This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCTGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.14 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 497,210 (17.5%) Reads that were too short: 21,956 (0.8%) Reads written (passing filters): 475,254 (16.8%) Total basepairs processed: 853,219,115 bp Total written (filtered): 89,115,646 bp (10.4%) === Adapter 1 === Sequence: CTCTGC; Type: regular 5'; Length: 6; Trimmed: 497210 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 531 692.0 0 531 7 11256 692.0 0 11256 8 452 692.0 0 452 9 7742 692.0 0 7742 10 37 692.0 0 37 11 36 692.0 0 36 12 23 692.0 0 23 13 29 692.0 0 29 14 122 692.0 0 122 15 65 692.0 0 65 16 69 692.0 0 69 17 22 692.0 0 22 18 14 692.0 0 14 19 26 692.0 0 26 20 13 692.0 0 13 21 36 692.0 0 36 22 42 692.0 0 42 23 159 692.0 0 159 24 184 692.0 0 184 25 44 692.0 0 44 26 17 692.0 0 17 27 40 692.0 0 40 28 33 692.0 0 33 29 35 692.0 0 35 30 33 692.0 0 33 31 48 692.0 0 48 32 40 692.0 0 40 33 94 692.0 0 94 34 79 692.0 0 79 35 155 692.0 0 155 36 67 692.0 0 67 37 61 692.0 0 61 38 58 692.0 0 58 39 59 692.0 0 59 40 93 692.0 0 93 41 57 692.0 0 57 42 48 692.0 0 48 43 49 692.0 0 49 44 38 692.0 0 38 45 44 692.0 0 44 46 92 692.0 0 92 47 39 692.0 0 39 48 68 692.0 0 68 49 47 692.0 0 47 50 61 692.0 0 61 51 66 692.0 0 66 52 87 692.0 0 87 53 100 692.0 0 100 54 49 692.0 0 49 55 41 692.0 0 41 56 82 692.0 0 82 57 153 692.0 0 153 58 241 692.0 0 241 59 336 692.0 0 336 60 85 692.0 0 85 61 157 692.0 0 157 62 56 692.0 0 56 63 268 692.0 0 268 64 57 692.0 0 57 65 60 692.0 0 60 66 49 692.0 0 49 67 76 692.0 0 76 68 32 692.0 0 32 69 60 692.0 0 60 70 57 692.0 0 57 71 80 692.0 0 80 72 77 692.0 0 77 73 40 692.0 0 40 74 71 692.0 0 71 75 56 692.0 0 56 76 87 692.0 0 87 77 755 692.0 0 755 78 226 692.0 0 226 79 85 692.0 0 85 80 130 692.0 0 130 81 82 692.0 0 82 82 66 692.0 0 66 83 112 692.0 0 112 84 180 692.0 0 180 85 91 692.0 0 91 86 217 692.0 0 217 87 618 692.0 0 618 88 509 692.0 0 509 89 170 692.0 0 170 90 133 692.0 0 133 91 163 692.0 0 163 92 128 692.0 0 128 93 86 692.0 0 86 94 166 692.0 0 166 95 101 692.0 0 101 96 295 692.0 0 295 97 877 692.0 0 877 98 248 692.0 0 248 99 565 692.0 0 565 100 459 692.0 0 459 101 249 692.0 0 249 102 556 692.0 0 556 103 186 692.0 0 186 104 163 692.0 0 163 105 181 692.0 0 181 106 668 692.0 0 668 107 712 692.0 0 712 108 1611 692.0 0 1611 109 1101 692.0 0 1101 110 1733 692.0 0 1733 111 3003 692.0 0 3003 112 7131 692.0 0 7131 113 20190 692.0 0 20190 114 213867 692.0 0 213867 115 49383 692.0 0 49383 116 85758 692.0 0 85758 117 434 692.0 0 434 118 161 692.0 0 161 119 60 692.0 0 60 120 57 692.0 0 57 121 41 692.0 0 41 122 44 692.0 0 44 123 54 692.0 0 54 124 71 692.0 0 71 125 67 692.0 0 67 126 172 692.0 0 172 127 135 692.0 0 135 128 225 692.0 0 225 129 215 692.0 0 215 130 121 692.0 0 121 131 104 692.0 0 104 132 66 692.0 0 66 133 59 692.0 0 59 134 63 692.0 0 63 135 95 692.0 0 95 136 139 692.0 0 139 137 93 692.0 0 93 138 146 692.0 0 146 139 218 692.0 0 218 140 785 692.0 0 785 141 719 692.0 0 719 142 1987 692.0 0 1987 143 482 692.0 0 482 144 439 692.0 0 439 145 301 692.0 0 301 146 373 692.0 0 373 147 4444 692.0 0 4444 148 6406 692.0 0 6406 149 3068 692.0 0 3068 150 422 692.0 0 422 151 516 692.0 0 516 152 901 692.0 0 901 153 16099 692.0 0 16099 154 5216 692.0 0 5216 155 4175 692.0 0 4175 156 893 692.0 0 893 157 390 692.0 0 390 158 304 692.0 0 304 159 44 692.0 0 44 160 122 692.0 0 122 161 95 692.0 0 95 162 154 692.0 0 154 163 124 692.0 0 124 164 103 692.0 0 103 165 111 692.0 0 111 166 58 692.0 0 58 167 123 692.0 0 123 168 398 692.0 0 398 169 358 692.0 0 358 170 647 692.0 0 647 171 111 692.0 0 111 172 47 692.0 0 47 173 29 692.0 0 29 174 25 692.0 0 25 175 40 692.0 0 40 176 130 692.0 0 130 177 54 692.0 0 54 178 65 692.0 0 65 179 51 692.0 0 51 180 59 692.0 0 59 181 164 692.0 0 164 182 109 692.0 0 109 183 188 692.0 0 188 184 549 692.0 0 549 185 382 692.0 0 382 186 877 692.0 0 877 187 258 692.0 0 258 188 137 692.0 0 137 189 57 692.0 0 57 190 61 692.0 0 61 191 81 692.0 0 81 192 89 692.0 0 89 193 60 692.0 0 60 194 55 692.0 0 55 195 34 692.0 0 34 196 58 692.0 0 58 197 81 692.0 0 81 198 88 692.0 0 88 199 273 692.0 0 273 200 60 692.0 0 60 201 51 692.0 0 51 202 168 692.0 0 168 203 193 692.0 0 193 204 129 692.0 0 129 205 75 692.0 0 75 206 51 692.0 0 51 207 45 692.0 0 45 208 35 692.0 0 35 209 37 692.0 0 37 210 34 692.0 0 34 211 49 692.0 0 49 212 31 692.0 0 31 213 57 692.0 0 57 214 61 692.0 0 61 215 68 692.0 0 68 216 682 692.0 0 682 217 218 692.0 0 218 218 240 692.0 0 240 219 57 692.0 0 57 220 36 692.0 0 36 221 42 692.0 0 42 222 47 692.0 0 47 223 44 692.0 0 44 224 39 692.0 0 39 225 47 692.0 0 47 226 98 692.0 0 98 227 66 692.0 0 66 228 77 692.0 0 77 229 47 692.0 0 47 230 51 692.0 0 51 231 55 692.0 0 55 232 75 692.0 0 75 233 125 692.0 0 125 234 183 692.0 0 183 235 357 692.0 0 357 236 143 692.0 0 143 237 184 692.0 0 184 238 93 692.0 0 93 239 79 692.0 0 79 240 39 692.0 0 39 241 51 692.0 0 51 242 51 692.0 0 51 243 51 692.0 0 51 244 102 692.0 0 102 245 77 692.0 0 77 246 171 692.0 0 171 247 91 692.0 0 91 248 90 692.0 0 90 249 284 692.0 0 284 250 117 692.0 0 117 251 236 692.0 0 236 252 83 692.0 0 83 253 93 692.0 0 93 254 99 692.0 0 99 255 104 692.0 0 104 256 98 692.0 0 98 257 96 692.0 0 96 258 62 692.0 0 62 259 66 692.0 0 66 260 71 692.0 0 71 261 85 692.0 0 85 262 78 692.0 0 78 263 139 692.0 0 139 264 102 692.0 0 102 265 97 692.0 0 97 266 63 692.0 0 63 267 114 692.0 0 114 268 127 692.0 0 127 269 199 692.0 0 199 270 236 692.0 0 236 271 533 692.0 0 533 272 175 692.0 0 175 273 91 692.0 0 91 274 204 692.0 0 204 275 172 692.0 0 172 276 226 692.0 0 226 277 153 692.0 0 153 278 167 692.0 0 167 279 737 692.0 0 737 280 1311 692.0 0 1311 281 2805 692.0 0 2805 282 1169 692.0 0 1169 283 2162 692.0 0 2162 284 180 692.0 0 180 285 308 692.0 0 308 286 143 692.0 0 143 287 204 692.0 0 204 288 86 692.0 0 86 289 176 692.0 0 176 290 440 692.0 0 440 291 348 692.0 0 348 292 442 692.0 0 442 293 244 692.0 0 244 294 259 692.0 0 259 295 314 692.0 0 314 296 414 692.0 0 414 297 392 692.0 0 392 298 377 692.0 0 377 299 132 692.0 0 132 300 48 692.0 0 48 301 54 692.0 0 54 Finished in 16.02 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 475,254 Reads with adapters: 12,072 (2.5%) Reads that were too short: 349 (0.1%) Reads written (passing filters): 11,723 (2.5%) Total basepairs processed: 89,115,646 bp Total written (filtered): 3,193,715 bp (3.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12072 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 4 0.0 3 0 0 3 1 16 93 0.0 3 0 73 13 7 17 11150 0.0 3 10544 508 74 24 18 41 0.0 3 8 27 2 4 19 17 0.0 3 0 1 9 7 20 92 0.0 3 1 0 0 91 21 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 1 0.0 3 1 31 2 0.0 3 0 0 0 2 32 4 0.0 3 0 0 1 3 33 4 0.0 3 0 0 0 4 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 3 0.0 3 0 0 0 3 42 2 0.0 3 0 0 1 1 45 5 0.0 3 0 0 0 5 46 1 0.0 3 0 1 47 3 0.0 3 1 1 1 48 5 0.0 3 0 0 0 5 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 84 0.0 3 0 0 0 84 52 1 0.0 3 0 0 0 1 54 8 0.0 3 0 1 0 7 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 1 57 1 0.0 3 0 0 0 1 58 13 0.0 3 0 0 1 12 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 1 61 3 0.0 3 0 0 1 2 62 2 0.0 3 0 0 0 2 63 2 0.0 3 0 0 0 2 64 4 0.0 3 0 0 0 4 65 6 0.0 3 0 0 0 6 66 3 0.0 3 1 1 0 1 68 3 0.0 3 0 0 1 2 69 2 0.0 3 0 0 1 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 75 4 0.0 3 0 0 0 4 76 2 0.0 3 0 0 0 2 77 4 0.0 3 0 0 0 4 79 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 0 2 82 2 0.0 3 0 0 0 2 85 111 0.0 3 0 0 2 109 86 3 0.0 3 0 0 0 3 87 4 0.0 3 0 0 0 4 88 10 0.0 3 0 0 0 10 89 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 3 0.0 3 0 0 1 2 97 2 0.0 3 0 1 0 1 98 2 0.0 3 0 0 0 2 99 3 0.0 3 1 0 0 2 100 1 0.0 3 1 101 3 0.0 3 1 1 0 1 102 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 105 3 0.0 3 0 0 0 3 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 110 7 0.0 3 0 0 0 7 111 2 0.0 3 0 0 0 2 114 4 0.0 3 0 0 1 3 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 130 6 0.0 3 0 1 0 5 131 7 0.0 3 0 0 0 7 133 1 0.0 3 0 0 0 1 134 11 0.0 3 0 0 3 8 135 7 0.0 3 0 0 0 7 136 8 0.0 3 0 0 0 8 137 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 139 2 0.0 3 1 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 148 4 0.0 3 0 0 0 4 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 3 0.0 3 0 0 3 152 6 0.0 3 0 0 0 6 153 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 1 1 168 1 0.0 3 0 0 0 1 172 49 0.0 3 0 0 0 49 173 9 0.0 3 0 0 0 9 174 79 0.0 3 0 0 1 78 175 35 0.0 3 0 0 0 35 176 1 0.0 3 0 0 0 1 177 4 0.0 3 0 0 0 4 179 1 0.0 3 0 0 0 1 180 3 0.0 3 0 0 0 3 181 5 0.0 3 0 0 0 5 183 1 0.0 3 0 0 0 1 184 3 0.0 3 0 0 0 3 185 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 1 1 187 1 0.0 3 0 0 0 1 189 3 0.0 3 0 0 0 3 191 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 194 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 225 2 0.0 3 0 0 0 2 226 3 0.0 3 0 0 1 2 235 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.41 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 11,723 Reads with adapters: 120 (1.0%) Reads written (passing filters): 11,723 (100.0%) Total basepairs processed: 3,193,715 bp Total written (filtered): 3,193,346 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 120 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.3% C: 35.0% G: 27.5% T: 4.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 114 183.2 0 114 4 3 45.8 0 3 5 3 11.4 0 3