This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTATGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.31 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 51,254 (1.8%) Reads that were too short: 14,451 (0.5%) Reads written (passing filters): 36,803 (1.3%) Total basepairs processed: 853,219,115 bp Total written (filtered): 7,609,654 bp (0.9%) === Adapter 1 === Sequence: GTATGT; Type: regular 5'; Length: 6; Trimmed: 51254 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1323 692.0 0 1323 7 151 692.0 0 151 8 5571 692.0 0 5571 9 5783 692.0 0 5783 10 35 692.0 0 35 11 28 692.0 0 28 12 35 692.0 0 35 13 28 692.0 0 28 14 19 692.0 0 19 15 29 692.0 0 29 16 28 692.0 0 28 17 22 692.0 0 22 18 27 692.0 0 27 19 31 692.0 0 31 20 20 692.0 0 20 21 22 692.0 0 22 22 30 692.0 0 30 23 34 692.0 0 34 24 37 692.0 0 37 25 23 692.0 0 23 26 29 692.0 0 29 27 27 692.0 0 27 28 39 692.0 0 39 29 31 692.0 0 31 30 38 692.0 0 38 31 23 692.0 0 23 32 13 692.0 0 13 33 18 692.0 0 18 34 32 692.0 0 32 35 30 692.0 0 30 36 44 692.0 0 44 37 27 692.0 0 27 38 50 692.0 0 50 39 48 692.0 0 48 40 31 692.0 0 31 41 26 692.0 0 26 42 37 692.0 0 37 43 42 692.0 0 42 44 30 692.0 0 30 45 34 692.0 0 34 46 31 692.0 0 31 47 28 692.0 0 28 48 34 692.0 0 34 49 216 692.0 0 216 50 28 692.0 0 28 51 26 692.0 0 26 52 23 692.0 0 23 53 35 692.0 0 35 54 27 692.0 0 27 55 34 692.0 0 34 56 36 692.0 0 36 57 46 692.0 0 46 58 36 692.0 0 36 59 28 692.0 0 28 60 25 692.0 0 25 61 24 692.0 0 24 62 29 692.0 0 29 63 34 692.0 0 34 64 32 692.0 0 32 65 37 692.0 0 37 66 45 692.0 0 45 67 58 692.0 0 58 68 56 692.0 0 56 69 510 692.0 0 510 70 36 692.0 0 36 71 159 692.0 0 159 72 41 692.0 0 41 73 36 692.0 0 36 74 30 692.0 0 30 75 36 692.0 0 36 76 81 692.0 0 81 77 56 692.0 0 56 78 103 692.0 0 103 79 70 692.0 0 70 80 309 692.0 0 309 81 66 692.0 0 66 82 284 692.0 0 284 83 68 692.0 0 68 84 37 692.0 0 37 85 38 692.0 0 38 86 52 692.0 0 52 87 55 692.0 0 55 88 37 692.0 0 37 89 48 692.0 0 48 90 67 692.0 0 67 91 41 692.0 0 41 92 46 692.0 0 46 93 54 692.0 0 54 94 77 692.0 0 77 95 84 692.0 0 84 96 1011 692.0 0 1011 97 536 692.0 0 536 98 1252 692.0 0 1252 99 58 692.0 0 58 100 55 692.0 0 55 101 46 692.0 0 46 102 49 692.0 0 49 103 28 692.0 0 28 104 28 692.0 0 28 105 31 692.0 0 31 106 37 692.0 0 37 107 40 692.0 0 40 108 68 692.0 0 68 109 28 692.0 0 28 110 45 692.0 0 45 111 41 692.0 0 41 112 42 692.0 0 42 113 63 692.0 0 63 114 65 692.0 0 65 115 42 692.0 0 42 116 52 692.0 0 52 117 39 692.0 0 39 118 38 692.0 0 38 119 46 692.0 0 46 120 50 692.0 0 50 121 37 692.0 0 37 122 43 692.0 0 43 123 50 692.0 0 50 124 42 692.0 0 42 125 38 692.0 0 38 126 192 692.0 0 192 127 140 692.0 0 140 128 301 692.0 0 301 129 110 692.0 0 110 130 139 692.0 0 139 131 108 692.0 0 108 132 287 692.0 0 287 133 274 692.0 0 274 134 444 692.0 0 444 135 108 692.0 0 108 136 36 692.0 0 36 137 46 692.0 0 46 138 65 692.0 0 65 139 105 692.0 0 105 140 51 692.0 0 51 141 45 692.0 0 45 142 30 692.0 0 30 143 47 692.0 0 47 144 41 692.0 0 41 145 37 692.0 0 37 146 53 692.0 0 53 147 55 692.0 0 55 148 336 692.0 0 336 149 519 692.0 0 519 150 360 692.0 0 360 151 53 692.0 0 53 152 27 692.0 0 27 153 44 692.0 0 44 154 37 692.0 0 37 155 35 692.0 0 35 156 47 692.0 0 47 157 52 692.0 0 52 158 34 692.0 0 34 159 35 692.0 0 35 160 26 692.0 0 26 161 40 692.0 0 40 162 47 692.0 0 47 163 39 692.0 0 39 164 58 692.0 0 58 165 56 692.0 0 56 166 43 692.0 0 43 167 37 692.0 0 37 168 44 692.0 0 44 169 39 692.0 0 39 170 41 692.0 0 41 171 39 692.0 0 39 172 66 692.0 0 66 173 79 692.0 0 79 174 130 692.0 0 130 175 147 692.0 0 147 176 56 692.0 0 56 177 83 692.0 0 83 178 71 692.0 0 71 179 56 692.0 0 56 180 62 692.0 0 62 181 71 692.0 0 71 182 66 692.0 0 66 183 65 692.0 0 65 184 106 692.0 0 106 185 147 692.0 0 147 186 179 692.0 0 179 187 1177 692.0 0 1177 188 2325 692.0 0 2325 189 4187 692.0 0 4187 190 1050 692.0 0 1050 191 47 692.0 0 47 192 44 692.0 0 44 193 44 692.0 0 44 194 51 692.0 0 51 195 37 692.0 0 37 196 42 692.0 0 42 197 44 692.0 0 44 198 40 692.0 0 40 199 44 692.0 0 44 200 34 692.0 0 34 201 37 692.0 0 37 202 31 692.0 0 31 203 63 692.0 0 63 204 42 692.0 0 42 205 53 692.0 0 53 206 59 692.0 0 59 207 76 692.0 0 76 208 75 692.0 0 75 209 648 692.0 0 648 210 390 692.0 0 390 211 1343 692.0 0 1343 212 96 692.0 0 96 213 43 692.0 0 43 214 47 692.0 0 47 215 34 692.0 0 34 216 40 692.0 0 40 217 49 692.0 0 49 218 47 692.0 0 47 219 56 692.0 0 56 220 52 692.0 0 52 221 36 692.0 0 36 222 43 692.0 0 43 223 74 692.0 0 74 224 55 692.0 0 55 225 88 692.0 0 88 226 190 692.0 0 190 227 67 692.0 0 67 228 92 692.0 0 92 229 36 692.0 0 36 230 91 692.0 0 91 231 52 692.0 0 52 232 71 692.0 0 71 233 116 692.0 0 116 234 100 692.0 0 100 235 129 692.0 0 129 236 126 692.0 0 126 237 70 692.0 0 70 238 75 692.0 0 75 239 65 692.0 0 65 240 262 692.0 0 262 241 181 692.0 0 181 242 342 692.0 0 342 243 121 692.0 0 121 244 114 692.0 0 114 245 87 692.0 0 87 246 110 692.0 0 110 247 309 692.0 0 309 248 940 692.0 0 940 249 249 692.0 0 249 250 125 692.0 0 125 251 263 692.0 0 263 252 172 692.0 0 172 253 338 692.0 0 338 254 624 692.0 0 624 255 776 692.0 0 776 256 142 692.0 0 142 257 61 692.0 0 61 258 91 692.0 0 91 259 61 692.0 0 61 260 205 692.0 0 205 261 147 692.0 0 147 262 196 692.0 0 196 263 53 692.0 0 53 264 59 692.0 0 59 265 96 692.0 0 96 266 80 692.0 0 80 267 172 692.0 0 172 268 140 692.0 0 140 269 234 692.0 0 234 270 142 692.0 0 142 271 402 692.0 0 402 272 222 692.0 0 222 273 152 692.0 0 152 274 333 692.0 0 333 275 79 692.0 0 79 276 35 692.0 0 35 277 44 692.0 0 44 278 53 692.0 0 53 279 44 692.0 0 44 280 58 692.0 0 58 281 70 692.0 0 70 282 43 692.0 0 43 283 102 692.0 0 102 284 67 692.0 0 67 285 45 692.0 0 45 286 40 692.0 0 40 287 37 692.0 0 37 288 43 692.0 0 43 289 38 692.0 0 38 290 42 692.0 0 42 291 53 692.0 0 53 292 46 692.0 0 46 293 54 692.0 0 54 294 73 692.0 0 73 295 69 692.0 0 69 296 62 692.0 0 62 297 56 692.0 0 56 298 101 692.0 0 101 299 57 692.0 0 57 300 50 692.0 0 50 301 169 692.0 0 169 Finished in 1.57 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 36,803 Reads with adapters: 6,064 (16.5%) Reads that were too short: 36 (0.1%) Reads written (passing filters): 6,028 (16.4%) Total basepairs processed: 7,609,654 bp Total written (filtered): 1,638,870 bp (21.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6064 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 2 0.0 3 0 0 1 1 19 120 0.0 3 0 113 6 0 1 20 5879 0.0 4 5751 124 1 1 2 21 9 0.0 4 2 7 22 1 0.0 4 1 23 2 0.0 4 0 0 0 1 1 24 2 0.0 4 1 0 0 0 1 41 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 55 3 0.0 4 0 0 0 1 2 56 11 0.0 4 0 0 0 0 11 57 1 0.0 4 0 0 0 0 1 58 2 0.0 4 0 0 0 0 2 70 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 103 3 0.0 4 0 0 0 0 3 130 6 0.0 4 0 0 0 0 6 133 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 1 138 1 0.0 4 0 0 1 140 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 1 228 5 0.0 4 0 0 0 0 5 230 1 0.0 4 0 0 0 0 1 243 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.21 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 6,028 Reads with adapters: 765 (12.7%) Reads written (passing filters): 6,028 (100.0%) Total basepairs processed: 1,638,870 bp Total written (filtered): 1,629,709 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 765 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.6% C: 4.8% G: 90.7% T: 2.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 94.2 0 1 5 1 5.9 0 1 9 270 0.0 0 2 268 10 435 0.0 1 12 423 11 48 0.0 1 2 46 12 2 0.0 1 0 2 223 5 0.0 1 4 1 224 1 0.0 1 1 225 1 0.0 1 1 257 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.