This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GATATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.52 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 68,307 (2.4%) Reads that were too short: 11,807 (0.4%) Reads written (passing filters): 56,500 (2.0%) Total basepairs processed: 853,219,115 bp Total written (filtered): 14,623,204 bp (1.7%) === Adapter 1 === Sequence: GATATT; Type: regular 5'; Length: 6; Trimmed: 68307 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 329 692.0 0 329 7 18124 692.0 0 18124 8 674 692.0 0 674 9 15658 692.0 0 15658 10 127 692.0 0 127 11 92 692.0 0 92 12 73 692.0 0 73 13 78 692.0 0 78 14 90 692.0 0 90 15 90 692.0 0 90 16 89 692.0 0 89 17 92 692.0 0 92 18 84 692.0 0 84 19 90 692.0 0 90 20 100 692.0 0 100 21 106 692.0 0 106 22 128 692.0 0 128 23 85 692.0 0 85 24 81 692.0 0 81 25 96 692.0 0 96 26 93 692.0 0 93 27 98 692.0 0 98 28 85 692.0 0 85 29 98 692.0 0 98 30 102 692.0 0 102 31 95 692.0 0 95 32 96 692.0 0 96 33 103 692.0 0 103 34 153 692.0 0 153 35 670 692.0 0 670 36 865 692.0 0 865 37 1252 692.0 0 1252 38 89 692.0 0 89 39 60 692.0 0 60 40 71 692.0 0 71 41 83 692.0 0 83 42 60 692.0 0 60 43 67 692.0 0 67 44 61 692.0 0 61 45 53 692.0 0 53 46 69 692.0 0 69 47 75 692.0 0 75 48 59 692.0 0 59 49 86 692.0 0 86 50 79 692.0 0 79 51 137 692.0 0 137 52 72 692.0 0 72 53 70 692.0 0 70 54 82 692.0 0 82 55 65 692.0 0 65 56 83 692.0 0 83 57 61 692.0 0 61 58 56 692.0 0 56 59 55 692.0 0 55 60 113 692.0 0 113 61 78 692.0 0 78 62 75 692.0 0 75 63 75 692.0 0 75 64 105 692.0 0 105 65 72 692.0 0 72 66 73 692.0 0 73 67 77 692.0 0 77 68 120 692.0 0 120 69 93 692.0 0 93 70 94 692.0 0 94 71 124 692.0 0 124 72 86 692.0 0 86 73 80 692.0 0 80 74 62 692.0 0 62 75 81 692.0 0 81 76 71 692.0 0 71 77 78 692.0 0 78 78 89 692.0 0 89 79 88 692.0 0 88 80 97 692.0 0 97 81 99 692.0 0 99 82 101 692.0 0 101 83 82 692.0 0 82 84 101 692.0 0 101 85 163 692.0 0 163 86 81 692.0 0 81 87 93 692.0 0 93 88 316 692.0 0 316 89 90 692.0 0 90 90 134 692.0 0 134 91 104 692.0 0 104 92 87 692.0 0 87 93 102 692.0 0 102 94 95 692.0 0 95 95 106 692.0 0 106 96 137 692.0 0 137 97 103 692.0 0 103 98 126 692.0 0 126 99 498 692.0 0 498 100 194 692.0 0 194 101 214 692.0 0 214 102 86 692.0 0 86 103 120 692.0 0 120 104 94 692.0 0 94 105 83 692.0 0 83 106 108 692.0 0 108 107 107 692.0 0 107 108 91 692.0 0 91 109 99 692.0 0 99 110 196 692.0 0 196 111 543 692.0 0 543 112 471 692.0 0 471 113 162 692.0 0 162 114 83 692.0 0 83 115 70 692.0 0 70 116 103 692.0 0 103 117 100 692.0 0 100 118 71 692.0 0 71 119 70 692.0 0 70 120 90 692.0 0 90 121 217 692.0 0 217 122 111 692.0 0 111 123 68 692.0 0 68 124 110 692.0 0 110 125 73 692.0 0 73 126 76 692.0 0 76 127 110 692.0 0 110 128 99 692.0 0 99 129 98 692.0 0 98 130 80 692.0 0 80 131 116 692.0 0 116 132 107 692.0 0 107 133 93 692.0 0 93 134 70 692.0 0 70 135 63 692.0 0 63 136 76 692.0 0 76 137 76 692.0 0 76 138 77 692.0 0 77 139 162 692.0 0 162 140 99 692.0 0 99 141 132 692.0 0 132 142 75 692.0 0 75 143 73 692.0 0 73 144 81 692.0 0 81 145 73 692.0 0 73 146 81 692.0 0 81 147 143 692.0 0 143 148 115 692.0 0 115 149 102 692.0 0 102 150 47 692.0 0 47 151 80 692.0 0 80 152 72 692.0 0 72 153 79 692.0 0 79 154 64 692.0 0 64 155 100 692.0 0 100 156 61 692.0 0 61 157 86 692.0 0 86 158 80 692.0 0 80 159 140 692.0 0 140 160 154 692.0 0 154 161 260 692.0 0 260 162 72 692.0 0 72 163 59 692.0 0 59 164 81 692.0 0 81 165 79 692.0 0 79 166 53 692.0 0 53 167 105 692.0 0 105 168 90 692.0 0 90 169 74 692.0 0 74 170 64 692.0 0 64 171 109 692.0 0 109 172 82 692.0 0 82 173 82 692.0 0 82 174 86 692.0 0 86 175 70 692.0 0 70 176 71 692.0 0 71 177 83 692.0 0 83 178 70 692.0 0 70 179 86 692.0 0 86 180 76 692.0 0 76 181 93 692.0 0 93 182 82 692.0 0 82 183 91 692.0 0 91 184 81 692.0 0 81 185 99 692.0 0 99 186 88 692.0 0 88 187 83 692.0 0 83 188 86 692.0 0 86 189 79 692.0 0 79 190 75 692.0 0 75 191 83 692.0 0 83 192 79 692.0 0 79 193 78 692.0 0 78 194 83 692.0 0 83 195 88 692.0 0 88 196 71 692.0 0 71 197 85 692.0 0 85 198 72 692.0 0 72 199 73 692.0 0 73 200 87 692.0 0 87 201 80 692.0 0 80 202 81 692.0 0 81 203 96 692.0 0 96 204 91 692.0 0 91 205 174 692.0 0 174 206 293 692.0 0 293 207 294 692.0 0 294 208 157 692.0 0 157 209 118 692.0 0 118 210 114 692.0 0 114 211 104 692.0 0 104 212 87 692.0 0 87 213 79 692.0 0 79 214 78 692.0 0 78 215 82 692.0 0 82 216 81 692.0 0 81 217 70 692.0 0 70 218 66 692.0 0 66 219 101 692.0 0 101 220 80 692.0 0 80 221 89 692.0 0 89 222 110 692.0 0 110 223 83 692.0 0 83 224 96 692.0 0 96 225 97 692.0 0 97 226 73 692.0 0 73 227 89 692.0 0 89 228 99 692.0 0 99 229 88 692.0 0 88 230 88 692.0 0 88 231 115 692.0 0 115 232 125 692.0 0 125 233 104 692.0 0 104 234 120 692.0 0 120 235 358 692.0 0 358 236 287 692.0 0 287 237 1335 692.0 0 1335 238 159 692.0 0 159 239 192 692.0 0 192 240 103 692.0 0 103 241 121 692.0 0 121 242 150 692.0 0 150 243 156 692.0 0 156 244 69 692.0 0 69 245 73 692.0 0 73 246 69 692.0 0 69 247 52 692.0 0 52 248 169 692.0 0 169 249 149 692.0 0 149 250 309 692.0 0 309 251 85 692.0 0 85 252 60 692.0 0 60 253 63 692.0 0 63 254 85 692.0 0 85 255 114 692.0 0 114 256 150 692.0 0 150 257 159 692.0 0 159 258 103 692.0 0 103 259 144 692.0 0 144 260 146 692.0 0 146 261 238 692.0 0 238 262 177 692.0 0 177 263 65 692.0 0 65 264 54 692.0 0 54 265 64 692.0 0 64 266 75 692.0 0 75 267 58 692.0 0 58 268 67 692.0 0 67 269 48 692.0 0 48 270 56 692.0 0 56 271 62 692.0 0 62 272 68 692.0 0 68 273 87 692.0 0 87 274 97 692.0 0 97 275 101 692.0 0 101 276 70 692.0 0 70 277 45 692.0 0 45 278 74 692.0 0 74 279 89 692.0 0 89 280 95 692.0 0 95 281 82 692.0 0 82 282 106 692.0 0 106 283 82 692.0 0 82 284 87 692.0 0 87 285 61 692.0 0 61 286 82 692.0 0 82 287 55 692.0 0 55 288 79 692.0 0 79 289 64 692.0 0 64 290 60 692.0 0 60 291 75 692.0 0 75 292 61 692.0 0 61 293 58 692.0 0 58 294 114 692.0 0 114 295 89 692.0 0 89 296 69 692.0 0 69 297 86 692.0 0 86 298 72 692.0 0 72 299 78 692.0 0 78 300 86 692.0 0 86 301 89 692.0 0 89 Finished in 2.98 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 56,500 Reads with adapters: 18,264 (32.3%) Reads that were too short: 15 (0.0%) Reads written (passing filters): 18,249 (32.3%) Total basepairs processed: 14,623,204 bp Total written (filtered): 4,977,562 bp (34.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 18264 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 7 0.0 3 0 0 5 1 1 19 184 0.0 3 0 173 8 0 3 20 17769 0.0 4 17379 369 19 1 1 21 61 0.0 4 22 36 2 1 22 4 0.0 4 1 0 3 23 20 0.0 4 2 0 0 17 1 24 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 40 1 0.0 4 1 62 1 0.0 4 0 0 0 0 1 78 1 0.0 4 1 89 2 0.0 4 0 0 0 0 2 98 1 0.0 4 0 0 0 0 1 99 4 0.0 4 0 0 0 0 4 126 2 0.0 4 0 0 0 0 2 127 174 0.0 4 0 0 0 0 174 133 2 0.0 4 2 135 2 0.0 4 2 136 3 0.0 4 3 137 5 0.0 4 3 0 1 1 147 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 190 3 0.0 4 0 0 0 0 3 215 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 1 228 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 233 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 279 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.63 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 18,249 Reads with adapters: 333 (1.8%) Reads written (passing filters): 18,249 (100.0%) Total basepairs processed: 4,977,562 bp Total written (filtered): 4,964,614 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 333 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 12.3% C: 8.7% G: 67.3% T: 11.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 36 285.1 0 36 4 4 71.3 0 4 9 3 0.1 0 0 3 10 8 0.0 1 0 8 11 186 0.0 1 2 184 12 37 0.0 1 0 37 14 1 0.0 1 0 1 15 3 0.0 1 0 3 124 21 0.0 1 18 3 153 1 0.0 1 0 1 210 1 0.0 1 1 225 31 0.0 1 27 4 226 1 0.0 1 0 1