This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.54 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 177,144 (6.2%) Reads that were too short: 18,529 (0.7%) Reads written (passing filters): 158,615 (5.6%) Total basepairs processed: 853,219,115 bp Total written (filtered): 35,142,463 bp (4.1%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 177144 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 425 692.0 0 425 7 3816 692.0 0 3816 8 3063 692.0 0 3063 9 55 692.0 0 55 10 63 692.0 0 63 11 344 692.0 0 344 12 56 692.0 0 56 13 92 692.0 0 92 14 84 692.0 0 84 15 44 692.0 0 44 16 70 692.0 0 70 17 54 692.0 0 54 18 79 692.0 0 79 19 99 692.0 0 99 20 85 692.0 0 85 21 68 692.0 0 68 22 106 692.0 0 106 23 91 692.0 0 91 24 76 692.0 0 76 25 83 692.0 0 83 26 129 692.0 0 129 27 64 692.0 0 64 28 118 692.0 0 118 29 309 692.0 0 309 30 294 692.0 0 294 31 59 692.0 0 59 32 59 692.0 0 59 33 82 692.0 0 82 34 63 692.0 0 63 35 63 692.0 0 63 36 97 692.0 0 97 37 112 692.0 0 112 38 160 692.0 0 160 39 369 692.0 0 369 40 145 692.0 0 145 41 240 692.0 0 240 42 130 692.0 0 130 43 125 692.0 0 125 44 231 692.0 0 231 45 125 692.0 0 125 46 142 692.0 0 142 47 138 692.0 0 138 48 152 692.0 0 152 49 235 692.0 0 235 50 235 692.0 0 235 51 430 692.0 0 430 52 594 692.0 0 594 53 862 692.0 0 862 54 1803 692.0 0 1803 55 3234 692.0 0 3234 56 45009 692.0 0 45009 57 27938 692.0 0 27938 58 26451 692.0 0 26451 59 180 692.0 0 180 60 84 692.0 0 84 61 111 692.0 0 111 62 352 692.0 0 352 63 161 692.0 0 161 64 252 692.0 0 252 65 52 692.0 0 52 66 81 692.0 0 81 67 80 692.0 0 80 68 67 692.0 0 67 69 65 692.0 0 65 70 60 692.0 0 60 71 90 692.0 0 90 72 91 692.0 0 91 73 75 692.0 0 75 74 99 692.0 0 99 75 65 692.0 0 65 76 69 692.0 0 69 77 47 692.0 0 47 78 63 692.0 0 63 79 51 692.0 0 51 80 92 692.0 0 92 81 78 692.0 0 78 82 91 692.0 0 91 83 203 692.0 0 203 84 95 692.0 0 95 85 104 692.0 0 104 86 123 692.0 0 123 87 166 692.0 0 166 88 259 692.0 0 259 89 149 692.0 0 149 90 95 692.0 0 95 91 73 692.0 0 73 92 61 692.0 0 61 93 74 692.0 0 74 94 75 692.0 0 75 95 52 692.0 0 52 96 62 692.0 0 62 97 70 692.0 0 70 98 47 692.0 0 47 99 119 692.0 0 119 100 91 692.0 0 91 101 62 692.0 0 62 102 52 692.0 0 52 103 206 692.0 0 206 104 112 692.0 0 112 105 197 692.0 0 197 106 62 692.0 0 62 107 281 692.0 0 281 108 69 692.0 0 69 109 68 692.0 0 68 110 60 692.0 0 60 111 89 692.0 0 89 112 86 692.0 0 86 113 169 692.0 0 169 114 75 692.0 0 75 115 59 692.0 0 59 116 50 692.0 0 50 117 56 692.0 0 56 118 59 692.0 0 59 119 73 692.0 0 73 120 71 692.0 0 71 121 85 692.0 0 85 122 41 692.0 0 41 123 96 692.0 0 96 124 196 692.0 0 196 125 195 692.0 0 195 126 403 692.0 0 403 127 105 692.0 0 105 128 213 692.0 0 213 129 148 692.0 0 148 130 73 692.0 0 73 131 167 692.0 0 167 132 89 692.0 0 89 133 87 692.0 0 87 134 86 692.0 0 86 135 85 692.0 0 85 136 132 692.0 0 132 137 56 692.0 0 56 138 69 692.0 0 69 139 264 692.0 0 264 140 111 692.0 0 111 141 103 692.0 0 103 142 120 692.0 0 120 143 130 692.0 0 130 144 93 692.0 0 93 145 174 692.0 0 174 146 225 692.0 0 225 147 1823 692.0 0 1823 148 1657 692.0 0 1657 149 1161 692.0 0 1161 150 629 692.0 0 629 151 296 692.0 0 296 152 333 692.0 0 333 153 102 692.0 0 102 154 120 692.0 0 120 155 61 692.0 0 61 156 98 692.0 0 98 157 75 692.0 0 75 158 84 692.0 0 84 159 93 692.0 0 93 160 389 692.0 0 389 161 154 692.0 0 154 162 161 692.0 0 161 163 80 692.0 0 80 164 83 692.0 0 83 165 124 692.0 0 124 166 183 692.0 0 183 167 1551 692.0 0 1551 168 767 692.0 0 767 169 1062 692.0 0 1062 170 112 692.0 0 112 171 182 692.0 0 182 172 284 692.0 0 284 173 640 692.0 0 640 174 206 692.0 0 206 175 793 692.0 0 793 176 327 692.0 0 327 177 490 692.0 0 490 178 232 692.0 0 232 179 81 692.0 0 81 180 106 692.0 0 106 181 93 692.0 0 93 182 115 692.0 0 115 183 164 692.0 0 164 184 113 692.0 0 113 185 123 692.0 0 123 186 250 692.0 0 250 187 215 692.0 0 215 188 347 692.0 0 347 189 142 692.0 0 142 190 103 692.0 0 103 191 154 692.0 0 154 192 196 692.0 0 196 193 300 692.0 0 300 194 461 692.0 0 461 195 1020 692.0 0 1020 196 741 692.0 0 741 197 1604 692.0 0 1604 198 380 692.0 0 380 199 640 692.0 0 640 200 5513 692.0 0 5513 201 2476 692.0 0 2476 202 2967 692.0 0 2967 203 106 692.0 0 106 204 56 692.0 0 56 205 67 692.0 0 67 206 62 692.0 0 62 207 86 692.0 0 86 208 80 692.0 0 80 209 50 692.0 0 50 210 46 692.0 0 46 211 44 692.0 0 44 212 43 692.0 0 43 213 63 692.0 0 63 214 65 692.0 0 65 215 106 692.0 0 106 216 74 692.0 0 74 217 58 692.0 0 58 218 107 692.0 0 107 219 285 692.0 0 285 220 162 692.0 0 162 221 260 692.0 0 260 222 73 692.0 0 73 223 64 692.0 0 64 224 65 692.0 0 65 225 67 692.0 0 67 226 59 692.0 0 59 227 88 692.0 0 88 228 104 692.0 0 104 229 213 692.0 0 213 230 284 692.0 0 284 231 377 692.0 0 377 232 909 692.0 0 909 233 324 692.0 0 324 234 481 692.0 0 481 235 59 692.0 0 59 236 52 692.0 0 52 237 80 692.0 0 80 238 74 692.0 0 74 239 93 692.0 0 93 240 99 692.0 0 99 241 113 692.0 0 113 242 80 692.0 0 80 243 71 692.0 0 71 244 63 692.0 0 63 245 46 692.0 0 46 246 92 692.0 0 92 247 68 692.0 0 68 248 127 692.0 0 127 249 105 692.0 0 105 250 89 692.0 0 89 251 67 692.0 0 67 252 69 692.0 0 69 253 52 692.0 0 52 254 63 692.0 0 63 255 68 692.0 0 68 256 65 692.0 0 65 257 106 692.0 0 106 258 74 692.0 0 74 259 82 692.0 0 82 260 93 692.0 0 93 261 108 692.0 0 108 262 102 692.0 0 102 263 85 692.0 0 85 264 101 692.0 0 101 265 83 692.0 0 83 266 104 692.0 0 104 267 133 692.0 0 133 268 377 692.0 0 377 269 313 692.0 0 313 270 412 692.0 0 412 271 157 692.0 0 157 272 94 692.0 0 94 273 157 692.0 0 157 274 128 692.0 0 128 275 132 692.0 0 132 276 206 692.0 0 206 277 156 692.0 0 156 278 249 692.0 0 249 279 159 692.0 0 159 280 485 692.0 0 485 281 473 692.0 0 473 282 311 692.0 0 311 283 748 692.0 0 748 284 115 692.0 0 115 285 57 692.0 0 57 286 45 692.0 0 45 287 177 692.0 0 177 288 185 692.0 0 185 289 493 692.0 0 493 290 149 692.0 0 149 291 113 692.0 0 113 292 213 692.0 0 213 293 315 692.0 0 315 294 270 692.0 0 270 295 210 692.0 0 210 296 104 692.0 0 104 297 100 692.0 0 100 298 63 692.0 0 63 299 83 692.0 0 83 300 328 692.0 0 328 301 291 692.0 0 291 Finished in 6.02 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 158,615 Reads with adapters: 4,294 (2.7%) Reads that were too short: 214 (0.1%) Reads written (passing filters): 4,080 (2.6%) Total basepairs processed: 35,142,463 bp Total written (filtered): 1,097,336 bp (3.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4294 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 2 0.0 3 0 0 1 1 16 38 0.0 3 0 32 4 2 17 3775 0.0 3 3539 198 33 5 18 7 0.0 3 0 5 0 2 19 3 0.0 3 0 0 2 1 20 59 0.0 3 2 2 2 53 22 5 0.0 3 0 0 0 5 23 3 0.0 3 0 0 0 3 25 2 0.0 3 0 0 0 2 26 2 0.0 3 0 0 0 2 27 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 30 17 0.0 3 0 0 0 17 32 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 1 1 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 16 0.0 3 0 1 6 9 46 5 0.0 3 0 0 0 5 47 11 0.0 3 0 0 0 11 48 4 0.0 3 0 0 0 4 49 1 0.0 3 0 0 0 1 50 3 0.0 3 0 0 0 3 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 7 0.0 3 0 0 0 7 55 1 0.0 3 0 0 0 1 56 3 0.0 3 0 0 0 3 57 2 0.0 3 0 0 0 2 58 4 0.0 3 0 0 0 4 59 11 0.0 3 0 0 0 11 61 3 0.0 3 0 0 0 3 65 4 0.0 3 0 0 0 4 66 2 0.0 3 0 0 1 1 67 3 0.0 3 0 0 0 3 68 7 0.0 3 0 0 0 7 71 3 0.0 3 0 0 0 3 73 2 0.0 3 0 0 0 2 77 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 0 2 81 4 0.0 3 0 0 0 4 87 1 0.0 3 0 0 0 1 88 4 0.0 3 0 0 0 4 89 2 0.0 3 0 0 1 1 91 1 0.0 3 0 0 0 1 92 6 0.0 3 0 0 0 6 93 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 99 2 0.0 3 0 0 0 2 100 2 0.0 3 0 1 0 1 102 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 3 0.0 3 0 0 0 3 115 1 0.0 3 0 0 0 1 116 12 0.0 3 0 0 0 12 117 3 0.0 3 0 0 0 3 118 3 0.0 3 0 0 1 2 119 3 0.0 3 0 0 0 3 120 4 0.0 3 0 0 0 4 121 3 0.0 3 0 0 0 3 124 3 0.0 3 0 0 0 3 127 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 1 136 22 0.0 3 0 0 0 22 138 1 0.0 3 0 0 0 1 139 3 0.0 3 0 0 0 3 140 3 0.0 3 0 0 0 3 141 3 0.0 3 0 0 0 3 142 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 145 41 0.0 3 0 0 1 40 146 3 0.0 3 0 0 0 3 152 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 160 2 0.0 3 0 0 0 2 162 2 0.0 3 0 0 0 2 163 3 0.0 3 0 0 0 3 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 168 2 0.0 3 0 0 0 2 170 1 0.0 3 0 0 0 1 171 2 0.0 3 0 0 0 2 172 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 177 4 0.0 3 0 0 0 4 178 3 0.0 3 0 0 0 3 180 5 0.0 3 0 0 0 5 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 3 0.0 3 0 0 0 3 188 2 0.0 3 0 0 0 2 197 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 203 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 221 16 0.0 3 0 0 0 16 222 2 0.0 3 0 0 0 2 223 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 229 4 0.0 3 0 0 0 4 230 7 0.0 3 0 0 0 7 231 13 0.0 3 0 0 0 13 232 10 0.0 3 0 0 0 10 233 2 0.0 3 0 0 1 1 237 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 239 2 0.0 3 0 0 0 2 240 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 4,080 Reads with adapters: 42 (1.0%) Reads written (passing filters): 4,080 (100.0%) Total basepairs processed: 1,097,336 bp Total written (filtered): 1,097,208 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 42 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 9.5% C: 59.5% G: 28.6% T: 2.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 41 63.8 0 41 5 1 4.0 0 1