This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.62 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 92,229 (3.3%) Reads that were too short: 22,249 (0.8%) Reads written (passing filters): 69,980 (2.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 16,056,027 bp (1.9%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 92229 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4883 692.0 0 4883 7 17264 692.0 0 17264 8 746 692.0 0 746 9 16618 692.0 0 16618 10 35 692.0 0 35 11 27 692.0 0 27 12 11 692.0 0 11 13 11 692.0 0 11 14 18 692.0 0 18 15 17 692.0 0 17 16 15 692.0 0 15 17 14 692.0 0 14 18 28 692.0 0 28 19 20 692.0 0 20 20 17 692.0 0 17 21 16 692.0 0 16 22 18 692.0 0 18 23 20 692.0 0 20 24 8 692.0 0 8 25 12 692.0 0 12 26 16 692.0 0 16 27 22 692.0 0 22 28 32 692.0 0 32 29 27 692.0 0 27 30 21 692.0 0 21 31 22 692.0 0 22 32 22 692.0 0 22 33 14 692.0 0 14 34 9 692.0 0 9 35 25 692.0 0 25 36 27 692.0 0 27 37 23 692.0 0 23 38 20 692.0 0 20 39 18 692.0 0 18 40 27 692.0 0 27 41 17 692.0 0 17 42 17 692.0 0 17 43 7 692.0 0 7 44 17 692.0 0 17 45 24 692.0 0 24 46 17 692.0 0 17 47 21 692.0 0 21 48 15 692.0 0 15 49 21 692.0 0 21 50 26 692.0 0 26 51 19 692.0 0 19 52 14 692.0 0 14 53 15 692.0 0 15 54 7 692.0 0 7 55 56 692.0 0 56 56 31 692.0 0 31 57 54 692.0 0 54 58 36 692.0 0 36 59 42 692.0 0 42 60 17 692.0 0 17 61 27 692.0 0 27 62 21 692.0 0 21 63 15 692.0 0 15 64 26 692.0 0 26 65 21 692.0 0 21 66 20 692.0 0 20 67 26 692.0 0 26 68 16 692.0 0 16 69 14 692.0 0 14 70 12 692.0 0 12 71 32 692.0 0 32 72 12 692.0 0 12 73 28 692.0 0 28 74 18 692.0 0 18 75 49 692.0 0 49 76 82 692.0 0 82 77 30 692.0 0 30 78 22 692.0 0 22 79 51 692.0 0 51 80 18 692.0 0 18 81 26 692.0 0 26 82 16 692.0 0 16 83 27 692.0 0 27 84 26 692.0 0 26 85 35 692.0 0 35 86 31 692.0 0 31 87 24 692.0 0 24 88 27 692.0 0 27 89 22 692.0 0 22 90 42 692.0 0 42 91 26 692.0 0 26 92 23 692.0 0 23 93 21 692.0 0 21 94 29 692.0 0 29 95 56 692.0 0 56 96 53 692.0 0 53 97 36 692.0 0 36 98 32 692.0 0 32 99 16 692.0 0 16 100 21 692.0 0 21 101 26 692.0 0 26 102 23 692.0 0 23 103 40 692.0 0 40 104 26 692.0 0 26 105 132 692.0 0 132 106 95 692.0 0 95 107 113 692.0 0 113 108 14 692.0 0 14 109 19 692.0 0 19 110 26 692.0 0 26 111 27 692.0 0 27 112 28 692.0 0 28 113 24 692.0 0 24 114 32 692.0 0 32 115 44 692.0 0 44 116 128 692.0 0 128 117 34 692.0 0 34 118 34 692.0 0 34 119 27 692.0 0 27 120 29 692.0 0 29 121 23 692.0 0 23 122 17 692.0 0 17 123 35 692.0 0 35 124 34 692.0 0 34 125 27 692.0 0 27 126 89 692.0 0 89 127 63 692.0 0 63 128 46 692.0 0 46 129 62 692.0 0 62 130 131 692.0 0 131 131 41 692.0 0 41 132 98 692.0 0 98 133 87 692.0 0 87 134 201 692.0 0 201 135 63 692.0 0 63 136 70 692.0 0 70 137 133 692.0 0 133 138 51 692.0 0 51 139 93 692.0 0 93 140 121 692.0 0 121 141 93 692.0 0 93 142 125 692.0 0 125 143 107 692.0 0 107 144 136 692.0 0 136 145 125 692.0 0 125 146 166 692.0 0 166 147 2263 692.0 0 2263 148 1847 692.0 0 1847 149 10593 692.0 0 10593 150 206 692.0 0 206 151 104 692.0 0 104 152 131 692.0 0 131 153 48 692.0 0 48 154 114 692.0 0 114 155 81 692.0 0 81 156 55 692.0 0 55 157 23 692.0 0 23 158 20 692.0 0 20 159 41 692.0 0 41 160 41 692.0 0 41 161 23 692.0 0 23 162 27 692.0 0 27 163 26 692.0 0 26 164 29 692.0 0 29 165 17 692.0 0 17 166 22 692.0 0 22 167 45 692.0 0 45 168 84 692.0 0 84 169 52 692.0 0 52 170 143 692.0 0 143 171 84 692.0 0 84 172 50 692.0 0 50 173 41 692.0 0 41 174 126 692.0 0 126 175 92 692.0 0 92 176 24 692.0 0 24 177 28 692.0 0 28 178 39 692.0 0 39 179 34 692.0 0 34 180 31 692.0 0 31 181 20 692.0 0 20 182 43 692.0 0 43 183 53 692.0 0 53 184 115 692.0 0 115 185 41 692.0 0 41 186 78 692.0 0 78 187 70 692.0 0 70 188 880 692.0 0 880 189 414 692.0 0 414 190 951 692.0 0 951 191 52 692.0 0 52 192 25 692.0 0 25 193 50 692.0 0 50 194 42 692.0 0 42 195 69 692.0 0 69 196 157 692.0 0 157 197 138 692.0 0 138 198 192 692.0 0 192 199 328 692.0 0 328 200 406 692.0 0 406 201 4389 692.0 0 4389 202 2649 692.0 0 2649 203 13846 692.0 0 13846 204 142 692.0 0 142 205 31 692.0 0 31 206 29 692.0 0 29 207 23 692.0 0 23 208 34 692.0 0 34 209 38 692.0 0 38 210 27 692.0 0 27 211 23 692.0 0 23 212 23 692.0 0 23 213 36 692.0 0 36 214 25 692.0 0 25 215 54 692.0 0 54 216 34 692.0 0 34 217 21 692.0 0 21 218 15 692.0 0 15 219 18 692.0 0 18 220 20 692.0 0 20 221 30 692.0 0 30 222 19 692.0 0 19 223 31 692.0 0 31 224 37 692.0 0 37 225 54 692.0 0 54 226 77 692.0 0 77 227 50 692.0 0 50 228 187 692.0 0 187 229 683 692.0 0 683 230 51 692.0 0 51 231 31 692.0 0 31 232 36 692.0 0 36 233 13 692.0 0 13 234 27 692.0 0 27 235 20 692.0 0 20 236 25 692.0 0 25 237 31 692.0 0 31 238 46 692.0 0 46 239 21 692.0 0 21 240 26 692.0 0 26 241 39 692.0 0 39 242 65 692.0 0 65 243 41 692.0 0 41 244 33 692.0 0 33 245 47 692.0 0 47 246 18 692.0 0 18 247 32 692.0 0 32 248 26 692.0 0 26 249 34 692.0 0 34 250 51 692.0 0 51 251 54 692.0 0 54 252 43 692.0 0 43 253 38 692.0 0 38 254 88 692.0 0 88 255 14 692.0 0 14 256 17 692.0 0 17 257 34 692.0 0 34 258 40 692.0 0 40 259 36 692.0 0 36 260 29 692.0 0 29 261 22 692.0 0 22 262 39 692.0 0 39 263 17 692.0 0 17 264 23 692.0 0 23 265 21 692.0 0 21 266 26 692.0 0 26 267 29 692.0 0 29 268 34 692.0 0 34 269 143 692.0 0 143 270 91 692.0 0 91 271 104 692.0 0 104 272 34 692.0 0 34 273 77 692.0 0 77 274 69 692.0 0 69 275 61 692.0 0 61 276 61 692.0 0 61 277 90 692.0 0 90 278 48 692.0 0 48 279 38 692.0 0 38 280 49 692.0 0 49 281 32 692.0 0 32 282 99 692.0 0 99 283 72 692.0 0 72 284 78 692.0 0 78 285 34 692.0 0 34 286 35 692.0 0 35 287 30 692.0 0 30 288 49 692.0 0 49 289 41 692.0 0 41 290 66 692.0 0 66 291 205 692.0 0 205 292 110 692.0 0 110 293 195 692.0 0 195 294 329 692.0 0 329 295 130 692.0 0 130 296 105 692.0 0 105 297 38 692.0 0 38 298 17 692.0 0 17 299 60 692.0 0 60 300 57 692.0 0 57 301 29 692.0 0 29 Finished in 3.31 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 69,980 Reads with adapters: 21,293 (30.4%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 21,283 (30.4%) Total basepairs processed: 16,056,027 bp Total written (filtered): 5,787,737 bp (36.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 21293 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 13 0.0 3 0 0 13 19 296 0.0 3 0 287 7 0 2 20 17312 0.0 4 16948 346 16 1 1 21 91 0.0 4 18 72 1 22 3517 0.0 4 3455 54 6 2 23 26 0.0 4 1 9 0 15 1 24 2 0.0 4 0 0 0 0 2 25 1 0.0 4 0 0 0 1 29 1 0.0 4 0 0 0 0 1 32 1 0.0 4 1 76 1 0.0 4 1 82 1 0.0 4 0 0 0 0 1 84 2 0.0 4 0 0 2 85 2 0.0 4 1 0 0 1 86 2 0.0 4 0 0 1 1 87 1 0.0 4 0 0 0 1 88 1 0.0 4 1 119 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 127 3 0.0 4 0 0 0 0 3 129 7 0.0 4 0 0 0 0 7 136 1 0.0 4 1 137 1 0.0 4 1 188 1 0.0 4 1 203 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 227 1 0.0 4 1 249 1 0.0 4 0 1 257 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.78 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 21,283 Reads with adapters: 3,762 (17.7%) Reads written (passing filters): 21,283 (100.0%) Total basepairs processed: 5,787,737 bp Total written (filtered): 5,750,833 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3762 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 2.2% G: 95.4% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 332.5 0 8 4 4 83.1 0 4 9 2189 0.1 0 76 2113 10 1293 0.0 1 49 1244 11 251 0.0 1 1 250 12 4 0.0 1 0 4 13 5 0.0 1 0 5 17 1 0.0 1 1 150 2 0.0 1 0 2 208 3 0.0 1 0 3 209 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.