This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTATGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.57 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 66,128 (2.3%) Reads that were too short: 24,461 (0.9%) Reads written (passing filters): 41,667 (1.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 8,552,870 bp (1.0%) === Adapter 1 === Sequence: GTATGT; Type: regular 5'; Length: 6; Trimmed: 66128 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1636 692.0 0 1636 7 185 692.0 0 185 8 5707 692.0 0 5707 9 5210 692.0 0 5210 10 45 692.0 0 45 11 31 692.0 0 31 12 44 692.0 0 44 13 36 692.0 0 36 14 38 692.0 0 38 15 38 692.0 0 38 16 44 692.0 0 44 17 57 692.0 0 57 18 54 692.0 0 54 19 52 692.0 0 52 20 40 692.0 0 40 21 38 692.0 0 38 22 64 692.0 0 64 23 66 692.0 0 66 24 82 692.0 0 82 25 93 692.0 0 93 26 70 692.0 0 70 27 56 692.0 0 56 28 45 692.0 0 45 29 92 692.0 0 92 30 49 692.0 0 49 31 69 692.0 0 69 32 39 692.0 0 39 33 37 692.0 0 37 34 57 692.0 0 57 35 36 692.0 0 36 36 101 692.0 0 101 37 36 692.0 0 36 38 50 692.0 0 50 39 35 692.0 0 35 40 32 692.0 0 32 41 36 692.0 0 36 42 45 692.0 0 45 43 47 692.0 0 47 44 30 692.0 0 30 45 38 692.0 0 38 46 46 692.0 0 46 47 44 692.0 0 44 48 39 692.0 0 39 49 45 692.0 0 45 50 47 692.0 0 47 51 40 692.0 0 40 52 49 692.0 0 49 53 43 692.0 0 43 54 40 692.0 0 40 55 39 692.0 0 39 56 45 692.0 0 45 57 58 692.0 0 58 58 59 692.0 0 59 59 36 692.0 0 36 60 47 692.0 0 47 61 33 692.0 0 33 62 50 692.0 0 50 63 47 692.0 0 47 64 42 692.0 0 42 65 76 692.0 0 76 66 40 692.0 0 40 67 81 692.0 0 81 68 49 692.0 0 49 69 413 692.0 0 413 70 58 692.0 0 58 71 189 692.0 0 189 72 53 692.0 0 53 73 49 692.0 0 49 74 38 692.0 0 38 75 77 692.0 0 77 76 101 692.0 0 101 77 116 692.0 0 116 78 224 692.0 0 224 79 98 692.0 0 98 80 297 692.0 0 297 81 120 692.0 0 120 82 298 692.0 0 298 83 102 692.0 0 102 84 67 692.0 0 67 85 76 692.0 0 76 86 93 692.0 0 93 87 88 692.0 0 88 88 53 692.0 0 53 89 71 692.0 0 71 90 98 692.0 0 98 91 52 692.0 0 52 92 63 692.0 0 63 93 54 692.0 0 54 94 106 692.0 0 106 95 127 692.0 0 127 96 1285 692.0 0 1285 97 726 692.0 0 726 98 1586 692.0 0 1586 99 80 692.0 0 80 100 55 692.0 0 55 101 61 692.0 0 61 102 42 692.0 0 42 103 57 692.0 0 57 104 46 692.0 0 46 105 52 692.0 0 52 106 45 692.0 0 45 107 47 692.0 0 47 108 69 692.0 0 69 109 41 692.0 0 41 110 52 692.0 0 52 111 61 692.0 0 61 112 72 692.0 0 72 113 91 692.0 0 91 114 99 692.0 0 99 115 58 692.0 0 58 116 51 692.0 0 51 117 61 692.0 0 61 118 52 692.0 0 52 119 55 692.0 0 55 120 92 692.0 0 92 121 50 692.0 0 50 122 71 692.0 0 71 123 48 692.0 0 48 124 74 692.0 0 74 125 53 692.0 0 53 126 270 692.0 0 270 127 199 692.0 0 199 128 515 692.0 0 515 129 160 692.0 0 160 130 238 692.0 0 238 131 138 692.0 0 138 132 481 692.0 0 481 133 419 692.0 0 419 134 991 692.0 0 991 135 162 692.0 0 162 136 84 692.0 0 84 137 55 692.0 0 55 138 66 692.0 0 66 139 109 692.0 0 109 140 69 692.0 0 69 141 74 692.0 0 74 142 49 692.0 0 49 143 52 692.0 0 52 144 64 692.0 0 64 145 52 692.0 0 52 146 54 692.0 0 54 147 68 692.0 0 68 148 346 692.0 0 346 149 535 692.0 0 535 150 333 692.0 0 333 151 50 692.0 0 50 152 46 692.0 0 46 153 71 692.0 0 71 154 30 692.0 0 30 155 55 692.0 0 55 156 54 692.0 0 54 157 66 692.0 0 66 158 75 692.0 0 75 159 67 692.0 0 67 160 65 692.0 0 65 161 69 692.0 0 69 162 61 692.0 0 61 163 47 692.0 0 47 164 58 692.0 0 58 165 54 692.0 0 54 166 64 692.0 0 64 167 56 692.0 0 56 168 34 692.0 0 34 169 61 692.0 0 61 170 50 692.0 0 50 171 62 692.0 0 62 172 80 692.0 0 80 173 99 692.0 0 99 174 145 692.0 0 145 175 159 692.0 0 159 176 71 692.0 0 71 177 88 692.0 0 88 178 98 692.0 0 98 179 87 692.0 0 87 180 70 692.0 0 70 181 100 692.0 0 100 182 87 692.0 0 87 183 77 692.0 0 77 184 125 692.0 0 125 185 210 692.0 0 210 186 261 692.0 0 261 187 1276 692.0 0 1276 188 2402 692.0 0 2402 189 3765 692.0 0 3765 190 959 692.0 0 959 191 61 692.0 0 61 192 68 692.0 0 68 193 47 692.0 0 47 194 62 692.0 0 62 195 49 692.0 0 49 196 69 692.0 0 69 197 64 692.0 0 64 198 65 692.0 0 65 199 60 692.0 0 60 200 56 692.0 0 56 201 61 692.0 0 61 202 66 692.0 0 66 203 74 692.0 0 74 204 56 692.0 0 56 205 86 692.0 0 86 206 88 692.0 0 88 207 130 692.0 0 130 208 111 692.0 0 111 209 760 692.0 0 760 210 701 692.0 0 701 211 2092 692.0 0 2092 212 148 692.0 0 148 213 67 692.0 0 67 214 72 692.0 0 72 215 63 692.0 0 63 216 45 692.0 0 45 217 58 692.0 0 58 218 57 692.0 0 57 219 58 692.0 0 58 220 69 692.0 0 69 221 55 692.0 0 55 222 66 692.0 0 66 223 80 692.0 0 80 224 83 692.0 0 83 225 118 692.0 0 118 226 263 692.0 0 263 227 109 692.0 0 109 228 169 692.0 0 169 229 62 692.0 0 62 230 165 692.0 0 165 231 100 692.0 0 100 232 116 692.0 0 116 233 195 692.0 0 195 234 202 692.0 0 202 235 249 692.0 0 249 236 205 692.0 0 205 237 110 692.0 0 110 238 95 692.0 0 95 239 109 692.0 0 109 240 480 692.0 0 480 241 352 692.0 0 352 242 762 692.0 0 762 243 264 692.0 0 264 244 177 692.0 0 177 245 124 692.0 0 124 246 181 692.0 0 181 247 296 692.0 0 296 248 851 692.0 0 851 249 449 692.0 0 449 250 210 692.0 0 210 251 388 692.0 0 388 252 202 692.0 0 202 253 461 692.0 0 461 254 867 692.0 0 867 255 1058 692.0 0 1058 256 209 692.0 0 209 257 111 692.0 0 111 258 251 692.0 0 251 259 137 692.0 0 137 260 387 692.0 0 387 261 309 692.0 0 309 262 502 692.0 0 502 263 156 692.0 0 156 264 89 692.0 0 89 265 204 692.0 0 204 266 144 692.0 0 144 267 352 692.0 0 352 268 179 692.0 0 179 269 560 692.0 0 560 270 389 692.0 0 389 271 1041 692.0 0 1041 272 715 692.0 0 715 273 477 692.0 0 477 274 1338 692.0 0 1338 275 182 692.0 0 182 276 66 692.0 0 66 277 66 692.0 0 66 278 67 692.0 0 67 279 96 692.0 0 96 280 82 692.0 0 82 281 103 692.0 0 103 282 72 692.0 0 72 283 221 692.0 0 221 284 104 692.0 0 104 285 90 692.0 0 90 286 52 692.0 0 52 287 51 692.0 0 51 288 63 692.0 0 63 289 70 692.0 0 70 290 49 692.0 0 49 291 60 692.0 0 60 292 69 692.0 0 69 293 71 692.0 0 71 294 94 692.0 0 94 295 92 692.0 0 92 296 97 692.0 0 97 297 55 692.0 0 55 298 161 692.0 0 161 299 93 692.0 0 93 300 90 692.0 0 90 301 121 692.0 0 121 Finished in 1.62 s (39 us/read; 1.54 M reads/minute). === Summary === Total reads processed: 41,667 Reads with adapters: 7,331 (17.6%) Reads that were too short: 54 (0.1%) Reads written (passing filters): 7,277 (17.5%) Total basepairs processed: 8,552,870 bp Total written (filtered): 1,982,412 bp (23.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7331 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 16 88 0.0 3 0 73 11 4 17 5403 0.0 3 4989 339 63 12 18 72 0.0 3 0 12 3 57 19 60 0.0 3 14 19 19 8 20 1478 0.0 3 1358 92 13 15 21 4 0.0 3 1 3 22 2 0.0 3 0 0 0 2 23 9 0.0 3 0 0 0 9 33 1 0.0 3 0 0 0 1 34 15 0.0 3 0 0 0 15 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 46 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 59 27 0.0 3 0 0 1 26 61 4 0.0 3 0 0 0 4 62 10 0.0 3 0 0 0 10 65 18 0.0 3 0 0 0 18 66 2 0.0 3 0 0 0 2 68 2 0.0 3 0 0 0 2 70 46 0.0 3 0 0 0 46 73 10 0.0 3 0 0 0 10 75 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 79 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 85 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 94 4 0.0 3 1 0 0 3 96 5 0.0 3 0 0 0 5 97 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 1 1 107 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 114 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 156 2 0.0 3 0 0 0 2 160 5 0.0 3 0 0 0 5 162 2 0.0 3 0 0 0 2 163 2 0.0 3 0 0 1 1 165 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 171 4 0.0 3 0 0 1 3 172 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 216 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.25 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 7,277 Reads with adapters: 54 (0.7%) Reads written (passing filters): 7,277 (100.0%) Total basepairs processed: 1,982,412 bp Total written (filtered): 1,982,248 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 54 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.9% C: 44.4% G: 29.6% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 52 113.7 0 52 4 2 28.4 0 2