This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.91 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 97,463 (3.4%) Reads that were too short: 23,592 (0.8%) Reads written (passing filters): 73,871 (2.6%) Total basepairs processed: 853,219,115 bp Total written (filtered): 16,606,637 bp (1.9%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 97463 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5533 692.0 0 5533 7 17084 692.0 0 17084 8 1076 692.0 0 1076 9 14585 692.0 0 14585 10 56 692.0 0 56 11 115 692.0 0 115 12 27 692.0 0 27 13 39 692.0 0 39 14 27 692.0 0 27 15 38 692.0 0 38 16 23 692.0 0 23 17 23 692.0 0 23 18 35 692.0 0 35 19 31 692.0 0 31 20 22 692.0 0 22 21 27 692.0 0 27 22 25 692.0 0 25 23 25 692.0 0 25 24 16 692.0 0 16 25 18 692.0 0 18 26 22 692.0 0 22 27 30 692.0 0 30 28 37 692.0 0 37 29 32 692.0 0 32 30 49 692.0 0 49 31 25 692.0 0 25 32 18 692.0 0 18 33 20 692.0 0 20 34 28 692.0 0 28 35 38 692.0 0 38 36 45 692.0 0 45 37 42 692.0 0 42 38 32 692.0 0 32 39 30 692.0 0 30 40 121 692.0 0 121 41 28 692.0 0 28 42 64 692.0 0 64 43 24 692.0 0 24 44 33 692.0 0 33 45 33 692.0 0 33 46 28 692.0 0 28 47 32 692.0 0 32 48 31 692.0 0 31 49 47 692.0 0 47 50 38 692.0 0 38 51 21 692.0 0 21 52 27 692.0 0 27 53 23 692.0 0 23 54 27 692.0 0 27 55 176 692.0 0 176 56 75 692.0 0 75 57 124 692.0 0 124 58 44 692.0 0 44 59 58 692.0 0 58 60 47 692.0 0 47 61 42 692.0 0 42 62 32 692.0 0 32 63 29 692.0 0 29 64 41 692.0 0 41 65 40 692.0 0 40 66 68 692.0 0 68 67 44 692.0 0 44 68 57 692.0 0 57 69 38 692.0 0 38 70 34 692.0 0 34 71 61 692.0 0 61 72 34 692.0 0 34 73 54 692.0 0 54 74 26 692.0 0 26 75 76 692.0 0 76 76 90 692.0 0 90 77 38 692.0 0 38 78 17 692.0 0 17 79 47 692.0 0 47 80 20 692.0 0 20 81 34 692.0 0 34 82 37 692.0 0 37 83 50 692.0 0 50 84 61 692.0 0 61 85 33 692.0 0 33 86 38 692.0 0 38 87 54 692.0 0 54 88 43 692.0 0 43 89 66 692.0 0 66 90 128 692.0 0 128 91 52 692.0 0 52 92 72 692.0 0 72 93 29 692.0 0 29 94 53 692.0 0 53 95 47 692.0 0 47 96 54 692.0 0 54 97 42 692.0 0 42 98 29 692.0 0 29 99 14 692.0 0 14 100 24 692.0 0 24 101 29 692.0 0 29 102 36 692.0 0 36 103 61 692.0 0 61 104 57 692.0 0 57 105 259 692.0 0 259 106 228 692.0 0 228 107 208 692.0 0 208 108 51 692.0 0 51 109 34 692.0 0 34 110 40 692.0 0 40 111 40 692.0 0 40 112 40 692.0 0 40 113 59 692.0 0 59 114 69 692.0 0 69 115 98 692.0 0 98 116 188 692.0 0 188 117 66 692.0 0 66 118 50 692.0 0 50 119 39 692.0 0 39 120 38 692.0 0 38 121 33 692.0 0 33 122 30 692.0 0 30 123 38 692.0 0 38 124 34 692.0 0 34 125 46 692.0 0 46 126 98 692.0 0 98 127 66 692.0 0 66 128 63 692.0 0 63 129 70 692.0 0 70 130 183 692.0 0 183 131 79 692.0 0 79 132 117 692.0 0 117 133 119 692.0 0 119 134 171 692.0 0 171 135 62 692.0 0 62 136 80 692.0 0 80 137 139 692.0 0 139 138 77 692.0 0 77 139 119 692.0 0 119 140 107 692.0 0 107 141 106 692.0 0 106 142 142 692.0 0 142 143 128 692.0 0 128 144 193 692.0 0 193 145 172 692.0 0 172 146 195 692.0 0 195 147 2345 692.0 0 2345 148 1848 692.0 0 1848 149 9763 692.0 0 9763 150 224 692.0 0 224 151 124 692.0 0 124 152 283 692.0 0 283 153 98 692.0 0 98 154 367 692.0 0 367 155 147 692.0 0 147 156 100 692.0 0 100 157 54 692.0 0 54 158 50 692.0 0 50 159 136 692.0 0 136 160 55 692.0 0 55 161 48 692.0 0 48 162 58 692.0 0 58 163 39 692.0 0 39 164 46 692.0 0 46 165 32 692.0 0 32 166 41 692.0 0 41 167 51 692.0 0 51 168 90 692.0 0 90 169 86 692.0 0 86 170 148 692.0 0 148 171 102 692.0 0 102 172 106 692.0 0 106 173 98 692.0 0 98 174 179 692.0 0 179 175 144 692.0 0 144 176 66 692.0 0 66 177 89 692.0 0 89 178 86 692.0 0 86 179 59 692.0 0 59 180 55 692.0 0 55 181 43 692.0 0 43 182 81 692.0 0 81 183 93 692.0 0 93 184 158 692.0 0 158 185 64 692.0 0 64 186 94 692.0 0 94 187 143 692.0 0 143 188 1716 692.0 0 1716 189 821 692.0 0 821 190 1811 692.0 0 1811 191 113 692.0 0 113 192 32 692.0 0 32 193 63 692.0 0 63 194 55 692.0 0 55 195 98 692.0 0 98 196 216 692.0 0 216 197 171 692.0 0 171 198 203 692.0 0 203 199 442 692.0 0 442 200 464 692.0 0 464 201 3194 692.0 0 3194 202 2038 692.0 0 2038 203 7911 692.0 0 7911 204 218 692.0 0 218 205 64 692.0 0 64 206 72 692.0 0 72 207 48 692.0 0 48 208 49 692.0 0 49 209 53 692.0 0 53 210 63 692.0 0 63 211 27 692.0 0 27 212 32 692.0 0 32 213 47 692.0 0 47 214 59 692.0 0 59 215 88 692.0 0 88 216 56 692.0 0 56 217 34 692.0 0 34 218 33 692.0 0 33 219 28 692.0 0 28 220 39 692.0 0 39 221 46 692.0 0 46 222 44 692.0 0 44 223 57 692.0 0 57 224 122 692.0 0 122 225 91 692.0 0 91 226 250 692.0 0 250 227 94 692.0 0 94 228 271 692.0 0 271 229 510 692.0 0 510 230 109 692.0 0 109 231 88 692.0 0 88 232 43 692.0 0 43 233 34 692.0 0 34 234 42 692.0 0 42 235 49 692.0 0 49 236 57 692.0 0 57 237 47 692.0 0 47 238 65 692.0 0 65 239 57 692.0 0 57 240 41 692.0 0 41 241 71 692.0 0 71 242 69 692.0 0 69 243 66 692.0 0 66 244 63 692.0 0 63 245 86 692.0 0 86 246 51 692.0 0 51 247 88 692.0 0 88 248 84 692.0 0 84 249 111 692.0 0 111 250 137 692.0 0 137 251 127 692.0 0 127 252 108 692.0 0 108 253 108 692.0 0 108 254 130 692.0 0 130 255 39 692.0 0 39 256 34 692.0 0 34 257 45 692.0 0 45 258 71 692.0 0 71 259 77 692.0 0 77 260 76 692.0 0 76 261 62 692.0 0 62 262 117 692.0 0 117 263 52 692.0 0 52 264 78 692.0 0 78 265 65 692.0 0 65 266 50 692.0 0 50 267 52 692.0 0 52 268 67 692.0 0 67 269 452 692.0 0 452 270 351 692.0 0 351 271 309 692.0 0 309 272 89 692.0 0 89 273 87 692.0 0 87 274 210 692.0 0 210 275 186 692.0 0 186 276 122 692.0 0 122 277 193 692.0 0 193 278 88 692.0 0 88 279 56 692.0 0 56 280 182 692.0 0 182 281 107 692.0 0 107 282 425 692.0 0 425 283 182 692.0 0 182 284 205 692.0 0 205 285 103 692.0 0 103 286 96 692.0 0 96 287 68 692.0 0 68 288 157 692.0 0 157 289 143 692.0 0 143 290 134 692.0 0 134 291 740 692.0 0 740 292 345 692.0 0 345 293 635 692.0 0 635 294 1119 692.0 0 1119 295 435 692.0 0 435 296 538 692.0 0 538 297 105 692.0 0 105 298 60 692.0 0 60 299 183 692.0 0 183 300 131 692.0 0 131 301 96 692.0 0 96 Finished in 3.12 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 73,871 Reads with adapters: 19,857 (26.9%) Reads that were too short: 121 (0.2%) Reads written (passing filters): 19,736 (26.7%) Total basepairs processed: 16,606,637 bp Total written (filtered): 5,433,296 bp (32.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 19857 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 13 0.0 3 0 0 7 6 16 155 0.0 3 0 124 14 17 17 15278 0.0 3 14058 982 183 55 18 198 0.0 3 44 85 28 41 19 3863 0.0 3 3511 208 70 74 20 38 0.0 3 1 9 2 26 21 3 0.0 3 0 0 0 3 22 40 0.0 3 0 0 0 40 24 1 0.0 3 1 27 2 0.0 3 0 0 0 2 29 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 34 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 41 2 0.0 3 0 0 0 2 42 1 0.0 3 0 0 0 1 43 19 0.0 3 0 0 0 19 44 4 0.0 3 0 0 0 4 45 47 0.0 3 0 0 0 47 46 3 0.0 3 0 0 0 3 47 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 50 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 1 56 3 0.0 3 0 0 0 3 57 3 0.0 3 0 0 0 3 59 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 8 0.0 3 0 0 0 8 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 4 0.0 3 0 0 3 1 79 20 0.0 3 0 0 1 19 80 3 0.0 3 0 0 0 3 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 5 0.0 3 0 1 0 4 86 7 0.0 3 1 0 0 6 87 9 0.0 3 0 0 0 9 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 99 1 0.0 3 0 0 0 1 101 4 0.0 3 0 0 0 4 103 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 1 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 1 118 1 0.0 3 0 0 0 1 119 3 0.0 3 0 0 0 3 120 5 0.0 3 0 0 0 5 121 1 0.0 3 0 0 0 1 122 3 0.0 3 0 0 0 3 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 3 0.0 3 0 0 0 3 133 2 0.0 3 0 0 0 2 134 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 141 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 155 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 168 2 0.0 3 0 0 1 1 170 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 181 3 0.0 3 0 0 0 3 182 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 1 190 1 0.0 3 0 0 0 1 191 3 0.0 3 0 0 0 3 192 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.69 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 19,736 Reads with adapters: 277 (1.4%) Reads written (passing filters): 19,736 (100.0%) Total basepairs processed: 5,433,296 bp Total written (filtered): 5,432,446 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 277 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 17.0% C: 60.3% G: 18.1% T: 4.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 262 308.4 0 262 4 11 77.1 0 11 5 4 19.3 0 4