This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.62 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 149,231 (5.3%) Reads that were too short: 11,259 (0.4%) Reads written (passing filters): 137,972 (4.9%) Total basepairs processed: 853,219,115 bp Total written (filtered): 33,390,475 bp (3.9%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 149231 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 209 692.0 0 209 7 9198 692.0 0 9198 8 180 692.0 0 180 9 8075 692.0 0 8075 10 64 692.0 0 64 11 50 692.0 0 50 12 68 692.0 0 68 13 77 692.0 0 77 14 57 692.0 0 57 15 65 692.0 0 65 16 108 692.0 0 108 17 93 692.0 0 93 18 105 692.0 0 105 19 76 692.0 0 76 20 95 692.0 0 95 21 74 692.0 0 74 22 84 692.0 0 84 23 84 692.0 0 84 24 79 692.0 0 79 25 89 692.0 0 89 26 83 692.0 0 83 27 133 692.0 0 133 28 198 692.0 0 198 29 316 692.0 0 316 30 129 692.0 0 129 31 148 692.0 0 148 32 382 692.0 0 382 33 145 692.0 0 145 34 543 692.0 0 543 35 169 692.0 0 169 36 121 692.0 0 121 37 139 692.0 0 139 38 289 692.0 0 289 39 186 692.0 0 186 40 169 692.0 0 169 41 192 692.0 0 192 42 2986 692.0 0 2986 43 4960 692.0 0 4960 44 5020 692.0 0 5020 45 121 692.0 0 121 46 82 692.0 0 82 47 193 692.0 0 193 48 337 692.0 0 337 49 202 692.0 0 202 50 216 692.0 0 216 51 156 692.0 0 156 52 155 692.0 0 155 53 195 692.0 0 195 54 98 692.0 0 98 55 139 692.0 0 139 56 93 692.0 0 93 57 122 692.0 0 122 58 166 692.0 0 166 59 188 692.0 0 188 60 286 692.0 0 286 61 507 692.0 0 507 62 593 692.0 0 593 63 1378 692.0 0 1378 64 2653 692.0 0 2653 65 56611 692.0 0 56611 66 10514 692.0 0 10514 67 18137 692.0 0 18137 68 138 692.0 0 138 69 96 692.0 0 96 70 64 692.0 0 64 71 74 692.0 0 74 72 63 692.0 0 63 73 77 692.0 0 77 74 62 692.0 0 62 75 80 692.0 0 80 76 60 692.0 0 60 77 73 692.0 0 73 78 72 692.0 0 72 79 61 692.0 0 61 80 78 692.0 0 78 81 73 692.0 0 73 82 63 692.0 0 63 83 79 692.0 0 79 84 63 692.0 0 63 85 70 692.0 0 70 86 75 692.0 0 75 87 67 692.0 0 67 88 77 692.0 0 77 89 141 692.0 0 141 90 180 692.0 0 180 91 78 692.0 0 78 92 71 692.0 0 71 93 82 692.0 0 82 94 91 692.0 0 91 95 87 692.0 0 87 96 75 692.0 0 75 97 69 692.0 0 69 98 79 692.0 0 79 99 71 692.0 0 71 100 84 692.0 0 84 101 97 692.0 0 97 102 87 692.0 0 87 103 87 692.0 0 87 104 69 692.0 0 69 105 84 692.0 0 84 106 90 692.0 0 90 107 83 692.0 0 83 108 101 692.0 0 101 109 60 692.0 0 60 110 76 692.0 0 76 111 84 692.0 0 84 112 70 692.0 0 70 113 74 692.0 0 74 114 71 692.0 0 71 115 61 692.0 0 61 116 52 692.0 0 52 117 65 692.0 0 65 118 79 692.0 0 79 119 69 692.0 0 69 120 58 692.0 0 58 121 68 692.0 0 68 122 59 692.0 0 59 123 61 692.0 0 61 124 82 692.0 0 82 125 70 692.0 0 70 126 75 692.0 0 75 127 65 692.0 0 65 128 69 692.0 0 69 129 64 692.0 0 64 130 58 692.0 0 58 131 81 692.0 0 81 132 80 692.0 0 80 133 84 692.0 0 84 134 114 692.0 0 114 135 64 692.0 0 64 136 156 692.0 0 156 137 93 692.0 0 93 138 277 692.0 0 277 139 75 692.0 0 75 140 71 692.0 0 71 141 67 692.0 0 67 142 53 692.0 0 53 143 60 692.0 0 60 144 54 692.0 0 54 145 57 692.0 0 57 146 57 692.0 0 57 147 140 692.0 0 140 148 53 692.0 0 53 149 59 692.0 0 59 150 51 692.0 0 51 151 47 692.0 0 47 152 51 692.0 0 51 153 55 692.0 0 55 154 51 692.0 0 51 155 48 692.0 0 48 156 62 692.0 0 62 157 58 692.0 0 58 158 54 692.0 0 54 159 60 692.0 0 60 160 61 692.0 0 61 161 55 692.0 0 55 162 67 692.0 0 67 163 71 692.0 0 71 164 56 692.0 0 56 165 51 692.0 0 51 166 60 692.0 0 60 167 46 692.0 0 46 168 59 692.0 0 59 169 63 692.0 0 63 170 46 692.0 0 46 171 40 692.0 0 40 172 61 692.0 0 61 173 57 692.0 0 57 174 71 692.0 0 71 175 59 692.0 0 59 176 42 692.0 0 42 177 43 692.0 0 43 178 63 692.0 0 63 179 66 692.0 0 66 180 72 692.0 0 72 181 55 692.0 0 55 182 52 692.0 0 52 183 63 692.0 0 63 184 76 692.0 0 76 185 88 692.0 0 88 186 64 692.0 0 64 187 46 692.0 0 46 188 74 692.0 0 74 189 70 692.0 0 70 190 57 692.0 0 57 191 67 692.0 0 67 192 40 692.0 0 40 193 62 692.0 0 62 194 53 692.0 0 53 195 59 692.0 0 59 196 44 692.0 0 44 197 64 692.0 0 64 198 73 692.0 0 73 199 47 692.0 0 47 200 67 692.0 0 67 201 69 692.0 0 69 202 65 692.0 0 65 203 90 692.0 0 90 204 63 692.0 0 63 205 58 692.0 0 58 206 60 692.0 0 60 207 49 692.0 0 49 208 63 692.0 0 63 209 68 692.0 0 68 210 71 692.0 0 71 211 52 692.0 0 52 212 61 692.0 0 61 213 71 692.0 0 71 214 59 692.0 0 59 215 87 692.0 0 87 216 83 692.0 0 83 217 135 692.0 0 135 218 60 692.0 0 60 219 83 692.0 0 83 220 53 692.0 0 53 221 52 692.0 0 52 222 134 692.0 0 134 223 121 692.0 0 121 224 169 692.0 0 169 225 117 692.0 0 117 226 50 692.0 0 50 227 59 692.0 0 59 228 80 692.0 0 80 229 52 692.0 0 52 230 59 692.0 0 59 231 50 692.0 0 50 232 49 692.0 0 49 233 53 692.0 0 53 234 43 692.0 0 43 235 57 692.0 0 57 236 62 692.0 0 62 237 69 692.0 0 69 238 80 692.0 0 80 239 42 692.0 0 42 240 75 692.0 0 75 241 75 692.0 0 75 242 138 692.0 0 138 243 132 692.0 0 132 244 158 692.0 0 158 245 90 692.0 0 90 246 88 692.0 0 88 247 82 692.0 0 82 248 70 692.0 0 70 249 83 692.0 0 83 250 91 692.0 0 91 251 101 692.0 0 101 252 114 692.0 0 114 253 115 692.0 0 115 254 145 692.0 0 145 255 719 692.0 0 719 256 506 692.0 0 506 257 593 692.0 0 593 258 156 692.0 0 156 259 103 692.0 0 103 260 375 692.0 0 375 261 1025 692.0 0 1025 262 457 692.0 0 457 263 119 692.0 0 119 264 62 692.0 0 62 265 74 692.0 0 74 266 122 692.0 0 122 267 71 692.0 0 71 268 38 692.0 0 38 269 70 692.0 0 70 270 207 692.0 0 207 271 158 692.0 0 158 272 152 692.0 0 152 273 51 692.0 0 51 274 80 692.0 0 80 275 48 692.0 0 48 276 48 692.0 0 48 277 68 692.0 0 68 278 97 692.0 0 97 279 71 692.0 0 71 280 50 692.0 0 50 281 60 692.0 0 60 282 63 692.0 0 63 283 53 692.0 0 53 284 58 692.0 0 58 285 60 692.0 0 60 286 62 692.0 0 62 287 60 692.0 0 60 288 67 692.0 0 67 289 42 692.0 0 42 290 84 692.0 0 84 291 77 692.0 0 77 292 101 692.0 0 101 293 62 692.0 0 62 294 63 692.0 0 63 295 40 692.0 0 40 296 55 692.0 0 55 297 73 692.0 0 73 298 64 692.0 0 64 299 100 692.0 0 100 300 106 692.0 0 106 301 103 692.0 0 103 Finished in 6.35 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 137,972 Reads with adapters: 9,342 (6.8%) Reads that were too short: 46 (0.0%) Reads written (passing filters): 9,296 (6.7%) Total basepairs processed: 33,390,475 bp Total written (filtered): 2,535,264 bp (7.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 9342 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 4 1 19 116 0.0 3 0 110 6 20 9047 0.0 4 8876 167 4 21 10 0.0 4 1 9 22 4 0.0 4 0 0 4 23 5 0.0 4 0 0 0 4 1 24 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 1 55 1 0.0 4 1 80 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 113 3 0.0 4 0 0 0 0 3 114 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 121 2 0.0 4 2 123 1 0.0 4 0 0 1 126 2 0.0 4 0 0 0 0 2 127 90 0.0 4 0 0 0 0 90 137 2 0.0 4 2 149 1 0.0 4 0 0 0 0 1 168 2 0.0 4 0 0 0 0 2 173 1 0.0 4 0 0 0 0 1 190 1 0.0 4 1 193 17 0.0 4 0 0 0 0 17 194 7 0.0 4 0 0 0 0 7 195 1 0.0 4 0 0 0 0 1 198 1 0.0 4 1 214 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 0 6 229 3 0.0 4 0 0 0 0 3 230 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 244 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.31 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 9,296 Reads with adapters: 278 (3.0%) Reads written (passing filters): 9,296 (100.0%) Total basepairs processed: 2,535,264 bp Total written (filtered): 2,496,847 bp (98.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 278 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 11.5% C: 4.0% G: 25.2% T: 59.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 28 145.2 0 28 4 2 36.3 0 2 10 5 0.0 1 0 5 11 51 0.0 1 2 49 12 24 0.0 1 0 24 15 1 0.0 1 0 1 54 1 0.0 1 0 1 225 159 0.0 1 147 12 226 7 0.0 1 6 1