This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 164.31 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 41,900 (1.2%) Reads that were too short: 9,816 (0.3%) Reads written (passing filters): 32,084 (0.9%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 5,906,155 bp (0.6%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 41900 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 41 847.3 0 41 7 24 847.3 0 24 8 14 847.3 0 14 9 22 847.3 0 22 10 21 847.3 0 21 11 28 847.3 0 28 12 25 847.3 0 25 13 37 847.3 0 37 14 23 847.3 0 23 15 24 847.3 0 24 16 30 847.3 0 30 17 29 847.3 0 29 18 19 847.3 0 19 19 13 847.3 0 13 20 16 847.3 0 16 21 16 847.3 0 16 22 27 847.3 0 27 23 19 847.3 0 19 24 26 847.3 0 26 25 40 847.3 0 40 26 110 847.3 0 110 27 82 847.3 0 82 28 83 847.3 0 83 29 85 847.3 0 85 30 125 847.3 0 125 31 75 847.3 0 75 32 16 847.3 0 16 33 29 847.3 0 29 34 32 847.3 0 32 35 37 847.3 0 37 36 35 847.3 0 35 37 32 847.3 0 32 38 31 847.3 0 31 39 34 847.3 0 34 40 42 847.3 0 42 41 11 847.3 0 11 42 54 847.3 0 54 43 37 847.3 0 37 44 22 847.3 0 22 45 23 847.3 0 23 46 19 847.3 0 19 47 17 847.3 0 17 48 22 847.3 0 22 49 40 847.3 0 40 50 32 847.3 0 32 51 42 847.3 0 42 52 43 847.3 0 43 53 43 847.3 0 43 54 38 847.3 0 38 55 34 847.3 0 34 56 45 847.3 0 45 57 56 847.3 0 56 58 59 847.3 0 59 59 44 847.3 0 44 60 35 847.3 0 35 61 34 847.3 0 34 62 44 847.3 0 44 63 73 847.3 0 73 64 69 847.3 0 69 65 72 847.3 0 72 66 60 847.3 0 60 67 102 847.3 0 102 68 106 847.3 0 106 69 716 847.3 0 716 70 3993 847.3 0 3993 71 1925 847.3 0 1925 72 41 847.3 0 41 73 37 847.3 0 37 74 42 847.3 0 42 75 49 847.3 0 49 76 49 847.3 0 49 77 92 847.3 0 92 78 708 847.3 0 708 79 95 847.3 0 95 80 29 847.3 0 29 81 41 847.3 0 41 82 41 847.3 0 41 83 47 847.3 0 47 84 41 847.3 0 41 85 48 847.3 0 48 86 52 847.3 0 52 87 31 847.3 0 31 88 30 847.3 0 30 89 44 847.3 0 44 90 57 847.3 0 57 91 35 847.3 0 35 92 44 847.3 0 44 93 34 847.3 0 34 94 66 847.3 0 66 95 43 847.3 0 43 96 62 847.3 0 62 97 80 847.3 0 80 98 62 847.3 0 62 99 60 847.3 0 60 100 53 847.3 0 53 101 48 847.3 0 48 102 37 847.3 0 37 103 30 847.3 0 30 104 27 847.3 0 27 105 39 847.3 0 39 106 23 847.3 0 23 107 35 847.3 0 35 108 36 847.3 0 36 109 42 847.3 0 42 110 46 847.3 0 46 111 54 847.3 0 54 112 66 847.3 0 66 113 55 847.3 0 55 114 444 847.3 0 444 115 155 847.3 0 155 116 77 847.3 0 77 117 43 847.3 0 43 118 49 847.3 0 49 119 30 847.3 0 30 120 36 847.3 0 36 121 45 847.3 0 45 122 49 847.3 0 49 123 58 847.3 0 58 124 69 847.3 0 69 125 84 847.3 0 84 126 55 847.3 0 55 127 74 847.3 0 74 128 78 847.3 0 78 129 121 847.3 0 121 130 96 847.3 0 96 131 127 847.3 0 127 132 185 847.3 0 185 133 89 847.3 0 89 134 133 847.3 0 133 135 107 847.3 0 107 136 110 847.3 0 110 137 282 847.3 0 282 138 2435 847.3 0 2435 139 2101 847.3 0 2101 140 1822 847.3 0 1822 141 334 847.3 0 334 142 94 847.3 0 94 143 1023 847.3 0 1023 144 974 847.3 0 974 145 515 847.3 0 515 146 69 847.3 0 69 147 83 847.3 0 83 148 570 847.3 0 570 149 1634 847.3 0 1634 150 1913 847.3 0 1913 151 1119 847.3 0 1119 152 574 847.3 0 574 153 59 847.3 0 59 154 62 847.3 0 62 155 43 847.3 0 43 156 31 847.3 0 31 157 38 847.3 0 38 158 34 847.3 0 34 159 66 847.3 0 66 160 26 847.3 0 26 161 35 847.3 0 35 162 33 847.3 0 33 163 22 847.3 0 22 164 28 847.3 0 28 165 27 847.3 0 27 166 26 847.3 0 26 167 23 847.3 0 23 168 26 847.3 0 26 169 31 847.3 0 31 170 42 847.3 0 42 171 25 847.3 0 25 172 45 847.3 0 45 173 37 847.3 0 37 174 29 847.3 0 29 175 46 847.3 0 46 176 42 847.3 0 42 177 30 847.3 0 30 178 34 847.3 0 34 179 34 847.3 0 34 180 27 847.3 0 27 181 31 847.3 0 31 182 36 847.3 0 36 183 33 847.3 0 33 184 72 847.3 0 72 185 52 847.3 0 52 186 50 847.3 0 50 187 43 847.3 0 43 188 68 847.3 0 68 189 139 847.3 0 139 190 126 847.3 0 126 191 211 847.3 0 211 192 90 847.3 0 90 193 41 847.3 0 41 194 58 847.3 0 58 195 40 847.3 0 40 196 35 847.3 0 35 197 36 847.3 0 36 198 52 847.3 0 52 199 32 847.3 0 32 200 28 847.3 0 28 201 51 847.3 0 51 202 33 847.3 0 33 203 54 847.3 0 54 204 32 847.3 0 32 205 38 847.3 0 38 206 32 847.3 0 32 207 37 847.3 0 37 208 31 847.3 0 31 209 164 847.3 0 164 210 108 847.3 0 108 211 69 847.3 0 69 212 41 847.3 0 41 213 50 847.3 0 50 214 54 847.3 0 54 215 49 847.3 0 49 216 35 847.3 0 35 217 49 847.3 0 49 218 32 847.3 0 32 219 36 847.3 0 36 220 49 847.3 0 49 221 42 847.3 0 42 222 61 847.3 0 61 223 46 847.3 0 46 224 27 847.3 0 27 225 39 847.3 0 39 226 49 847.3 0 49 227 29 847.3 0 29 228 40 847.3 0 40 229 35 847.3 0 35 230 30 847.3 0 30 231 42 847.3 0 42 232 55 847.3 0 55 233 107 847.3 0 107 234 156 847.3 0 156 235 181 847.3 0 181 236 74 847.3 0 74 237 42 847.3 0 42 238 65 847.3 0 65 239 44 847.3 0 44 240 49 847.3 0 49 241 43 847.3 0 43 242 52 847.3 0 52 243 63 847.3 0 63 244 54 847.3 0 54 245 72 847.3 0 72 246 69 847.3 0 69 247 105 847.3 0 105 248 126 847.3 0 126 249 168 847.3 0 168 250 125 847.3 0 125 251 238 847.3 0 238 252 110 847.3 0 110 253 337 847.3 0 337 254 398 847.3 0 398 255 231 847.3 0 231 256 53 847.3 0 53 257 56 847.3 0 56 258 56 847.3 0 56 259 44 847.3 0 44 260 35 847.3 0 35 261 46 847.3 0 46 262 38 847.3 0 38 263 30 847.3 0 30 264 43 847.3 0 43 265 45 847.3 0 45 266 50 847.3 0 50 267 48 847.3 0 48 268 103 847.3 0 103 269 75 847.3 0 75 270 68 847.3 0 68 271 41 847.3 0 41 272 67 847.3 0 67 273 210 847.3 0 210 274 228 847.3 0 228 275 93 847.3 0 93 276 47 847.3 0 47 277 45 847.3 0 45 278 61 847.3 0 61 279 52 847.3 0 52 280 100 847.3 0 100 281 119 847.3 0 119 282 101 847.3 0 101 283 78 847.3 0 78 284 82 847.3 0 82 285 147 847.3 0 147 286 130 847.3 0 130 287 172 847.3 0 172 288 173 847.3 0 173 289 223 847.3 0 223 290 99 847.3 0 99 291 116 847.3 0 116 292 180 847.3 0 180 293 143 847.3 0 143 294 162 847.3 0 162 295 177 847.3 0 177 296 173 847.3 0 173 297 165 847.3 0 165 298 188 847.3 0 188 299 229 847.3 0 229 300 320 847.3 0 320 301 508 847.3 0 508 Finished in 1.96 s (61 us/read; 0.98 M reads/minute). === Summary === Total reads processed: 32,084 Reads with adapters: 37 (0.1%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 29 (0.1%) Total basepairs processed: 5,906,155 bp Total written (filtered): 6,922 bp (0.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 37 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 20 1 0.0 4 1 21 19 0.0 4 17 1 0 0 1 22 2 0.0 4 2 26 1 0.0 4 1 108 3 0.0 4 0 0 0 0 3 109 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 158 1 0.0 4 1 160 1 0.0 4 0 0 1 161 1 0.0 4 1 214 1 0.0 4 0 0 0 1 221 1 0.0 4 0 0 0 1 230 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (345 us/read; 0.17 M reads/minute). === Summary === Total reads processed: 29 Reads with adapters: 2 (6.9%) Reads written (passing filters): 29 (100.0%) Total basepairs processed: 6,922 bp Total written (filtered): 6,909 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 50.0% C: 0.0% G: 50.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 0.5 0 1 10 1 0.0 1 0 1