This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TACTTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 72.67 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 166,716 (5.9%) Reads that were too short: 72,633 (2.6%) Reads written (passing filters): 94,083 (3.3%) Total basepairs processed: 853,219,115 bp Total written (filtered): 18,304,076 bp (2.1%) === Adapter 1 === Sequence: TACTTC; Type: regular 5'; Length: 6; Trimmed: 166716 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1769 692.0 0 1769 7 11191 692.0 0 11191 8 198 692.0 0 198 9 12013 692.0 0 12013 10 41 692.0 0 41 11 33 692.0 0 33 12 26 692.0 0 26 13 17 692.0 0 17 14 50 692.0 0 50 15 28 692.0 0 28 16 30 692.0 0 30 17 44 692.0 0 44 18 22 692.0 0 22 19 71 692.0 0 71 20 22 692.0 0 22 21 19 692.0 0 19 22 29 692.0 0 29 23 25 692.0 0 25 24 25 692.0 0 25 25 35 692.0 0 35 26 29 692.0 0 29 27 35 692.0 0 35 28 40 692.0 0 40 29 30 692.0 0 30 30 32 692.0 0 32 31 48 692.0 0 48 32 43 692.0 0 43 33 19 692.0 0 19 34 44 692.0 0 44 35 27 692.0 0 27 36 33 692.0 0 33 37 46 692.0 0 46 38 60 692.0 0 60 39 46 692.0 0 46 40 32 692.0 0 32 41 27 692.0 0 27 42 32 692.0 0 32 43 40 692.0 0 40 44 32 692.0 0 32 45 25 692.0 0 25 46 27 692.0 0 27 47 45 692.0 0 45 48 43 692.0 0 43 49 28 692.0 0 28 50 38 692.0 0 38 51 22 692.0 0 22 52 27 692.0 0 27 53 24 692.0 0 24 54 34 692.0 0 34 55 18 692.0 0 18 56 57 692.0 0 57 57 52 692.0 0 52 58 97 692.0 0 97 59 43 692.0 0 43 60 35 692.0 0 35 61 31 692.0 0 31 62 48 692.0 0 48 63 32 692.0 0 32 64 31 692.0 0 31 65 31 692.0 0 31 66 22 692.0 0 22 67 39 692.0 0 39 68 26 692.0 0 26 69 22 692.0 0 22 70 26 692.0 0 26 71 125 692.0 0 125 72 82 692.0 0 82 73 151 692.0 0 151 74 23 692.0 0 23 75 41 692.0 0 41 76 56 692.0 0 56 77 33 692.0 0 33 78 44 692.0 0 44 79 48 692.0 0 48 80 27 692.0 0 27 81 32 692.0 0 32 82 60 692.0 0 60 83 49 692.0 0 49 84 31 692.0 0 31 85 71 692.0 0 71 86 53 692.0 0 53 87 172 692.0 0 172 88 66 692.0 0 66 89 166 692.0 0 166 90 155 692.0 0 155 91 101 692.0 0 101 92 40 692.0 0 40 93 46 692.0 0 46 94 37 692.0 0 37 95 43 692.0 0 43 96 45 692.0 0 45 97 51 692.0 0 51 98 46 692.0 0 46 99 30 692.0 0 30 100 40 692.0 0 40 101 53 692.0 0 53 102 57 692.0 0 57 103 339 692.0 0 339 104 241 692.0 0 241 105 196 692.0 0 196 106 123 692.0 0 123 107 72 692.0 0 72 108 73 692.0 0 73 109 69 692.0 0 69 110 35 692.0 0 35 111 72 692.0 0 72 112 69 692.0 0 69 113 85 692.0 0 85 114 53 692.0 0 53 115 48 692.0 0 48 116 73 692.0 0 73 117 42 692.0 0 42 118 57 692.0 0 57 119 86 692.0 0 86 120 68 692.0 0 68 121 107 692.0 0 107 122 46 692.0 0 46 123 98 692.0 0 98 124 46 692.0 0 46 125 65 692.0 0 65 126 87 692.0 0 87 127 122 692.0 0 122 128 423 692.0 0 423 129 284 692.0 0 284 130 80 692.0 0 80 131 170 692.0 0 170 132 192 692.0 0 192 133 141 692.0 0 141 134 346 692.0 0 346 135 387 692.0 0 387 136 164 692.0 0 164 137 130 692.0 0 130 138 119 692.0 0 119 139 165 692.0 0 165 140 517 692.0 0 517 141 361 692.0 0 361 142 433 692.0 0 433 143 489 692.0 0 489 144 724 692.0 0 724 145 1524 692.0 0 1524 146 29966 692.0 0 29966 147 2577 692.0 0 2577 148 8007 692.0 0 8007 149 268 692.0 0 268 150 4297 692.0 0 4297 151 1508 692.0 0 1508 152 2747 692.0 0 2747 153 68 692.0 0 68 154 87 692.0 0 87 155 89 692.0 0 89 156 422 692.0 0 422 157 182 692.0 0 182 158 308 692.0 0 308 159 121 692.0 0 121 160 358 692.0 0 358 161 444 692.0 0 444 162 460 692.0 0 460 163 70 692.0 0 70 164 84 692.0 0 84 165 53 692.0 0 53 166 106 692.0 0 106 167 72 692.0 0 72 168 86 692.0 0 86 169 63 692.0 0 63 170 49 692.0 0 49 171 52 692.0 0 52 172 37 692.0 0 37 173 42 692.0 0 42 174 51 692.0 0 51 175 103 692.0 0 103 176 154 692.0 0 154 177 160 692.0 0 160 178 56 692.0 0 56 179 56 692.0 0 56 180 96 692.0 0 96 181 149 692.0 0 149 182 166 692.0 0 166 183 195 692.0 0 195 184 169 692.0 0 169 185 144 692.0 0 144 186 374 692.0 0 374 187 121 692.0 0 121 188 108 692.0 0 108 189 61 692.0 0 61 190 217 692.0 0 217 191 47 692.0 0 47 192 48 692.0 0 48 193 125 692.0 0 125 194 46 692.0 0 46 195 51 692.0 0 51 196 51 692.0 0 51 197 45 692.0 0 45 198 37 692.0 0 37 199 66 692.0 0 66 200 82 692.0 0 82 201 81 692.0 0 81 202 121 692.0 0 121 203 129 692.0 0 129 204 123 692.0 0 123 205 179 692.0 0 179 206 168 692.0 0 168 207 146 692.0 0 146 208 519 692.0 0 519 209 132 692.0 0 132 210 98 692.0 0 98 211 109 692.0 0 109 212 381 692.0 0 381 213 196 692.0 0 196 214 130 692.0 0 130 215 87 692.0 0 87 216 63 692.0 0 63 217 49 692.0 0 49 218 57 692.0 0 57 219 148 692.0 0 148 220 346 692.0 0 346 221 231 692.0 0 231 222 210 692.0 0 210 223 100 692.0 0 100 224 126 692.0 0 126 225 137 692.0 0 137 226 228 692.0 0 228 227 73 692.0 0 73 228 83 692.0 0 83 229 111 692.0 0 111 230 102 692.0 0 102 231 97 692.0 0 97 232 128 692.0 0 128 233 142 692.0 0 142 234 107 692.0 0 107 235 241 692.0 0 241 236 163 692.0 0 163 237 147 692.0 0 147 238 112 692.0 0 112 239 155 692.0 0 155 240 103 692.0 0 103 241 148 692.0 0 148 242 131 692.0 0 131 243 216 692.0 0 216 244 201 692.0 0 201 245 127 692.0 0 127 246 94 692.0 0 94 247 105 692.0 0 105 248 128 692.0 0 128 249 154 692.0 0 154 250 160 692.0 0 160 251 204 692.0 0 204 252 340 692.0 0 340 253 458 692.0 0 458 254 1089 692.0 0 1089 255 3199 692.0 0 3199 256 24888 692.0 0 24888 257 10820 692.0 0 10820 258 14626 692.0 0 14626 259 1486 692.0 0 1486 260 155 692.0 0 155 261 179 692.0 0 179 262 462 692.0 0 462 263 362 692.0 0 362 264 226 692.0 0 226 265 97 692.0 0 97 266 221 692.0 0 221 267 234 692.0 0 234 268 593 692.0 0 593 269 177 692.0 0 177 270 89 692.0 0 89 271 80 692.0 0 80 272 80 692.0 0 80 273 105 692.0 0 105 274 124 692.0 0 124 275 161 692.0 0 161 276 91 692.0 0 91 277 103 692.0 0 103 278 92 692.0 0 92 279 200 692.0 0 200 280 764 692.0 0 764 281 491 692.0 0 491 282 537 692.0 0 537 283 247 692.0 0 247 284 254 692.0 0 254 285 205 692.0 0 205 286 101 692.0 0 101 287 121 692.0 0 121 288 137 692.0 0 137 289 118 692.0 0 118 290 88 692.0 0 88 291 61 692.0 0 61 292 95 692.0 0 95 293 103 692.0 0 103 294 141 692.0 0 141 295 128 692.0 0 128 296 130 692.0 0 130 297 96 692.0 0 96 298 104 692.0 0 104 299 78 692.0 0 78 300 78 692.0 0 78 301 174 692.0 0 174 Finished in 3.58 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 94,083 Reads with adapters: 12,414 (13.2%) Reads that were too short: 77 (0.1%) Reads written (passing filters): 12,337 (13.1%) Total basepairs processed: 18,304,076 bp Total written (filtered): 3,382,650 bp (18.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12414 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 13 0.0 3 0 0 7 6 16 212 0.0 3 0 191 16 5 17 11916 0.0 3 11107 658 127 24 18 36 0.0 3 0 14 6 16 19 34 0.0 3 0 0 15 19 20 42 0.0 3 3 0 2 37 22 1 0.0 3 0 0 0 1 24 5 0.0 3 0 0 1 4 29 3 0.0 3 0 0 0 3 32 1 0.0 3 0 0 0 1 33 16 0.0 3 0 0 0 16 34 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 36 5 0.0 3 0 0 1 4 37 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 53 7 0.0 3 0 0 5 2 54 3 0.0 3 0 0 0 3 55 1 0.0 3 0 0 1 57 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 1 65 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 1 68 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 75 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 77 5 0.0 3 0 0 0 5 82 2 0.0 3 0 0 0 2 83 8 0.0 3 0 0 5 3 84 2 0.0 3 0 0 0 2 85 2 0.0 3 0 0 0 2 97 2 0.0 3 0 0 0 2 100 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 112 2 0.0 3 0 1 0 1 128 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 143 3 0.0 3 0 0 0 3 144 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 27 0.0 3 0 0 1 26 219 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.44 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 12,337 Reads with adapters: 710 (5.8%) Reads written (passing filters): 12,337 (100.0%) Total basepairs processed: 3,382,650 bp Total written (filtered): 3,380,506 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 710 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 53.7% C: 11.0% G: 31.7% T: 3.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 698 192.8 0 698 4 10 48.2 0 10 5 2 12.0 0 2