This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.26 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 446,983 (15.8%) Reads that were too short: 45,702 (1.6%) Reads written (passing filters): 401,281 (14.2%) Total basepairs processed: 853,219,115 bp Total written (filtered): 113,244,563 bp (13.3%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 446983 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3921 692.0 0 3921 7 176628 692.0 0 176628 8 4565 692.0 0 4565 9 182623 692.0 0 182623 10 210 692.0 0 210 11 14 692.0 0 14 12 12 692.0 0 12 13 18 692.0 0 18 14 11 692.0 0 11 15 18 692.0 0 18 16 18 692.0 0 18 17 9 692.0 0 9 18 13 692.0 0 13 19 11 692.0 0 11 20 11 692.0 0 11 21 11 692.0 0 11 22 14 692.0 0 14 23 19 692.0 0 19 24 13 692.0 0 13 25 27 692.0 0 27 26 31 692.0 0 31 27 35 692.0 0 35 28 33 692.0 0 33 29 12 692.0 0 12 30 24 692.0 0 24 31 16 692.0 0 16 32 21 692.0 0 21 33 35 692.0 0 35 34 9 692.0 0 9 35 14 692.0 0 14 36 9 692.0 0 9 37 20 692.0 0 20 38 24 692.0 0 24 39 27 692.0 0 27 40 17 692.0 0 17 41 191 692.0 0 191 42 133 692.0 0 133 43 408 692.0 0 408 44 32 692.0 0 32 45 39 692.0 0 39 46 20 692.0 0 20 47 21 692.0 0 21 48 18 692.0 0 18 49 22 692.0 0 22 50 35 692.0 0 35 51 25 692.0 0 25 52 52 692.0 0 52 53 17 692.0 0 17 54 13 692.0 0 13 55 13 692.0 0 13 56 17 692.0 0 17 57 79 692.0 0 79 58 36 692.0 0 36 59 48 692.0 0 48 60 15 692.0 0 15 61 18 692.0 0 18 62 23 692.0 0 23 63 29 692.0 0 29 64 21 692.0 0 21 65 37 692.0 0 37 66 41 692.0 0 41 67 68 692.0 0 68 68 29 692.0 0 29 69 58 692.0 0 58 70 26 692.0 0 26 71 61 692.0 0 61 72 141 692.0 0 141 73 134 692.0 0 134 74 172 692.0 0 172 75 140 692.0 0 140 76 123 692.0 0 123 77 107 692.0 0 107 78 99 692.0 0 99 79 71 692.0 0 71 80 113 692.0 0 113 81 73 692.0 0 73 82 51 692.0 0 51 83 68 692.0 0 68 84 42 692.0 0 42 85 20 692.0 0 20 86 28 692.0 0 28 87 30 692.0 0 30 88 26 692.0 0 26 89 38 692.0 0 38 90 30 692.0 0 30 91 35 692.0 0 35 92 57 692.0 0 57 93 37 692.0 0 37 94 48 692.0 0 48 95 82 692.0 0 82 96 48 692.0 0 48 97 75 692.0 0 75 98 68 692.0 0 68 99 62 692.0 0 62 100 67 692.0 0 67 101 85 692.0 0 85 102 147 692.0 0 147 103 263 692.0 0 263 104 343 692.0 0 343 105 389 692.0 0 389 106 386 692.0 0 386 107 310 692.0 0 310 108 235 692.0 0 235 109 208 692.0 0 208 110 192 692.0 0 192 111 186 692.0 0 186 112 195 692.0 0 195 113 236 692.0 0 236 114 205 692.0 0 205 115 199 692.0 0 199 116 248 692.0 0 248 117 162 692.0 0 162 118 258 692.0 0 258 119 237 692.0 0 237 120 314 692.0 0 314 121 175 692.0 0 175 122 199 692.0 0 199 123 181 692.0 0 181 124 335 692.0 0 335 125 278 692.0 0 278 126 307 692.0 0 307 127 186 692.0 0 186 128 263 692.0 0 263 129 239 692.0 0 239 130 196 692.0 0 196 131 249 692.0 0 249 132 368 692.0 0 368 133 1981 692.0 0 1981 134 1140 692.0 0 1140 135 1796 692.0 0 1796 136 217 692.0 0 217 137 168 692.0 0 168 138 131 692.0 0 131 139 187 692.0 0 187 140 155 692.0 0 155 141 175 692.0 0 175 142 159 692.0 0 159 143 177 692.0 0 177 144 207 692.0 0 207 145 222 692.0 0 222 146 252 692.0 0 252 147 209 692.0 0 209 148 177 692.0 0 177 149 147 692.0 0 147 150 142 692.0 0 142 151 148 692.0 0 148 152 167 692.0 0 167 153 111 692.0 0 111 154 150 692.0 0 150 155 152 692.0 0 152 156 193 692.0 0 193 157 172 692.0 0 172 158 241 692.0 0 241 159 359 692.0 0 359 160 448 692.0 0 448 161 527 692.0 0 527 162 1188 692.0 0 1188 163 642 692.0 0 642 164 943 692.0 0 943 165 320 692.0 0 320 166 240 692.0 0 240 167 247 692.0 0 247 168 194 692.0 0 194 169 193 692.0 0 193 170 187 692.0 0 187 171 218 692.0 0 218 172 263 692.0 0 263 173 233 692.0 0 233 174 156 692.0 0 156 175 157 692.0 0 157 176 139 692.0 0 139 177 133 692.0 0 133 178 180 692.0 0 180 179 183 692.0 0 183 180 271 692.0 0 271 181 197 692.0 0 197 182 241 692.0 0 241 183 482 692.0 0 482 184 290 692.0 0 290 185 259 692.0 0 259 186 208 692.0 0 208 187 253 692.0 0 253 188 213 692.0 0 213 189 226 692.0 0 226 190 278 692.0 0 278 191 285 692.0 0 285 192 249 692.0 0 249 193 257 692.0 0 257 194 195 692.0 0 195 195 162 692.0 0 162 196 160 692.0 0 160 197 153 692.0 0 153 198 201 692.0 0 201 199 170 692.0 0 170 200 205 692.0 0 205 201 203 692.0 0 203 202 226 692.0 0 226 203 233 692.0 0 233 204 171 692.0 0 171 205 205 692.0 0 205 206 227 692.0 0 227 207 299 692.0 0 299 208 273 692.0 0 273 209 294 692.0 0 294 210 276 692.0 0 276 211 444 692.0 0 444 212 379 692.0 0 379 213 2430 692.0 0 2430 214 5004 692.0 0 5004 215 2721 692.0 0 2721 216 719 692.0 0 719 217 331 692.0 0 331 218 290 692.0 0 290 219 313 692.0 0 313 220 314 692.0 0 314 221 345 692.0 0 345 222 299 692.0 0 299 223 336 692.0 0 336 224 430 692.0 0 430 225 1200 692.0 0 1200 226 990 692.0 0 990 227 1043 692.0 0 1043 228 462 692.0 0 462 229 406 692.0 0 406 230 308 692.0 0 308 231 253 692.0 0 253 232 319 692.0 0 319 233 191 692.0 0 191 234 265 692.0 0 265 235 257 692.0 0 257 236 233 692.0 0 233 237 300 692.0 0 300 238 199 692.0 0 199 239 209 692.0 0 209 240 210 692.0 0 210 241 197 692.0 0 197 242 267 692.0 0 267 243 202 692.0 0 202 244 183 692.0 0 183 245 195 692.0 0 195 246 198 692.0 0 198 247 254 692.0 0 254 248 321 692.0 0 321 249 306 692.0 0 306 250 509 692.0 0 509 251 399 692.0 0 399 252 558 692.0 0 558 253 499 692.0 0 499 254 488 692.0 0 488 255 468 692.0 0 468 256 307 692.0 0 307 257 316 692.0 0 316 258 252 692.0 0 252 259 251 692.0 0 251 260 273 692.0 0 273 261 239 692.0 0 239 262 226 692.0 0 226 263 364 692.0 0 364 264 385 692.0 0 385 265 425 692.0 0 425 266 390 692.0 0 390 267 279 692.0 0 279 268 383 692.0 0 383 269 372 692.0 0 372 270 370 692.0 0 370 271 329 692.0 0 329 272 361 692.0 0 361 273 364 692.0 0 364 274 337 692.0 0 337 275 337 692.0 0 337 276 428 692.0 0 428 277 449 692.0 0 449 278 414 692.0 0 414 279 377 692.0 0 377 280 309 692.0 0 309 281 438 692.0 0 438 282 414 692.0 0 414 283 514 692.0 0 514 284 433 692.0 0 433 285 319 692.0 0 319 286 342 692.0 0 342 287 304 692.0 0 304 288 294 692.0 0 294 289 532 692.0 0 532 290 478 692.0 0 478 291 651 692.0 0 651 292 371 692.0 0 371 293 357 692.0 0 357 294 334 692.0 0 334 295 386 692.0 0 386 296 382 692.0 0 382 297 340 692.0 0 340 298 291 692.0 0 291 299 312 692.0 0 312 300 362 692.0 0 362 301 863 692.0 0 863 Finished in 17.51 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 401,281 Reads with adapters: 190,117 (47.4%) Reads that were too short: 281 (0.1%) Reads written (passing filters): 189,836 (47.3%) Total basepairs processed: 113,244,563 bp Total written (filtered): 52,188,393 bp (46.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 190117 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 38 0.0 2 0 0 0 38 15 131 0.0 3 0 0 80 51 16 1694 0.0 3 0 1426 165 103 17 186406 0.0 3 173202 10737 2001 466 18 595 0.0 3 64 428 39 64 19 507 0.0 3 23 15 270 199 20 274 0.0 3 1 2 0 271 21 4 0.0 3 0 0 2 2 23 2 0.0 3 0 0 0 2 24 5 0.0 3 2 0 0 3 25 2 0.0 3 0 0 0 2 26 7 0.0 3 2 0 0 5 27 3 0.0 3 2 0 0 1 28 6 0.0 3 1 0 0 5 29 1 0.0 3 1 30 3 0.0 3 0 0 0 3 31 1 0.0 3 0 0 0 1 32 12 0.0 3 0 0 0 12 34 1 0.0 3 0 0 0 1 35 6 0.0 3 0 0 0 6 37 4 0.0 3 0 0 0 4 38 5 0.0 3 0 0 0 5 39 2 0.0 3 0 0 0 2 41 1 0.0 3 0 0 0 1 43 3 0.0 3 0 0 1 2 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 1 46 2 0.0 3 0 0 0 2 47 4 0.0 3 0 0 0 4 48 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 1 62 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 65 5 0.0 3 0 0 0 5 66 3 0.0 3 0 0 0 3 67 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 1 1 69 2 0.0 3 0 0 0 2 70 2 0.0 3 0 0 0 2 71 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 1 75 2 0.0 3 1 0 0 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 5 0.0 3 0 0 0 5 80 3 0.0 3 0 0 0 3 81 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 85 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 93 3 0.0 3 0 0 0 3 96 2 0.0 3 0 0 0 2 98 2 0.0 3 0 0 0 2 99 2 0.0 3 1 0 0 1 100 2 0.0 3 0 0 0 2 102 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 3 0.0 3 0 0 0 3 111 4 0.0 3 0 0 0 4 112 2 0.0 3 0 0 0 2 113 2 0.0 3 0 0 0 2 115 2 0.0 3 0 0 0 2 116 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 2 0.0 3 0 0 0 2 126 2 0.0 3 0 0 0 2 127 4 0.0 3 0 0 0 4 128 1 0.0 3 0 0 0 1 130 7 0.0 3 0 0 0 7 131 8 0.0 3 0 0 0 8 132 1 0.0 3 0 0 0 1 133 7 0.0 3 0 0 0 7 134 4 0.0 3 0 0 0 4 135 2 0.0 3 0 1 0 1 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 142 2 0.0 3 0 0 0 2 144 2 0.0 3 0 0 0 2 145 2 0.0 3 0 0 0 2 147 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 150 4 0.0 3 0 0 0 4 152 2 0.0 3 0 0 0 2 153 4 0.0 3 0 0 0 4 154 3 0.0 3 0 0 1 2 155 5 0.0 3 0 0 0 5 156 2 0.0 3 0 0 0 2 159 2 0.0 3 0 0 0 2 161 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 7 0.0 3 0 0 0 7 165 1 0.0 3 0 0 0 1 166 4 0.0 3 0 0 0 4 167 1 0.0 3 0 0 0 1 168 2 0.0 3 0 0 1 1 170 1 0.0 3 0 0 0 1 171 2 0.0 3 0 0 0 2 173 1 0.0 3 1 176 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 182 4 0.0 3 0 0 0 4 183 3 0.0 3 0 0 0 3 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 187 4 0.0 3 0 0 0 4 190 3 0.0 3 0 0 0 3 191 3 0.0 3 0 0 0 3 192 4 0.0 3 0 0 0 4 193 1 0.0 3 0 0 0 1 194 8 0.0 3 0 0 0 8 195 2 0.0 3 0 0 0 2 205 4 0.0 3 0 0 0 4 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 2 0.0 3 0 0 0 2 211 1 0.0 3 0 0 0 1 212 1 0.0 3 0 1 214 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 23 0.0 3 0 0 0 23 218 4 0.0 3 0 0 0 4 221 5 0.0 3 0 0 0 5 223 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 237 2 0.0 3 0 0 0 2 238 2 0.0 3 0 0 0 2 239 2 0.0 3 0 0 0 2 242 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 251 2 0.0 3 0 0 0 2 260 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 4 0.0 3 0 0 1 3 275 1 0.0 3 0 0 0 1 278 3 0.0 3 0 0 0 3 279 14 0.0 3 0 0 0 14 280 20 0.0 3 0 0 1 19 281 23 0.0 3 0 0 1 22 282 26 0.0 3 0 0 1 25 283 4 0.0 3 0 0 0 4 285 2 0.0 3 0 0 0 2 289 2 0.0 3 0 0 0 2 292 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.74 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 189,836 Reads with adapters: 9,260 (4.9%) Reads written (passing filters): 189,836 (100.0%) Total basepairs processed: 52,188,393 bp Total written (filtered): 52,160,307 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 9260 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 60.7% C: 14.9% G: 22.2% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 9104 2966.2 0 9104 4 141 741.5 0 141 5 6 185.4 0 6 6 2 46.3 0 2 7 1 11.6 0 1 11 1 0.0 1 0 1 12 3 0.0 1 0 3 13 1 0.0 1 0 1 101 1 0.0 2 0 0 1