This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.11 s (27 us/read; 2.21 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 249,618 (8.8%) Reads that were too short: 27,635 (1.0%) Reads written (passing filters): 221,983 (7.8%) Total basepairs processed: 853,219,115 bp Total written (filtered): 59,398,197 bp (7.0%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 249618 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2516 692.0 0 2516 7 53403 692.0 0 53403 8 2096 692.0 0 2096 9 59019 692.0 0 59019 10 1245 692.0 0 1245 11 50214 692.0 0 50214 12 99 692.0 0 99 13 36 692.0 0 36 14 37 692.0 0 37 15 38 692.0 0 38 16 36 692.0 0 36 17 42 692.0 0 42 18 40 692.0 0 40 19 40 692.0 0 40 20 36 692.0 0 36 21 48 692.0 0 48 22 53 692.0 0 53 23 53 692.0 0 53 24 51 692.0 0 51 25 47 692.0 0 47 26 50 692.0 0 50 27 40 692.0 0 40 28 68 692.0 0 68 29 77 692.0 0 77 30 634 692.0 0 634 31 45 692.0 0 45 32 49 692.0 0 49 33 49 692.0 0 49 34 52 692.0 0 52 35 55 692.0 0 55 36 54 692.0 0 54 37 51 692.0 0 51 38 69 692.0 0 69 39 79 692.0 0 79 40 57 692.0 0 57 41 183 692.0 0 183 42 90 692.0 0 90 43 39 692.0 0 39 44 63 692.0 0 63 45 58 692.0 0 58 46 68 692.0 0 68 47 59 692.0 0 59 48 72 692.0 0 72 49 74 692.0 0 74 50 283 692.0 0 283 51 54 692.0 0 54 52 57 692.0 0 57 53 70 692.0 0 70 54 63 692.0 0 63 55 95 692.0 0 95 56 104 692.0 0 104 57 62 692.0 0 62 58 75 692.0 0 75 59 62 692.0 0 62 60 80 692.0 0 80 61 69 692.0 0 69 62 93 692.0 0 93 63 79 692.0 0 79 64 69 692.0 0 69 65 77 692.0 0 77 66 81 692.0 0 81 67 122 692.0 0 122 68 81 692.0 0 81 69 89 692.0 0 89 70 284 692.0 0 284 71 138 692.0 0 138 72 752 692.0 0 752 73 83 692.0 0 83 74 70 692.0 0 70 75 81 692.0 0 81 76 64 692.0 0 64 77 87 692.0 0 87 78 107 692.0 0 107 79 363 692.0 0 363 80 1823 692.0 0 1823 81 168 692.0 0 168 82 137 692.0 0 137 83 1438 692.0 0 1438 84 1358 692.0 0 1358 85 4088 692.0 0 4088 86 81 692.0 0 81 87 58 692.0 0 58 88 50 692.0 0 50 89 70 692.0 0 70 90 72 692.0 0 72 91 69 692.0 0 69 92 58 692.0 0 58 93 55 692.0 0 55 94 44 692.0 0 44 95 64 692.0 0 64 96 82 692.0 0 82 97 106 692.0 0 106 98 121 692.0 0 121 99 315 692.0 0 315 100 329 692.0 0 329 101 137 692.0 0 137 102 167 692.0 0 167 103 180 692.0 0 180 104 85 692.0 0 85 105 156 692.0 0 156 106 509 692.0 0 509 107 158 692.0 0 158 108 204 692.0 0 204 109 127 692.0 0 127 110 216 692.0 0 216 111 259 692.0 0 259 112 329 692.0 0 329 113 4476 692.0 0 4476 114 2731 692.0 0 2731 115 14494 692.0 0 14494 116 114 692.0 0 114 117 52 692.0 0 52 118 58 692.0 0 58 119 50 692.0 0 50 120 72 692.0 0 72 121 50 692.0 0 50 122 40 692.0 0 40 123 70 692.0 0 70 124 65 692.0 0 65 125 63 692.0 0 63 126 153 692.0 0 153 127 65 692.0 0 65 128 88 692.0 0 88 129 59 692.0 0 59 130 93 692.0 0 93 131 140 692.0 0 140 132 50 692.0 0 50 133 81 692.0 0 81 134 61 692.0 0 61 135 136 692.0 0 136 136 117 692.0 0 117 137 57 692.0 0 57 138 61 692.0 0 61 139 100 692.0 0 100 140 112 692.0 0 112 141 119 692.0 0 119 142 173 692.0 0 173 143 75 692.0 0 75 144 75 692.0 0 75 145 81 692.0 0 81 146 228 692.0 0 228 147 81 692.0 0 81 148 102 692.0 0 102 149 67 692.0 0 67 150 386 692.0 0 386 151 78 692.0 0 78 152 73 692.0 0 73 153 86 692.0 0 86 154 1202 692.0 0 1202 155 787 692.0 0 787 156 700 692.0 0 700 157 1069 692.0 0 1069 158 186 692.0 0 186 159 88 692.0 0 88 160 88 692.0 0 88 161 53 692.0 0 53 162 43 692.0 0 43 163 202 692.0 0 202 164 57 692.0 0 57 165 119 692.0 0 119 166 88 692.0 0 88 167 464 692.0 0 464 168 77 692.0 0 77 169 72 692.0 0 72 170 277 692.0 0 277 171 65 692.0 0 65 172 68 692.0 0 68 173 98 692.0 0 98 174 64 692.0 0 64 175 70 692.0 0 70 176 58 692.0 0 58 177 67 692.0 0 67 178 79 692.0 0 79 179 421 692.0 0 421 180 56 692.0 0 56 181 67 692.0 0 67 182 92 692.0 0 92 183 67 692.0 0 67 184 129 692.0 0 129 185 106 692.0 0 106 186 94 692.0 0 94 187 91 692.0 0 91 188 43 692.0 0 43 189 169 692.0 0 169 190 64 692.0 0 64 191 66 692.0 0 66 192 55 692.0 0 55 193 52 692.0 0 52 194 55 692.0 0 55 195 60 692.0 0 60 196 69 692.0 0 69 197 79 692.0 0 79 198 64 692.0 0 64 199 68 692.0 0 68 200 276 692.0 0 276 201 76 692.0 0 76 202 137 692.0 0 137 203 60 692.0 0 60 204 53 692.0 0 53 205 39 692.0 0 39 206 60 692.0 0 60 207 47 692.0 0 47 208 66 692.0 0 66 209 51 692.0 0 51 210 58 692.0 0 58 211 111 692.0 0 111 212 98 692.0 0 98 213 269 692.0 0 269 214 104 692.0 0 104 215 69 692.0 0 69 216 92 692.0 0 92 217 56 692.0 0 56 218 62 692.0 0 62 219 45 692.0 0 45 220 62 692.0 0 62 221 72 692.0 0 72 222 67 692.0 0 67 223 72 692.0 0 72 224 54 692.0 0 54 225 73 692.0 0 73 226 62 692.0 0 62 227 65 692.0 0 65 228 354 692.0 0 354 229 151 692.0 0 151 230 303 692.0 0 303 231 88 692.0 0 88 232 143 692.0 0 143 233 63 692.0 0 63 234 76 692.0 0 76 235 75 692.0 0 75 236 151 692.0 0 151 237 92 692.0 0 92 238 63 692.0 0 63 239 78 692.0 0 78 240 56 692.0 0 56 241 59 692.0 0 59 242 47 692.0 0 47 243 68 692.0 0 68 244 60 692.0 0 60 245 59 692.0 0 59 246 74 692.0 0 74 247 82 692.0 0 82 248 208 692.0 0 208 249 113 692.0 0 113 250 421 692.0 0 421 251 66 692.0 0 66 252 77 692.0 0 77 253 75 692.0 0 75 254 75 692.0 0 75 255 69 692.0 0 69 256 92 692.0 0 92 257 109 692.0 0 109 258 94 692.0 0 94 259 55 692.0 0 55 260 81 692.0 0 81 261 41 692.0 0 41 262 64 692.0 0 64 263 56 692.0 0 56 264 71 692.0 0 71 265 60 692.0 0 60 266 54 692.0 0 54 267 118 692.0 0 118 268 108 692.0 0 108 269 261 692.0 0 261 270 209 692.0 0 209 271 124 692.0 0 124 272 78 692.0 0 78 273 60 692.0 0 60 274 94 692.0 0 94 275 32 692.0 0 32 276 77 692.0 0 77 277 59 692.0 0 59 278 90 692.0 0 90 279 76 692.0 0 76 280 143 692.0 0 143 281 151 692.0 0 151 282 120 692.0 0 120 283 139 692.0 0 139 284 151 692.0 0 151 285 229 692.0 0 229 286 88 692.0 0 88 287 102 692.0 0 102 288 150 692.0 0 150 289 142 692.0 0 142 290 108 692.0 0 108 291 140 692.0 0 140 292 129 692.0 0 129 293 160 692.0 0 160 294 302 692.0 0 302 295 839 692.0 0 839 296 3666 692.0 0 3666 297 1519 692.0 0 1519 298 6737 692.0 0 6737 299 1794 692.0 0 1794 300 3314 692.0 0 3314 301 99 692.0 0 99 Finished in 11.16 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 221,983 Reads with adapters: 62,618 (28.2%) Reads that were too short: 49 (0.0%) Reads written (passing filters): 62,569 (28.2%) Total basepairs processed: 59,398,197 bp Total written (filtered): 17,024,380 bp (28.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 62618 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 2 2 18 20 0.0 3 0 0 20 19 723 0.0 3 0 707 13 2 1 20 61619 0.0 4 60458 1119 39 3 21 125 0.0 4 40 80 4 0 1 22 8 0.0 4 0 1 7 23 50 0.0 4 1 0 2 44 3 24 1 0.0 4 0 0 0 1 25 1 0.0 4 1 26 1 0.0 4 1 27 1 0.0 4 0 0 0 0 1 39 1 0.0 4 1 41 1 0.0 4 1 50 1 0.0 4 1 52 1 0.0 4 0 1 87 2 0.0 4 0 1 0 0 1 88 1 0.0 4 0 1 91 2 0.0 4 0 2 92 3 0.0 4 2 1 93 1 0.0 4 1 95 1 0.0 4 1 102 1 0.0 4 1 113 2 0.0 4 1 1 121 9 0.0 4 0 0 0 0 9 122 2 0.0 4 1 0 0 0 1 123 2 0.0 4 2 126 3 0.0 4 2 0 0 0 1 127 5 0.0 4 0 0 0 0 5 132 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 1 144 1 0.0 4 0 0 1 151 7 0.0 4 0 0 0 1 6 153 2 0.0 4 0 0 0 0 2 162 1 0.0 4 1 165 1 0.0 4 1 170 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 177 1 0.0 4 1 178 2 0.0 4 1 0 1 181 1 0.0 4 1 200 1 0.0 4 1 202 2 0.0 4 1 0 0 0 1 203 1 0.0 4 0 0 0 0 1 221 1 0.0 4 1 225 1 0.0 4 1 272 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.22 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 62,569 Reads with adapters: 5,817 (9.3%) Reads written (passing filters): 62,569 (100.0%) Total basepairs processed: 17,024,380 bp Total written (filtered): 16,960,316 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5817 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 2.8% G: 93.7% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 977.6 0 8 4 8 244.4 0 8 7 2 3.8 0 2 9 791 0.2 0 27 764 10 4584 0.1 1 185 4399 11 382 0.0 1 1 381 13 6 0.0 1 0 6 14 1 0.0 1 1 16 1 0.0 1 1 22 1 0.0 1 1 150 2 0.0 1 0 2 151 1 0.0 1 1 153 1 0.0 1 0 1 189 1 0.0 1 0 1 191 4 0.0 1 1 3 195 1 0.0 1 1 199 2 0.0 1 1 1 208 9 0.0 1 1 8 209 2 0.0 1 0 2 211 4 0.0 1 2 2 212 1 0.0 1 0 1 223 3 0.0 1 3 268 1 0.0 1 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.