This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.73 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 220,002 (7.8%) Reads that were too short: 3,764 (0.1%) Reads written (passing filters): 216,238 (7.6%) Total basepairs processed: 853,219,115 bp Total written (filtered): 61,302,552 bp (7.2%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 220002 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1484 692.0 0 1484 7 110273 692.0 0 110273 8 1738 692.0 0 1738 9 79086 692.0 0 79086 10 57 692.0 0 57 11 83 692.0 0 83 12 31 692.0 0 31 13 40 692.0 0 40 14 31 692.0 0 31 15 30 692.0 0 30 16 70 692.0 0 70 17 54 692.0 0 54 18 145 692.0 0 145 19 25 692.0 0 25 20 28 692.0 0 28 21 25 692.0 0 25 22 26 692.0 0 26 23 32 692.0 0 32 24 22 692.0 0 22 25 31 692.0 0 31 26 30 692.0 0 30 27 23 692.0 0 23 28 26 692.0 0 26 29 29 692.0 0 29 30 30 692.0 0 30 31 24 692.0 0 24 32 35 692.0 0 35 33 75 692.0 0 75 34 32 692.0 0 32 35 34 692.0 0 34 36 26 692.0 0 26 37 40 692.0 0 40 38 37 692.0 0 37 39 42 692.0 0 42 40 363 692.0 0 363 41 34 692.0 0 34 42 54 692.0 0 54 43 41 692.0 0 41 44 25 692.0 0 25 45 45 692.0 0 45 46 258 692.0 0 258 47 259 692.0 0 259 48 267 692.0 0 267 49 25 692.0 0 25 50 27 692.0 0 27 51 35 692.0 0 35 52 39 692.0 0 39 53 25 692.0 0 25 54 28 692.0 0 28 55 30 692.0 0 30 56 23 692.0 0 23 57 32 692.0 0 32 58 31 692.0 0 31 59 45 692.0 0 45 60 46 692.0 0 46 61 45 692.0 0 45 62 32 692.0 0 32 63 44 692.0 0 44 64 33 692.0 0 33 65 35 692.0 0 35 66 47 692.0 0 47 67 43 692.0 0 43 68 29 692.0 0 29 69 48 692.0 0 48 70 42 692.0 0 42 71 27 692.0 0 27 72 40 692.0 0 40 73 33 692.0 0 33 74 25 692.0 0 25 75 25 692.0 0 25 76 40 692.0 0 40 77 60 692.0 0 60 78 61 692.0 0 61 79 134 692.0 0 134 80 307 692.0 0 307 81 458 692.0 0 458 82 138 692.0 0 138 83 172 692.0 0 172 84 129 692.0 0 129 85 69 692.0 0 69 86 76 692.0 0 76 87 94 692.0 0 94 88 75 692.0 0 75 89 111 692.0 0 111 90 76 692.0 0 76 91 108 692.0 0 108 92 127 692.0 0 127 93 175 692.0 0 175 94 2601 692.0 0 2601 95 4667 692.0 0 4667 96 4795 692.0 0 4795 97 73 692.0 0 73 98 202 692.0 0 202 99 33 692.0 0 33 100 41 692.0 0 41 101 29 692.0 0 29 102 29 692.0 0 29 103 26 692.0 0 26 104 34 692.0 0 34 105 32 692.0 0 32 106 36 692.0 0 36 107 43 692.0 0 43 108 33 692.0 0 33 109 33 692.0 0 33 110 61 692.0 0 61 111 38 692.0 0 38 112 50 692.0 0 50 113 46 692.0 0 46 114 45 692.0 0 45 115 32 692.0 0 32 116 30 692.0 0 30 117 33 692.0 0 33 118 75 692.0 0 75 119 42 692.0 0 42 120 62 692.0 0 62 121 38 692.0 0 38 122 47 692.0 0 47 123 50 692.0 0 50 124 201 692.0 0 201 125 476 692.0 0 476 126 231 692.0 0 231 127 40 692.0 0 40 128 32 692.0 0 32 129 40 692.0 0 40 130 69 692.0 0 69 131 99 692.0 0 99 132 82 692.0 0 82 133 208 692.0 0 208 134 46 692.0 0 46 135 46 692.0 0 46 136 70 692.0 0 70 137 211 692.0 0 211 138 259 692.0 0 259 139 317 692.0 0 317 140 55 692.0 0 55 141 84 692.0 0 84 142 66 692.0 0 66 143 62 692.0 0 62 144 103 692.0 0 103 145 49 692.0 0 49 146 83 692.0 0 83 147 72 692.0 0 72 148 39 692.0 0 39 149 131 692.0 0 131 150 17 692.0 0 17 151 18 692.0 0 18 152 13 692.0 0 13 153 13 692.0 0 13 154 18 692.0 0 18 155 21 692.0 0 21 156 29 692.0 0 29 157 29 692.0 0 29 158 94 692.0 0 94 159 31 692.0 0 31 160 70 692.0 0 70 161 22 692.0 0 22 162 22 692.0 0 22 163 20 692.0 0 20 164 20 692.0 0 20 165 17 692.0 0 17 166 28 692.0 0 28 167 21 692.0 0 21 168 15 692.0 0 15 169 18 692.0 0 18 170 16 692.0 0 16 171 17 692.0 0 17 172 18 692.0 0 18 173 16 692.0 0 16 174 15 692.0 0 15 175 19 692.0 0 19 176 21 692.0 0 21 177 26 692.0 0 26 178 14 692.0 0 14 179 25 692.0 0 25 180 23 692.0 0 23 181 13 692.0 0 13 182 33 692.0 0 33 183 19 692.0 0 19 184 20 692.0 0 20 185 22 692.0 0 22 186 34 692.0 0 34 187 28 692.0 0 28 188 32 692.0 0 32 189 16 692.0 0 16 190 23 692.0 0 23 191 16 692.0 0 16 192 42 692.0 0 42 193 32 692.0 0 32 194 28 692.0 0 28 195 25 692.0 0 25 196 15 692.0 0 15 197 18 692.0 0 18 198 20 692.0 0 20 199 16 692.0 0 16 200 15 692.0 0 15 201 14 692.0 0 14 202 20 692.0 0 20 203 29 692.0 0 29 204 32 692.0 0 32 205 215 692.0 0 215 206 52 692.0 0 52 207 125 692.0 0 125 208 18 692.0 0 18 209 13 692.0 0 13 210 18 692.0 0 18 211 21 692.0 0 21 212 15 692.0 0 15 213 16 692.0 0 16 214 15 692.0 0 15 215 17 692.0 0 17 216 17 692.0 0 17 217 26 692.0 0 26 218 17 692.0 0 17 219 21 692.0 0 21 220 28 692.0 0 28 221 20 692.0 0 20 222 15 692.0 0 15 223 32 692.0 0 32 224 18 692.0 0 18 225 28 692.0 0 28 226 16 692.0 0 16 227 16 692.0 0 16 228 26 692.0 0 26 229 30 692.0 0 30 230 19 692.0 0 19 231 16 692.0 0 16 232 23 692.0 0 23 233 25 692.0 0 25 234 27 692.0 0 27 235 12 692.0 0 12 236 17 692.0 0 17 237 20 692.0 0 20 238 35 692.0 0 35 239 24 692.0 0 24 240 32 692.0 0 32 241 27 692.0 0 27 242 29 692.0 0 29 243 30 692.0 0 30 244 19 692.0 0 19 245 23 692.0 0 23 246 27 692.0 0 27 247 26 692.0 0 26 248 17 692.0 0 17 249 24 692.0 0 24 250 25 692.0 0 25 251 27 692.0 0 27 252 18 692.0 0 18 253 29 692.0 0 29 254 35 692.0 0 35 255 28 692.0 0 28 256 22 692.0 0 22 257 29 692.0 0 29 258 19 692.0 0 19 259 19 692.0 0 19 260 24 692.0 0 24 261 32 692.0 0 32 262 51 692.0 0 51 263 73 692.0 0 73 264 32 692.0 0 32 265 25 692.0 0 25 266 28 692.0 0 28 267 29 692.0 0 29 268 18 692.0 0 18 269 26 692.0 0 26 270 54 692.0 0 54 271 42 692.0 0 42 272 65 692.0 0 65 273 27 692.0 0 27 274 38 692.0 0 38 275 40 692.0 0 40 276 38 692.0 0 38 277 37 692.0 0 37 278 31 692.0 0 31 279 30 692.0 0 30 280 24 692.0 0 24 281 56 692.0 0 56 282 57 692.0 0 57 283 58 692.0 0 58 284 224 692.0 0 224 285 228 692.0 0 228 286 107 692.0 0 107 287 34 692.0 0 34 288 61 692.0 0 61 289 35 692.0 0 35 290 40 692.0 0 40 291 49 692.0 0 49 292 43 692.0 0 43 293 30 692.0 0 30 294 25 692.0 0 25 295 34 692.0 0 34 296 24 692.0 0 24 297 39 692.0 0 39 298 47 692.0 0 47 299 66 692.0 0 66 300 62 692.0 0 62 301 42 692.0 0 42 Finished in 10.53 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 216,238 Reads with adapters: 110,441 (51.1%) Reads that were too short: 77 (0.0%) Reads written (passing filters): 110,364 (51.0%) Total basepairs processed: 61,302,552 bp Total written (filtered): 30,197,294 bp (49.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 110441 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 36 0.0 3 0 0 34 2 19 1008 0.0 3 0 979 20 4 5 20 108512 0.0 4 106657 1766 76 12 1 21 288 0.0 4 37 246 4 0 1 22 21 0.0 4 2 1 17 1 23 94 0.0 4 0 0 1 84 9 24 6 0.0 4 2 0 0 0 4 25 1 0.0 4 1 26 2 0.0 4 2 27 1 0.0 4 1 28 1 0.0 4 0 0 0 0 1 31 2 0.0 4 1 0 0 0 1 40 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 91 1 0.0 4 1 98 1 0.0 4 0 0 0 0 1 108 1 0.0 4 1 116 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 6 0.0 4 0 0 0 0 6 127 374 0.0 4 0 0 0 0 374 130 2 0.0 4 0 0 0 0 2 135 1 0.0 4 0 1 138 1 0.0 4 0 0 0 0 1 141 39 0.0 4 0 0 0 2 37 142 10 0.0 4 0 0 0 1 9 143 3 0.0 4 0 0 0 0 3 145 1 0.0 4 1 146 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 1 228 4 0.0 4 0 0 0 0 4 229 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.74 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 110,364 Reads with adapters: 1,238 (1.1%) Reads written (passing filters): 110,364 (100.0%) Total basepairs processed: 30,197,294 bp Total written (filtered): 30,173,460 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1238 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 12.7% C: 6.2% G: 76.5% T: 4.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 150 1724.4 0 150 4 19 431.1 0 19 5 2 107.8 0 2 8 1 1.7 0 1 9 10 0.4 0 0 10 10 25 0.1 1 1 24 11 861 0.0 1 8 853 12 87 0.0 1 1 86 13 3 0.0 1 0 3 15 6 0.0 1 0 6 24 4 0.0 1 4 35 2 0.0 1 1 1 52 1 0.0 1 1 64 1 0.0 1 1 107 1 0.0 1 0 1 152 28 0.0 1 3 25 158 2 0.0 1 2 191 5 0.0 1 0 5 193 1 0.0 1 0 1 210 22 0.0 1 15 7 211 1 0.0 1 0 1 225 4 0.0 1 4 233 2 0.0 1 2