This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.39 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 179,532 (6.3%) Reads that were too short: 14,309 (0.5%) Reads written (passing filters): 165,223 (5.8%) Total basepairs processed: 853,219,115 bp Total written (filtered): 37,384,495 bp (4.4%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 179532 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 307 692.0 0 307 7 3127 692.0 0 3127 8 4117 692.0 0 4117 9 61 692.0 0 61 10 46 692.0 0 46 11 87 692.0 0 87 12 68 692.0 0 68 13 66 692.0 0 66 14 60 692.0 0 60 15 75 692.0 0 75 16 75 692.0 0 75 17 67 692.0 0 67 18 79 692.0 0 79 19 128 692.0 0 128 20 92 692.0 0 92 21 85 692.0 0 85 22 100 692.0 0 100 23 86 692.0 0 86 24 85 692.0 0 85 25 77 692.0 0 77 26 120 692.0 0 120 27 70 692.0 0 70 28 137 692.0 0 137 29 327 692.0 0 327 30 318 692.0 0 318 31 59 692.0 0 59 32 59 692.0 0 59 33 67 692.0 0 67 34 68 692.0 0 68 35 70 692.0 0 70 36 108 692.0 0 108 37 115 692.0 0 115 38 192 692.0 0 192 39 422 692.0 0 422 40 151 692.0 0 151 41 210 692.0 0 210 42 116 692.0 0 116 43 137 692.0 0 137 44 283 692.0 0 283 45 154 692.0 0 154 46 169 692.0 0 169 47 170 692.0 0 170 48 166 692.0 0 166 49 238 692.0 0 238 50 236 692.0 0 236 51 397 692.0 0 397 52 619 692.0 0 619 53 844 692.0 0 844 54 1901 692.0 0 1901 55 2677 692.0 0 2677 56 52062 692.0 0 52062 57 30737 692.0 0 30737 58 30564 692.0 0 30564 59 187 692.0 0 187 60 61 692.0 0 61 61 60 692.0 0 60 62 140 692.0 0 140 63 81 692.0 0 81 64 119 692.0 0 119 65 60 692.0 0 60 66 63 692.0 0 63 67 61 692.0 0 61 68 63 692.0 0 63 69 97 692.0 0 97 70 63 692.0 0 63 71 90 692.0 0 90 72 70 692.0 0 70 73 85 692.0 0 85 74 88 692.0 0 88 75 58 692.0 0 58 76 69 692.0 0 69 77 57 692.0 0 57 78 56 692.0 0 56 79 73 692.0 0 73 80 58 692.0 0 58 81 63 692.0 0 63 82 72 692.0 0 72 83 97 692.0 0 97 84 82 692.0 0 82 85 71 692.0 0 71 86 80 692.0 0 80 87 120 692.0 0 120 88 145 692.0 0 145 89 112 692.0 0 112 90 57 692.0 0 57 91 68 692.0 0 68 92 63 692.0 0 63 93 63 692.0 0 63 94 66 692.0 0 66 95 43 692.0 0 43 96 59 692.0 0 59 97 70 692.0 0 70 98 53 692.0 0 53 99 114 692.0 0 114 100 90 692.0 0 90 101 56 692.0 0 56 102 53 692.0 0 53 103 166 692.0 0 166 104 98 692.0 0 98 105 146 692.0 0 146 106 60 692.0 0 60 107 344 692.0 0 344 108 68 692.0 0 68 109 51 692.0 0 51 110 67 692.0 0 67 111 94 692.0 0 94 112 95 692.0 0 95 113 82 692.0 0 82 114 62 692.0 0 62 115 48 692.0 0 48 116 73 692.0 0 73 117 51 692.0 0 51 118 54 692.0 0 54 119 70 692.0 0 70 120 72 692.0 0 72 121 98 692.0 0 98 122 45 692.0 0 45 123 65 692.0 0 65 124 114 692.0 0 114 125 144 692.0 0 144 126 263 692.0 0 263 127 86 692.0 0 86 128 183 692.0 0 183 129 133 692.0 0 133 130 74 692.0 0 74 131 207 692.0 0 207 132 79 692.0 0 79 133 61 692.0 0 61 134 80 692.0 0 80 135 89 692.0 0 89 136 131 692.0 0 131 137 43 692.0 0 43 138 84 692.0 0 84 139 285 692.0 0 285 140 88 692.0 0 88 141 79 692.0 0 79 142 103 692.0 0 103 143 94 692.0 0 94 144 83 692.0 0 83 145 109 692.0 0 109 146 128 692.0 0 128 147 711 692.0 0 711 148 976 692.0 0 976 149 626 692.0 0 626 150 468 692.0 0 468 151 281 692.0 0 281 152 253 692.0 0 253 153 74 692.0 0 74 154 109 692.0 0 109 155 50 692.0 0 50 156 90 692.0 0 90 157 62 692.0 0 62 158 58 692.0 0 58 159 77 692.0 0 77 160 205 692.0 0 205 161 106 692.0 0 106 162 89 692.0 0 89 163 98 692.0 0 98 164 80 692.0 0 80 165 135 692.0 0 135 166 110 692.0 0 110 167 1364 692.0 0 1364 168 615 692.0 0 615 169 909 692.0 0 909 170 94 692.0 0 94 171 107 692.0 0 107 172 131 692.0 0 131 173 364 692.0 0 364 174 127 692.0 0 127 175 351 692.0 0 351 176 157 692.0 0 157 177 184 692.0 0 184 178 89 692.0 0 89 179 56 692.0 0 56 180 89 692.0 0 89 181 93 692.0 0 93 182 76 692.0 0 76 183 132 692.0 0 132 184 118 692.0 0 118 185 104 692.0 0 104 186 188 692.0 0 188 187 224 692.0 0 224 188 311 692.0 0 311 189 126 692.0 0 126 190 126 692.0 0 126 191 129 692.0 0 129 192 139 692.0 0 139 193 220 692.0 0 220 194 167 692.0 0 167 195 451 692.0 0 451 196 367 692.0 0 367 197 591 692.0 0 591 198 369 692.0 0 369 199 716 692.0 0 716 200 7168 692.0 0 7168 201 3157 692.0 0 3157 202 4432 692.0 0 4432 203 111 692.0 0 111 204 63 692.0 0 63 205 55 692.0 0 55 206 61 692.0 0 61 207 56 692.0 0 56 208 65 692.0 0 65 209 53 692.0 0 53 210 53 692.0 0 53 211 64 692.0 0 64 212 63 692.0 0 63 213 55 692.0 0 55 214 58 692.0 0 58 215 116 692.0 0 116 216 70 692.0 0 70 217 59 692.0 0 59 218 68 692.0 0 68 219 129 692.0 0 129 220 93 692.0 0 93 221 99 692.0 0 99 222 53 692.0 0 53 223 48 692.0 0 48 224 54 692.0 0 54 225 56 692.0 0 56 226 55 692.0 0 55 227 56 692.0 0 56 228 81 692.0 0 81 229 157 692.0 0 157 230 185 692.0 0 185 231 236 692.0 0 236 232 465 692.0 0 465 233 200 692.0 0 200 234 227 692.0 0 227 235 64 692.0 0 64 236 60 692.0 0 60 237 59 692.0 0 59 238 93 692.0 0 93 239 74 692.0 0 74 240 86 692.0 0 86 241 89 692.0 0 89 242 56 692.0 0 56 243 88 692.0 0 88 244 51 692.0 0 51 245 51 692.0 0 51 246 58 692.0 0 58 247 47 692.0 0 47 248 73 692.0 0 73 249 69 692.0 0 69 250 68 692.0 0 68 251 68 692.0 0 68 252 71 692.0 0 71 253 41 692.0 0 41 254 49 692.0 0 49 255 51 692.0 0 51 256 63 692.0 0 63 257 114 692.0 0 114 258 55 692.0 0 55 259 69 692.0 0 69 260 75 692.0 0 75 261 116 692.0 0 116 262 47 692.0 0 47 263 56 692.0 0 56 264 61 692.0 0 61 265 71 692.0 0 71 266 56 692.0 0 56 267 75 692.0 0 75 268 127 692.0 0 127 269 106 692.0 0 106 270 158 692.0 0 158 271 89 692.0 0 89 272 75 692.0 0 75 273 144 692.0 0 144 274 97 692.0 0 97 275 128 692.0 0 128 276 124 692.0 0 124 277 110 692.0 0 110 278 211 692.0 0 211 279 91 692.0 0 91 280 480 692.0 0 480 281 219 692.0 0 219 282 177 692.0 0 177 283 377 692.0 0 377 284 47 692.0 0 47 285 54 692.0 0 54 286 49 692.0 0 49 287 105 692.0 0 105 288 121 692.0 0 121 289 165 692.0 0 165 290 152 692.0 0 152 291 73 692.0 0 73 292 72 692.0 0 72 293 124 692.0 0 124 294 91 692.0 0 91 295 90 692.0 0 90 296 80 692.0 0 80 297 83 692.0 0 83 298 65 692.0 0 65 299 52 692.0 0 52 300 139 692.0 0 139 301 114 692.0 0 114 Finished in 7.13 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 165,223 Reads with adapters: 4,332 (2.6%) Reads that were too short: 21 (0.0%) Reads written (passing filters): 4,311 (2.6%) Total basepairs processed: 37,384,495 bp Total written (filtered): 1,172,504 bp (3.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4332 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 3 0.0 3 0 0 3 19 48 0.0 3 0 48 20 4063 0.0 4 3997 66 21 13 0.0 4 2 11 22 3 0.0 4 0 1 2 23 138 0.0 4 133 1 0 3 1 24 1 0.0 4 1 26 1 0.0 4 0 0 0 1 56 1 0.0 4 0 0 1 69 1 0.0 4 0 0 0 0 1 74 1 0.0 4 1 78 1 0.0 4 1 79 1 0.0 4 1 93 1 0.0 4 1 96 1 0.0 4 0 0 0 0 1 100 1 0.0 4 1 103 1 0.0 4 1 104 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 1 122 2 0.0 4 1 0 0 0 1 123 1 0.0 4 0 0 0 0 1 127 32 0.0 4 0 0 0 0 32 144 1 0.0 4 1 145 1 0.0 4 1 169 1 0.0 4 0 0 0 0 1 175 2 0.0 4 0 0 0 0 2 202 6 0.0 4 0 0 0 1 5 226 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 1 237 2 0.0 4 0 1 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.14 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 4,311 Reads with adapters: 393 (9.1%) Reads written (passing filters): 4,311 (100.0%) Total basepairs processed: 1,172,504 bp Total written (filtered): 1,167,438 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 393 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.3% C: 4.8% G: 91.3% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 67.4 0 3 4 1 16.8 0 1 9 6 0.0 0 0 6 10 52 0.0 1 3 49 11 323 0.0 1 1 322 90 6 0.0 1 5 1 177 1 0.0 1 1 209 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.