This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.80 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 212,960 (7.5%) Reads that were too short: 9,556 (0.3%) Reads written (passing filters): 203,404 (7.2%) Total basepairs processed: 853,219,115 bp Total written (filtered): 49,084,322 bp (5.8%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 212960 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1176 692.0 0 1176 7 5196 692.0 0 5196 8 48940 692.0 0 48940 9 41707 692.0 0 41707 10 70 692.0 0 70 11 39 692.0 0 39 12 57 692.0 0 57 13 29 692.0 0 29 14 30 692.0 0 30 15 23 692.0 0 23 16 38 692.0 0 38 17 30 692.0 0 30 18 23 692.0 0 23 19 26 692.0 0 26 20 40 692.0 0 40 21 59 692.0 0 59 22 37 692.0 0 37 23 30 692.0 0 30 24 74 692.0 0 74 25 76 692.0 0 76 26 63 692.0 0 63 27 30 692.0 0 30 28 40 692.0 0 40 29 49 692.0 0 49 30 104 692.0 0 104 31 55 692.0 0 55 32 91 692.0 0 91 33 36 692.0 0 36 34 109 692.0 0 109 35 91 692.0 0 91 36 252 692.0 0 252 37 77 692.0 0 77 38 461 692.0 0 461 39 45 692.0 0 45 40 48 692.0 0 48 41 39 692.0 0 39 42 42 692.0 0 42 43 24 692.0 0 24 44 39 692.0 0 39 45 51 692.0 0 51 46 53 692.0 0 53 47 78 692.0 0 78 48 241 692.0 0 241 49 37 692.0 0 37 50 40 692.0 0 40 51 55 692.0 0 55 52 34 692.0 0 34 53 74 692.0 0 74 54 119 692.0 0 119 55 163 692.0 0 163 56 29 692.0 0 29 57 46 692.0 0 46 58 28 692.0 0 28 59 25 692.0 0 25 60 41 692.0 0 41 61 70 692.0 0 70 62 43 692.0 0 43 63 82 692.0 0 82 64 140 692.0 0 140 65 70 692.0 0 70 66 133 692.0 0 133 67 58 692.0 0 58 68 49 692.0 0 49 69 70 692.0 0 70 70 59 692.0 0 59 71 59 692.0 0 59 72 58 692.0 0 58 73 59 692.0 0 59 74 70 692.0 0 70 75 120 692.0 0 120 76 170 692.0 0 170 77 271 692.0 0 271 78 354 692.0 0 354 79 780 692.0 0 780 80 1742 692.0 0 1742 81 32653 692.0 0 32653 82 2613 692.0 0 2613 83 6744 692.0 0 6744 84 93 692.0 0 93 85 310 692.0 0 310 86 236 692.0 0 236 87 863 692.0 0 863 88 80 692.0 0 80 89 42 692.0 0 42 90 31 692.0 0 31 91 26 692.0 0 26 92 58 692.0 0 58 93 23 692.0 0 23 94 33 692.0 0 33 95 86 692.0 0 86 96 59 692.0 0 59 97 50 692.0 0 50 98 86 692.0 0 86 99 45 692.0 0 45 100 41 692.0 0 41 101 53 692.0 0 53 102 165 692.0 0 165 103 77 692.0 0 77 104 157 692.0 0 157 105 60 692.0 0 60 106 84 692.0 0 84 107 114 692.0 0 114 108 240 692.0 0 240 109 463 692.0 0 463 110 153 692.0 0 153 111 150 692.0 0 150 112 585 692.0 0 585 113 129 692.0 0 129 114 91 692.0 0 91 115 178 692.0 0 178 116 226 692.0 0 226 117 226 692.0 0 226 118 198 692.0 0 198 119 531 692.0 0 531 120 494 692.0 0 494 121 1082 692.0 0 1082 122 9538 692.0 0 9538 123 3398 692.0 0 3398 124 22635 692.0 0 22635 125 129 692.0 0 129 126 120 692.0 0 120 127 50 692.0 0 50 128 35 692.0 0 35 129 72 692.0 0 72 130 72 692.0 0 72 131 74 692.0 0 74 132 29 692.0 0 29 133 71 692.0 0 71 134 52 692.0 0 52 135 59 692.0 0 59 136 54 692.0 0 54 137 57 692.0 0 57 138 58 692.0 0 58 139 56 692.0 0 56 140 72 692.0 0 72 141 59 692.0 0 59 142 199 692.0 0 199 143 74 692.0 0 74 144 248 692.0 0 248 145 185 692.0 0 185 146 845 692.0 0 845 147 191 692.0 0 191 148 120 692.0 0 120 149 151 692.0 0 151 150 171 692.0 0 171 151 233 692.0 0 233 152 109 692.0 0 109 153 80 692.0 0 80 154 54 692.0 0 54 155 95 692.0 0 95 156 44 692.0 0 44 157 46 692.0 0 46 158 86 692.0 0 86 159 52 692.0 0 52 160 67 692.0 0 67 161 33 692.0 0 33 162 34 692.0 0 34 163 61 692.0 0 61 164 59 692.0 0 59 165 118 692.0 0 118 166 218 692.0 0 218 167 95 692.0 0 95 168 948 692.0 0 948 169 39 692.0 0 39 170 35 692.0 0 35 171 54 692.0 0 54 172 69 692.0 0 69 173 57 692.0 0 57 174 32 692.0 0 32 175 46 692.0 0 46 176 50 692.0 0 50 177 55 692.0 0 55 178 66 692.0 0 66 179 57 692.0 0 57 180 125 692.0 0 125 181 244 692.0 0 244 182 4193 692.0 0 4193 183 313 692.0 0 313 184 953 692.0 0 953 185 119 692.0 0 119 186 36 692.0 0 36 187 51 692.0 0 51 188 42 692.0 0 42 189 53 692.0 0 53 190 40 692.0 0 40 191 33 692.0 0 33 192 42 692.0 0 42 193 80 692.0 0 80 194 92 692.0 0 92 195 135 692.0 0 135 196 47 692.0 0 47 197 33 692.0 0 33 198 97 692.0 0 97 199 83 692.0 0 83 200 106 692.0 0 106 201 27 692.0 0 27 202 24 692.0 0 24 203 24 692.0 0 24 204 32 692.0 0 32 205 85 692.0 0 85 206 45 692.0 0 45 207 51 692.0 0 51 208 24 692.0 0 24 209 68 692.0 0 68 210 61 692.0 0 61 211 96 692.0 0 96 212 55 692.0 0 55 213 31 692.0 0 31 214 38 692.0 0 38 215 37 692.0 0 37 216 29 692.0 0 29 217 34 692.0 0 34 218 30 692.0 0 30 219 68 692.0 0 68 220 56 692.0 0 56 221 63 692.0 0 63 222 38 692.0 0 38 223 24 692.0 0 24 224 40 692.0 0 40 225 36 692.0 0 36 226 89 692.0 0 89 227 41 692.0 0 41 228 93 692.0 0 93 229 38 692.0 0 38 230 41 692.0 0 41 231 46 692.0 0 46 232 34 692.0 0 34 233 40 692.0 0 40 234 42 692.0 0 42 235 35 692.0 0 35 236 43 692.0 0 43 237 38 692.0 0 38 238 48 692.0 0 48 239 45 692.0 0 45 240 144 692.0 0 144 241 117 692.0 0 117 242 102 692.0 0 102 243 44 692.0 0 44 244 59 692.0 0 59 245 33 692.0 0 33 246 54 692.0 0 54 247 56 692.0 0 56 248 51 692.0 0 51 249 62 692.0 0 62 250 57 692.0 0 57 251 40 692.0 0 40 252 42 692.0 0 42 253 55 692.0 0 55 254 64 692.0 0 64 255 95 692.0 0 95 256 70 692.0 0 70 257 39 692.0 0 39 258 86 692.0 0 86 259 95 692.0 0 95 260 82 692.0 0 82 261 134 692.0 0 134 262 166 692.0 0 166 263 185 692.0 0 185 264 63 692.0 0 63 265 64 692.0 0 64 266 452 692.0 0 452 267 309 692.0 0 309 268 1109 692.0 0 1109 269 142 692.0 0 142 270 75 692.0 0 75 271 40 692.0 0 40 272 72 692.0 0 72 273 44 692.0 0 44 274 213 692.0 0 213 275 175 692.0 0 175 276 173 692.0 0 173 277 196 692.0 0 196 278 79 692.0 0 79 279 180 692.0 0 180 280 110 692.0 0 110 281 181 692.0 0 181 282 103 692.0 0 103 283 55 692.0 0 55 284 30 692.0 0 30 285 47 692.0 0 47 286 77 692.0 0 77 287 150 692.0 0 150 288 348 692.0 0 348 289 138 692.0 0 138 290 124 692.0 0 124 291 71 692.0 0 71 292 90 692.0 0 90 293 96 692.0 0 96 294 223 692.0 0 223 295 97 692.0 0 97 296 143 692.0 0 143 297 56 692.0 0 56 298 78 692.0 0 78 299 93 692.0 0 93 300 95 692.0 0 95 301 71 692.0 0 71 Finished in 8.83 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 203,404 Reads with adapters: 52,319 (25.7%) Reads that were too short: 1,167 (0.6%) Reads written (passing filters): 51,152 (25.1%) Total basepairs processed: 49,084,322 bp Total written (filtered): 13,925,137 bp (28.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 52319 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 12 0.0 2 0 0 0 12 15 35 0.0 3 0 0 24 11 16 429 0.0 3 0 339 59 31 17 48869 0.0 3 45519 2796 445 109 18 167 0.0 3 3 116 15 33 19 158 0.0 3 7 3 97 51 20 218 0.0 3 2 1 0 215 22 35 0.0 3 1 0 1 33 25 25 0.0 3 0 0 20 5 26 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 34 1030 0.0 3 0 0 1 1029 35 1 0.0 3 0 0 0 1 37 3 0.0 3 1 0 0 2 38 3 0.0 3 0 0 0 3 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 44 6 0.0 3 0 0 0 6 46 10 0.0 3 0 0 2 8 47 6 0.0 3 0 0 0 6 48 16 0.0 3 0 0 0 16 49 87 0.0 3 0 0 2 85 50 480 0.0 3 0 0 0 480 51 40 0.0 3 0 0 0 40 52 14 0.0 3 0 0 0 14 53 61 0.0 3 0 0 5 56 54 3 0.0 3 0 0 0 3 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 2 0.0 3 1 0 0 1 69 30 0.0 3 0 0 0 30 70 9 0.0 3 0 0 0 9 71 1 0.0 3 0 0 0 1 72 3 0.0 3 0 0 0 3 73 6 0.0 3 0 0 1 5 74 3 0.0 3 2 0 0 1 75 1 0.0 3 0 0 0 1 76 7 0.0 3 0 0 0 7 77 3 0.0 3 0 0 0 3 79 1 0.0 3 0 0 0 1 81 3 0.0 3 0 0 1 2 82 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 89 7 0.0 3 1 0 1 5 90 3 0.0 3 0 0 0 3 92 2 0.0 3 0 0 0 2 93 4 0.0 3 0 0 0 4 94 1 0.0 3 0 0 0 1 95 4 0.0 3 0 0 0 4 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 101 17 0.0 3 0 0 0 17 102 6 0.0 3 0 0 1 5 104 5 0.0 3 0 0 0 5 106 31 0.0 3 0 0 1 30 107 3 0.0 3 0 0 0 3 108 2 0.0 3 0 0 0 2 109 2 0.0 3 0 1 0 1 110 7 0.0 3 0 0 0 7 113 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 3 0.0 3 0 0 0 3 122 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 125 2 0.0 3 0 0 0 2 127 1 0.0 3 0 1 129 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 138 7 0.0 3 0 0 1 6 139 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 146 2 0.0 3 0 0 0 2 147 3 0.0 3 0 0 0 3 149 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 4 0.0 3 0 0 0 4 157 8 0.0 3 0 0 1 7 159 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 161 10 0.0 3 0 0 0 10 162 97 0.0 3 0 0 5 92 163 33 0.0 3 0 0 1 32 164 17 0.0 3 0 0 0 17 165 114 0.0 3 0 1 5 108 166 15 0.0 3 0 0 1 14 167 4 0.0 3 0 0 0 4 168 2 0.0 3 0 0 1 1 169 1 0.0 3 0 0 0 1 170 2 0.0 3 0 0 0 2 171 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 175 2 0.0 3 0 0 0 2 179 2 0.0 3 0 0 0 2 180 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 183 1 0.0 3 1 185 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 1 188 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 1 1 192 2 0.0 3 0 0 0 2 193 1 0.0 3 0 0 0 1 194 9 0.0 3 0 0 0 9 196 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 3 0.0 3 0 0 0 3 207 4 0.0 3 0 0 0 4 208 2 0.0 3 0 0 0 2 209 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 214 2 0.0 3 0 0 0 2 217 6 0.0 3 0 0 1 5 220 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 258 2 0.0 3 0 0 0 2 266 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 281 5 0.0 3 0 0 0 5 282 2 0.0 3 0 0 0 2 283 2 0.0 3 0 0 0 2 285 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.80 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 51,152 Reads with adapters: 461 (0.9%) Reads written (passing filters): 51,152 (100.0%) Total basepairs processed: 13,925,137 bp Total written (filtered): 13,923,647 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 461 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 47.3% C: 19.5% G: 29.7% T: 3.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 374 799.2 0 374 4 78 199.8 0 78 5 6 50.0 0 6 6 1 12.5 0 1 10 2 0.0 1 0 2