This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.21 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 90,237 (3.2%) Reads that were too short: 7,896 (0.3%) Reads written (passing filters): 82,341 (2.9%) Total basepairs processed: 853,219,115 bp Total written (filtered): 22,431,024 bp (2.6%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 90237 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 627 692.0 0 627 7 31397 692.0 0 31397 8 32024 692.0 0 32024 9 43 692.0 0 43 10 31 692.0 0 31 11 20 692.0 0 20 12 19 692.0 0 19 13 28 692.0 0 28 14 25 692.0 0 25 15 25 692.0 0 25 16 14 692.0 0 14 17 25 692.0 0 25 18 51 692.0 0 51 19 29 692.0 0 29 20 53 692.0 0 53 21 19 692.0 0 19 22 28 692.0 0 28 23 22 692.0 0 22 24 20 692.0 0 20 25 21 692.0 0 21 26 26 692.0 0 26 27 21 692.0 0 21 28 20 692.0 0 20 29 21 692.0 0 21 30 41 692.0 0 41 31 31 692.0 0 31 32 28 692.0 0 28 33 42 692.0 0 42 34 47 692.0 0 47 35 45 692.0 0 45 36 47 692.0 0 47 37 49 692.0 0 49 38 36 692.0 0 36 39 49 692.0 0 49 40 24 692.0 0 24 41 40 692.0 0 40 42 37 692.0 0 37 43 29 692.0 0 29 44 55 692.0 0 55 45 29 692.0 0 29 46 38 692.0 0 38 47 39 692.0 0 39 48 75 692.0 0 75 49 42 692.0 0 42 50 360 692.0 0 360 51 43 692.0 0 43 52 53 692.0 0 53 53 28 692.0 0 28 54 45 692.0 0 45 55 36 692.0 0 36 56 43 692.0 0 43 57 30 692.0 0 30 58 39 692.0 0 39 59 56 692.0 0 56 60 35 692.0 0 35 61 44 692.0 0 44 62 34 692.0 0 34 63 28 692.0 0 28 64 36 692.0 0 36 65 47 692.0 0 47 66 57 692.0 0 57 67 56 692.0 0 56 68 63 692.0 0 63 69 142 692.0 0 142 70 164 692.0 0 164 71 1095 692.0 0 1095 72 3510 692.0 0 3510 73 906 692.0 0 906 74 34 692.0 0 34 75 34 692.0 0 34 76 22 692.0 0 22 77 63 692.0 0 63 78 33 692.0 0 33 79 67 692.0 0 67 80 46 692.0 0 46 81 39 692.0 0 39 82 29 692.0 0 29 83 26 692.0 0 26 84 24 692.0 0 24 85 44 692.0 0 44 86 49 692.0 0 49 87 57 692.0 0 57 88 37 692.0 0 37 89 64 692.0 0 64 90 37 692.0 0 37 91 51 692.0 0 51 92 42 692.0 0 42 93 66 692.0 0 66 94 85 692.0 0 85 95 101 692.0 0 101 96 1070 692.0 0 1070 97 1121 692.0 0 1121 98 243 692.0 0 243 99 81 692.0 0 81 100 32 692.0 0 32 101 34 692.0 0 34 102 38 692.0 0 38 103 37 692.0 0 37 104 56 692.0 0 56 105 37 692.0 0 37 106 89 692.0 0 89 107 40 692.0 0 40 108 46 692.0 0 46 109 89 692.0 0 89 110 26 692.0 0 26 111 29 692.0 0 29 112 27 692.0 0 27 113 29 692.0 0 29 114 45 692.0 0 45 115 59 692.0 0 59 116 44 692.0 0 44 117 29 692.0 0 29 118 56 692.0 0 56 119 36 692.0 0 36 120 25 692.0 0 25 121 26 692.0 0 26 122 36 692.0 0 36 123 52 692.0 0 52 124 59 692.0 0 59 125 66 692.0 0 66 126 87 692.0 0 87 127 73 692.0 0 73 128 56 692.0 0 56 129 47 692.0 0 47 130 73 692.0 0 73 131 54 692.0 0 54 132 104 692.0 0 104 133 53 692.0 0 53 134 62 692.0 0 62 135 71 692.0 0 71 136 42 692.0 0 42 137 58 692.0 0 58 138 46 692.0 0 46 139 36 692.0 0 36 140 45 692.0 0 45 141 45 692.0 0 45 142 54 692.0 0 54 143 73 692.0 0 73 144 99 692.0 0 99 145 83 692.0 0 83 146 44 692.0 0 44 147 40 692.0 0 40 148 32 692.0 0 32 149 32 692.0 0 32 150 33 692.0 0 33 151 40 692.0 0 40 152 105 692.0 0 105 153 39 692.0 0 39 154 49 692.0 0 49 155 45 692.0 0 45 156 32 692.0 0 32 157 56 692.0 0 56 158 42 692.0 0 42 159 23 692.0 0 23 160 68 692.0 0 68 161 38 692.0 0 38 162 40 692.0 0 40 163 40 692.0 0 40 164 43 692.0 0 43 165 52 692.0 0 52 166 50 692.0 0 50 167 42 692.0 0 42 168 38 692.0 0 38 169 36 692.0 0 36 170 36 692.0 0 36 171 49 692.0 0 49 172 66 692.0 0 66 173 193 692.0 0 193 174 100 692.0 0 100 175 155 692.0 0 155 176 37 692.0 0 37 177 50 692.0 0 50 178 26 692.0 0 26 179 49 692.0 0 49 180 40 692.0 0 40 181 41 692.0 0 41 182 42 692.0 0 42 183 43 692.0 0 43 184 40 692.0 0 40 185 49 692.0 0 49 186 44 692.0 0 44 187 42 692.0 0 42 188 48 692.0 0 48 189 56 692.0 0 56 190 57 692.0 0 57 191 85 692.0 0 85 192 486 692.0 0 486 193 486 692.0 0 486 194 254 692.0 0 254 195 105 692.0 0 105 196 51 692.0 0 51 197 42 692.0 0 42 198 28 692.0 0 28 199 40 692.0 0 40 200 48 692.0 0 48 201 44 692.0 0 44 202 43 692.0 0 43 203 53 692.0 0 53 204 639 692.0 0 639 205 48 692.0 0 48 206 82 692.0 0 82 207 67 692.0 0 67 208 35 692.0 0 35 209 67 692.0 0 67 210 47 692.0 0 47 211 74 692.0 0 74 212 53 692.0 0 53 213 49 692.0 0 49 214 29 692.0 0 29 215 115 692.0 0 115 216 32 692.0 0 32 217 55 692.0 0 55 218 58 692.0 0 58 219 47 692.0 0 47 220 59 692.0 0 59 221 49 692.0 0 49 222 34 692.0 0 34 223 38 692.0 0 38 224 32 692.0 0 32 225 84 692.0 0 84 226 98 692.0 0 98 227 58 692.0 0 58 228 39 692.0 0 39 229 52 692.0 0 52 230 48 692.0 0 48 231 46 692.0 0 46 232 31 692.0 0 31 233 43 692.0 0 43 234 42 692.0 0 42 235 54 692.0 0 54 236 48 692.0 0 48 237 57 692.0 0 57 238 60 692.0 0 60 239 32 692.0 0 32 240 41 692.0 0 41 241 37 692.0 0 37 242 41 692.0 0 41 243 45 692.0 0 45 244 24 692.0 0 24 245 28 692.0 0 28 246 39 692.0 0 39 247 89 692.0 0 89 248 42 692.0 0 42 249 75 692.0 0 75 250 52 692.0 0 52 251 32 692.0 0 32 252 45 692.0 0 45 253 53 692.0 0 53 254 77 692.0 0 77 255 53 692.0 0 53 256 58 692.0 0 58 257 61 692.0 0 61 258 61 692.0 0 61 259 67 692.0 0 67 260 55 692.0 0 55 261 49 692.0 0 49 262 46 692.0 0 46 263 54 692.0 0 54 264 34 692.0 0 34 265 61 692.0 0 61 266 117 692.0 0 117 267 99 692.0 0 99 268 70 692.0 0 70 269 71 692.0 0 71 270 95 692.0 0 95 271 93 692.0 0 93 272 546 692.0 0 546 273 133 692.0 0 133 274 322 692.0 0 322 275 68 692.0 0 68 276 56 692.0 0 56 277 644 692.0 0 644 278 83 692.0 0 83 279 53 692.0 0 53 280 67 692.0 0 67 281 57 692.0 0 57 282 46 692.0 0 46 283 43 692.0 0 43 284 42 692.0 0 42 285 57 692.0 0 57 286 72 692.0 0 72 287 73 692.0 0 73 288 104 692.0 0 104 289 52 692.0 0 52 290 88 692.0 0 88 291 67 692.0 0 67 292 56 692.0 0 56 293 62 692.0 0 62 294 104 692.0 0 104 295 95 692.0 0 95 296 129 692.0 0 129 297 48 692.0 0 48 298 52 692.0 0 52 299 46 692.0 0 46 300 80 692.0 0 80 301 90 692.0 0 90 Finished in 4.29 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 82,341 Reads with adapters: 33,794 (41.0%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 33,778 (41.0%) Total basepairs processed: 22,431,024 bp Total written (filtered): 9,162,674 bp (40.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 33794 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 11 0.0 3 0 0 0 2 9 18 22 0.0 3 0 0 22 19 604 0.0 3 0 593 6 4 1 20 32473 0.0 4 31938 518 14 2 1 21 54 0.0 4 5 48 1 22 9 0.0 4 3 0 6 23 34 0.0 4 1 0 0 29 4 24 2 0.0 4 1 0 0 1 26 1 0.0 4 0 0 0 0 1 27 1 0.0 4 1 43 1 0.0 4 1 46 1 0.0 4 1 53 1 0.0 4 0 0 0 1 55 1 0.0 4 1 93 1 0.0 4 0 0 0 0 1 101 1 0.0 4 1 117 1 0.0 4 1 121 1 0.0 4 1 122 1 0.0 4 1 123 1 0.0 4 1 124 1 0.0 4 1 125 2 0.0 4 0 0 0 0 2 126 6 0.0 4 0 0 0 0 6 127 544 0.0 4 0 0 0 0 544 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 131 1 0.0 4 1 132 1 0.0 4 0 0 0 0 1 135 2 0.0 4 2 148 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 1 4 229 1 0.0 4 0 0 0 1 232 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.18 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 33,778 Reads with adapters: 2,549 (7.5%) Reads written (passing filters): 33,778 (100.0%) Total basepairs processed: 9,162,674 bp Total written (filtered): 9,127,918 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2549 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.8% C: 4.5% G: 91.1% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 19 527.8 0 19 4 5 131.9 0 5 5 2 33.0 0 2 9 68 0.1 0 1 67 10 442 0.0 1 16 426 11 1968 0.0 1 20 1948 12 6 0.0 1 0 6 13 1 0.0 1 0 1 15 1 0.0 1 0 1 151 4 0.0 1 0 4 152 1 0.0 1 0 1 170 1 0.0 1 1 209 2 0.0 1 0 2 224 28 0.0 1 25 3 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.