This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.57 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 172,315 (6.1%) Reads that were too short: 33,101 (1.2%) Reads written (passing filters): 139,214 (4.9%) Total basepairs processed: 853,219,115 bp Total written (filtered): 36,452,285 bp (4.3%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 172315 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1084 692.0 0 1084 7 6716 692.0 0 6716 8 54291 692.0 0 54291 9 42671 692.0 0 42671 10 78 692.0 0 78 11 18 692.0 0 18 12 23 692.0 0 23 13 18 692.0 0 18 14 18 692.0 0 18 15 16 692.0 0 16 16 17 692.0 0 17 17 23 692.0 0 23 18 21 692.0 0 21 19 12 692.0 0 12 20 22 692.0 0 22 21 10 692.0 0 10 22 12 692.0 0 12 23 20 692.0 0 20 24 21 692.0 0 21 25 36 692.0 0 36 26 16 692.0 0 16 27 30 692.0 0 30 28 19 692.0 0 19 29 18 692.0 0 18 30 21 692.0 0 21 31 18 692.0 0 18 32 68 692.0 0 68 33 48 692.0 0 48 34 43 692.0 0 43 35 29 692.0 0 29 36 52 692.0 0 52 37 192 692.0 0 192 38 165 692.0 0 165 39 185 692.0 0 185 40 83 692.0 0 83 41 54 692.0 0 54 42 526 692.0 0 526 43 14 692.0 0 14 44 31 692.0 0 31 45 17 692.0 0 17 46 31 692.0 0 31 47 30 692.0 0 30 48 24 692.0 0 24 49 33 692.0 0 33 50 23 692.0 0 23 51 39 692.0 0 39 52 416 692.0 0 416 53 140 692.0 0 140 54 217 692.0 0 217 55 27 692.0 0 27 56 13 692.0 0 13 57 21 692.0 0 21 58 20 692.0 0 20 59 23 692.0 0 23 60 44 692.0 0 44 61 64 692.0 0 64 62 56 692.0 0 56 63 18 692.0 0 18 64 19 692.0 0 19 65 39 692.0 0 39 66 77 692.0 0 77 67 30 692.0 0 30 68 92 692.0 0 92 69 16 692.0 0 16 70 17 692.0 0 17 71 54 692.0 0 54 72 83 692.0 0 83 73 133 692.0 0 133 74 15 692.0 0 15 75 19 692.0 0 19 76 12 692.0 0 12 77 24 692.0 0 24 78 12 692.0 0 12 79 10 692.0 0 10 80 15 692.0 0 15 81 30 692.0 0 30 82 19 692.0 0 19 83 18 692.0 0 18 84 88 692.0 0 88 85 90 692.0 0 90 86 114 692.0 0 114 87 36 692.0 0 36 88 21 692.0 0 21 89 18 692.0 0 18 90 85 692.0 0 85 91 81 692.0 0 81 92 74 692.0 0 74 93 137 692.0 0 137 94 98 692.0 0 98 95 165 692.0 0 165 96 57 692.0 0 57 97 62 692.0 0 62 98 32 692.0 0 32 99 17 692.0 0 17 100 20 692.0 0 20 101 17 692.0 0 17 102 19 692.0 0 19 103 24 692.0 0 24 104 75 692.0 0 75 105 76 692.0 0 76 106 60 692.0 0 60 107 70 692.0 0 70 108 217 692.0 0 217 109 269 692.0 0 269 110 222 692.0 0 222 111 112 692.0 0 112 112 200 692.0 0 200 113 289 692.0 0 289 114 30 692.0 0 30 115 41 692.0 0 41 116 32 692.0 0 32 117 98 692.0 0 98 118 72 692.0 0 72 119 94 692.0 0 94 120 25 692.0 0 25 121 23 692.0 0 23 122 26 692.0 0 26 123 33 692.0 0 33 124 46 692.0 0 46 125 68 692.0 0 68 126 97 692.0 0 97 127 566 692.0 0 566 128 113 692.0 0 113 129 302 692.0 0 302 130 37 692.0 0 37 131 39 692.0 0 39 132 60 692.0 0 60 133 91 692.0 0 91 134 41 692.0 0 41 135 29 692.0 0 29 136 39 692.0 0 39 137 42 692.0 0 42 138 61 692.0 0 61 139 124 692.0 0 124 140 101 692.0 0 101 141 142 692.0 0 142 142 294 692.0 0 294 143 447 692.0 0 447 144 7371 692.0 0 7371 145 3664 692.0 0 3664 146 8068 692.0 0 8068 147 83 692.0 0 83 148 45 692.0 0 45 149 37 692.0 0 37 150 89 692.0 0 89 151 562 692.0 0 562 152 305 692.0 0 305 153 261 692.0 0 261 154 126 692.0 0 126 155 68 692.0 0 68 156 195 692.0 0 195 157 128 692.0 0 128 158 167 692.0 0 167 159 63 692.0 0 63 160 36 692.0 0 36 161 80 692.0 0 80 162 55 692.0 0 55 163 25 692.0 0 25 164 31 692.0 0 31 165 37 692.0 0 37 166 26 692.0 0 26 167 37 692.0 0 37 168 20 692.0 0 20 169 21 692.0 0 21 170 41 692.0 0 41 171 84 692.0 0 84 172 111 692.0 0 111 173 92 692.0 0 92 174 38 692.0 0 38 175 31 692.0 0 31 176 34 692.0 0 34 177 94 692.0 0 94 178 55 692.0 0 55 179 705 692.0 0 705 180 104 692.0 0 104 181 153 692.0 0 153 182 30 692.0 0 30 183 32 692.0 0 32 184 36 692.0 0 36 185 52 692.0 0 52 186 78 692.0 0 78 187 33 692.0 0 33 188 32 692.0 0 32 189 25 692.0 0 25 190 99 692.0 0 99 191 29 692.0 0 29 192 162 692.0 0 162 193 47 692.0 0 47 194 36 692.0 0 36 195 45 692.0 0 45 196 37 692.0 0 37 197 73 692.0 0 73 198 223 692.0 0 223 199 65 692.0 0 65 200 83 692.0 0 83 201 68 692.0 0 68 202 31 692.0 0 31 203 28 692.0 0 28 204 36 692.0 0 36 205 93 692.0 0 93 206 70 692.0 0 70 207 45 692.0 0 45 208 30 692.0 0 30 209 21 692.0 0 21 210 15 692.0 0 15 211 33 692.0 0 33 212 40 692.0 0 40 213 29 692.0 0 29 214 39 692.0 0 39 215 30 692.0 0 30 216 35 692.0 0 35 217 130 692.0 0 130 218 70 692.0 0 70 219 66 692.0 0 66 220 30 692.0 0 30 221 25 692.0 0 25 222 32 692.0 0 32 223 30 692.0 0 30 224 25 692.0 0 25 225 91 692.0 0 91 226 56 692.0 0 56 227 60 692.0 0 60 228 164 692.0 0 164 229 94 692.0 0 94 230 196 692.0 0 196 231 91 692.0 0 91 232 75 692.0 0 75 233 91 692.0 0 91 234 81 692.0 0 81 235 280 692.0 0 280 236 961 692.0 0 961 237 445 692.0 0 445 238 467 692.0 0 467 239 127 692.0 0 127 240 726 692.0 0 726 241 256 692.0 0 256 242 237 692.0 0 237 243 69 692.0 0 69 244 56 692.0 0 56 245 110 692.0 0 110 246 123 692.0 0 123 247 336 692.0 0 336 248 139 692.0 0 139 249 159 692.0 0 159 250 378 692.0 0 378 251 261 692.0 0 261 252 623 692.0 0 623 253 156 692.0 0 156 254 64 692.0 0 64 255 79 692.0 0 79 256 129 692.0 0 129 257 131 692.0 0 131 258 215 692.0 0 215 259 378 692.0 0 378 260 5047 692.0 0 5047 261 5340 692.0 0 5340 262 4103 692.0 0 4103 263 655 692.0 0 655 264 50 692.0 0 50 265 55 692.0 0 55 266 102 692.0 0 102 267 155 692.0 0 155 268 160 692.0 0 160 269 82 692.0 0 82 270 444 692.0 0 444 271 123 692.0 0 123 272 522 692.0 0 522 273 72 692.0 0 72 274 45 692.0 0 45 275 54 692.0 0 54 276 94 692.0 0 94 277 137 692.0 0 137 278 163 692.0 0 163 279 183 692.0 0 183 280 352 692.0 0 352 281 267 692.0 0 267 282 274 692.0 0 274 283 120 692.0 0 120 284 167 692.0 0 167 285 642 692.0 0 642 286 117 692.0 0 117 287 121 692.0 0 121 288 591 692.0 0 591 289 187 692.0 0 187 290 300 692.0 0 300 291 621 692.0 0 621 292 509 692.0 0 509 293 773 692.0 0 773 294 669 692.0 0 669 295 326 692.0 0 326 296 330 692.0 0 330 297 57 692.0 0 57 298 47 692.0 0 47 299 43 692.0 0 43 300 52 692.0 0 52 301 63 692.0 0 63 Finished in 6.47 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 139,214 Reads with adapters: 59,482 (42.7%) Reads that were too short: 97 (0.1%) Reads written (passing filters): 59,385 (42.7%) Total basepairs processed: 36,452,285 bp Total written (filtered): 16,378,032 bp (44.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 59482 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 15 0.0 2 0 0 0 15 15 40 0.0 3 0 0 23 17 16 607 0.0 3 0 472 93 42 17 57836 0.0 3 54688 2507 499 142 18 206 0.0 3 6 119 32 49 19 36 0.0 3 2 4 12 18 20 530 0.0 3 1 0 0 529 21 1 0.0 3 1 22 1 0.0 3 1 23 6 0.0 3 0 0 3 3 25 5 0.0 3 0 0 2 3 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 34 2 0.0 3 0 0 1 1 40 3 0.0 3 0 0 0 3 41 2 0.0 3 0 0 0 2 42 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 55 5 0.0 3 0 0 0 5 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 60 3 0.0 3 0 0 0 3 62 5 0.0 3 0 0 0 5 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 67 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 3 0.0 3 0 0 0 3 79 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 5 0.0 3 0 1 0 4 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 101 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 126 2 0.0 3 0 1 0 1 138 1 0.0 3 0 0 1 142 4 0.0 3 0 0 0 4 143 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 147 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 151 17 0.0 3 0 0 0 17 152 3 0.0 3 0 0 0 3 154 3 0.0 3 0 0 0 3 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 163 3 0.0 3 0 0 0 3 165 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 11 0.0 3 0 0 0 11 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 192 9 0.0 3 0 0 0 9 194 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 231 1 0.0 3 1 236 2 0.0 3 0 0 0 2 237 1 0.0 3 0 1 238 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 1 252 3 0.0 3 0 0 0 3 253 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 279 6 0.0 3 0 0 0 6 280 1 0.0 3 0 0 0 1 281 6 0.0 3 0 0 0 6 282 8 0.0 3 0 0 0 8 285 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.11 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 59,385 Reads with adapters: 104 (0.2%) Reads written (passing filters): 59,385 (100.0%) Total basepairs processed: 16,378,032 bp Total written (filtered): 16,377,670 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 104 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.2% C: 14.4% G: 58.7% T: 6.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 60 927.9 0 60 4 40 232.0 0 40 5 3 58.0 0 3 7 1 3.6 0 1