This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GATATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.41 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 73,428 (2.6%) Reads that were too short: 13,351 (0.5%) Reads written (passing filters): 60,077 (2.1%) Total basepairs processed: 853,219,115 bp Total written (filtered): 15,278,160 bp (1.8%) === Adapter 1 === Sequence: GATATT; Type: regular 5'; Length: 6; Trimmed: 73428 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 353 692.0 0 353 7 17283 692.0 0 17283 8 845 692.0 0 845 9 15231 692.0 0 15231 10 115 692.0 0 115 11 111 692.0 0 111 12 111 692.0 0 111 13 87 692.0 0 87 14 74 692.0 0 74 15 88 692.0 0 88 16 105 692.0 0 105 17 103 692.0 0 103 18 78 692.0 0 78 19 101 692.0 0 101 20 113 692.0 0 113 21 151 692.0 0 151 22 184 692.0 0 184 23 80 692.0 0 80 24 113 692.0 0 113 25 137 692.0 0 137 26 126 692.0 0 126 27 91 692.0 0 91 28 105 692.0 0 105 29 107 692.0 0 107 30 112 692.0 0 112 31 122 692.0 0 122 32 115 692.0 0 115 33 172 692.0 0 172 34 275 692.0 0 275 35 1038 692.0 0 1038 36 1105 692.0 0 1105 37 1351 692.0 0 1351 38 109 692.0 0 109 39 90 692.0 0 90 40 78 692.0 0 78 41 115 692.0 0 115 42 101 692.0 0 101 43 76 692.0 0 76 44 99 692.0 0 99 45 86 692.0 0 86 46 95 692.0 0 95 47 84 692.0 0 84 48 88 692.0 0 88 49 92 692.0 0 92 50 99 692.0 0 99 51 178 692.0 0 178 52 89 692.0 0 89 53 81 692.0 0 81 54 81 692.0 0 81 55 74 692.0 0 74 56 99 692.0 0 99 57 79 692.0 0 79 58 60 692.0 0 60 59 76 692.0 0 76 60 119 692.0 0 119 61 76 692.0 0 76 62 88 692.0 0 88 63 69 692.0 0 69 64 85 692.0 0 85 65 65 692.0 0 65 66 73 692.0 0 73 67 98 692.0 0 98 68 152 692.0 0 152 69 93 692.0 0 93 70 122 692.0 0 122 71 105 692.0 0 105 72 65 692.0 0 65 73 100 692.0 0 100 74 85 692.0 0 85 75 83 692.0 0 83 76 114 692.0 0 114 77 100 692.0 0 100 78 152 692.0 0 152 79 111 692.0 0 111 80 144 692.0 0 144 81 115 692.0 0 115 82 103 692.0 0 103 83 99 692.0 0 99 84 115 692.0 0 115 85 159 692.0 0 159 86 91 692.0 0 91 87 125 692.0 0 125 88 358 692.0 0 358 89 86 692.0 0 86 90 163 692.0 0 163 91 99 692.0 0 99 92 91 692.0 0 91 93 94 692.0 0 94 94 114 692.0 0 114 95 157 692.0 0 157 96 200 692.0 0 200 97 145 692.0 0 145 98 155 692.0 0 155 99 439 692.0 0 439 100 212 692.0 0 212 101 203 692.0 0 203 102 99 692.0 0 99 103 123 692.0 0 123 104 105 692.0 0 105 105 108 692.0 0 108 106 103 692.0 0 103 107 136 692.0 0 136 108 118 692.0 0 118 109 144 692.0 0 144 110 287 692.0 0 287 111 554 692.0 0 554 112 990 692.0 0 990 113 169 692.0 0 169 114 110 692.0 0 110 115 88 692.0 0 88 116 117 692.0 0 117 117 86 692.0 0 86 118 96 692.0 0 96 119 118 692.0 0 118 120 115 692.0 0 115 121 259 692.0 0 259 122 151 692.0 0 151 123 71 692.0 0 71 124 130 692.0 0 130 125 104 692.0 0 104 126 95 692.0 0 95 127 159 692.0 0 159 128 149 692.0 0 149 129 114 692.0 0 114 130 95 692.0 0 95 131 177 692.0 0 177 132 109 692.0 0 109 133 159 692.0 0 159 134 95 692.0 0 95 135 106 692.0 0 106 136 114 692.0 0 114 137 109 692.0 0 109 138 90 692.0 0 90 139 208 692.0 0 208 140 138 692.0 0 138 141 141 692.0 0 141 142 91 692.0 0 91 143 96 692.0 0 96 144 66 692.0 0 66 145 75 692.0 0 75 146 85 692.0 0 85 147 245 692.0 0 245 148 188 692.0 0 188 149 217 692.0 0 217 150 73 692.0 0 73 151 79 692.0 0 79 152 89 692.0 0 89 153 109 692.0 0 109 154 92 692.0 0 92 155 107 692.0 0 107 156 70 692.0 0 70 157 93 692.0 0 93 158 95 692.0 0 95 159 144 692.0 0 144 160 159 692.0 0 159 161 291 692.0 0 291 162 57 692.0 0 57 163 67 692.0 0 67 164 95 692.0 0 95 165 83 692.0 0 83 166 100 692.0 0 100 167 85 692.0 0 85 168 108 692.0 0 108 169 87 692.0 0 87 170 71 692.0 0 71 171 77 692.0 0 77 172 99 692.0 0 99 173 84 692.0 0 84 174 96 692.0 0 96 175 86 692.0 0 86 176 75 692.0 0 75 177 72 692.0 0 72 178 80 692.0 0 80 179 99 692.0 0 99 180 68 692.0 0 68 181 94 692.0 0 94 182 102 692.0 0 102 183 85 692.0 0 85 184 91 692.0 0 91 185 112 692.0 0 112 186 84 692.0 0 84 187 91 692.0 0 91 188 82 692.0 0 82 189 90 692.0 0 90 190 102 692.0 0 102 191 114 692.0 0 114 192 80 692.0 0 80 193 87 692.0 0 87 194 118 692.0 0 118 195 74 692.0 0 74 196 67 692.0 0 67 197 94 692.0 0 94 198 73 692.0 0 73 199 82 692.0 0 82 200 110 692.0 0 110 201 120 692.0 0 120 202 106 692.0 0 106 203 103 692.0 0 103 204 105 692.0 0 105 205 206 692.0 0 206 206 280 692.0 0 280 207 261 692.0 0 261 208 183 692.0 0 183 209 130 692.0 0 130 210 102 692.0 0 102 211 125 692.0 0 125 212 109 692.0 0 109 213 115 692.0 0 115 214 112 692.0 0 112 215 83 692.0 0 83 216 70 692.0 0 70 217 80 692.0 0 80 218 88 692.0 0 88 219 99 692.0 0 99 220 103 692.0 0 103 221 118 692.0 0 118 222 130 692.0 0 130 223 101 692.0 0 101 224 124 692.0 0 124 225 90 692.0 0 90 226 67 692.0 0 67 227 105 692.0 0 105 228 87 692.0 0 87 229 108 692.0 0 108 230 114 692.0 0 114 231 172 692.0 0 172 232 198 692.0 0 198 233 156 692.0 0 156 234 225 692.0 0 225 235 337 692.0 0 337 236 278 692.0 0 278 237 1250 692.0 0 1250 238 161 692.0 0 161 239 149 692.0 0 149 240 87 692.0 0 87 241 133 692.0 0 133 242 153 692.0 0 153 243 114 692.0 0 114 244 76 692.0 0 76 245 67 692.0 0 67 246 78 692.0 0 78 247 76 692.0 0 76 248 121 692.0 0 121 249 141 692.0 0 141 250 239 692.0 0 239 251 84 692.0 0 84 252 77 692.0 0 77 253 81 692.0 0 81 254 122 692.0 0 122 255 147 692.0 0 147 256 196 692.0 0 196 257 220 692.0 0 220 258 168 692.0 0 168 259 210 692.0 0 210 260 266 692.0 0 266 261 382 692.0 0 382 262 241 692.0 0 241 263 89 692.0 0 89 264 68 692.0 0 68 265 74 692.0 0 74 266 86 692.0 0 86 267 58 692.0 0 58 268 71 692.0 0 71 269 62 692.0 0 62 270 87 692.0 0 87 271 65 692.0 0 65 272 87 692.0 0 87 273 111 692.0 0 111 274 97 692.0 0 97 275 166 692.0 0 166 276 90 692.0 0 90 277 71 692.0 0 71 278 79 692.0 0 79 279 84 692.0 0 84 280 166 692.0 0 166 281 84 692.0 0 84 282 143 692.0 0 143 283 86 692.0 0 86 284 79 692.0 0 79 285 67 692.0 0 67 286 64 692.0 0 64 287 66 692.0 0 66 288 107 692.0 0 107 289 88 692.0 0 88 290 82 692.0 0 82 291 88 692.0 0 88 292 50 692.0 0 50 293 79 692.0 0 79 294 101 692.0 0 101 295 111 692.0 0 111 296 77 692.0 0 77 297 119 692.0 0 119 298 70 692.0 0 70 299 82 692.0 0 82 300 98 692.0 0 98 301 90 692.0 0 90 Finished in 2.84 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 60,077 Reads with adapters: 16,030 (26.7%) Reads that were too short: 71 (0.1%) Reads written (passing filters): 15,959 (26.6%) Total basepairs processed: 15,278,160 bp Total written (filtered): 4,356,310 bp (28.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 16030 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 14 0.0 3 0 0 8 6 16 256 0.0 3 0 225 17 14 17 15061 0.0 3 14024 871 122 44 18 315 0.0 3 12 35 5 263 19 40 0.0 3 0 0 18 22 20 54 0.0 3 1 0 0 53 26 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 1 80 1 0.0 3 0 0 0 1 81 36 0.0 3 0 0 0 36 84 8 0.0 3 0 0 0 8 85 1 0.0 3 0 0 0 1 86 6 0.0 3 0 0 2 4 87 4 0.0 3 0 0 0 4 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 1 94 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 1 110 14 0.0 3 0 0 0 14 111 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 131 10 0.0 3 0 0 1 9 132 3 0.0 3 0 0 0 3 134 6 0.0 3 0 0 0 6 135 2 0.0 3 1 0 0 1 136 21 0.0 3 0 0 0 21 137 4 0.0 3 0 0 0 4 139 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 146 6 0.0 3 0 0 0 6 147 2 0.0 3 0 0 0 2 148 26 0.0 3 0 0 1 25 150 16 0.0 3 0 0 1 15 151 2 0.0 3 0 0 0 2 153 6 0.0 3 0 0 2 4 154 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 157 3 0.0 3 0 0 0 3 161 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 169 17 0.0 3 0 0 0 17 171 1 0.0 3 0 0 0 1 172 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 4 0.0 3 0 0 0 4 184 2 0.0 3 0 0 0 2 190 1 0.0 3 0 0 1 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 4 0.0 3 0 0 0 4 195 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 209 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 233 2 0.0 3 0 0 0 2 238 2 0.0 3 0 0 0 2 246 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 1 263 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 1 2 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.57 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 15,959 Reads with adapters: 340 (2.1%) Reads written (passing filters): 15,959 (100.0%) Total basepairs processed: 4,356,310 bp Total written (filtered): 4,355,256 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 340 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 44.4% C: 12.4% G: 40.9% T: 2.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 314 249.4 0 314 4 25 62.3 0 25 12 1 0.0 1 0 1