This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.44 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 97,151 (3.4%) Reads that were too short: 13,481 (0.5%) Reads written (passing filters): 83,670 (3.0%) Total basepairs processed: 853,219,115 bp Total written (filtered): 22,399,028 bp (2.6%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 97151 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 756 692.0 0 756 7 34668 692.0 0 34668 8 26971 692.0 0 26971 9 57 692.0 0 57 10 27 692.0 0 27 11 26 692.0 0 26 12 31 692.0 0 31 13 24 692.0 0 24 14 27 692.0 0 27 15 29 692.0 0 29 16 29 692.0 0 29 17 28 692.0 0 28 18 186 692.0 0 186 19 38 692.0 0 38 20 80 692.0 0 80 21 26 692.0 0 26 22 31 692.0 0 31 23 41 692.0 0 41 24 30 692.0 0 30 25 33 692.0 0 33 26 47 692.0 0 47 27 63 692.0 0 63 28 43 692.0 0 43 29 17 692.0 0 17 30 41 692.0 0 41 31 27 692.0 0 27 32 38 692.0 0 38 33 39 692.0 0 39 34 37 692.0 0 37 35 47 692.0 0 47 36 30 692.0 0 30 37 41 692.0 0 41 38 37 692.0 0 37 39 47 692.0 0 47 40 43 692.0 0 43 41 32 692.0 0 32 42 30 692.0 0 30 43 36 692.0 0 36 44 49 692.0 0 49 45 34 692.0 0 34 46 40 692.0 0 40 47 35 692.0 0 35 48 59 692.0 0 59 49 40 692.0 0 40 50 350 692.0 0 350 51 25 692.0 0 25 52 49 692.0 0 49 53 40 692.0 0 40 54 58 692.0 0 58 55 34 692.0 0 34 56 37 692.0 0 37 57 28 692.0 0 28 58 52 692.0 0 52 59 52 692.0 0 52 60 30 692.0 0 30 61 49 692.0 0 49 62 42 692.0 0 42 63 35 692.0 0 35 64 30 692.0 0 30 65 64 692.0 0 64 66 81 692.0 0 81 67 83 692.0 0 83 68 77 692.0 0 77 69 127 692.0 0 127 70 143 692.0 0 143 71 1026 692.0 0 1026 72 3128 692.0 0 3128 73 777 692.0 0 777 74 54 692.0 0 54 75 45 692.0 0 45 76 39 692.0 0 39 77 80 692.0 0 80 78 44 692.0 0 44 79 72 692.0 0 72 80 73 692.0 0 73 81 67 692.0 0 67 82 60 692.0 0 60 83 49 692.0 0 49 84 74 692.0 0 74 85 101 692.0 0 101 86 62 692.0 0 62 87 94 692.0 0 94 88 37 692.0 0 37 89 47 692.0 0 47 90 67 692.0 0 67 91 64 692.0 0 64 92 59 692.0 0 59 93 77 692.0 0 77 94 121 692.0 0 121 95 147 692.0 0 147 96 1138 692.0 0 1138 97 939 692.0 0 939 98 264 692.0 0 264 99 81 692.0 0 81 100 27 692.0 0 27 101 29 692.0 0 29 102 38 692.0 0 38 103 53 692.0 0 53 104 65 692.0 0 65 105 45 692.0 0 45 106 124 692.0 0 124 107 43 692.0 0 43 108 53 692.0 0 53 109 110 692.0 0 110 110 47 692.0 0 47 111 40 692.0 0 40 112 61 692.0 0 61 113 53 692.0 0 53 114 58 692.0 0 58 115 55 692.0 0 55 116 53 692.0 0 53 117 53 692.0 0 53 118 68 692.0 0 68 119 46 692.0 0 46 120 39 692.0 0 39 121 35 692.0 0 35 122 42 692.0 0 42 123 49 692.0 0 49 124 154 692.0 0 154 125 99 692.0 0 99 126 193 692.0 0 193 127 109 692.0 0 109 128 152 692.0 0 152 129 102 692.0 0 102 130 117 692.0 0 117 131 87 692.0 0 87 132 115 692.0 0 115 133 78 692.0 0 78 134 75 692.0 0 75 135 115 692.0 0 115 136 66 692.0 0 66 137 67 692.0 0 67 138 63 692.0 0 63 139 35 692.0 0 35 140 67 692.0 0 67 141 60 692.0 0 60 142 57 692.0 0 57 143 74 692.0 0 74 144 153 692.0 0 153 145 121 692.0 0 121 146 77 692.0 0 77 147 49 692.0 0 49 148 44 692.0 0 44 149 46 692.0 0 46 150 62 692.0 0 62 151 56 692.0 0 56 152 90 692.0 0 90 153 51 692.0 0 51 154 73 692.0 0 73 155 108 692.0 0 108 156 60 692.0 0 60 157 37 692.0 0 37 158 47 692.0 0 47 159 52 692.0 0 52 160 48 692.0 0 48 161 34 692.0 0 34 162 63 692.0 0 63 163 44 692.0 0 44 164 61 692.0 0 61 165 98 692.0 0 98 166 64 692.0 0 64 167 58 692.0 0 58 168 43 692.0 0 43 169 55 692.0 0 55 170 51 692.0 0 51 171 61 692.0 0 61 172 118 692.0 0 118 173 352 692.0 0 352 174 184 692.0 0 184 175 255 692.0 0 255 176 82 692.0 0 82 177 73 692.0 0 73 178 49 692.0 0 49 179 58 692.0 0 58 180 69 692.0 0 69 181 55 692.0 0 55 182 51 692.0 0 51 183 66 692.0 0 66 184 46 692.0 0 46 185 95 692.0 0 95 186 95 692.0 0 95 187 74 692.0 0 74 188 62 692.0 0 62 189 54 692.0 0 54 190 98 692.0 0 98 191 121 692.0 0 121 192 608 692.0 0 608 193 596 692.0 0 596 194 398 692.0 0 398 195 142 692.0 0 142 196 57 692.0 0 57 197 46 692.0 0 46 198 61 692.0 0 61 199 80 692.0 0 80 200 117 692.0 0 117 201 69 692.0 0 69 202 58 692.0 0 58 203 65 692.0 0 65 204 621 692.0 0 621 205 70 692.0 0 70 206 122 692.0 0 122 207 108 692.0 0 108 208 73 692.0 0 73 209 149 692.0 0 149 210 133 692.0 0 133 211 216 692.0 0 216 212 139 692.0 0 139 213 59 692.0 0 59 214 58 692.0 0 58 215 149 692.0 0 149 216 58 692.0 0 58 217 102 692.0 0 102 218 95 692.0 0 95 219 106 692.0 0 106 220 137 692.0 0 137 221 158 692.0 0 158 222 78 692.0 0 78 223 49 692.0 0 49 224 52 692.0 0 52 225 79 692.0 0 79 226 135 692.0 0 135 227 105 692.0 0 105 228 130 692.0 0 130 229 67 692.0 0 67 230 62 692.0 0 62 231 74 692.0 0 74 232 58 692.0 0 58 233 69 692.0 0 69 234 74 692.0 0 74 235 76 692.0 0 76 236 95 692.0 0 95 237 99 692.0 0 99 238 98 692.0 0 98 239 60 692.0 0 60 240 98 692.0 0 98 241 104 692.0 0 104 242 120 692.0 0 120 243 120 692.0 0 120 244 59 692.0 0 59 245 75 692.0 0 75 246 89 692.0 0 89 247 170 692.0 0 170 248 75 692.0 0 75 249 146 692.0 0 146 250 80 692.0 0 80 251 77 692.0 0 77 252 57 692.0 0 57 253 65 692.0 0 65 254 112 692.0 0 112 255 151 692.0 0 151 256 118 692.0 0 118 257 111 692.0 0 111 258 131 692.0 0 131 259 124 692.0 0 124 260 105 692.0 0 105 261 86 692.0 0 86 262 97 692.0 0 97 263 79 692.0 0 79 264 67 692.0 0 67 265 74 692.0 0 74 266 138 692.0 0 138 267 208 692.0 0 208 268 178 692.0 0 178 269 108 692.0 0 108 270 175 692.0 0 175 271 146 692.0 0 146 272 564 692.0 0 564 273 293 692.0 0 293 274 802 692.0 0 802 275 138 692.0 0 138 276 118 692.0 0 118 277 515 692.0 0 515 278 103 692.0 0 103 279 119 692.0 0 119 280 130 692.0 0 130 281 136 692.0 0 136 282 89 692.0 0 89 283 70 692.0 0 70 284 77 692.0 0 77 285 112 692.0 0 112 286 169 692.0 0 169 287 125 692.0 0 125 288 179 692.0 0 179 289 116 692.0 0 116 290 126 692.0 0 126 291 117 692.0 0 117 292 116 692.0 0 116 293 145 692.0 0 145 294 319 692.0 0 319 295 235 692.0 0 235 296 411 692.0 0 411 297 109 692.0 0 109 298 72 692.0 0 72 299 92 692.0 0 92 300 134 692.0 0 134 301 71 692.0 0 71 Finished in 4.00 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 83,670 Reads with adapters: 34,214 (40.9%) Reads that were too short: 87 (0.1%) Reads written (passing filters): 34,127 (40.8%) Total basepairs processed: 22,399,028 bp Total written (filtered): 9,435,663 bp (42.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 34214 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 22 0.0 3 0 0 9 13 16 295 0.0 3 0 236 41 18 17 31791 0.0 3 29637 1769 342 43 18 150 0.0 3 3 54 16 77 19 1512 0.0 3 1351 92 42 27 20 257 0.0 3 1 7 2 247 21 4 0.0 3 0 0 2 2 22 3 0.0 3 1 0 0 2 23 1 0.0 3 0 0 0 1 25 2 0.0 3 0 0 0 2 26 1 0.0 3 0 0 0 1 27 1 0.0 3 1 31 1 0.0 3 0 0 0 1 32 3 0.0 3 0 0 0 3 34 2 0.0 3 0 0 0 2 35 1 0.0 3 1 37 1 0.0 3 0 0 0 1 38 7 0.0 3 0 0 0 7 39 2 0.0 3 0 0 0 2 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 47 3 0.0 3 0 0 0 3 49 3 0.0 3 0 0 0 3 50 1 0.0 3 0 0 1 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 60 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 3 0.0 3 0 0 0 3 65 2 0.0 3 0 0 0 2 67 7 0.0 3 0 0 0 7 68 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 2 0.0 3 1 1 84 1 0.0 3 0 0 0 1 85 4 0.0 3 0 0 0 4 86 1 0.0 3 0 0 1 93 1 0.0 3 0 0 0 1 96 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 106 4 0.0 3 0 0 0 4 107 2 0.0 3 0 1 0 1 108 3 0.0 3 0 0 0 3 109 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 119 3 0.0 3 2 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 1 125 3 0.0 3 0 0 0 3 126 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 1 146 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 169 2 0.0 3 0 0 0 2 170 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 181 3 0.0 3 0 0 1 2 184 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 0 2 188 1 0.0 3 0 0 1 189 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 192 2 0.0 3 0 0 0 2 193 1 0.0 3 0 0 0 1 194 6 0.0 3 0 0 0 6 195 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 216 3 0.0 3 0 0 0 3 217 2 0.0 3 0 0 0 2 218 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 0 1 228 4 0.0 3 0 0 0 4 231 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 270 3 0.0 3 0 0 0 3 279 5 0.0 3 0 0 0 5 280 3 0.0 3 0 0 0 3 281 5 0.0 3 0 0 0 5 282 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.20 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 34,127 Reads with adapters: 212 (0.6%) Reads written (passing filters): 34,127 (100.0%) Total basepairs processed: 9,435,663 bp Total written (filtered): 9,435,010 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 212 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 28.3% C: 41.5% G: 29.2% T: 0.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 201 533.2 0 201 4 6 133.3 0 6 5 4 33.3 0 4 6 1 8.3 0 1