This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 72.98 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 663,756 (23.4%) Reads that were too short: 592,697 (20.9%) Reads written (passing filters): 71,059 (2.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 17,665,044 bp (2.1%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 663756 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 545 692.0 0 545 7 1273 692.0 0 1273 8 25991 692.0 0 25991 9 21174 692.0 0 21174 10 70 692.0 0 70 11 35 692.0 0 35 12 33 692.0 0 33 13 56 692.0 0 56 14 58 692.0 0 58 15 25 692.0 0 25 16 44 692.0 0 44 17 33 692.0 0 33 18 69 692.0 0 69 19 45 692.0 0 45 20 119 692.0 0 119 21 54 692.0 0 54 22 106 692.0 0 106 23 43 692.0 0 43 24 49 692.0 0 49 25 45 692.0 0 45 26 59 692.0 0 59 27 48 692.0 0 48 28 49 692.0 0 49 29 37 692.0 0 37 30 28 692.0 0 28 31 39 692.0 0 39 32 42 692.0 0 42 33 138 692.0 0 138 34 55 692.0 0 55 35 63 692.0 0 63 36 76 692.0 0 76 37 82 692.0 0 82 38 55 692.0 0 55 39 27 692.0 0 27 40 29 692.0 0 29 41 12 692.0 0 12 42 40 692.0 0 40 43 39 692.0 0 39 44 46 692.0 0 46 45 36 692.0 0 36 46 33 692.0 0 33 47 57 692.0 0 57 48 36 692.0 0 36 49 65 692.0 0 65 50 37 692.0 0 37 51 44 692.0 0 44 52 28 692.0 0 28 53 37 692.0 0 37 54 35 692.0 0 35 55 23 692.0 0 23 56 25 692.0 0 25 57 43 692.0 0 43 58 24 692.0 0 24 59 27 692.0 0 27 60 43 692.0 0 43 61 34 692.0 0 34 62 31 692.0 0 31 63 69 692.0 0 69 64 62 692.0 0 62 65 39 692.0 0 39 66 62 692.0 0 62 67 56 692.0 0 56 68 44 692.0 0 44 69 33 692.0 0 33 70 22 692.0 0 22 71 35 692.0 0 35 72 35 692.0 0 35 73 111 692.0 0 111 74 72 692.0 0 72 75 40 692.0 0 40 76 49 692.0 0 49 77 62 692.0 0 62 78 47 692.0 0 47 79 41 692.0 0 41 80 40 692.0 0 40 81 35 692.0 0 35 82 46 692.0 0 46 83 29 692.0 0 29 84 55 692.0 0 55 85 50 692.0 0 50 86 37 692.0 0 37 87 58 692.0 0 58 88 69 692.0 0 69 89 89 692.0 0 89 90 61 692.0 0 61 91 86 692.0 0 86 92 107 692.0 0 107 93 81 692.0 0 81 94 82 692.0 0 82 95 120 692.0 0 120 96 46 692.0 0 46 97 75 692.0 0 75 98 53 692.0 0 53 99 79 692.0 0 79 100 46 692.0 0 46 101 40 692.0 0 40 102 30 692.0 0 30 103 38 692.0 0 38 104 33 692.0 0 33 105 36 692.0 0 36 106 30 692.0 0 30 107 50 692.0 0 50 108 39 692.0 0 39 109 29 692.0 0 29 110 36 692.0 0 36 111 34 692.0 0 34 112 35 692.0 0 35 113 46 692.0 0 46 114 34 692.0 0 34 115 29 692.0 0 29 116 59 692.0 0 59 117 34 692.0 0 34 118 48 692.0 0 48 119 44 692.0 0 44 120 47 692.0 0 47 121 55 692.0 0 55 122 73 692.0 0 73 123 47 692.0 0 47 124 154 692.0 0 154 125 236 692.0 0 236 126 219 692.0 0 219 127 241 692.0 0 241 128 171 692.0 0 171 129 48 692.0 0 48 130 68 692.0 0 68 131 264 692.0 0 264 132 503 692.0 0 503 133 189 692.0 0 189 134 126 692.0 0 126 135 66 692.0 0 66 136 47 692.0 0 47 137 42 692.0 0 42 138 45 692.0 0 45 139 39 692.0 0 39 140 44 692.0 0 44 141 39 692.0 0 39 142 41 692.0 0 41 143 43 692.0 0 43 144 61 692.0 0 61 145 58 692.0 0 58 146 92 692.0 0 92 147 99 692.0 0 99 148 73 692.0 0 73 149 35 692.0 0 35 150 45 692.0 0 45 151 39 692.0 0 39 152 48 692.0 0 48 153 53 692.0 0 53 154 66 692.0 0 66 155 106 692.0 0 106 156 100 692.0 0 100 157 32 692.0 0 32 158 36 692.0 0 36 159 35 692.0 0 35 160 44 692.0 0 44 161 33 692.0 0 33 162 27 692.0 0 27 163 37 692.0 0 37 164 40 692.0 0 40 165 46 692.0 0 46 166 69 692.0 0 69 167 60 692.0 0 60 168 44 692.0 0 44 169 32 692.0 0 32 170 33 692.0 0 33 171 30 692.0 0 30 172 53 692.0 0 53 173 83 692.0 0 83 174 196 692.0 0 196 175 207 692.0 0 207 176 214 692.0 0 214 177 59 692.0 0 59 178 74 692.0 0 74 179 222 692.0 0 222 180 168 692.0 0 168 181 365 692.0 0 365 182 107 692.0 0 107 183 77 692.0 0 77 184 57 692.0 0 57 185 241 692.0 0 241 186 152 692.0 0 152 187 307 692.0 0 307 188 93 692.0 0 93 189 86 692.0 0 86 190 101 692.0 0 101 191 146 692.0 0 146 192 425 692.0 0 425 193 3022 692.0 0 3022 194 1125 692.0 0 1125 195 1808 692.0 0 1808 196 121 692.0 0 121 197 106 692.0 0 106 198 112 692.0 0 112 199 465 692.0 0 465 200 760 692.0 0 760 201 1351 692.0 0 1351 202 303 692.0 0 303 203 91 692.0 0 91 204 46 692.0 0 46 205 91 692.0 0 91 206 207 692.0 0 207 207 143 692.0 0 143 208 99 692.0 0 99 209 63 692.0 0 63 210 96 692.0 0 96 211 130 692.0 0 130 212 146 692.0 0 146 213 225 692.0 0 225 214 116 692.0 0 116 215 124 692.0 0 124 216 113 692.0 0 113 217 113 692.0 0 113 218 157 692.0 0 157 219 292 692.0 0 292 220 324 692.0 0 324 221 482 692.0 0 482 222 639 692.0 0 639 223 811 692.0 0 811 224 1261 692.0 0 1261 225 420 692.0 0 420 226 362 692.0 0 362 227 648 692.0 0 648 228 377 692.0 0 377 229 449 692.0 0 449 230 690 692.0 0 690 231 951 692.0 0 951 232 2551 692.0 0 2551 233 2592 692.0 0 2592 234 8418 692.0 0 8418 235 1992 692.0 0 1992 236 2033 692.0 0 2033 237 39324 692.0 0 39324 238 12006 692.0 0 12006 239 41771 692.0 0 41771 240 1756 692.0 0 1756 241 1867 692.0 0 1867 242 709 692.0 0 709 243 1238 692.0 0 1238 244 1824 692.0 0 1824 245 895 692.0 0 895 246 792 692.0 0 792 247 1127 692.0 0 1127 248 847 692.0 0 847 249 1061 692.0 0 1061 250 1871 692.0 0 1871 251 1698 692.0 0 1698 252 2558 692.0 0 2558 253 2462 692.0 0 2462 254 3962 692.0 0 3962 255 4019 692.0 0 4019 256 5268 692.0 0 5268 257 107027 692.0 0 107027 258 71737 692.0 0 71737 259 244905 692.0 0 244905 260 9211 692.0 0 9211 261 128 692.0 0 128 262 122 692.0 0 122 263 45 692.0 0 45 264 35 692.0 0 35 265 31 692.0 0 31 266 62 692.0 0 62 267 60 692.0 0 60 268 200 692.0 0 200 269 255 692.0 0 255 270 114 692.0 0 114 271 62 692.0 0 62 272 105 692.0 0 105 273 57 692.0 0 57 274 53 692.0 0 53 275 73 692.0 0 73 276 63 692.0 0 63 277 40 692.0 0 40 278 43 692.0 0 43 279 74 692.0 0 74 280 56 692.0 0 56 281 68 692.0 0 68 282 112 692.0 0 112 283 60 692.0 0 60 284 80 692.0 0 80 285 99 692.0 0 99 286 93 692.0 0 93 287 188 692.0 0 188 288 103 692.0 0 103 289 212 692.0 0 212 290 216 692.0 0 216 291 661 692.0 0 661 292 381 692.0 0 381 293 411 692.0 0 411 294 127 692.0 0 127 295 108 692.0 0 108 296 107 692.0 0 107 297 95 692.0 0 95 298 41 692.0 0 41 299 49 692.0 0 49 300 166 692.0 0 166 301 152 692.0 0 152 Finished in 3.23 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 71,059 Reads with adapters: 25,323 (35.6%) Reads that were too short: 135 (0.2%) Reads written (passing filters): 25,188 (35.4%) Total basepairs processed: 17,665,044 bp Total written (filtered): 6,940,886 bp (39.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 25323 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 13 0.0 3 0 0 9 4 16 226 0.0 3 0 184 26 16 17 24432 0.0 3 22642 1474 256 60 18 233 0.0 3 5 59 1 168 19 118 0.0 3 2 0 75 41 20 96 0.0 3 7 0 1 88 22 2 0.0 3 0 0 0 2 23 1 0.0 3 0 0 0 1 24 2 0.0 3 0 0 0 2 31 3 0.0 3 0 0 0 3 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 37 3 0.0 3 0 0 0 3 38 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 43 4 0.0 3 0 0 0 4 44 2 0.0 3 0 0 0 2 45 2 0.0 3 0 0 0 2 46 1 0.0 3 0 0 0 1 47 17 0.0 3 0 0 0 17 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 60 21 0.0 3 0 0 0 21 63 2 0.0 3 0 0 0 2 64 3 0.0 3 0 0 0 3 69 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 1 78 2 0.0 3 0 0 0 2 79 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 2 0.0 3 0 1 0 1 92 1 0.0 3 0 0 0 1 93 3 0.0 3 0 0 0 3 95 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 102 2 0.0 3 0 0 0 2 103 3 0.0 3 0 0 1 2 104 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 1 117 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 132 4 0.0 3 0 0 0 4 137 1 0.0 3 0 0 1 138 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 160 3 0.0 3 0 0 0 3 162 1 0.0 3 0 0 0 1 163 3 0.0 3 0 0 0 3 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 28 0.0 3 0 0 1 27 181 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 5 0.0 3 0 0 1 4 205 2 0.0 3 0 0 0 2 207 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 224 1 0.0 3 0 0 0 1 225 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 2 0.0 3 0 0 0 2 286 1 0.0 3 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.88 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 25,188 Reads with adapters: 222 (0.9%) Reads written (passing filters): 25,188 (100.0%) Total basepairs processed: 6,940,886 bp Total written (filtered): 6,940,168 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 222 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 32.9% C: 14.9% G: 45.0% T: 7.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 184 393.6 0 184 4 33 98.4 0 33 5 1 24.6 0 1 6 2 6.1 0 2 7 1 1.5 0 1 10 1 0.0 1 0 1