This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.72 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 154,802 (5.5%) Reads that were too short: 16,035 (0.6%) Reads written (passing filters): 138,767 (4.9%) Total basepairs processed: 853,219,115 bp Total written (filtered): 36,693,434 bp (4.3%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 154802 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 538 692.0 0 538 7 3790 692.0 0 3790 8 43991 692.0 0 43991 9 60196 692.0 0 60196 10 64 692.0 0 64 11 15 692.0 0 15 12 6 692.0 0 6 13 7 692.0 0 7 14 5 692.0 0 5 15 5 692.0 0 5 16 12 692.0 0 12 17 7 692.0 0 7 18 8 692.0 0 8 19 3 692.0 0 3 20 9 692.0 0 9 21 3 692.0 0 3 22 12 692.0 0 12 23 10 692.0 0 10 24 13 692.0 0 13 25 9 692.0 0 9 26 6 692.0 0 6 27 10 692.0 0 10 28 15 692.0 0 15 29 9 692.0 0 9 30 10 692.0 0 10 31 13 692.0 0 13 32 26 692.0 0 26 33 18 692.0 0 18 34 29 692.0 0 29 35 19 692.0 0 19 36 18 692.0 0 18 37 159 692.0 0 159 38 87 692.0 0 87 39 94 692.0 0 94 40 12 692.0 0 12 41 12 692.0 0 12 42 118 692.0 0 118 43 6 692.0 0 6 44 10 692.0 0 10 45 7 692.0 0 7 46 8 692.0 0 8 47 10 692.0 0 10 48 11 692.0 0 11 49 18 692.0 0 18 50 13 692.0 0 13 51 25 692.0 0 25 52 177 692.0 0 177 53 128 692.0 0 128 54 95 692.0 0 95 55 40 692.0 0 40 56 6 692.0 0 6 57 20 692.0 0 20 58 8 692.0 0 8 59 10 692.0 0 10 60 330 692.0 0 330 61 87 692.0 0 87 62 24 692.0 0 24 63 7 692.0 0 7 64 13 692.0 0 13 65 8 692.0 0 8 66 19 692.0 0 19 67 12 692.0 0 12 68 16 692.0 0 16 69 10 692.0 0 10 70 7 692.0 0 7 71 23 692.0 0 23 72 28 692.0 0 28 73 110 692.0 0 110 74 6 692.0 0 6 75 9 692.0 0 9 76 9 692.0 0 9 77 6 692.0 0 6 78 8 692.0 0 8 79 12 692.0 0 12 80 16 692.0 0 16 81 20 692.0 0 20 82 21 692.0 0 21 83 13 692.0 0 13 84 49 692.0 0 49 85 29 692.0 0 29 86 51 692.0 0 51 87 17 692.0 0 17 88 11 692.0 0 11 89 14 692.0 0 14 90 49 692.0 0 49 91 32 692.0 0 32 92 24 692.0 0 24 93 26 692.0 0 26 94 26 692.0 0 26 95 47 692.0 0 47 96 40 692.0 0 40 97 47 692.0 0 47 98 16 692.0 0 16 99 12 692.0 0 12 100 10 692.0 0 10 101 10 692.0 0 10 102 11 692.0 0 11 103 24 692.0 0 24 104 47 692.0 0 47 105 57 692.0 0 57 106 45 692.0 0 45 107 42 692.0 0 42 108 70 692.0 0 70 109 70 692.0 0 70 110 71 692.0 0 71 111 32 692.0 0 32 112 64 692.0 0 64 113 69 692.0 0 69 114 32 692.0 0 32 115 21 692.0 0 21 116 19 692.0 0 19 117 91 692.0 0 91 118 30 692.0 0 30 119 25 692.0 0 25 120 10 692.0 0 10 121 18 692.0 0 18 122 14 692.0 0 14 123 11 692.0 0 11 124 31 692.0 0 31 125 38 692.0 0 38 126 70 692.0 0 70 127 530 692.0 0 530 128 115 692.0 0 115 129 189 692.0 0 189 130 33 692.0 0 33 131 29 692.0 0 29 132 53 692.0 0 53 133 76 692.0 0 76 134 33 692.0 0 33 135 31 692.0 0 31 136 34 692.0 0 34 137 45 692.0 0 45 138 56 692.0 0 56 139 75 692.0 0 75 140 99 692.0 0 99 141 131 692.0 0 131 142 368 692.0 0 368 143 481 692.0 0 481 144 8504 692.0 0 8504 145 4296 692.0 0 4296 146 9081 692.0 0 9081 147 73 692.0 0 73 148 36 692.0 0 36 149 13 692.0 0 13 150 33 692.0 0 33 151 167 692.0 0 167 152 66 692.0 0 66 153 71 692.0 0 71 154 42 692.0 0 42 155 18 692.0 0 18 156 63 692.0 0 63 157 52 692.0 0 52 158 84 692.0 0 84 159 19 692.0 0 19 160 22 692.0 0 22 161 29 692.0 0 29 162 45 692.0 0 45 163 14 692.0 0 14 164 15 692.0 0 15 165 20 692.0 0 20 166 16 692.0 0 16 167 22 692.0 0 22 168 13 692.0 0 13 169 19 692.0 0 19 170 14 692.0 0 14 171 25 692.0 0 25 172 40 692.0 0 40 173 34 692.0 0 34 174 14 692.0 0 14 175 15 692.0 0 15 176 25 692.0 0 25 177 35 692.0 0 35 178 41 692.0 0 41 179 286 692.0 0 286 180 63 692.0 0 63 181 76 692.0 0 76 182 25 692.0 0 25 183 8 692.0 0 8 184 19 692.0 0 19 185 12 692.0 0 12 186 18 692.0 0 18 187 9 692.0 0 9 188 12 692.0 0 12 189 13 692.0 0 13 190 65 692.0 0 65 191 26 692.0 0 26 192 99 692.0 0 99 193 24 692.0 0 24 194 15 692.0 0 15 195 16 692.0 0 16 196 21 692.0 0 21 197 32 692.0 0 32 198 111 692.0 0 111 199 30 692.0 0 30 200 39 692.0 0 39 201 8 692.0 0 8 202 12 692.0 0 12 203 15 692.0 0 15 204 15 692.0 0 15 205 24 692.0 0 24 206 24 692.0 0 24 207 13 692.0 0 13 208 11 692.0 0 11 209 10 692.0 0 10 210 7 692.0 0 7 211 11 692.0 0 11 212 23 692.0 0 23 213 15 692.0 0 15 214 23 692.0 0 23 215 16 692.0 0 16 216 26 692.0 0 26 217 172 692.0 0 172 218 50 692.0 0 50 219 30 692.0 0 30 220 14 692.0 0 14 221 8 692.0 0 8 222 13 692.0 0 13 223 23 692.0 0 23 224 25 692.0 0 25 225 37 692.0 0 37 226 35 692.0 0 35 227 35 692.0 0 35 228 54 692.0 0 54 229 58 692.0 0 58 230 136 692.0 0 136 231 45 692.0 0 45 232 63 692.0 0 63 233 38 692.0 0 38 234 36 692.0 0 36 235 142 692.0 0 142 236 406 692.0 0 406 237 173 692.0 0 173 238 162 692.0 0 162 239 50 692.0 0 50 240 344 692.0 0 344 241 135 692.0 0 135 242 140 692.0 0 140 243 34 692.0 0 34 244 24 692.0 0 24 245 51 692.0 0 51 246 46 692.0 0 46 247 160 692.0 0 160 248 66 692.0 0 66 249 50 692.0 0 50 250 124 692.0 0 124 251 131 692.0 0 131 252 223 692.0 0 223 253 63 692.0 0 63 254 25 692.0 0 25 255 30 692.0 0 30 256 53 692.0 0 53 257 57 692.0 0 57 258 110 692.0 0 110 259 167 692.0 0 167 260 2443 692.0 0 2443 261 2760 692.0 0 2760 262 1810 692.0 0 1810 263 406 692.0 0 406 264 21 692.0 0 21 265 27 692.0 0 27 266 50 692.0 0 50 267 81 692.0 0 81 268 74 692.0 0 74 269 68 692.0 0 68 270 541 692.0 0 541 271 58 692.0 0 58 272 129 692.0 0 129 273 48 692.0 0 48 274 27 692.0 0 27 275 25 692.0 0 25 276 35 692.0 0 35 277 75 692.0 0 75 278 63 692.0 0 63 279 57 692.0 0 57 280 80 692.0 0 80 281 72 692.0 0 72 282 57 692.0 0 57 283 129 692.0 0 129 284 139 692.0 0 139 285 1055 692.0 0 1055 286 59 692.0 0 59 287 55 692.0 0 55 288 308 692.0 0 308 289 92 692.0 0 92 290 146 692.0 0 146 291 157 692.0 0 157 292 112 692.0 0 112 293 222 692.0 0 222 294 130 692.0 0 130 295 121 692.0 0 121 296 87 692.0 0 87 297 31 692.0 0 31 298 18 692.0 0 18 299 24 692.0 0 24 300 23 692.0 0 23 301 37 692.0 0 37 Finished in 6.81 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 138,767 Reads with adapters: 63,057 (45.4%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 63,044 (45.4%) Total basepairs processed: 36,693,434 bp Total written (filtered): 17,139,243 bp (46.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 63057 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 29 0.0 3 0 0 29 19 836 0.0 3 0 818 14 3 1 20 61776 0.0 4 60614 1112 46 4 21 141 0.0 4 21 115 4 1 22 16 0.0 4 4 0 9 1 2 23 130 0.0 4 74 3 0 42 11 24 2 0.0 4 1 0 0 1 25 1 0.0 4 1 26 1 0.0 4 1 27 1 0.0 4 0 0 0 0 1 30 1 0.0 4 1 65 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 110 1 0.0 4 1 120 1 0.0 4 0 0 0 0 1 127 105 0.0 4 0 0 0 0 105 145 1 0.0 4 0 0 0 1 151 1 0.0 4 1 186 1 0.0 4 1 187 1 0.0 4 0 0 1 208 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 1 289 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.30 s (36 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 63,044 Reads with adapters: 5,966 (9.5%) Reads written (passing filters): 63,044 (100.0%) Total basepairs processed: 17,139,243 bp Total written (filtered): 17,071,024 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5966 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.3% C: 3.6% G: 92.2% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 12 985.1 0 12 4 8 246.3 0 8 5 6 61.6 0 6 7 1 3.8 0 1 9 659 0.2 0 18 641 10 4866 0.1 1 199 4667 11 352 0.0 1 3 349 12 3 0.0 1 0 3 13 3 0.0 1 0 3 16 3 0.0 1 3 17 1 0.0 1 1 18 1 0.0 1 1 23 4 0.0 1 4 60 1 0.0 1 1 150 4 0.0 1 0 4 189 1 0.0 1 0 1 208 36 0.0 1 7 29 209 4 0.0 1 0 4 228 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.