This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TACTTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.62 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 128,796 (4.5%) Reads that were too short: 32,407 (1.1%) Reads written (passing filters): 96,389 (3.4%) Total basepairs processed: 853,219,115 bp Total written (filtered): 18,725,717 bp (2.2%) === Adapter 1 === Sequence: TACTTC; Type: regular 5'; Length: 6; Trimmed: 128796 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1871 692.0 0 1871 7 12500 692.0 0 12500 8 144 692.0 0 144 9 11586 692.0 0 11586 10 23 692.0 0 23 11 20 692.0 0 20 12 31 692.0 0 31 13 15 692.0 0 15 14 11 692.0 0 11 15 16 692.0 0 16 16 19 692.0 0 19 17 31 692.0 0 31 18 30 692.0 0 30 19 28 692.0 0 28 20 18 692.0 0 18 21 16 692.0 0 16 22 21 692.0 0 21 23 25 692.0 0 25 24 33 692.0 0 33 25 27 692.0 0 27 26 30 692.0 0 30 27 22 692.0 0 22 28 18 692.0 0 18 29 21 692.0 0 21 30 21 692.0 0 21 31 24 692.0 0 24 32 36 692.0 0 36 33 15 692.0 0 15 34 26 692.0 0 26 35 19 692.0 0 19 36 16 692.0 0 16 37 19 692.0 0 19 38 28 692.0 0 28 39 28 692.0 0 28 40 22 692.0 0 22 41 33 692.0 0 33 42 15 692.0 0 15 43 45 692.0 0 45 44 33 692.0 0 33 45 19 692.0 0 19 46 16 692.0 0 16 47 32 692.0 0 32 48 23 692.0 0 23 49 25 692.0 0 25 50 21 692.0 0 21 51 22 692.0 0 22 52 20 692.0 0 20 53 15 692.0 0 15 54 20 692.0 0 20 55 17 692.0 0 17 56 44 692.0 0 44 57 31 692.0 0 31 58 60 692.0 0 60 59 27 692.0 0 27 60 23 692.0 0 23 61 29 692.0 0 29 62 43 692.0 0 43 63 28 692.0 0 28 64 43 692.0 0 43 65 18 692.0 0 18 66 21 692.0 0 21 67 31 692.0 0 31 68 17 692.0 0 17 69 21 692.0 0 21 70 30 692.0 0 30 71 46 692.0 0 46 72 42 692.0 0 42 73 84 692.0 0 84 74 32 692.0 0 32 75 37 692.0 0 37 76 38 692.0 0 38 77 29 692.0 0 29 78 26 692.0 0 26 79 37 692.0 0 37 80 23 692.0 0 23 81 30 692.0 0 30 82 35 692.0 0 35 83 45 692.0 0 45 84 31 692.0 0 31 85 50 692.0 0 50 86 42 692.0 0 42 87 168 692.0 0 168 88 72 692.0 0 72 89 214 692.0 0 214 90 144 692.0 0 144 91 80 692.0 0 80 92 37 692.0 0 37 93 44 692.0 0 44 94 41 692.0 0 41 95 42 692.0 0 42 96 53 692.0 0 53 97 41 692.0 0 41 98 44 692.0 0 44 99 32 692.0 0 32 100 36 692.0 0 36 101 33 692.0 0 33 102 58 692.0 0 58 103 265 692.0 0 265 104 188 692.0 0 188 105 151 692.0 0 151 106 107 692.0 0 107 107 70 692.0 0 70 108 46 692.0 0 46 109 73 692.0 0 73 110 38 692.0 0 38 111 37 692.0 0 37 112 55 692.0 0 55 113 65 692.0 0 65 114 34 692.0 0 34 115 38 692.0 0 38 116 63 692.0 0 63 117 38 692.0 0 38 118 46 692.0 0 46 119 101 692.0 0 101 120 57 692.0 0 57 121 73 692.0 0 73 122 49 692.0 0 49 123 88 692.0 0 88 124 45 692.0 0 45 125 42 692.0 0 42 126 70 692.0 0 70 127 64 692.0 0 64 128 208 692.0 0 208 129 225 692.0 0 225 130 86 692.0 0 86 131 138 692.0 0 138 132 229 692.0 0 229 133 117 692.0 0 117 134 342 692.0 0 342 135 373 692.0 0 373 136 144 692.0 0 144 137 93 692.0 0 93 138 118 692.0 0 118 139 96 692.0 0 96 140 367 692.0 0 367 141 262 692.0 0 262 142 326 692.0 0 326 143 456 692.0 0 456 144 690 692.0 0 690 145 1558 692.0 0 1558 146 32985 692.0 0 32985 147 2829 692.0 0 2829 148 9058 692.0 0 9058 149 274 692.0 0 274 150 4834 692.0 0 4834 151 1647 692.0 0 1647 152 3112 692.0 0 3112 153 62 692.0 0 62 154 45 692.0 0 45 155 59 692.0 0 59 156 407 692.0 0 407 157 155 692.0 0 155 158 231 692.0 0 231 159 60 692.0 0 60 160 258 692.0 0 258 161 383 692.0 0 383 162 404 692.0 0 404 163 62 692.0 0 62 164 44 692.0 0 44 165 47 692.0 0 47 166 35 692.0 0 35 167 56 692.0 0 56 168 48 692.0 0 48 169 39 692.0 0 39 170 36 692.0 0 36 171 32 692.0 0 32 172 38 692.0 0 38 173 38 692.0 0 38 174 32 692.0 0 32 175 58 692.0 0 58 176 59 692.0 0 59 177 87 692.0 0 87 178 36 692.0 0 36 179 47 692.0 0 47 180 54 692.0 0 54 181 95 692.0 0 95 182 90 692.0 0 90 183 81 692.0 0 81 184 156 692.0 0 156 185 58 692.0 0 58 186 132 692.0 0 132 187 72 692.0 0 72 188 80 692.0 0 80 189 72 692.0 0 72 190 248 692.0 0 248 191 38 692.0 0 38 192 37 692.0 0 37 193 98 692.0 0 98 194 42 692.0 0 42 195 54 692.0 0 54 196 57 692.0 0 57 197 39 692.0 0 39 198 37 692.0 0 37 199 34 692.0 0 34 200 45 692.0 0 45 201 44 692.0 0 44 202 86 692.0 0 86 203 66 692.0 0 66 204 63 692.0 0 63 205 69 692.0 0 69 206 81 692.0 0 81 207 74 692.0 0 74 208 202 692.0 0 202 209 92 692.0 0 92 210 55 692.0 0 55 211 61 692.0 0 61 212 322 692.0 0 322 213 124 692.0 0 124 214 107 692.0 0 107 215 54 692.0 0 54 216 56 692.0 0 56 217 38 692.0 0 38 218 48 692.0 0 48 219 82 692.0 0 82 220 174 692.0 0 174 221 129 692.0 0 129 222 110 692.0 0 110 223 86 692.0 0 86 224 70 692.0 0 70 225 81 692.0 0 81 226 111 692.0 0 111 227 47 692.0 0 47 228 77 692.0 0 77 229 71 692.0 0 71 230 36 692.0 0 36 231 69 692.0 0 69 232 71 692.0 0 71 233 123 692.0 0 123 234 87 692.0 0 87 235 214 692.0 0 214 236 98 692.0 0 98 237 156 692.0 0 156 238 55 692.0 0 55 239 99 692.0 0 99 240 79 692.0 0 79 241 82 692.0 0 82 242 86 692.0 0 86 243 122 692.0 0 122 244 81 692.0 0 81 245 87 692.0 0 87 246 73 692.0 0 73 247 59 692.0 0 59 248 75 692.0 0 75 249 91 692.0 0 91 250 92 692.0 0 92 251 116 692.0 0 116 252 172 692.0 0 172 253 218 692.0 0 218 254 453 692.0 0 453 255 1300 692.0 0 1300 256 8818 692.0 0 8818 257 4365 692.0 0 4365 258 6660 692.0 0 6660 259 848 692.0 0 848 260 112 692.0 0 112 261 91 692.0 0 91 262 248 692.0 0 248 263 188 692.0 0 188 264 132 692.0 0 132 265 77 692.0 0 77 266 85 692.0 0 85 267 106 692.0 0 106 268 265 692.0 0 265 269 112 692.0 0 112 270 60 692.0 0 60 271 62 692.0 0 62 272 49 692.0 0 49 273 79 692.0 0 79 274 66 692.0 0 66 275 102 692.0 0 102 276 68 692.0 0 68 277 75 692.0 0 75 278 65 692.0 0 65 279 143 692.0 0 143 280 444 692.0 0 444 281 281 692.0 0 281 282 292 692.0 0 292 283 133 692.0 0 133 284 119 692.0 0 119 285 116 692.0 0 116 286 74 692.0 0 74 287 91 692.0 0 91 288 126 692.0 0 126 289 96 692.0 0 96 290 80 692.0 0 80 291 63 692.0 0 63 292 75 692.0 0 75 293 88 692.0 0 88 294 102 692.0 0 102 295 93 692.0 0 93 296 78 692.0 0 78 297 89 692.0 0 89 298 53 692.0 0 53 299 49 692.0 0 49 300 59 692.0 0 59 301 100 692.0 0 100 Finished in 4.03 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 96,389 Reads with adapters: 14,237 (14.8%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 14,218 (14.8%) Total basepairs processed: 18,725,717 bp Total written (filtered): 3,889,260 bp (20.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14237 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 2 0.0 3 0 0 2 19 173 0.0 3 0 169 4 20 13913 0.0 4 13623 282 8 21 46 0.0 4 12 34 22 8 0.0 4 5 0 3 23 9 0.0 4 1 0 0 7 1 24 1 0.0 4 0 0 0 0 1 28 2 0.0 4 0 0 0 0 2 34 2 0.0 4 0 0 0 0 2 60 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 104 1 0.0 4 1 126 1 0.0 4 0 0 0 0 1 127 61 0.0 4 0 0 0 0 61 141 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 264 5 0.0 4 0 0 0 0 5 265 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.49 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 14,218 Reads with adapters: 375 (2.6%) Reads written (passing filters): 14,218 (100.0%) Total basepairs processed: 3,889,260 bp Total written (filtered): 3,869,124 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 375 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.3% C: 6.7% G: 71.5% T: 11.5% none/other: 6.1% Overview of removed sequences length count expect max.err error counts 3 20 222.2 0 20 4 4 55.5 0 4 5 1 13.9 0 1 9 3 0.1 0 1 2 10 1 0.0 1 0 1 11 264 0.0 1 6 258 12 18 0.0 1 0 18 13 1 0.0 1 0 1 225 5 0.0 1 5 226 1 0.0 1 1 265 1 0.0 1 0 1 266 1 0.0 1 1 271 19 0.0 1 18 1 272 3 0.0 1 3 273 8 0.0 1 8 274 21 0.0 1 20 1 275 4 0.0 1 4