This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 72.44 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 186,112 (6.6%) Reads that were too short: 48,207 (1.7%) Reads written (passing filters): 137,905 (4.9%) Total basepairs processed: 853,219,115 bp Total written (filtered): 39,016,275 bp (4.6%) === Adapter 1 === Sequence: TGTGGC; Type: regular 5'; Length: 6; Trimmed: 186112 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3165 692.0 0 3165 7 68536 692.0 0 68536 8 1415 692.0 0 1415 9 51376 692.0 0 51376 10 75 692.0 0 75 11 37 692.0 0 37 12 48 692.0 0 48 13 35 692.0 0 35 14 40 692.0 0 40 15 42 692.0 0 42 16 35 692.0 0 35 17 26 692.0 0 26 18 45 692.0 0 45 19 46 692.0 0 46 20 21 692.0 0 21 21 23 692.0 0 23 22 33 692.0 0 33 23 32 692.0 0 32 24 26 692.0 0 26 25 30 692.0 0 30 26 30 692.0 0 30 27 25 692.0 0 25 28 39 692.0 0 39 29 31 692.0 0 31 30 37 692.0 0 37 31 27 692.0 0 27 32 50 692.0 0 50 33 40 692.0 0 40 34 56 692.0 0 56 35 36 692.0 0 36 36 109 692.0 0 109 37 53 692.0 0 53 38 43 692.0 0 43 39 31 692.0 0 31 40 46 692.0 0 46 41 36 692.0 0 36 42 42 692.0 0 42 43 41 692.0 0 41 44 37 692.0 0 37 45 38 692.0 0 38 46 32 692.0 0 32 47 55 692.0 0 55 48 35 692.0 0 35 49 64 692.0 0 64 50 60 692.0 0 60 51 51 692.0 0 51 52 57 692.0 0 57 53 51 692.0 0 51 54 59 692.0 0 59 55 39 692.0 0 39 56 43 692.0 0 43 57 411 692.0 0 411 58 51 692.0 0 51 59 48 692.0 0 48 60 47 692.0 0 47 61 48 692.0 0 48 62 47 692.0 0 47 63 71 692.0 0 71 64 53 692.0 0 53 65 37 692.0 0 37 66 45 692.0 0 45 67 38 692.0 0 38 68 92 692.0 0 92 69 55 692.0 0 55 70 25 692.0 0 25 71 101 692.0 0 101 72 204 692.0 0 204 73 140 692.0 0 140 74 22 692.0 0 22 75 50 692.0 0 50 76 23 692.0 0 23 77 46 692.0 0 46 78 45 692.0 0 45 79 37 692.0 0 37 80 36 692.0 0 36 81 51 692.0 0 51 82 35 692.0 0 35 83 70 692.0 0 70 84 31 692.0 0 31 85 43 692.0 0 43 86 55 692.0 0 55 87 17 692.0 0 17 88 48 692.0 0 48 89 34 692.0 0 34 90 51 692.0 0 51 91 48 692.0 0 48 92 51 692.0 0 51 93 58 692.0 0 58 94 56 692.0 0 56 95 51 692.0 0 51 96 27 692.0 0 27 97 32 692.0 0 32 98 38 692.0 0 38 99 24 692.0 0 24 100 30 692.0 0 30 101 18 692.0 0 18 102 31 692.0 0 31 103 35 692.0 0 35 104 30 692.0 0 30 105 23 692.0 0 23 106 37 692.0 0 37 107 20 692.0 0 20 108 26 692.0 0 26 109 40 692.0 0 40 110 28 692.0 0 28 111 37 692.0 0 37 112 24 692.0 0 24 113 22 692.0 0 22 114 26 692.0 0 26 115 30 692.0 0 30 116 39 692.0 0 39 117 71 692.0 0 71 118 41 692.0 0 41 119 37 692.0 0 37 120 51 692.0 0 51 121 27 692.0 0 27 122 25 692.0 0 25 123 240 692.0 0 240 124 70 692.0 0 70 125 474 692.0 0 474 126 88 692.0 0 88 127 59 692.0 0 59 128 96 692.0 0 96 129 75 692.0 0 75 130 1294 692.0 0 1294 131 232 692.0 0 232 132 928 692.0 0 928 133 179 692.0 0 179 134 256 692.0 0 256 135 129 692.0 0 129 136 48 692.0 0 48 137 249 692.0 0 249 138 738 692.0 0 738 139 201 692.0 0 201 140 23 692.0 0 23 141 19 692.0 0 19 142 24 692.0 0 24 143 38 692.0 0 38 144 22 692.0 0 22 145 19 692.0 0 19 146 14 692.0 0 14 147 23 692.0 0 23 148 25 692.0 0 25 149 38 692.0 0 38 150 35 692.0 0 35 151 57 692.0 0 57 152 52 692.0 0 52 153 42 692.0 0 42 154 43 692.0 0 43 155 26 692.0 0 26 156 18 692.0 0 18 157 61 692.0 0 61 158 23 692.0 0 23 159 46 692.0 0 46 160 25 692.0 0 25 161 15 692.0 0 15 162 22 692.0 0 22 163 19 692.0 0 19 164 31 692.0 0 31 165 26 692.0 0 26 166 18 692.0 0 18 167 45 692.0 0 45 168 28 692.0 0 28 169 19 692.0 0 19 170 18 692.0 0 18 171 13 692.0 0 13 172 20 692.0 0 20 173 34 692.0 0 34 174 29 692.0 0 29 175 49 692.0 0 49 176 25 692.0 0 25 177 112 692.0 0 112 178 94 692.0 0 94 179 783 692.0 0 783 180 49 692.0 0 49 181 16 692.0 0 16 182 23 692.0 0 23 183 23 692.0 0 23 184 31 692.0 0 31 185 21 692.0 0 21 186 32 692.0 0 32 187 38 692.0 0 38 188 41 692.0 0 41 189 36 692.0 0 36 190 39 692.0 0 39 191 23 692.0 0 23 192 37 692.0 0 37 193 23 692.0 0 23 194 24 692.0 0 24 195 19 692.0 0 19 196 25 692.0 0 25 197 29 692.0 0 29 198 41 692.0 0 41 199 34 692.0 0 34 200 32 692.0 0 32 201 21 692.0 0 21 202 22 692.0 0 22 203 27 692.0 0 27 204 54 692.0 0 54 205 20 692.0 0 20 206 25 692.0 0 25 207 22 692.0 0 22 208 21 692.0 0 21 209 25 692.0 0 25 210 28 692.0 0 28 211 45 692.0 0 45 212 605 692.0 0 605 213 164 692.0 0 164 214 476 692.0 0 476 215 31 692.0 0 31 216 33 692.0 0 33 217 47 692.0 0 47 218 113 692.0 0 113 219 29 692.0 0 29 220 44 692.0 0 44 221 27 692.0 0 27 222 26 692.0 0 26 223 25 692.0 0 25 224 12 692.0 0 12 225 24 692.0 0 24 226 24 692.0 0 24 227 31 692.0 0 31 228 28 692.0 0 28 229 29 692.0 0 29 230 24 692.0 0 24 231 25 692.0 0 25 232 35 692.0 0 35 233 32 692.0 0 32 234 43 692.0 0 43 235 110 692.0 0 110 236 116 692.0 0 116 237 225 692.0 0 225 238 164 692.0 0 164 239 54 692.0 0 54 240 34 692.0 0 34 241 43 692.0 0 43 242 46 692.0 0 46 243 264 692.0 0 264 244 249 692.0 0 249 245 219 692.0 0 219 246 301 692.0 0 301 247 88 692.0 0 88 248 62 692.0 0 62 249 78 692.0 0 78 250 73 692.0 0 73 251 79 692.0 0 79 252 43 692.0 0 43 253 35 692.0 0 35 254 40 692.0 0 40 255 71 692.0 0 71 256 80 692.0 0 80 257 208 692.0 0 208 258 156 692.0 0 156 259 120 692.0 0 120 260 537 692.0 0 537 261 465 692.0 0 465 262 730 692.0 0 730 263 358 692.0 0 358 264 42 692.0 0 42 265 570 692.0 0 570 266 63 692.0 0 63 267 60 692.0 0 60 268 55 692.0 0 55 269 49 692.0 0 49 270 57 692.0 0 57 271 138 692.0 0 138 272 363 692.0 0 363 273 660 692.0 0 660 274 161 692.0 0 161 275 201 692.0 0 201 276 342 692.0 0 342 277 172 692.0 0 172 278 129 692.0 0 129 279 303 692.0 0 303 280 421 692.0 0 421 281 426 692.0 0 426 282 312 692.0 0 312 283 343 692.0 0 343 284 411 692.0 0 411 285 579 692.0 0 579 286 16837 692.0 0 16837 287 8997 692.0 0 8997 288 7970 692.0 0 7970 289 45 692.0 0 45 290 74 692.0 0 74 291 49 692.0 0 49 292 40 692.0 0 40 293 57 692.0 0 57 294 46 692.0 0 46 295 71 692.0 0 71 296 125 692.0 0 125 297 227 692.0 0 227 298 270 692.0 0 270 299 143 692.0 0 143 300 113 692.0 0 113 301 22 692.0 0 22 Finished in 6.82 s (49 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 137,905 Reads with adapters: 70,826 (51.4%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 70,806 (51.3%) Total basepairs processed: 39,016,275 bp Total written (filtered): 19,384,744 bp (49.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 70826 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 1 0.0 3 0 0 0 1 18 22 0.0 3 0 0 22 19 682 0.0 3 0 666 15 0 1 20 69716 0.0 4 68507 1164 41 3 1 21 147 0.0 4 22 122 3 22 13 0.0 4 1 0 11 1 23 48 0.0 4 0 0 0 46 2 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 0 0 1 27 1 0.0 4 0 1 35 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 64 1 0.0 4 1 65 1 0.0 4 0 1 69 2 0.0 4 0 2 70 1 0.0 4 1 71 1 0.0 4 1 84 1 0.0 4 1 86 1 0.0 4 0 0 0 0 1 90 1 0.0 4 1 92 1 0.0 4 0 0 0 0 1 98 2 0.0 4 0 0 0 0 2 99 1 0.0 4 0 0 0 0 1 104 1 0.0 4 1 116 1 0.0 4 1 125 1 0.0 4 0 0 0 0 1 126 3 0.0 4 1 0 0 0 2 127 156 0.0 4 0 0 0 0 156 128 2 0.0 4 0 0 0 0 2 140 1 0.0 4 0 0 0 0 1 143 1 0.0 4 1 153 2 0.0 4 0 0 0 0 2 166 1 0.0 4 1 213 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 288 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.42 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 70,806 Reads with adapters: 551 (0.8%) Reads written (passing filters): 70,806 (100.0%) Total basepairs processed: 19,384,744 bp Total written (filtered): 19,376,856 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 551 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 22.1% C: 8.2% G: 66.2% T: 3.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 93 1106.3 0 93 4 9 276.6 0 9 5 3 69.1 0 3 8 1 1.1 0 1 9 3 0.3 0 1 2 10 11 0.1 1 0 11 11 309 0.0 1 1 308 12 55 0.0 1 3 52 13 5 0.0 1 0 5 15 2 0.0 1 0 2 20 30 0.0 1 21 9 21 2 0.0 1 1 1 23 3 0.0 1 3 24 11 0.0 1 8 3 101 1 0.0 1 1 113 1 0.0 1 1 115 1 0.0 1 1 132 1 0.0 1 1 152 4 0.0 1 0 4 184 1 0.0 1 1 191 1 0.0 1 0 1 210 4 0.0 1 1 3