This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.50 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 448,955 (15.8%) Reads that were too short: 40,672 (1.4%) Reads written (passing filters): 408,283 (14.4%) Total basepairs processed: 853,219,115 bp Total written (filtered): 115,936,474 bp (13.6%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 448955 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2737 692.0 0 2737 7 199499 692.0 0 199499 8 2411 692.0 0 2411 9 174741 692.0 0 174741 10 115 692.0 0 115 11 11 692.0 0 11 12 10 692.0 0 10 13 10 692.0 0 10 14 16 692.0 0 16 15 15 692.0 0 15 16 17 692.0 0 17 17 16 692.0 0 16 18 13 692.0 0 13 19 4 692.0 0 4 20 11 692.0 0 11 21 13 692.0 0 13 22 11 692.0 0 11 23 12 692.0 0 12 24 10 692.0 0 10 25 8 692.0 0 8 26 13 692.0 0 13 27 13 692.0 0 13 28 28 692.0 0 28 29 20 692.0 0 20 30 19 692.0 0 19 31 25 692.0 0 25 32 19 692.0 0 19 33 23 692.0 0 23 34 11 692.0 0 11 35 13 692.0 0 13 36 10 692.0 0 10 37 17 692.0 0 17 38 11 692.0 0 11 39 29 692.0 0 29 40 27 692.0 0 27 41 148 692.0 0 148 42 60 692.0 0 60 43 173 692.0 0 173 44 33 692.0 0 33 45 35 692.0 0 35 46 18 692.0 0 18 47 22 692.0 0 22 48 17 692.0 0 17 49 22 692.0 0 22 50 26 692.0 0 26 51 23 692.0 0 23 52 42 692.0 0 42 53 10 692.0 0 10 54 14 692.0 0 14 55 9 692.0 0 9 56 12 692.0 0 12 57 45 692.0 0 45 58 18 692.0 0 18 59 19 692.0 0 19 60 24 692.0 0 24 61 11 692.0 0 11 62 20 692.0 0 20 63 15 692.0 0 15 64 12 692.0 0 12 65 17 692.0 0 17 66 19 692.0 0 19 67 52 692.0 0 52 68 19 692.0 0 19 69 73 692.0 0 73 70 29 692.0 0 29 71 48 692.0 0 48 72 51 692.0 0 51 73 48 692.0 0 48 74 56 692.0 0 56 75 59 692.0 0 59 76 105 692.0 0 105 77 196 692.0 0 196 78 185 692.0 0 185 79 163 692.0 0 163 80 142 692.0 0 142 81 141 692.0 0 141 82 169 692.0 0 169 83 204 692.0 0 204 84 185 692.0 0 185 85 101 692.0 0 101 86 84 692.0 0 84 87 74 692.0 0 74 88 73 692.0 0 73 89 84 692.0 0 84 90 83 692.0 0 83 91 105 692.0 0 105 92 82 692.0 0 82 93 83 692.0 0 83 94 80 692.0 0 80 95 78 692.0 0 78 96 56 692.0 0 56 97 60 692.0 0 60 98 78 692.0 0 78 99 78 692.0 0 78 100 71 692.0 0 71 101 81 692.0 0 81 102 90 692.0 0 90 103 156 692.0 0 156 104 194 692.0 0 194 105 221 692.0 0 221 106 223 692.0 0 223 107 238 692.0 0 238 108 162 692.0 0 162 109 174 692.0 0 174 110 159 692.0 0 159 111 169 692.0 0 169 112 147 692.0 0 147 113 235 692.0 0 235 114 168 692.0 0 168 115 186 692.0 0 186 116 203 692.0 0 203 117 141 692.0 0 141 118 214 692.0 0 214 119 187 692.0 0 187 120 266 692.0 0 266 121 166 692.0 0 166 122 196 692.0 0 196 123 183 692.0 0 183 124 254 692.0 0 254 125 250 692.0 0 250 126 266 692.0 0 266 127 167 692.0 0 167 128 261 692.0 0 261 129 220 692.0 0 220 130 199 692.0 0 199 131 216 692.0 0 216 132 255 692.0 0 255 133 1776 692.0 0 1776 134 1099 692.0 0 1099 135 1674 692.0 0 1674 136 178 692.0 0 178 137 158 692.0 0 158 138 147 692.0 0 147 139 148 692.0 0 148 140 177 692.0 0 177 141 183 692.0 0 183 142 140 692.0 0 140 143 181 692.0 0 181 144 189 692.0 0 189 145 165 692.0 0 165 146 181 692.0 0 181 147 166 692.0 0 166 148 168 692.0 0 168 149 147 692.0 0 147 150 144 692.0 0 144 151 131 692.0 0 131 152 148 692.0 0 148 153 154 692.0 0 154 154 175 692.0 0 175 155 156 692.0 0 156 156 138 692.0 0 138 157 159 692.0 0 159 158 176 692.0 0 176 159 222 692.0 0 222 160 300 692.0 0 300 161 286 692.0 0 286 162 697 692.0 0 697 163 399 692.0 0 399 164 585 692.0 0 585 165 209 692.0 0 209 166 163 692.0 0 163 167 141 692.0 0 141 168 140 692.0 0 140 169 152 692.0 0 152 170 156 692.0 0 156 171 175 692.0 0 175 172 236 692.0 0 236 173 184 692.0 0 184 174 163 692.0 0 163 175 176 692.0 0 176 176 117 692.0 0 117 177 115 692.0 0 115 178 162 692.0 0 162 179 218 692.0 0 218 180 200 692.0 0 200 181 139 692.0 0 139 182 220 692.0 0 220 183 456 692.0 0 456 184 258 692.0 0 258 185 241 692.0 0 241 186 204 692.0 0 204 187 233 692.0 0 233 188 218 692.0 0 218 189 254 692.0 0 254 190 210 692.0 0 210 191 227 692.0 0 227 192 231 692.0 0 231 193 195 692.0 0 195 194 163 692.0 0 163 195 131 692.0 0 131 196 170 692.0 0 170 197 192 692.0 0 192 198 186 692.0 0 186 199 193 692.0 0 193 200 222 692.0 0 222 201 222 692.0 0 222 202 220 692.0 0 220 203 216 692.0 0 216 204 176 692.0 0 176 205 179 692.0 0 179 206 176 692.0 0 176 207 229 692.0 0 229 208 209 692.0 0 209 209 287 692.0 0 287 210 260 692.0 0 260 211 557 692.0 0 557 212 473 692.0 0 473 213 3781 692.0 0 3781 214 7020 692.0 0 7020 215 3969 692.0 0 3969 216 471 692.0 0 471 217 243 692.0 0 243 218 284 692.0 0 284 219 255 692.0 0 255 220 238 692.0 0 238 221 261 692.0 0 261 222 281 692.0 0 281 223 214 692.0 0 214 224 272 692.0 0 272 225 575 692.0 0 575 226 565 692.0 0 565 227 541 692.0 0 541 228 321 692.0 0 321 229 309 692.0 0 309 230 296 692.0 0 296 231 189 692.0 0 189 232 260 692.0 0 260 233 193 692.0 0 193 234 208 692.0 0 208 235 204 692.0 0 204 236 196 692.0 0 196 237 243 692.0 0 243 238 191 692.0 0 191 239 185 692.0 0 185 240 203 692.0 0 203 241 196 692.0 0 196 242 169 692.0 0 169 243 208 692.0 0 208 244 156 692.0 0 156 245 194 692.0 0 194 246 189 692.0 0 189 247 247 692.0 0 247 248 242 692.0 0 242 249 300 692.0 0 300 250 416 692.0 0 416 251 332 692.0 0 332 252 460 692.0 0 460 253 280 692.0 0 280 254 282 692.0 0 282 255 221 692.0 0 221 256 236 692.0 0 236 257 232 692.0 0 232 258 208 692.0 0 208 259 210 692.0 0 210 260 228 692.0 0 228 261 209 692.0 0 209 262 202 692.0 0 202 263 206 692.0 0 206 264 252 692.0 0 252 265 246 692.0 0 246 266 235 692.0 0 235 267 188 692.0 0 188 268 207 692.0 0 207 269 224 692.0 0 224 270 231 692.0 0 231 271 257 692.0 0 257 272 201 692.0 0 201 273 246 692.0 0 246 274 283 692.0 0 283 275 249 692.0 0 249 276 291 692.0 0 291 277 305 692.0 0 305 278 306 692.0 0 306 279 283 692.0 0 283 280 230 692.0 0 230 281 311 692.0 0 311 282 359 692.0 0 359 283 281 692.0 0 281 284 283 692.0 0 283 285 264 692.0 0 264 286 287 692.0 0 287 287 254 692.0 0 254 288 206 692.0 0 206 289 366 692.0 0 366 290 283 692.0 0 283 291 348 692.0 0 348 292 236 692.0 0 236 293 221 692.0 0 221 294 232 692.0 0 232 295 248 692.0 0 248 296 295 692.0 0 295 297 244 692.0 0 244 298 242 692.0 0 242 299 214 692.0 0 214 300 246 692.0 0 246 301 445 692.0 0 445 Finished in 19.05 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 408,283 Reads with adapters: 201,050 (49.2%) Reads that were too short: 56 (0.0%) Reads written (passing filters): 200,994 (49.2%) Total basepairs processed: 115,936,474 bp Total written (filtered): 54,966,691 bp (47.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 201050 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 60 0.0 3 0 0 59 1 19 3113 0.0 3 0 3056 50 4 3 20 196314 0.0 4 192686 3478 136 11 3 21 368 0.0 4 65 298 4 0 1 22 21 0.0 4 2 1 15 1 2 23 127 0.0 4 0 0 0 107 20 24 1 0.0 4 1 25 1 0.0 4 1 26 1 0.0 4 0 0 0 1 28 1 0.0 4 1 30 1 0.0 4 0 0 0 0 1 31 1 0.0 4 1 32 1 0.0 4 1 50 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 66 1 0.0 4 1 67 1 0.0 4 0 0 0 0 1 68 1 0.0 4 1 80 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 94 2 0.0 4 1 0 0 0 1 95 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 8 0.0 4 0 0 0 0 8 127 958 0.0 4 0 0 0 0 958 128 4 0.0 4 0 0 0 0 4 130 2 0.0 4 0 0 0 1 1 136 1 0.0 4 1 140 2 0.0 4 0 0 0 1 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 169 1 0.0 4 1 177 1 0.0 4 1 185 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 1 214 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 218 1 0.0 4 1 227 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 230 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 232 2 0.0 4 0 0 0 0 2 233 2 0.0 4 0 0 0 0 2 234 2 0.0 4 0 0 0 0 2 239 1 0.0 4 0 0 0 0 1 257 2 0.0 4 0 0 0 1 1 262 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 277 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 283 2 0.0 4 0 0 0 1 1 284 1 0.0 4 0 0 0 0 1 288 2 0.0 4 0 0 0 0 2 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.89 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 200,994 Reads with adapters: 6,897 (3.4%) Reads written (passing filters): 200,994 (100.0%) Total basepairs processed: 54,966,691 bp Total written (filtered): 54,890,665 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6897 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.9% C: 3.9% G: 89.0% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 347 3140.5 0 347 4 27 785.1 0 27 5 10 196.3 0 10 7 2 12.3 0 2 8 1 3.1 0 1 9 15 0.8 0 5 10 10 38 0.2 1 1 37 11 6157 0.0 1 62 6095 12 257 0.0 1 5 252 13 9 0.0 1 0 9 14 2 0.0 1 0 2 15 8 0.0 1 0 8 16 3 0.0 1 0 3 20 2 0.0 1 2 40 1 0.0 1 1 124 3 0.0 1 3 152 4 0.0 1 0 4 155 1 0.0 1 1 156 1 0.0 1 1 171 1 0.0 1 1 172 2 0.0 1 2 210 5 0.0 1 3 2 230 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.