This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.08 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 179,087 (6.3%) Reads that were too short: 43,790 (1.5%) Reads written (passing filters): 135,297 (4.8%) Total basepairs processed: 853,219,115 bp Total written (filtered): 37,989,504 bp (4.5%) === Adapter 1 === Sequence: TGTGGC; Type: regular 5'; Length: 6; Trimmed: 179087 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2804 692.0 0 2804 7 48343 692.0 0 48343 8 2007 692.0 0 2007 9 67070 692.0 0 67070 10 71 692.0 0 71 11 43 692.0 0 43 12 46 692.0 0 46 13 19 692.0 0 19 14 24 692.0 0 24 15 53 692.0 0 53 16 29 692.0 0 29 17 51 692.0 0 51 18 24 692.0 0 24 19 56 692.0 0 56 20 24 692.0 0 24 21 36 692.0 0 36 22 25 692.0 0 25 23 27 692.0 0 27 24 25 692.0 0 25 25 26 692.0 0 26 26 19 692.0 0 19 27 18 692.0 0 18 28 24 692.0 0 24 29 49 692.0 0 49 30 133 692.0 0 133 31 41 692.0 0 41 32 53 692.0 0 53 33 46 692.0 0 46 34 32 692.0 0 32 35 25 692.0 0 25 36 99 692.0 0 99 37 30 692.0 0 30 38 36 692.0 0 36 39 32 692.0 0 32 40 43 692.0 0 43 41 27 692.0 0 27 42 31 692.0 0 31 43 41 692.0 0 41 44 25 692.0 0 25 45 31 692.0 0 31 46 22 692.0 0 22 47 65 692.0 0 65 48 46 692.0 0 46 49 69 692.0 0 69 50 52 692.0 0 52 51 62 692.0 0 62 52 32 692.0 0 32 53 47 692.0 0 47 54 37 692.0 0 37 55 43 692.0 0 43 56 45 692.0 0 45 57 358 692.0 0 358 58 48 692.0 0 48 59 81 692.0 0 81 60 39 692.0 0 39 61 43 692.0 0 43 62 39 692.0 0 39 63 58 692.0 0 58 64 70 692.0 0 70 65 29 692.0 0 29 66 48 692.0 0 48 67 27 692.0 0 27 68 104 692.0 0 104 69 44 692.0 0 44 70 58 692.0 0 58 71 98 692.0 0 98 72 202 692.0 0 202 73 161 692.0 0 161 74 24 692.0 0 24 75 56 692.0 0 56 76 20 692.0 0 20 77 55 692.0 0 55 78 56 692.0 0 56 79 40 692.0 0 40 80 34 692.0 0 34 81 44 692.0 0 44 82 39 692.0 0 39 83 67 692.0 0 67 84 50 692.0 0 50 85 57 692.0 0 57 86 57 692.0 0 57 87 58 692.0 0 58 88 41 692.0 0 41 89 35 692.0 0 35 90 66 692.0 0 66 91 43 692.0 0 43 92 46 692.0 0 46 93 72 692.0 0 72 94 73 692.0 0 73 95 66 692.0 0 66 96 37 692.0 0 37 97 33 692.0 0 33 98 52 692.0 0 52 99 34 692.0 0 34 100 24 692.0 0 24 101 32 692.0 0 32 102 41 692.0 0 41 103 32 692.0 0 32 104 67 692.0 0 67 105 27 692.0 0 27 106 49 692.0 0 49 107 32 692.0 0 32 108 33 692.0 0 33 109 36 692.0 0 36 110 34 692.0 0 34 111 56 692.0 0 56 112 36 692.0 0 36 113 30 692.0 0 30 114 30 692.0 0 30 115 32 692.0 0 32 116 41 692.0 0 41 117 82 692.0 0 82 118 48 692.0 0 48 119 50 692.0 0 50 120 58 692.0 0 58 121 38 692.0 0 38 122 41 692.0 0 41 123 248 692.0 0 248 124 86 692.0 0 86 125 448 692.0 0 448 126 114 692.0 0 114 127 61 692.0 0 61 128 100 692.0 0 100 129 96 692.0 0 96 130 1376 692.0 0 1376 131 368 692.0 0 368 132 984 692.0 0 984 133 254 692.0 0 254 134 300 692.0 0 300 135 135 692.0 0 135 136 82 692.0 0 82 137 358 692.0 0 358 138 847 692.0 0 847 139 167 692.0 0 167 140 37 692.0 0 37 141 39 692.0 0 39 142 57 692.0 0 57 143 65 692.0 0 65 144 34 692.0 0 34 145 21 692.0 0 21 146 37 692.0 0 37 147 32 692.0 0 32 148 26 692.0 0 26 149 43 692.0 0 43 150 64 692.0 0 64 151 124 692.0 0 124 152 58 692.0 0 58 153 61 692.0 0 61 154 48 692.0 0 48 155 36 692.0 0 36 156 33 692.0 0 33 157 70 692.0 0 70 158 37 692.0 0 37 159 34 692.0 0 34 160 19 692.0 0 19 161 29 692.0 0 29 162 29 692.0 0 29 163 24 692.0 0 24 164 23 692.0 0 23 165 36 692.0 0 36 166 43 692.0 0 43 167 55 692.0 0 55 168 37 692.0 0 37 169 28 692.0 0 28 170 29 692.0 0 29 171 24 692.0 0 24 172 21 692.0 0 21 173 27 692.0 0 27 174 41 692.0 0 41 175 51 692.0 0 51 176 32 692.0 0 32 177 106 692.0 0 106 178 91 692.0 0 91 179 602 692.0 0 602 180 44 692.0 0 44 181 26 692.0 0 26 182 36 692.0 0 36 183 28 692.0 0 28 184 34 692.0 0 34 185 40 692.0 0 40 186 76 692.0 0 76 187 139 692.0 0 139 188 91 692.0 0 91 189 97 692.0 0 97 190 49 692.0 0 49 191 22 692.0 0 22 192 27 692.0 0 27 193 29 692.0 0 29 194 25 692.0 0 25 195 34 692.0 0 34 196 47 692.0 0 47 197 59 692.0 0 59 198 49 692.0 0 49 199 55 692.0 0 55 200 48 692.0 0 48 201 46 692.0 0 46 202 37 692.0 0 37 203 73 692.0 0 73 204 144 692.0 0 144 205 64 692.0 0 64 206 53 692.0 0 53 207 34 692.0 0 34 208 38 692.0 0 38 209 48 692.0 0 48 210 54 692.0 0 54 211 96 692.0 0 96 212 823 692.0 0 823 213 410 692.0 0 410 214 1181 692.0 0 1181 215 63 692.0 0 63 216 173 692.0 0 173 217 93 692.0 0 93 218 213 692.0 0 213 219 39 692.0 0 39 220 54 692.0 0 54 221 32 692.0 0 32 222 37 692.0 0 37 223 54 692.0 0 54 224 49 692.0 0 49 225 34 692.0 0 34 226 39 692.0 0 39 227 35 692.0 0 35 228 64 692.0 0 64 229 52 692.0 0 52 230 58 692.0 0 58 231 73 692.0 0 73 232 63 692.0 0 63 233 98 692.0 0 98 234 87 692.0 0 87 235 173 692.0 0 173 236 423 692.0 0 423 237 517 692.0 0 517 238 706 692.0 0 706 239 176 692.0 0 176 240 65 692.0 0 65 241 272 692.0 0 272 242 169 692.0 0 169 243 1263 692.0 0 1263 244 705 692.0 0 705 245 941 692.0 0 941 246 596 692.0 0 596 247 172 692.0 0 172 248 103 692.0 0 103 249 193 692.0 0 193 250 170 692.0 0 170 251 233 692.0 0 233 252 148 692.0 0 148 253 65 692.0 0 65 254 131 692.0 0 131 255 203 692.0 0 203 256 218 692.0 0 218 257 405 692.0 0 405 258 355 692.0 0 355 259 272 692.0 0 272 260 460 692.0 0 460 261 449 692.0 0 449 262 536 692.0 0 536 263 302 692.0 0 302 264 94 692.0 0 94 265 504 692.0 0 504 266 110 692.0 0 110 267 104 692.0 0 104 268 121 692.0 0 121 269 66 692.0 0 66 270 77 692.0 0 77 271 144 692.0 0 144 272 301 692.0 0 301 273 456 692.0 0 456 274 170 692.0 0 170 275 304 692.0 0 304 276 326 692.0 0 326 277 299 692.0 0 299 278 124 692.0 0 124 279 217 692.0 0 217 280 345 692.0 0 345 281 415 692.0 0 415 282 271 692.0 0 271 283 276 692.0 0 276 284 394 692.0 0 394 285 565 692.0 0 565 286 10554 692.0 0 10554 287 5132 692.0 0 5132 288 4784 692.0 0 4784 289 94 692.0 0 94 290 87 692.0 0 87 291 86 692.0 0 86 292 68 692.0 0 68 293 84 692.0 0 84 294 98 692.0 0 98 295 124 692.0 0 124 296 327 692.0 0 327 297 578 692.0 0 578 298 621 692.0 0 621 299 361 692.0 0 361 300 165 692.0 0 165 301 58 692.0 0 58 Finished in 6.21 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 135,297 Reads with adapters: 70,074 (51.8%) Reads that were too short: 119 (0.1%) Reads written (passing filters): 69,955 (51.7%) Total basepairs processed: 37,989,504 bp Total written (filtered): 19,215,865 bp (50.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 70074 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 35 0.0 3 0 0 27 8 16 558 0.0 3 0 463 70 25 17 68517 0.0 3 64000 3658 692 167 18 179 0.0 3 7 135 28 9 19 55 0.0 3 1 0 31 23 20 440 0.0 3 1 0 0 439 21 1 0.0 3 0 1 27 1 0.0 3 1 29 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 32 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 39 2 0.0 3 1 0 0 1 41 12 0.0 3 0 0 0 12 42 2 0.0 3 0 0 1 1 43 1 0.0 3 0 0 0 1 44 4 0.0 3 0 0 0 4 46 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 61 0.0 3 0 0 1 60 62 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 64 12 0.0 3 0 0 0 12 65 1 0.0 3 0 0 0 1 66 7 0.0 3 0 0 4 3 68 2 0.0 3 0 0 0 2 72 1 0.0 3 0 1 74 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 89 2 0.0 3 0 0 2 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 3 0.0 3 0 0 0 3 94 1 0.0 3 0 0 0 1 97 3 0.0 3 0 0 0 3 98 5 0.0 3 0 0 0 5 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 1 107 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 3 0.0 3 1 0 0 2 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 151 2 0.0 3 1 1 153 3 0.0 3 0 0 0 3 154 3 0.0 3 0 0 0 3 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 163 7 0.0 3 0 0 1 6 164 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 178 2 0.0 3 0 0 0 2 182 1 0.0 3 0 0 0 1 183 4 0.0 3 0 0 0 4 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 189 2 0.0 3 0 0 0 2 192 16 0.0 3 0 0 0 16 193 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 195 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 3 0.0 3 0 0 0 3 205 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 2 0.0 3 0 0 0 2 214 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 248 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 277 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 5 0.0 3 0 0 0 5 281 11 0.0 3 0 0 0 11 282 4 0.0 3 0 0 0 4 286 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.48 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 69,955 Reads with adapters: 1,088 (1.6%) Reads written (passing filters): 69,955 (100.0%) Total basepairs processed: 19,215,865 bp Total written (filtered): 19,212,583 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1088 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 37.6% C: 11.4% G: 48.3% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1072 1093.0 0 1072 4 14 273.3 0 14 5 2 68.3 0 2