This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.27 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 222,591 (7.9%) Reads that were too short: 8,938 (0.3%) Reads written (passing filters): 213,653 (7.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 51,517,043 bp (6.0%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 222591 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1065 692.0 0 1065 7 4937 692.0 0 4937 8 45631 692.0 0 45631 9 50101 692.0 0 50101 10 89 692.0 0 89 11 34 692.0 0 34 12 27 692.0 0 27 13 37 692.0 0 37 14 19 692.0 0 19 15 22 692.0 0 22 16 34 692.0 0 34 17 29 692.0 0 29 18 35 692.0 0 35 19 28 692.0 0 28 20 28 692.0 0 28 21 39 692.0 0 39 22 47 692.0 0 47 23 32 692.0 0 32 24 34 692.0 0 34 25 38 692.0 0 38 26 36 692.0 0 36 27 22 692.0 0 22 28 52 692.0 0 52 29 41 692.0 0 41 30 99 692.0 0 99 31 45 692.0 0 45 32 89 692.0 0 89 33 37 692.0 0 37 34 79 692.0 0 79 35 70 692.0 0 70 36 183 692.0 0 183 37 77 692.0 0 77 38 512 692.0 0 512 39 61 692.0 0 61 40 37 692.0 0 37 41 40 692.0 0 40 42 73 692.0 0 73 43 31 692.0 0 31 44 39 692.0 0 39 45 41 692.0 0 41 46 71 692.0 0 71 47 66 692.0 0 66 48 294 692.0 0 294 49 45 692.0 0 45 50 36 692.0 0 36 51 63 692.0 0 63 52 41 692.0 0 41 53 62 692.0 0 62 54 130 692.0 0 130 55 81 692.0 0 81 56 44 692.0 0 44 57 44 692.0 0 44 58 38 692.0 0 38 59 45 692.0 0 45 60 63 692.0 0 63 61 81 692.0 0 81 62 56 692.0 0 56 63 74 692.0 0 74 64 152 692.0 0 152 65 89 692.0 0 89 66 104 692.0 0 104 67 71 692.0 0 71 68 68 692.0 0 68 69 77 692.0 0 77 70 64 692.0 0 64 71 72 692.0 0 72 72 64 692.0 0 64 73 78 692.0 0 78 74 78 692.0 0 78 75 95 692.0 0 95 76 158 692.0 0 158 77 263 692.0 0 263 78 311 692.0 0 311 79 725 692.0 0 725 80 1736 692.0 0 1736 81 34886 692.0 0 34886 82 2842 692.0 0 2842 83 7696 692.0 0 7696 84 84 692.0 0 84 85 174 692.0 0 174 86 138 692.0 0 138 87 258 692.0 0 258 88 62 692.0 0 62 89 42 692.0 0 42 90 66 692.0 0 66 91 33 692.0 0 33 92 46 692.0 0 46 93 46 692.0 0 46 94 45 692.0 0 45 95 43 692.0 0 43 96 70 692.0 0 70 97 55 692.0 0 55 98 106 692.0 0 106 99 44 692.0 0 44 100 58 692.0 0 58 101 84 692.0 0 84 102 121 692.0 0 121 103 74 692.0 0 74 104 116 692.0 0 116 105 86 692.0 0 86 106 109 692.0 0 109 107 138 692.0 0 138 108 310 692.0 0 310 109 498 692.0 0 498 110 174 692.0 0 174 111 179 692.0 0 179 112 645 692.0 0 645 113 139 692.0 0 139 114 116 692.0 0 116 115 194 692.0 0 194 116 285 692.0 0 285 117 198 692.0 0 198 118 157 692.0 0 157 119 475 692.0 0 475 120 428 692.0 0 428 121 703 692.0 0 703 122 10008 692.0 0 10008 123 3417 692.0 0 3417 124 25413 692.0 0 25413 125 129 692.0 0 129 126 94 692.0 0 94 127 51 692.0 0 51 128 40 692.0 0 40 129 75 692.0 0 75 130 60 692.0 0 60 131 80 692.0 0 80 132 30 692.0 0 30 133 46 692.0 0 46 134 54 692.0 0 54 135 49 692.0 0 49 136 49 692.0 0 49 137 41 692.0 0 41 138 51 692.0 0 51 139 67 692.0 0 67 140 46 692.0 0 46 141 42 692.0 0 42 142 207 692.0 0 207 143 68 692.0 0 68 144 203 692.0 0 203 145 170 692.0 0 170 146 670 692.0 0 670 147 168 692.0 0 168 148 82 692.0 0 82 149 76 692.0 0 76 150 154 692.0 0 154 151 101 692.0 0 101 152 98 692.0 0 98 153 85 692.0 0 85 154 47 692.0 0 47 155 66 692.0 0 66 156 40 692.0 0 40 157 41 692.0 0 41 158 86 692.0 0 86 159 44 692.0 0 44 160 62 692.0 0 62 161 54 692.0 0 54 162 44 692.0 0 44 163 58 692.0 0 58 164 39 692.0 0 39 165 147 692.0 0 147 166 256 692.0 0 256 167 89 692.0 0 89 168 1069 692.0 0 1069 169 56 692.0 0 56 170 44 692.0 0 44 171 43 692.0 0 43 172 74 692.0 0 74 173 44 692.0 0 44 174 30 692.0 0 30 175 55 692.0 0 55 176 56 692.0 0 56 177 55 692.0 0 55 178 66 692.0 0 66 179 70 692.0 0 70 180 159 692.0 0 159 181 244 692.0 0 244 182 4660 692.0 0 4660 183 360 692.0 0 360 184 1129 692.0 0 1129 185 106 692.0 0 106 186 62 692.0 0 62 187 66 692.0 0 66 188 39 692.0 0 39 189 46 692.0 0 46 190 49 692.0 0 49 191 35 692.0 0 35 192 32 692.0 0 32 193 55 692.0 0 55 194 41 692.0 0 41 195 70 692.0 0 70 196 39 692.0 0 39 197 39 692.0 0 39 198 66 692.0 0 66 199 51 692.0 0 51 200 71 692.0 0 71 201 43 692.0 0 43 202 38 692.0 0 38 203 31 692.0 0 31 204 37 692.0 0 37 205 60 692.0 0 60 206 55 692.0 0 55 207 34 692.0 0 34 208 39 692.0 0 39 209 50 692.0 0 50 210 36 692.0 0 36 211 43 692.0 0 43 212 59 692.0 0 59 213 38 692.0 0 38 214 51 692.0 0 51 215 43 692.0 0 43 216 33 692.0 0 33 217 50 692.0 0 50 218 38 692.0 0 38 219 67 692.0 0 67 220 73 692.0 0 73 221 58 692.0 0 58 222 40 692.0 0 40 223 39 692.0 0 39 224 46 692.0 0 46 225 41 692.0 0 41 226 41 692.0 0 41 227 47 692.0 0 47 228 90 692.0 0 90 229 51 692.0 0 51 230 58 692.0 0 58 231 41 692.0 0 41 232 43 692.0 0 43 233 47 692.0 0 47 234 42 692.0 0 42 235 46 692.0 0 46 236 41 692.0 0 41 237 58 692.0 0 58 238 56 692.0 0 56 239 48 692.0 0 48 240 146 692.0 0 146 241 134 692.0 0 134 242 68 692.0 0 68 243 35 692.0 0 35 244 38 692.0 0 38 245 30 692.0 0 30 246 42 692.0 0 42 247 59 692.0 0 59 248 58 692.0 0 58 249 66 692.0 0 66 250 66 692.0 0 66 251 41 692.0 0 41 252 64 692.0 0 64 253 75 692.0 0 75 254 57 692.0 0 57 255 83 692.0 0 83 256 72 692.0 0 72 257 46 692.0 0 46 258 90 692.0 0 90 259 71 692.0 0 71 260 93 692.0 0 93 261 131 692.0 0 131 262 163 692.0 0 163 263 127 692.0 0 127 264 73 692.0 0 73 265 87 692.0 0 87 266 514 692.0 0 514 267 326 692.0 0 326 268 1329 692.0 0 1329 269 87 692.0 0 87 270 46 692.0 0 46 271 39 692.0 0 39 272 61 692.0 0 61 273 34 692.0 0 34 274 230 692.0 0 230 275 119 692.0 0 119 276 158 692.0 0 158 277 85 692.0 0 85 278 55 692.0 0 55 279 71 692.0 0 71 280 47 692.0 0 47 281 118 692.0 0 118 282 83 692.0 0 83 283 66 692.0 0 66 284 42 692.0 0 42 285 34 692.0 0 34 286 66 692.0 0 66 287 118 692.0 0 118 288 241 692.0 0 241 289 116 692.0 0 116 290 96 692.0 0 96 291 55 692.0 0 55 292 95 692.0 0 95 293 104 692.0 0 104 294 159 692.0 0 159 295 91 692.0 0 91 296 107 692.0 0 107 297 59 692.0 0 59 298 65 692.0 0 65 299 62 692.0 0 62 300 65 692.0 0 65 301 72 692.0 0 72 Finished in 10.01 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 213,653 Reads with adapters: 56,642 (26.5%) Reads that were too short: 35 (0.0%) Reads written (passing filters): 56,607 (26.5%) Total basepairs processed: 51,517,043 bp Total written (filtered): 15,315,848 bp (29.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 56642 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 28 0.0 3 0 0 26 2 19 885 0.0 3 0 870 12 2 1 20 50565 0.0 4 49620 901 44 21 129 0.0 4 24 101 4 22 3848 0.0 4 3769 73 5 1 23 350 0.0 4 304 5 1 37 3 25 4 0.0 4 0 0 2 2 27 1 0.0 4 1 30 1 0.0 4 1 36 1 0.0 4 1 37 1 0.0 4 0 0 0 0 1 41 1 0.0 4 1 43 1 0.0 4 1 51 1 0.0 4 0 0 0 0 1 53 1 0.0 4 1 61 1 0.0 4 1 62 1 0.0 4 1 68 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 1 85 1 0.0 4 0 0 0 1 88 1 0.0 4 1 92 2 0.0 4 2 93 2 0.0 4 1 1 99 3 0.0 4 0 0 0 0 3 100 1 0.0 4 1 107 1 0.0 4 0 0 0 0 1 109 1 0.0 4 1 115 1 0.0 4 1 123 2 0.0 4 0 0 1 0 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 6 0.0 4 0 0 0 0 6 127 768 0.0 4 0 0 0 0 768 128 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 148 2 0.0 4 0 0 0 0 2 149 2 0.0 4 0 0 0 0 2 150 3 0.0 4 0 0 0 0 3 153 2 0.0 4 0 0 0 0 2 179 3 0.0 4 0 0 0 0 3 191 3 0.0 4 2 1 192 1 0.0 4 1 193 1 0.0 4 0 1 215 3 0.0 4 0 0 0 0 3 219 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.00 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 56,607 Reads with adapters: 6,768 (12.0%) Reads written (passing filters): 56,607 (100.0%) Total basepairs processed: 15,315,848 bp Total written (filtered): 15,242,967 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6768 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.3% C: 2.9% G: 94.3% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 884.5 0 6 4 8 221.1 0 8 5 1 55.3 0 1 7 1 3.5 0 1 8 1 0.9 0 1 9 2277 0.2 0 59 2218 10 3954 0.1 1 137 3817 11 472 0.0 1 0 472 12 5 0.0 1 0 5 13 3 0.0 1 0 3 84 1 0.0 1 1 138 1 0.0 1 1 150 1 0.0 1 0 1 169 15 0.0 1 15 189 1 0.0 1 0 1 200 13 0.0 1 13 203 1 0.0 1 1 208 2 0.0 1 1 1 209 2 0.0 1 2 232 1 0.0 1 0 1 257 1 0.0 1 0 1 265 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.