This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 72.59 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 670,338 (23.6%) Reads that were too short: 598,991 (21.1%) Reads written (passing filters): 71,347 (2.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 18,089,901 bp (2.1%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 670338 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 561 692.0 0 561 7 1027 692.0 0 1027 8 25269 692.0 0 25269 9 23933 692.0 0 23933 10 75 692.0 0 75 11 52 692.0 0 52 12 46 692.0 0 46 13 45 692.0 0 45 14 58 692.0 0 58 15 28 692.0 0 28 16 52 692.0 0 52 17 59 692.0 0 59 18 72 692.0 0 72 19 44 692.0 0 44 20 72 692.0 0 72 21 33 692.0 0 33 22 75 692.0 0 75 23 60 692.0 0 60 24 53 692.0 0 53 25 62 692.0 0 62 26 72 692.0 0 72 27 39 692.0 0 39 28 48 692.0 0 48 29 47 692.0 0 47 30 22 692.0 0 22 31 42 692.0 0 42 32 36 692.0 0 36 33 43 692.0 0 43 34 41 692.0 0 41 35 53 692.0 0 53 36 58 692.0 0 58 37 86 692.0 0 86 38 56 692.0 0 56 39 37 692.0 0 37 40 40 692.0 0 40 41 35 692.0 0 35 42 36 692.0 0 36 43 32 692.0 0 32 44 46 692.0 0 46 45 38 692.0 0 38 46 40 692.0 0 40 47 63 692.0 0 63 48 37 692.0 0 37 49 60 692.0 0 60 50 60 692.0 0 60 51 59 692.0 0 59 52 35 692.0 0 35 53 50 692.0 0 50 54 40 692.0 0 40 55 38 692.0 0 38 56 35 692.0 0 35 57 57 692.0 0 57 58 27 692.0 0 27 59 39 692.0 0 39 60 38 692.0 0 38 61 48 692.0 0 48 62 33 692.0 0 33 63 83 692.0 0 83 64 84 692.0 0 84 65 28 692.0 0 28 66 53 692.0 0 53 67 86 692.0 0 86 68 44 692.0 0 44 69 51 692.0 0 51 70 28 692.0 0 28 71 55 692.0 0 55 72 50 692.0 0 50 73 138 692.0 0 138 74 48 692.0 0 48 75 55 692.0 0 55 76 50 692.0 0 50 77 65 692.0 0 65 78 50 692.0 0 50 79 51 692.0 0 51 80 32 692.0 0 32 81 45 692.0 0 45 82 50 692.0 0 50 83 42 692.0 0 42 84 67 692.0 0 67 85 55 692.0 0 55 86 58 692.0 0 58 87 59 692.0 0 59 88 65 692.0 0 65 89 97 692.0 0 97 90 71 692.0 0 71 91 106 692.0 0 106 92 123 692.0 0 123 93 62 692.0 0 62 94 70 692.0 0 70 95 54 692.0 0 54 96 27 692.0 0 27 97 57 692.0 0 57 98 49 692.0 0 49 99 55 692.0 0 55 100 45 692.0 0 45 101 32 692.0 0 32 102 52 692.0 0 52 103 40 692.0 0 40 104 40 692.0 0 40 105 39 692.0 0 39 106 57 692.0 0 57 107 41 692.0 0 41 108 47 692.0 0 47 109 39 692.0 0 39 110 46 692.0 0 46 111 28 692.0 0 28 112 36 692.0 0 36 113 48 692.0 0 48 114 39 692.0 0 39 115 42 692.0 0 42 116 66 692.0 0 66 117 49 692.0 0 49 118 69 692.0 0 69 119 50 692.0 0 50 120 45 692.0 0 45 121 55 692.0 0 55 122 69 692.0 0 69 123 66 692.0 0 66 124 212 692.0 0 212 125 133 692.0 0 133 126 325 692.0 0 325 127 94 692.0 0 94 128 62 692.0 0 62 129 53 692.0 0 53 130 75 692.0 0 75 131 238 692.0 0 238 132 570 692.0 0 570 133 189 692.0 0 189 134 93 692.0 0 93 135 56 692.0 0 56 136 62 692.0 0 62 137 45 692.0 0 45 138 48 692.0 0 48 139 61 692.0 0 61 140 70 692.0 0 70 141 63 692.0 0 63 142 63 692.0 0 63 143 59 692.0 0 59 144 82 692.0 0 82 145 99 692.0 0 99 146 98 692.0 0 98 147 102 692.0 0 102 148 122 692.0 0 122 149 60 692.0 0 60 150 70 692.0 0 70 151 65 692.0 0 65 152 77 692.0 0 77 153 74 692.0 0 74 154 99 692.0 0 99 155 112 692.0 0 112 156 91 692.0 0 91 157 69 692.0 0 69 158 63 692.0 0 63 159 59 692.0 0 59 160 69 692.0 0 69 161 71 692.0 0 71 162 80 692.0 0 80 163 72 692.0 0 72 164 73 692.0 0 73 165 88 692.0 0 88 166 90 692.0 0 90 167 112 692.0 0 112 168 90 692.0 0 90 169 71 692.0 0 71 170 77 692.0 0 77 171 88 692.0 0 88 172 101 692.0 0 101 173 102 692.0 0 102 174 193 692.0 0 193 175 205 692.0 0 205 176 174 692.0 0 174 177 81 692.0 0 81 178 92 692.0 0 92 179 188 692.0 0 188 180 140 692.0 0 140 181 210 692.0 0 210 182 121 692.0 0 121 183 88 692.0 0 88 184 96 692.0 0 96 185 273 692.0 0 273 186 187 692.0 0 187 187 311 692.0 0 311 188 130 692.0 0 130 189 126 692.0 0 126 190 134 692.0 0 134 191 155 692.0 0 155 192 287 692.0 0 287 193 1954 692.0 0 1954 194 1052 692.0 0 1052 195 1592 692.0 0 1592 196 141 692.0 0 141 197 136 692.0 0 136 198 101 692.0 0 101 199 345 692.0 0 345 200 392 692.0 0 392 201 601 692.0 0 601 202 194 692.0 0 194 203 83 692.0 0 83 204 87 692.0 0 87 205 126 692.0 0 126 206 158 692.0 0 158 207 144 692.0 0 144 208 130 692.0 0 130 209 109 692.0 0 109 210 127 692.0 0 127 211 168 692.0 0 168 212 139 692.0 0 139 213 255 692.0 0 255 214 153 692.0 0 153 215 195 692.0 0 195 216 172 692.0 0 172 217 187 692.0 0 187 218 204 692.0 0 204 219 360 692.0 0 360 220 313 692.0 0 313 221 415 692.0 0 415 222 621 692.0 0 621 223 888 692.0 0 888 224 1498 692.0 0 1498 225 506 692.0 0 506 226 494 692.0 0 494 227 733 692.0 0 733 228 467 692.0 0 467 229 458 692.0 0 458 230 746 692.0 0 746 231 1026 692.0 0 1026 232 2925 692.0 0 2925 233 2989 692.0 0 2989 234 10537 692.0 0 10537 235 2038 692.0 0 2038 236 1956 692.0 0 1956 237 41417 692.0 0 41417 238 12466 692.0 0 12466 239 48984 692.0 0 48984 240 1524 692.0 0 1524 241 1964 692.0 0 1964 242 819 692.0 0 819 243 1334 692.0 0 1334 244 2208 692.0 0 2208 245 1188 692.0 0 1188 246 936 692.0 0 936 247 1395 692.0 0 1395 248 1031 692.0 0 1031 249 1307 692.0 0 1307 250 2017 692.0 0 2017 251 1948 692.0 0 1948 252 2817 692.0 0 2817 253 2794 692.0 0 2794 254 4343 692.0 0 4343 255 3872 692.0 0 3872 256 4784 692.0 0 4784 257 102436 692.0 0 102436 258 75663 692.0 0 75663 259 230547 692.0 0 230547 260 13200 692.0 0 13200 261 148 692.0 0 148 262 112 692.0 0 112 263 85 692.0 0 85 264 81 692.0 0 81 265 86 692.0 0 86 266 91 692.0 0 91 267 88 692.0 0 88 268 234 692.0 0 234 269 314 692.0 0 314 270 123 692.0 0 123 271 83 692.0 0 83 272 83 692.0 0 83 273 91 692.0 0 91 274 76 692.0 0 76 275 82 692.0 0 82 276 89 692.0 0 89 277 67 692.0 0 67 278 78 692.0 0 78 279 92 692.0 0 92 280 99 692.0 0 99 281 87 692.0 0 87 282 125 692.0 0 125 283 89 692.0 0 89 284 86 692.0 0 86 285 84 692.0 0 84 286 78 692.0 0 78 287 131 692.0 0 131 288 97 692.0 0 97 289 173 692.0 0 173 290 233 692.0 0 233 291 884 692.0 0 884 292 640 692.0 0 640 293 556 692.0 0 556 294 167 692.0 0 167 295 121 692.0 0 121 296 109 692.0 0 109 297 103 692.0 0 103 298 77 692.0 0 77 299 70 692.0 0 70 300 119 692.0 0 119 301 165 692.0 0 165 Finished in 3.62 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 71,347 Reads with adapters: 26,238 (36.8%) Reads that were too short: 21 (0.0%) Reads written (passing filters): 26,217 (36.7%) Total basepairs processed: 18,089,901 bp Total written (filtered): 7,114,332 bp (39.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 26238 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 15 0.0 3 0 0 0 1 14 18 17 0.0 3 0 0 16 1 19 607 0.0 3 0 596 10 0 1 20 25314 0.0 4 24817 473 19 4 1 21 54 0.0 4 5 46 2 1 22 11 0.0 4 4 0 5 0 2 23 16 0.0 4 1 0 0 12 3 24 1 0.0 4 0 0 0 0 1 41 2 0.0 4 0 0 0 0 2 52 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 101 1 0.0 4 1 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 174 0.0 4 0 0 0 0 174 133 1 0.0 4 1 135 5 0.0 4 4 0 0 0 1 136 1 0.0 4 1 137 1 0.0 4 0 0 0 1 138 1 0.0 4 1 201 1 0.0 4 0 1 219 2 0.0 4 0 0 0 0 2 228 2 0.0 4 0 0 0 0 2 246 1 0.0 4 0 0 0 0 1 250 1 0.0 4 1 251 2 0.0 4 1 0 0 0 1 260 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.93 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 26,217 Reads with adapters: 4,011 (15.3%) Reads written (passing filters): 26,217 (100.0%) Total basepairs processed: 7,114,332 bp Total written (filtered): 7,074,833 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4011 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 3.1% G: 94.4% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 409.6 0 6 4 4 102.4 0 4 6 1 6.4 0 1 9 1935 0.1 0 51 1884 10 1734 0.0 1 59 1675 11 319 0.0 1 4 315 12 3 0.0 1 0 3 13 2 0.0 1 0 2 138 4 0.0 1 3 1 150 1 0.0 1 0 1 208 1 0.0 1 0 1 223 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.