 105 287 109 692.0 0 109 288 192 692.0 0 192 289 174 692.0 0 174 290 187 692.0 0 187 291 138 692.0 0 138 292 183 692.0 0 183 293 251 692.0 0 251 294 486 692.0 0 486 295 1257 692.0 0 1257 296 5834 692.0 0 5834 297 2537 692.0 0 2537 298 5675 692.0 0 5675 299 1261 692.0 0 1261 300 2092 692.0 0 2092 301 168 692.0 0 168 Finished in 10.10 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 209,902 Reads with adapters: 67,316 (32.1%) Reads that were too short: 297 (0.1%) Reads written (passing filters): 67,019 (31.9%) Total basepairs processed: 56,134,732 bp Total written (filtered): 18,516,402 bp (33.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 67316 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 40 0.0 3 0 0 25 15 16 427 0.0 3 0 337 66 24 17 65170 0.0 3 61460 3040 529 141 18 362 0.0 3 16 114 8 224 19 84 0.0 3 22 3 34 25 20 614 0.0 3 1 0 1 612 21 3 0.0 3 0 0 0 3 23 19 0.0 3 1 0 0 18 27 1 0.0 3 1 29 1 0.0 3 0 0 0 1 30 4 0.0 3 0 0 0 4 31 1 0.0 3 1 32 94 0.0 3 0 0 1 93 34 5 0.0 3 1 0 0 4 35 16 0.0 3 0 0 0 16 37 2 0.0 3 2 39 2 0.0 3 0 0 1 1 41 1 0.0 3 0 0 0 1 43 3 0.0 3 0 0 0 3 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 47 3 0.0 3 0 0 0 3 48 1 0.0 3 0 1 49 4 0.0 3 0 0 0 4 50 5 0.0 3 1 0 1 3 51 8 0.0 3 0 0 1 7 52 1 0.0 3 0 0 0 1 53 25 0.0 3 0 0 0 25 54 1 0.0 3 0 0 0 1 56 9 0.0 3 0 0 1 8 58 7 0.0 3 0 0 0 7 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 62 2 0.0 3 0 0 0 2 64 2 0.0 3 0 0 1 1 65 3 0.0 3 0 0 0 3 67 2 0.0 3 0 0 0 2 68 1 0.0 3 1 70 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 1 73 21 0.0 3 0 0 18 3 76 3 0.0 3 0 0 0 3 77 15 0.0 3 0 0 0 15 78 3 0.0 3 0 0 0 3 79 4 0.0 3 0 0 1 3 80 2 0.0 3 1 0 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 84 4 0.0 3 0 0 0 4 85 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 91 3 0.0 3 0 0 0 3 94 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 96 4 0.0 3 0 0 0 4 98 2 0.0 3 0 0 1 1 99 1 0.0 3 0 0 0 1 104 14 0.0 3 0 0 1 13 105 2 0.0 3 0 0 2 106 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 113 2 0.0 3 0 0 0 2 115 3 0.0 3 0 0 0 3 116 1 0.0 3 0 0 0 1 120 2 0.0 3 0 1 0 1 121 2 0.0 3 2 122 11 0.0 3 0 0 1 10 124 1 0.0 3 0 0 0 1 125 10 0.0 3 0 0 0 10 126 2 0.0 3 0 0 0 2 129 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 1 133 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 144 6 0.0 3 0 0 0 6 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 152 2 0.0 3 0 0 1 1 153 10 0.0 3 0 0 0 10 154 25 0.0 3 0 0 1 24 155 2 0.0 3 0 0 0 2 156 7 0.0 3 0 0 0 7 161 1 0.0 3 0 0 0 1 165 7 0.0 3 0 0 0 7 166 4 0.0 3 0 0 0 4 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 171 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 1 176 1 0.0 3 0 0 0 1 178 4 0.0 3 0 0 1 3 179 3 0.0 3 0 0 0 3 180 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 184 2 0.0 3 0 0 0 2 185 2 0.0 3 0 0 0 2 187 1 0.0 3 0 1 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 190 4 0.0 3 0 0 0 4 191 2 0.0 3 0 0 0 2 192 14 0.0 3 0 0 0 14 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 197 1 0.0 3 1 198 1 0.0 3 0 0 0 1 200 14 0.0 3 0 0 0 14 201 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 207 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 2 0.0 3 0 0 0 2 215 7 0.0 3 0 0 1 6 218 1 0.0 3 0 0 0 1 219 2 0.0 3 0 0 0 2 224 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 236 17 0.0 3 0 0 1 16 237 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 239 1 0.0 3 0 0 0 1 248 1 0.0 3 1 249 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 264 2 0.0 3 0 0 0 2 268 2 0.0 3 0 0 0 2 269 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 1 277 2 0.0 3 0 0 0 2 278 1 0.0 3 0 0 0 1 279 16 0.0 3 0 0 0 16 280 4 0.0 3 0 0 0 4 281 14 0.0 3 0 0 0 14 282 6 0.0 3 0 0 1 5 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.36 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 67,019 Reads with adapters: 325 (0.5%) Reads written (passing filters): 67,019 (100.0%) Total basepairs processed: 18,516,402 bp Total written (filtered): 18,515,393 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 325 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.4% C: 42.5% G: 25.2% T: 4.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 304 1047.2 0 304 4 10 261.8 0 10 5 9 65.4 0 9 6 2 16.4 0 2