This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.34 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 18,569 (0.7%) Reads that were too short: 5,778 (0.2%) Reads written (passing filters): 12,791 (0.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 2,727,834 bp (0.3%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 18569 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 92 692.0 0 92 7 1014 692.0 0 1014 8 1166 692.0 0 1166 9 45 692.0 0 45 10 23 692.0 0 23 11 34 692.0 0 34 12 35 692.0 0 35 13 19 692.0 0 19 14 41 692.0 0 41 15 21 692.0 0 21 16 19 692.0 0 19 17 21 692.0 0 21 18 22 692.0 0 22 19 30 692.0 0 30 20 29 692.0 0 29 21 21 692.0 0 21 22 31 692.0 0 31 23 25 692.0 0 25 24 16 692.0 0 16 25 24 692.0 0 24 26 15 692.0 0 15 27 20 692.0 0 20 28 26 692.0 0 26 29 22 692.0 0 22 30 28 692.0 0 28 31 29 692.0 0 29 32 15 692.0 0 15 33 16 692.0 0 16 34 19 692.0 0 19 35 20 692.0 0 20 36 26 692.0 0 26 37 36 692.0 0 36 38 28 692.0 0 28 39 34 692.0 0 34 40 27 692.0 0 27 41 31 692.0 0 31 42 18 692.0 0 18 43 24 692.0 0 24 44 23 692.0 0 23 45 34 692.0 0 34 46 27 692.0 0 27 47 27 692.0 0 27 48 29 692.0 0 29 49 34 692.0 0 34 50 47 692.0 0 47 51 24 692.0 0 24 52 41 692.0 0 41 53 24 692.0 0 24 54 32 692.0 0 32 55 46 692.0 0 46 56 31 692.0 0 31 57 28 692.0 0 28 58 30 692.0 0 30 59 24 692.0 0 24 60 33 692.0 0 33 61 41 692.0 0 41 62 29 692.0 0 29 63 17 692.0 0 17 64 24 692.0 0 24 65 33 692.0 0 33 66 31 692.0 0 31 67 35 692.0 0 35 68 46 692.0 0 46 69 370 692.0 0 370 70 113 692.0 0 113 71 435 692.0 0 435 72 196 692.0 0 196 73 486 692.0 0 486 74 237 692.0 0 237 75 68 692.0 0 68 76 27 692.0 0 27 77 41 692.0 0 41 78 29 692.0 0 29 79 104 692.0 0 104 80 89 692.0 0 89 81 384 692.0 0 384 82 129 692.0 0 129 83 328 692.0 0 328 84 795 692.0 0 795 85 201 692.0 0 201 86 167 692.0 0 167 87 25 692.0 0 25 88 23 692.0 0 23 89 26 692.0 0 26 90 21 692.0 0 21 91 26 692.0 0 26 92 25 692.0 0 25 93 26 692.0 0 26 94 39 692.0 0 39 95 34 692.0 0 34 96 24 692.0 0 24 97 25 692.0 0 25 98 23 692.0 0 23 99 46 692.0 0 46 100 30 692.0 0 30 101 19 692.0 0 19 102 32 692.0 0 32 103 24 692.0 0 24 104 25 692.0 0 25 105 28 692.0 0 28 106 22 692.0 0 22 107 29 692.0 0 29 108 22 692.0 0 22 109 18 692.0 0 18 110 29 692.0 0 29 111 97 692.0 0 97 112 88 692.0 0 88 113 21 692.0 0 21 114 20 692.0 0 20 115 14 692.0 0 14 116 28 692.0 0 28 117 25 692.0 0 25 118 24 692.0 0 24 119 41 692.0 0 41 120 30 692.0 0 30 121 36 692.0 0 36 122 24 692.0 0 24 123 18 692.0 0 18 124 33 692.0 0 33 125 31 692.0 0 31 126 20 692.0 0 20 127 21 692.0 0 21 128 21 692.0 0 21 129 33 692.0 0 33 130 53 692.0 0 53 131 25 692.0 0 25 132 27 692.0 0 27 133 36 692.0 0 36 134 36 692.0 0 36 135 33 692.0 0 33 136 25 692.0 0 25 137 28 692.0 0 28 138 19 692.0 0 19 139 31 692.0 0 31 140 20 692.0 0 20 141 22 692.0 0 22 142 36 692.0 0 36 143 23 692.0 0 23 144 51 692.0 0 51 145 25 692.0 0 25 146 24 692.0 0 24 147 61 692.0 0 61 148 163 692.0 0 163 149 66 692.0 0 66 150 37 692.0 0 37 151 28 692.0 0 28 152 27 692.0 0 27 153 31 692.0 0 31 154 26 692.0 0 26 155 27 692.0 0 27 156 25 692.0 0 25 157 33 692.0 0 33 158 81 692.0 0 81 159 44 692.0 0 44 160 26 692.0 0 26 161 29 692.0 0 29 162 19 692.0 0 19 163 28 692.0 0 28 164 19 692.0 0 19 165 27 692.0 0 27 166 30 692.0 0 30 167 29 692.0 0 29 168 71 692.0 0 71 169 70 692.0 0 70 170 59 692.0 0 59 171 30 692.0 0 30 172 20 692.0 0 20 173 19 692.0 0 19 174 23 692.0 0 23 175 44 692.0 0 44 176 228 692.0 0 228 177 106 692.0 0 106 178 139 692.0 0 139 179 31 692.0 0 31 180 28 692.0 0 28 181 18 692.0 0 18 182 45 692.0 0 45 183 24 692.0 0 24 184 29 692.0 0 29 185 31 692.0 0 31 186 24 692.0 0 24 187 48 692.0 0 48 188 60 692.0 0 60 189 53 692.0 0 53 190 37 692.0 0 37 191 33 692.0 0 33 192 37 692.0 0 37 193 30 692.0 0 30 194 25 692.0 0 25 195 30 692.0 0 30 196 65 692.0 0 65 197 91 692.0 0 91 198 158 692.0 0 158 199 222 692.0 0 222 200 50 692.0 0 50 201 26 692.0 0 26 202 138 692.0 0 138 203 99 692.0 0 99 204 187 692.0 0 187 205 120 692.0 0 120 206 44 692.0 0 44 207 45 692.0 0 45 208 24 692.0 0 24 209 31 692.0 0 31 210 59 692.0 0 59 211 55 692.0 0 55 212 32 692.0 0 32 213 57 692.0 0 57 214 88 692.0 0 88 215 98 692.0 0 98 216 27 692.0 0 27 217 47 692.0 0 47 218 45 692.0 0 45 219 27 692.0 0 27 220 27 692.0 0 27 221 35 692.0 0 35 222 44 692.0 0 44 223 166 692.0 0 166 224 310 692.0 0 310 225 226 692.0 0 226 226 32 692.0 0 32 227 26 692.0 0 26 228 42 692.0 0 42 229 28 692.0 0 28 230 28 692.0 0 28 231 18 692.0 0 18 232 21 692.0 0 21 233 33 692.0 0 33 234 14 692.0 0 14 235 25 692.0 0 25 236 27 692.0 0 27 237 37 692.0 0 37 238 43 692.0 0 43 239 33 692.0 0 33 240 51 692.0 0 51 241 40 692.0 0 40 242 41 692.0 0 41 243 43 692.0 0 43 244 62 692.0 0 62 245 38 692.0 0 38 246 37 692.0 0 37 247 45 692.0 0 45 248 59 692.0 0 59 249 40 692.0 0 40 250 44 692.0 0 44 251 30 692.0 0 30 252 33 692.0 0 33 253 32 692.0 0 32 254 38 692.0 0 38 255 61 692.0 0 61 256 28 692.0 0 28 257 35 692.0 0 35 258 153 692.0 0 153 259 90 692.0 0 90 260 165 692.0 0 165 261 65 692.0 0 65 262 29 692.0 0 29 263 25 692.0 0 25 264 25 692.0 0 25 265 22 692.0 0 22 266 22 692.0 0 22 267 20 692.0 0 20 268 19 692.0 0 19 269 20 692.0 0 20 270 87 692.0 0 87 271 219 692.0 0 219 272 91 692.0 0 91 273 52 692.0 0 52 274 28 692.0 0 28 275 24 692.0 0 24 276 20 692.0 0 20 277 23 692.0 0 23 278 36 692.0 0 36 279 44 692.0 0 44 280 28 692.0 0 28 281 38 692.0 0 38 282 45 692.0 0 45 283 38 692.0 0 38 284 139 692.0 0 139 285 43 692.0 0 43 286 45 692.0 0 45 287 54 692.0 0 54 288 36 692.0 0 36 289 26 692.0 0 26 290 23 692.0 0 23 291 30 692.0 0 30 292 25 692.0 0 25 293 37 692.0 0 37 294 46 692.0 0 46 295 46 692.0 0 46 296 37 692.0 0 37 297 128 692.0 0 128 298 145 692.0 0 145 299 172 692.0 0 172 300 64 692.0 0 64 301 59 692.0 0 59 Finished in 0.59 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 12,791 Reads with adapters: 1,225 (9.6%) Reads that were too short: 0 (0.0%) Reads written (passing filters): 1,225 (9.6%) Total basepairs processed: 2,727,834 bp Total written (filtered): 333,595 bp (12.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1225 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 2 19 33 0.0 3 0 33 20 1172 0.0 4 1142 28 2 21 7 0.0 4 2 5 23 2 0.0 4 1 0 0 1 50 1 0.0 4 1 73 1 0.0 4 1 75 1 0.0 4 1 127 5 0.0 4 0 0 0 0 5 151 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.05 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 1,225 Reads with adapters: 116 (9.5%) Reads written (passing filters): 1,225 (100.0%) Total basepairs processed: 333,595 bp Total written (filtered): 332,218 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 116 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.4% C: 1.7% G: 93.1% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 4.8 0 1 9 1 0.0 0 0 1 10 19 0.0 1 1 18 11 93 0.0 1 1 92 12 1 0.0 1 0 1 139 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.