This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.21 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 27,611 (1.0%) Reads that were too short: 11,709 (0.4%) Reads written (passing filters): 15,902 (0.6%) Total basepairs processed: 853,219,115 bp Total written (filtered): 3,312,183 bp (0.4%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 27611 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 44 692.0 0 44 7 97 692.0 0 97 8 1635 692.0 0 1635 9 1838 692.0 0 1838 10 25 692.0 0 25 11 25 692.0 0 25 12 39 692.0 0 39 13 44 692.0 0 44 14 14 692.0 0 14 15 26 692.0 0 26 16 24 692.0 0 24 17 20 692.0 0 20 18 19 692.0 0 19 19 34 692.0 0 34 20 18 692.0 0 18 21 25 692.0 0 25 22 22 692.0 0 22 23 33 692.0 0 33 24 39 692.0 0 39 25 19 692.0 0 19 26 28 692.0 0 28 27 18 692.0 0 18 28 31 692.0 0 31 29 17 692.0 0 17 30 31 692.0 0 31 31 25 692.0 0 25 32 26 692.0 0 26 33 27 692.0 0 27 34 30 692.0 0 30 35 32 692.0 0 32 36 42 692.0 0 42 37 26 692.0 0 26 38 109 692.0 0 109 39 21 692.0 0 21 40 42 692.0 0 42 41 31 692.0 0 31 42 42 692.0 0 42 43 24 692.0 0 24 44 22 692.0 0 22 45 22 692.0 0 22 46 27 692.0 0 27 47 25 692.0 0 25 48 26 692.0 0 26 49 23 692.0 0 23 50 28 692.0 0 28 51 37 692.0 0 37 52 35 692.0 0 35 53 51 692.0 0 51 54 23 692.0 0 23 55 28 692.0 0 28 56 28 692.0 0 28 57 31 692.0 0 31 58 23 692.0 0 23 59 29 692.0 0 29 60 33 692.0 0 33 61 40 692.0 0 40 62 25 692.0 0 25 63 37 692.0 0 37 64 30 692.0 0 30 65 26 692.0 0 26 66 41 692.0 0 41 67 46 692.0 0 46 68 37 692.0 0 37 69 28 692.0 0 28 70 24 692.0 0 24 71 28 692.0 0 28 72 38 692.0 0 38 73 26 692.0 0 26 74 48 692.0 0 48 75 47 692.0 0 47 76 58 692.0 0 58 77 19 692.0 0 19 78 31 692.0 0 31 79 39 692.0 0 39 80 40 692.0 0 40 81 26 692.0 0 26 82 29 692.0 0 29 83 30 692.0 0 30 84 35 692.0 0 35 85 34 692.0 0 34 86 17 692.0 0 17 87 40 692.0 0 40 88 30 692.0 0 30 89 39 692.0 0 39 90 37 692.0 0 37 91 93 692.0 0 93 92 44 692.0 0 44 93 43 692.0 0 43 94 48 692.0 0 48 95 167 692.0 0 167 96 62 692.0 0 62 97 335 692.0 0 335 98 321 692.0 0 321 99 261 692.0 0 261 100 334 692.0 0 334 101 46 692.0 0 46 102 28 692.0 0 28 103 38 692.0 0 38 104 41 692.0 0 41 105 46 692.0 0 46 106 43 692.0 0 43 107 40 692.0 0 40 108 67 692.0 0 67 109 76 692.0 0 76 110 64 692.0 0 64 111 573 692.0 0 573 112 1743 692.0 0 1743 113 467 692.0 0 467 114 28 692.0 0 28 115 22 692.0 0 22 116 28 692.0 0 28 117 41 692.0 0 41 118 37 692.0 0 37 119 33 692.0 0 33 120 29 692.0 0 29 121 29 692.0 0 29 122 30 692.0 0 30 123 39 692.0 0 39 124 34 692.0 0 34 125 34 692.0 0 34 126 33 692.0 0 33 127 29 692.0 0 29 128 48 692.0 0 48 129 39 692.0 0 39 130 81 692.0 0 81 131 38 692.0 0 38 132 42 692.0 0 42 133 33 692.0 0 33 134 73 692.0 0 73 135 43 692.0 0 43 136 49 692.0 0 49 137 37 692.0 0 37 138 31 692.0 0 31 139 34 692.0 0 34 140 36 692.0 0 36 141 51 692.0 0 51 142 35 692.0 0 35 143 26 692.0 0 26 144 39 692.0 0 39 145 31 692.0 0 31 146 78 692.0 0 78 147 57 692.0 0 57 148 38 692.0 0 38 149 49 692.0 0 49 150 42 692.0 0 42 151 27 692.0 0 27 152 30 692.0 0 30 153 37 692.0 0 37 154 77 692.0 0 77 155 110 692.0 0 110 156 243 692.0 0 243 157 100 692.0 0 100 158 28 692.0 0 28 159 23 692.0 0 23 160 29 692.0 0 29 161 36 692.0 0 36 162 38 692.0 0 38 163 27 692.0 0 27 164 47 692.0 0 47 165 35 692.0 0 35 166 169 692.0 0 169 167 121 692.0 0 121 168 105 692.0 0 105 169 24 692.0 0 24 170 35 692.0 0 35 171 26 692.0 0 26 172 26 692.0 0 26 173 28 692.0 0 28 174 25 692.0 0 25 175 26 692.0 0 26 176 39 692.0 0 39 177 36 692.0 0 36 178 35 692.0 0 35 179 46 692.0 0 46 180 28 692.0 0 28 181 84 692.0 0 84 182 38 692.0 0 38 183 40 692.0 0 40 184 35 692.0 0 35 185 33 692.0 0 33 186 45 692.0 0 45 187 33 692.0 0 33 188 40 692.0 0 40 189 39 692.0 0 39 190 37 692.0 0 37 191 40 692.0 0 40 192 49 692.0 0 49 193 100 692.0 0 100 194 211 692.0 0 211 195 291 692.0 0 291 196 133 692.0 0 133 197 63 692.0 0 63 198 61 692.0 0 61 199 68 692.0 0 68 200 117 692.0 0 117 201 75 692.0 0 75 202 74 692.0 0 74 203 37 692.0 0 37 204 39 692.0 0 39 205 38 692.0 0 38 206 35 692.0 0 35 207 58 692.0 0 58 208 51 692.0 0 51 209 34 692.0 0 34 210 48 692.0 0 48 211 32 692.0 0 32 212 30 692.0 0 30 213 43 692.0 0 43 214 47 692.0 0 47 215 47 692.0 0 47 216 75 692.0 0 75 217 60 692.0 0 60 218 49 692.0 0 49 219 40 692.0 0 40 220 253 692.0 0 253 221 192 692.0 0 192 222 530 692.0 0 530 223 139 692.0 0 139 224 35 692.0 0 35 225 43 692.0 0 43 226 42 692.0 0 42 227 47 692.0 0 47 228 46 692.0 0 46 229 40 692.0 0 40 230 38 692.0 0 38 231 31 692.0 0 31 232 37 692.0 0 37 233 44 692.0 0 44 234 41 692.0 0 41 235 48 692.0 0 48 236 53 692.0 0 53 237 136 692.0 0 136 238 146 692.0 0 146 239 249 692.0 0 249 240 125 692.0 0 125 241 81 692.0 0 81 242 114 692.0 0 114 243 207 692.0 0 207 244 106 692.0 0 106 245 147 692.0 0 147 246 65 692.0 0 65 247 49 692.0 0 49 248 58 692.0 0 58 249 81 692.0 0 81 250 60 692.0 0 60 251 69 692.0 0 69 252 134 692.0 0 134 253 114 692.0 0 114 254 206 692.0 0 206 255 135 692.0 0 135 256 282 692.0 0 282 257 64 692.0 0 64 258 48 692.0 0 48 259 166 692.0 0 166 260 63 692.0 0 63 261 79 692.0 0 79 262 733 692.0 0 733 263 872 692.0 0 872 264 1482 692.0 0 1482 265 59 692.0 0 59 266 40 692.0 0 40 267 33 692.0 0 33 268 39 692.0 0 39 269 104 692.0 0 104 270 75 692.0 0 75 271 52 692.0 0 52 272 59 692.0 0 59 273 58 692.0 0 58 274 81 692.0 0 81 275 80 692.0 0 80 276 89 692.0 0 89 277 37 692.0 0 37 278 65 692.0 0 65 279 89 692.0 0 89 280 106 692.0 0 106 281 114 692.0 0 114 282 79 692.0 0 79 283 51 692.0 0 51 284 47 692.0 0 47 285 47 692.0 0 47 286 60 692.0 0 60 287 36 692.0 0 36 288 84 692.0 0 84 289 142 692.0 0 142 290 116 692.0 0 116 291 130 692.0 0 130 292 93 692.0 0 93 293 162 692.0 0 162 294 224 692.0 0 224 295 186 692.0 0 186 296 70 692.0 0 70 297 89 692.0 0 89 298 71 692.0 0 71 299 95 692.0 0 95 300 86 692.0 0 86 301 94 692.0 0 94 Finished in 0.70 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 15,902 Reads with adapters: 1,932 (12.1%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 1,926 (12.1%) Total basepairs processed: 3,312,183 bp Total written (filtered): 522,637 bp (15.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1932 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 3 0.0 3 0 0 3 19 35 0.0 3 0 35 20 1853 0.0 4 1812 38 3 21 7 0.0 4 3 4 22 1 0.0 4 0 0 1 23 12 0.0 4 0 0 0 12 24 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 1 45 1 0.0 4 0 0 0 0 1 71 2 0.0 4 0 0 0 0 2 110 1 0.0 4 0 0 0 0 1 127 11 0.0 4 0 0 0 0 11 130 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 1,926 Reads with adapters: 189 (9.8%) Reads written (passing filters): 1,926 (100.0%) Total basepairs processed: 522,637 bp Total written (filtered): 519,637 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 189 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.7% C: 2.1% G: 93.1% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 7.5 0 1 9 22 0.0 0 0 22 10 151 0.0 1 5 146 11 10 0.0 1 0 10 150 1 0.0 1 0 1 257 4 0.0 1 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.