This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.51 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 34,222 (1.2%) Reads that were too short: 13,566 (0.5%) Reads written (passing filters): 20,656 (0.7%) Total basepairs processed: 853,219,115 bp Total written (filtered): 4,101,071 bp (0.5%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 34222 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 102 692.0 0 102 7 1104 692.0 0 1104 8 953 692.0 0 953 9 87 692.0 0 87 10 131 692.0 0 131 11 29 692.0 0 29 12 27 692.0 0 27 13 25 692.0 0 25 14 29 692.0 0 29 15 17 692.0 0 17 16 30 692.0 0 30 17 20 692.0 0 20 18 19 692.0 0 19 19 26 692.0 0 26 20 23 692.0 0 23 21 31 692.0 0 31 22 58 692.0 0 58 23 23 692.0 0 23 24 21 692.0 0 21 25 26 692.0 0 26 26 15 692.0 0 15 27 12 692.0 0 12 28 21 692.0 0 21 29 28 692.0 0 28 30 27 692.0 0 27 31 30 692.0 0 30 32 27 692.0 0 27 33 16 692.0 0 16 34 24 692.0 0 24 35 26 692.0 0 26 36 33 692.0 0 33 37 29 692.0 0 29 38 45 692.0 0 45 39 31 692.0 0 31 40 60 692.0 0 60 41 31 692.0 0 31 42 25 692.0 0 25 43 31 692.0 0 31 44 26 692.0 0 26 45 32 692.0 0 32 46 34 692.0 0 34 47 30 692.0 0 30 48 476 692.0 0 476 49 86 692.0 0 86 50 29 692.0 0 29 51 26 692.0 0 26 52 27 692.0 0 27 53 32 692.0 0 32 54 22 692.0 0 22 55 26 692.0 0 26 56 26 692.0 0 26 57 26 692.0 0 26 58 22 692.0 0 22 59 35 692.0 0 35 60 81 692.0 0 81 61 37 692.0 0 37 62 35 692.0 0 35 63 28 692.0 0 28 64 28 692.0 0 28 65 39 692.0 0 39 66 29 692.0 0 29 67 30 692.0 0 30 68 46 692.0 0 46 69 525 692.0 0 525 70 160 692.0 0 160 71 379 692.0 0 379 72 216 692.0 0 216 73 467 692.0 0 467 74 218 692.0 0 218 75 67 692.0 0 67 76 24 692.0 0 24 77 33 692.0 0 33 78 39 692.0 0 39 79 106 692.0 0 106 80 114 692.0 0 114 81 357 692.0 0 357 82 133 692.0 0 133 83 301 692.0 0 301 84 702 692.0 0 702 85 1886 692.0 0 1886 86 1721 692.0 0 1721 87 30 692.0 0 30 88 67 692.0 0 67 89 39 692.0 0 39 90 56 692.0 0 56 91 52 692.0 0 52 92 25 692.0 0 25 93 26 692.0 0 26 94 38 692.0 0 38 95 31 692.0 0 31 96 29 692.0 0 29 97 29 692.0 0 29 98 31 692.0 0 31 99 54 692.0 0 54 100 34 692.0 0 34 101 38 692.0 0 38 102 31 692.0 0 31 103 25 692.0 0 25 104 20 692.0 0 20 105 13 692.0 0 13 106 16 692.0 0 16 107 30 692.0 0 30 108 22 692.0 0 22 109 26 692.0 0 26 110 49 692.0 0 49 111 92 692.0 0 92 112 110 692.0 0 110 113 36 692.0 0 36 114 25 692.0 0 25 115 27 692.0 0 27 116 15 692.0 0 15 117 20 692.0 0 20 118 20 692.0 0 20 119 38 692.0 0 38 120 23 692.0 0 23 121 47 692.0 0 47 122 24 692.0 0 24 123 23 692.0 0 23 124 31 692.0 0 31 125 32 692.0 0 32 126 19 692.0 0 19 127 24 692.0 0 24 128 25 692.0 0 25 129 48 692.0 0 48 130 77 692.0 0 77 131 52 692.0 0 52 132 51 692.0 0 51 133 36 692.0 0 36 134 27 692.0 0 27 135 31 692.0 0 31 136 29 692.0 0 29 137 47 692.0 0 47 138 60 692.0 0 60 139 36 692.0 0 36 140 28 692.0 0 28 141 39 692.0 0 39 142 68 692.0 0 68 143 44 692.0 0 44 144 38 692.0 0 38 145 91 692.0 0 91 146 72 692.0 0 72 147 71 692.0 0 71 148 163 692.0 0 163 149 91 692.0 0 91 150 97 692.0 0 97 151 82 692.0 0 82 152 80 692.0 0 80 153 54 692.0 0 54 154 22 692.0 0 22 155 32 692.0 0 32 156 60 692.0 0 60 157 86 692.0 0 86 158 132 692.0 0 132 159 72 692.0 0 72 160 32 692.0 0 32 161 41 692.0 0 41 162 35 692.0 0 35 163 27 692.0 0 27 164 23 692.0 0 23 165 34 692.0 0 34 166 42 692.0 0 42 167 45 692.0 0 45 168 96 692.0 0 96 169 88 692.0 0 88 170 123 692.0 0 123 171 49 692.0 0 49 172 38 692.0 0 38 173 43 692.0 0 43 174 65 692.0 0 65 175 123 692.0 0 123 176 914 692.0 0 914 177 296 692.0 0 296 178 451 692.0 0 451 179 33 692.0 0 33 180 35 692.0 0 35 181 31 692.0 0 31 182 34 692.0 0 34 183 39 692.0 0 39 184 35 692.0 0 35 185 42 692.0 0 42 186 39 692.0 0 39 187 86 692.0 0 86 188 164 692.0 0 164 189 139 692.0 0 139 190 58 692.0 0 58 191 42 692.0 0 42 192 29 692.0 0 29 193 43 692.0 0 43 194 63 692.0 0 63 195 104 692.0 0 104 196 206 692.0 0 206 197 269 692.0 0 269 198 489 692.0 0 489 199 564 692.0 0 564 200 82 692.0 0 82 201 59 692.0 0 59 202 573 692.0 0 573 203 344 692.0 0 344 204 575 692.0 0 575 205 450 692.0 0 450 206 188 692.0 0 188 207 51 692.0 0 51 208 46 692.0 0 46 209 90 692.0 0 90 210 134 692.0 0 134 211 108 692.0 0 108 212 111 692.0 0 111 213 215 692.0 0 215 214 267 692.0 0 267 215 393 692.0 0 393 216 71 692.0 0 71 217 90 692.0 0 90 218 105 692.0 0 105 219 54 692.0 0 54 220 54 692.0 0 54 221 40 692.0 0 40 222 156 692.0 0 156 223 670 692.0 0 670 224 884 692.0 0 884 225 534 692.0 0 534 226 48 692.0 0 48 227 40 692.0 0 40 228 47 692.0 0 47 229 30 692.0 0 30 230 39 692.0 0 39 231 27 692.0 0 27 232 21 692.0 0 21 233 59 692.0 0 59 234 50 692.0 0 50 235 59 692.0 0 59 236 51 692.0 0 51 237 59 692.0 0 59 238 87 692.0 0 87 239 81 692.0 0 81 240 57 692.0 0 57 241 123 692.0 0 123 242 78 692.0 0 78 243 95 692.0 0 95 244 156 692.0 0 156 245 109 692.0 0 109 246 98 692.0 0 98 247 65 692.0 0 65 248 60 692.0 0 60 249 56 692.0 0 56 250 66 692.0 0 66 251 43 692.0 0 43 252 41 692.0 0 41 253 55 692.0 0 55 254 65 692.0 0 65 255 152 692.0 0 152 256 36 692.0 0 36 257 63 692.0 0 63 258 287 692.0 0 287 259 130 692.0 0 130 260 211 692.0 0 211 261 94 692.0 0 94 262 59 692.0 0 59 263 53 692.0 0 53 264 52 692.0 0 52 265 37 692.0 0 37 266 35 692.0 0 35 267 33 692.0 0 33 268 35 692.0 0 35 269 50 692.0 0 50 270 117 692.0 0 117 271 495 692.0 0 495 272 160 692.0 0 160 273 155 692.0 0 155 274 44 692.0 0 44 275 54 692.0 0 54 276 49 692.0 0 49 277 59 692.0 0 59 278 59 692.0 0 59 279 104 692.0 0 104 280 51 692.0 0 51 281 61 692.0 0 61 282 148 692.0 0 148 283 133 692.0 0 133 284 226 692.0 0 226 285 70 692.0 0 70 286 86 692.0 0 86 287 93 692.0 0 93 288 55 692.0 0 55 289 48 692.0 0 48 290 51 692.0 0 51 291 73 692.0 0 73 292 78 692.0 0 78 293 54 692.0 0 54 294 71 692.0 0 71 295 68 692.0 0 68 296 120 692.0 0 120 297 295 692.0 0 295 298 421 692.0 0 421 299 406 692.0 0 406 300 165 692.0 0 165 301 102 692.0 0 102 Finished in 0.78 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 20,656 Reads with adapters: 1,217 (5.9%) Reads that were too short: 71 (0.3%) Reads written (passing filters): 1,146 (5.5%) Total basepairs processed: 4,101,071 bp Total written (filtered): 304,971 bp (7.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1217 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 0 2 16 13 0.0 3 0 9 1 3 17 1028 0.0 3 942 65 13 8 18 3 0.0 3 1 0 1 1 19 10 0.0 3 4 0 5 1 20 14 0.0 3 5 3 1 5 21 1 0.0 3 0 0 1 23 3 0.0 3 0 0 0 3 24 1 0.0 3 0 0 0 1 25 2 0.0 3 0 0 0 2 29 1 0.0 3 0 0 0 1 34 2 0.0 3 0 0 0 2 35 3 0.0 3 0 0 0 3 39 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 41 20 0.0 3 0 0 1 19 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 6 0.0 3 0 0 0 6 45 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 57 1 0.0 3 1 60 3 0.0 3 0 0 0 3 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 1 74 2 0.0 3 0 1 0 1 78 1 0.0 3 0 0 1 81 3 0.0 3 0 0 0 3 82 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 84 4 0.0 3 0 0 0 4 88 1 0.0 3 0 0 1 90 2 0.0 3 0 0 0 2 92 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 99 4 0.0 3 0 0 0 4 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 111 3 0.0 3 0 0 0 3 112 2 0.0 3 0 0 1 1 113 2 0.0 3 1 0 0 1 114 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 120 6 0.0 3 0 0 0 6 124 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 3 0.0 3 0 0 0 3 139 3 0.0 3 0 0 1 2 140 3 0.0 3 0 0 0 3 150 1 0.0 3 0 0 1 151 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 1 168 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 1 1 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 1 243 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.04 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 1,146 Reads with adapters: 20 (1.7%) Reads written (passing filters): 1,146 (100.0%) Total basepairs processed: 304,971 bp Total written (filtered): 304,911 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 20 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 35.0% C: 60.0% G: 0.0% T: 5.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 20 17.9 0 20