This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.45 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 43,554 (1.5%) Reads that were too short: 19,116 (0.7%) Reads written (passing filters): 24,438 (0.9%) Total basepairs processed: 853,219,115 bp Total written (filtered): 4,841,700 bp (0.6%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 43554 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 74 692.0 0 74 7 142 692.0 0 142 8 1832 692.0 0 1832 9 1545 692.0 0 1545 10 42 692.0 0 42 11 34 692.0 0 34 12 35 692.0 0 35 13 45 692.0 0 45 14 44 692.0 0 44 15 43 692.0 0 43 16 52 692.0 0 52 17 49 692.0 0 49 18 37 692.0 0 37 19 36 692.0 0 36 20 34 692.0 0 34 21 45 692.0 0 45 22 41 692.0 0 41 23 51 692.0 0 51 24 40 692.0 0 40 25 42 692.0 0 42 26 43 692.0 0 43 27 41 692.0 0 41 28 33 692.0 0 33 29 39 692.0 0 39 30 41 692.0 0 41 31 42 692.0 0 42 32 59 692.0 0 59 33 43 692.0 0 43 34 45 692.0 0 45 35 52 692.0 0 52 36 38 692.0 0 38 37 48 692.0 0 48 38 135 692.0 0 135 39 50 692.0 0 50 40 80 692.0 0 80 41 61 692.0 0 61 42 44 692.0 0 44 43 51 692.0 0 51 44 35 692.0 0 35 45 54 692.0 0 54 46 34 692.0 0 34 47 50 692.0 0 50 48 29 692.0 0 29 49 54 692.0 0 54 50 40 692.0 0 40 51 57 692.0 0 57 52 56 692.0 0 56 53 77 692.0 0 77 54 44 692.0 0 44 55 51 692.0 0 51 56 35 692.0 0 35 57 42 692.0 0 42 58 42 692.0 0 42 59 36 692.0 0 36 60 55 692.0 0 55 61 60 692.0 0 60 62 39 692.0 0 39 63 51 692.0 0 51 64 60 692.0 0 60 65 61 692.0 0 61 66 69 692.0 0 69 67 104 692.0 0 104 68 75 692.0 0 75 69 50 692.0 0 50 70 51 692.0 0 51 71 60 692.0 0 60 72 42 692.0 0 42 73 60 692.0 0 60 74 83 692.0 0 83 75 77 692.0 0 77 76 88 692.0 0 88 77 51 692.0 0 51 78 66 692.0 0 66 79 64 692.0 0 64 80 61 692.0 0 61 81 54 692.0 0 54 82 87 692.0 0 87 83 77 692.0 0 77 84 62 692.0 0 62 85 40 692.0 0 40 86 66 692.0 0 66 87 64 692.0 0 64 88 65 692.0 0 65 89 77 692.0 0 77 90 72 692.0 0 72 91 132 692.0 0 132 92 66 692.0 0 66 93 55 692.0 0 55 94 94 692.0 0 94 95 210 692.0 0 210 96 105 692.0 0 105 97 968 692.0 0 968 98 766 692.0 0 766 99 695 692.0 0 695 100 1082 692.0 0 1082 101 77 692.0 0 77 102 41 692.0 0 41 103 48 692.0 0 48 104 53 692.0 0 53 105 58 692.0 0 58 106 63 692.0 0 63 107 86 692.0 0 86 108 130 692.0 0 130 109 151 692.0 0 151 110 114 692.0 0 114 111 586 692.0 0 586 112 1547 692.0 0 1547 113 532 692.0 0 532 114 55 692.0 0 55 115 52 692.0 0 52 116 54 692.0 0 54 117 72 692.0 0 72 118 35 692.0 0 35 119 56 692.0 0 56 120 54 692.0 0 54 121 34 692.0 0 34 122 52 692.0 0 52 123 55 692.0 0 55 124 41 692.0 0 41 125 37 692.0 0 37 126 67 692.0 0 67 127 52 692.0 0 52 128 63 692.0 0 63 129 100 692.0 0 100 130 103 692.0 0 103 131 82 692.0 0 82 132 79 692.0 0 79 133 68 692.0 0 68 134 207 692.0 0 207 135 81 692.0 0 81 136 98 692.0 0 98 137 73 692.0 0 73 138 78 692.0 0 78 139 35 692.0 0 35 140 103 692.0 0 103 141 104 692.0 0 104 142 56 692.0 0 56 143 58 692.0 0 58 144 64 692.0 0 64 145 64 692.0 0 64 146 118 692.0 0 118 147 84 692.0 0 84 148 100 692.0 0 100 149 84 692.0 0 84 150 65 692.0 0 65 151 71 692.0 0 71 152 64 692.0 0 64 153 63 692.0 0 63 154 158 692.0 0 158 155 207 692.0 0 207 156 481 692.0 0 481 157 178 692.0 0 178 158 54 692.0 0 54 159 40 692.0 0 40 160 61 692.0 0 61 161 56 692.0 0 56 162 55 692.0 0 55 163 58 692.0 0 58 164 71 692.0 0 71 165 65 692.0 0 65 166 302 692.0 0 302 167 232 692.0 0 232 168 253 692.0 0 253 169 43 692.0 0 43 170 60 692.0 0 60 171 45 692.0 0 45 172 45 692.0 0 45 173 36 692.0 0 36 174 61 692.0 0 61 175 57 692.0 0 57 176 51 692.0 0 51 177 61 692.0 0 61 178 55 692.0 0 55 179 64 692.0 0 64 180 64 692.0 0 64 181 93 692.0 0 93 182 66 692.0 0 66 183 49 692.0 0 49 184 61 692.0 0 61 185 76 692.0 0 76 186 92 692.0 0 92 187 64 692.0 0 64 188 62 692.0 0 62 189 61 692.0 0 61 190 67 692.0 0 67 191 59 692.0 0 59 192 91 692.0 0 91 193 297 692.0 0 297 194 380 692.0 0 380 195 434 692.0 0 434 196 220 692.0 0 220 197 164 692.0 0 164 198 106 692.0 0 106 199 129 692.0 0 129 200 256 692.0 0 256 201 223 692.0 0 223 202 212 692.0 0 212 203 62 692.0 0 62 204 62 692.0 0 62 205 61 692.0 0 61 206 73 692.0 0 73 207 116 692.0 0 116 208 107 692.0 0 107 209 77 692.0 0 77 210 88 692.0 0 88 211 65 692.0 0 65 212 59 692.0 0 59 213 70 692.0 0 70 214 85 692.0 0 85 215 83 692.0 0 83 216 135 692.0 0 135 217 140 692.0 0 140 218 71 692.0 0 71 219 93 692.0 0 93 220 634 692.0 0 634 221 535 692.0 0 535 222 1490 692.0 0 1490 223 248 692.0 0 248 224 69 692.0 0 69 225 58 692.0 0 58 226 58 692.0 0 58 227 61 692.0 0 61 228 69 692.0 0 69 229 69 692.0 0 69 230 53 692.0 0 53 231 66 692.0 0 66 232 71 692.0 0 71 233 63 692.0 0 63 234 51 692.0 0 51 235 65 692.0 0 65 236 90 692.0 0 90 237 129 692.0 0 129 238 171 692.0 0 171 239 269 692.0 0 269 240 186 692.0 0 186 241 188 692.0 0 188 242 232 692.0 0 232 243 524 692.0 0 524 244 243 692.0 0 243 245 351 692.0 0 351 246 108 692.0 0 108 247 68 692.0 0 68 248 84 692.0 0 84 249 106 692.0 0 106 250 106 692.0 0 106 251 126 692.0 0 126 252 181 692.0 0 181 253 182 692.0 0 182 254 541 692.0 0 541 255 263 692.0 0 263 256 719 692.0 0 719 257 102 692.0 0 102 258 109 692.0 0 109 259 175 692.0 0 175 260 104 692.0 0 104 261 148 692.0 0 148 262 740 692.0 0 740 263 916 692.0 0 916 264 1768 692.0 0 1768 265 109 692.0 0 109 266 72 692.0 0 72 267 76 692.0 0 76 268 84 692.0 0 84 269 143 692.0 0 143 270 139 692.0 0 139 271 114 692.0 0 114 272 97 692.0 0 97 273 110 692.0 0 110 274 172 692.0 0 172 275 144 692.0 0 144 276 202 692.0 0 202 277 81 692.0 0 81 278 90 692.0 0 90 279 133 692.0 0 133 280 198 692.0 0 198 281 238 692.0 0 238 282 160 692.0 0 160 283 122 692.0 0 122 284 88 692.0 0 88 285 84 692.0 0 84 286 67 692.0 0 67 287 74 692.0 0 74 288 111 692.0 0 111 289 203 692.0 0 203 290 144 692.0 0 144 291 154 692.0 0 154 292 146 692.0 0 146 293 175 692.0 0 175 294 230 692.0 0 230 295 155 692.0 0 155 296 75 692.0 0 75 297 114 692.0 0 114 298 125 692.0 0 125 299 110 692.0 0 110 300 137 692.0 0 137 301 192 692.0 0 192 Finished in 0.94 s (38 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 24,438 Reads with adapters: 2,139 (8.8%) Reads that were too short: 123 (0.5%) Reads written (passing filters): 2,016 (8.2%) Total basepairs processed: 4,841,700 bp Total written (filtered): 524,651 bp (10.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2139 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 33 0.0 3 0 27 5 1 17 1719 0.0 3 1610 87 16 6 18 54 0.0 3 0 8 3 43 19 4 0.0 3 0 0 4 20 10 0.0 3 2 0 0 8 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 37 8 0.0 3 0 0 0 8 40 2 0.0 3 0 0 1 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 52 3 0.0 3 0 0 0 3 54 1 0.0 3 0 0 0 1 55 9 0.0 3 0 0 0 9 60 2 0.0 3 0 0 0 2 61 5 0.0 3 0 0 0 5 62 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 68 3 0.0 3 0 0 0 3 69 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 26 0.0 3 0 0 1 25 77 50 0.0 3 0 0 0 50 78 2 0.0 3 0 0 0 2 79 5 0.0 3 0 0 0 5 80 10 0.0 3 0 0 1 9 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 4 0.0 3 0 0 0 4 84 2 0.0 3 0 0 1 1 86 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 93 32 0.0 3 0 0 1 31 94 7 0.0 3 0 0 0 7 95 1 0.0 3 0 0 0 1 96 6 0.0 3 0 0 0 6 97 2 0.0 3 0 0 0 2 98 2 0.0 3 0 0 0 2 99 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 144 3 0.0 3 0 0 0 3 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 2 0.0 3 0 0 0 2 175 2 0.0 3 0 0 0 2 176 1 0.0 3 0 0 0 1 182 3 0.0 3 0 0 0 3 185 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 188 20 0.0 3 0 0 0 20 189 30 0.0 3 0 0 0 30 190 2 0.0 3 0 0 0 2 191 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 194 2 0.0 3 0 0 0 2 195 2 0.0 3 0 0 0 2 197 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 2,016 Reads with adapters: 9 (0.4%) Reads written (passing filters): 2,016 (100.0%) Total basepairs processed: 524,651 bp Total written (filtered): 524,623 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 9 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 55.6% C: 22.2% G: 0.0% T: 22.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 8 31.5 0 8 4 1 7.9 0 1