This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 165.88 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 126,444 (3.6%) Reads that were too short: 22,913 (0.7%) Reads written (passing filters): 103,531 (3.0%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 28,273,662 bp (2.7%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 126444 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4416 847.3 0 4416 7 1343 847.3 0 1343 8 42104 847.3 0 42104 9 34998 847.3 0 34998 10 149 847.3 0 149 11 4 847.3 0 4 12 1 847.3 0 1 13 8 847.3 0 8 14 4 847.3 0 4 15 7 847.3 0 7 16 4 847.3 0 4 17 7 847.3 0 7 18 6 847.3 0 6 19 4 847.3 0 4 20 5 847.3 0 5 21 1 847.3 0 1 22 7 847.3 0 7 23 16 847.3 0 16 24 7 847.3 0 7 25 6 847.3 0 6 26 13 847.3 0 13 27 21 847.3 0 21 28 14 847.3 0 14 29 12 847.3 0 12 30 7 847.3 0 7 31 2 847.3 0 2 32 4 847.3 0 4 33 24 847.3 0 24 34 9 847.3 0 9 35 8 847.3 0 8 36 6 847.3 0 6 37 7 847.3 0 7 38 17 847.3 0 17 39 13 847.3 0 13 40 15 847.3 0 15 41 13 847.3 0 13 42 28 847.3 0 28 43 43 847.3 0 43 44 10 847.3 0 10 45 11 847.3 0 11 46 8 847.3 0 8 47 24 847.3 0 24 48 11 847.3 0 11 49 14 847.3 0 14 50 10 847.3 0 10 51 11 847.3 0 11 52 20 847.3 0 20 53 20 847.3 0 20 54 37 847.3 0 37 55 15 847.3 0 15 56 11 847.3 0 11 57 53 847.3 0 53 58 130 847.3 0 130 59 125 847.3 0 125 60 1069 847.3 0 1069 61 1495 847.3 0 1495 62 2414 847.3 0 2414 63 24 847.3 0 24 64 19 847.3 0 19 65 29 847.3 0 29 66 132 847.3 0 132 67 377 847.3 0 377 68 107 847.3 0 107 69 10 847.3 0 10 70 11 847.3 0 11 71 15 847.3 0 15 72 22 847.3 0 22 73 71 847.3 0 71 74 12 847.3 0 12 75 25 847.3 0 25 76 28 847.3 0 28 77 294 847.3 0 294 78 272 847.3 0 272 79 479 847.3 0 479 80 48 847.3 0 48 81 37 847.3 0 37 82 193 847.3 0 193 83 76 847.3 0 76 84 32 847.3 0 32 85 47 847.3 0 47 86 41 847.3 0 41 87 8 847.3 0 8 88 14 847.3 0 14 89 61 847.3 0 61 90 31 847.3 0 31 91 34 847.3 0 34 92 67 847.3 0 67 93 38 847.3 0 38 94 39 847.3 0 39 95 40 847.3 0 40 96 7 847.3 0 7 97 39 847.3 0 39 98 28 847.3 0 28 99 33 847.3 0 33 100 43 847.3 0 43 101 73 847.3 0 73 102 19 847.3 0 19 103 23 847.3 0 23 104 34 847.3 0 34 105 32 847.3 0 32 106 26 847.3 0 26 107 55 847.3 0 55 108 49 847.3 0 49 109 44 847.3 0 44 110 48 847.3 0 48 111 110 847.3 0 110 112 165 847.3 0 165 113 145 847.3 0 145 114 1317 847.3 0 1317 115 3571 847.3 0 3571 116 700 847.3 0 700 117 76 847.3 0 76 118 29 847.3 0 29 119 32 847.3 0 32 120 21 847.3 0 21 121 24 847.3 0 24 122 39 847.3 0 39 123 30 847.3 0 30 124 39 847.3 0 39 125 56 847.3 0 56 126 32 847.3 0 32 127 47 847.3 0 47 128 28 847.3 0 28 129 41 847.3 0 41 130 48 847.3 0 48 131 56 847.3 0 56 132 60 847.3 0 60 133 43 847.3 0 43 134 23 847.3 0 23 135 33 847.3 0 33 136 50 847.3 0 50 137 47 847.3 0 47 138 61 847.3 0 61 139 45 847.3 0 45 140 34 847.3 0 34 141 43 847.3 0 43 142 40 847.3 0 40 143 78 847.3 0 78 144 72 847.3 0 72 145 73 847.3 0 73 146 47 847.3 0 47 147 56 847.3 0 56 148 46 847.3 0 46 149 75 847.3 0 75 150 23 847.3 0 23 151 22 847.3 0 22 152 29 847.3 0 29 153 34 847.3 0 34 154 40 847.3 0 40 155 41 847.3 0 41 156 29 847.3 0 29 157 33 847.3 0 33 158 32 847.3 0 32 159 37 847.3 0 37 160 32 847.3 0 32 161 41 847.3 0 41 162 38 847.3 0 38 163 68 847.3 0 68 164 79 847.3 0 79 165 309 847.3 0 309 166 193 847.3 0 193 167 141 847.3 0 141 168 42 847.3 0 42 169 34 847.3 0 34 170 36 847.3 0 36 171 39 847.3 0 39 172 37 847.3 0 37 173 51 847.3 0 51 174 34 847.3 0 34 175 32 847.3 0 32 176 34 847.3 0 34 177 50 847.3 0 50 178 47 847.3 0 47 179 59 847.3 0 59 180 85 847.3 0 85 181 273 847.3 0 273 182 37 847.3 0 37 183 32 847.3 0 32 184 74 847.3 0 74 185 104 847.3 0 104 186 61 847.3 0 61 187 53 847.3 0 53 188 58 847.3 0 58 189 108 847.3 0 108 190 130 847.3 0 130 191 215 847.3 0 215 192 355 847.3 0 355 193 104 847.3 0 104 194 26 847.3 0 26 195 58 847.3 0 58 196 56 847.3 0 56 197 78 847.3 0 78 198 92 847.3 0 92 199 54 847.3 0 54 200 54 847.3 0 54 201 49 847.3 0 49 202 45 847.3 0 45 203 144 847.3 0 144 204 591 847.3 0 591 205 140 847.3 0 140 206 42 847.3 0 42 207 105 847.3 0 105 208 144 847.3 0 144 209 86 847.3 0 86 210 89 847.3 0 89 211 52 847.3 0 52 212 90 847.3 0 90 213 133 847.3 0 133 214 226 847.3 0 226 215 85 847.3 0 85 216 118 847.3 0 118 217 133 847.3 0 133 218 132 847.3 0 132 219 108 847.3 0 108 220 38 847.3 0 38 221 31 847.3 0 31 222 38 847.3 0 38 223 45 847.3 0 45 224 94 847.3 0 94 225 121 847.3 0 121 226 139 847.3 0 139 227 68 847.3 0 68 228 64 847.3 0 64 229 72 847.3 0 72 230 54 847.3 0 54 231 81 847.3 0 81 232 144 847.3 0 144 233 116 847.3 0 116 234 103 847.3 0 103 235 149 847.3 0 149 236 343 847.3 0 343 237 494 847.3 0 494 238 628 847.3 0 628 239 844 847.3 0 844 240 106 847.3 0 106 241 109 847.3 0 109 242 159 847.3 0 159 243 190 847.3 0 190 244 327 847.3 0 327 245 5439 847.3 0 5439 246 3236 847.3 0 3236 247 1304 847.3 0 1304 248 86 847.3 0 86 249 45 847.3 0 45 250 63 847.3 0 63 251 64 847.3 0 64 252 55 847.3 0 55 253 60 847.3 0 60 254 121 847.3 0 121 255 110 847.3 0 110 256 67 847.3 0 67 257 58 847.3 0 58 258 59 847.3 0 59 259 130 847.3 0 130 260 123 847.3 0 123 261 117 847.3 0 117 262 185 847.3 0 185 263 134 847.3 0 134 264 174 847.3 0 174 265 390 847.3 0 390 266 329 847.3 0 329 267 196 847.3 0 196 268 46 847.3 0 46 269 32 847.3 0 32 270 199 847.3 0 199 271 223 847.3 0 223 272 219 847.3 0 219 273 69 847.3 0 69 274 42 847.3 0 42 275 47 847.3 0 47 276 84 847.3 0 84 277 134 847.3 0 134 278 142 847.3 0 142 279 138 847.3 0 138 280 86 847.3 0 86 281 50 847.3 0 50 282 53 847.3 0 53 283 68 847.3 0 68 284 82 847.3 0 82 285 122 847.3 0 122 286 118 847.3 0 118 287 91 847.3 0 91 288 69 847.3 0 69 289 69 847.3 0 69 290 59 847.3 0 59 291 85 847.3 0 85 292 97 847.3 0 97 293 102 847.3 0 102 294 93 847.3 0 93 295 110 847.3 0 110 296 67 847.3 0 67 297 121 847.3 0 121 298 118 847.3 0 118 299 107 847.3 0 107 300 116 847.3 0 116 301 90 847.3 0 90 Finished in 7.62 s (74 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 103,531 Reads with adapters: 42,267 (40.8%) Reads that were too short: 118 (0.1%) Reads written (passing filters): 42,149 (40.7%) Total basepairs processed: 28,273,662 bp Total written (filtered): 11,616,100 bp (41.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 42267 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 25 0.0 3 0 0 11 14 16 514 0.0 3 0 448 45 21 17 41160 0.0 3 38863 1921 310 66 18 157 0.0 3 32 84 12 29 19 112 0.0 3 4 0 65 43 20 52 0.0 3 0 0 0 52 21 2 0.0 3 0 0 0 2 22 2 0.0 3 1 0 0 1 26 11 0.0 3 0 0 0 11 28 4 0.0 3 0 0 0 4 29 4 0.0 3 0 0 0 4 30 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 35 1 0.0 3 1 36 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 1 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 57 4 0.0 3 0 0 1 3 60 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 1 0.0 3 1 76 1 0.0 3 0 0 0 1 77 7 0.0 3 0 0 0 7 78 43 0.0 3 0 0 0 43 80 3 0.0 3 0 0 0 3 81 6 0.0 3 0 0 0 6 82 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 1 122 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 131 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 139 3 0.0 3 0 0 0 3 140 2 0.0 3 0 0 1 1 141 2 0.0 3 0 0 0 2 148 2 0.0 3 0 0 0 2 151 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 1 158 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 207 3 0.0 3 0 0 0 3 210 1 0.0 3 0 0 0 1 216 4 0.0 3 0 0 1 3 217 49 0.0 3 0 0 4 45 226 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 229 4 0.0 3 0 0 0 4 235 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 4 0.0 3 0 0 0 4 282 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 284 2 0.0 3 0 0 0 2 285 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.47 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 42,149 Reads with adapters: 671 (1.6%) Reads written (passing filters): 42,149 (100.0%) Total basepairs processed: 11,616,100 bp Total written (filtered): 11,613,804 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 671 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 39.0% C: 7.7% G: 50.2% T: 3.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 402 658.6 0 402 4 263 164.6 0 263 5 5 41.2 0 5 13 1 0.0 1 0 1