This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 167.36 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 120,047 (3.5%) Reads that were too short: 19,679 (0.6%) Reads written (passing filters): 100,368 (2.9%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 27,946,062 bp (2.7%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 120047 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4948 847.3 0 4948 7 1044 847.3 0 1044 8 39290 847.3 0 39290 9 41000 847.3 0 41000 10 66 847.3 0 66 11 1 847.3 0 1 12 2 847.3 0 2 13 6 847.3 0 6 14 2 847.3 0 2 15 6 847.3 0 6 16 4 847.3 0 4 17 2 847.3 0 2 18 4 847.3 0 4 19 1 847.3 0 1 20 4 847.3 0 4 21 4 847.3 0 4 22 2 847.3 0 2 23 8 847.3 0 8 24 7 847.3 0 7 25 1 847.3 0 1 26 3 847.3 0 3 27 7 847.3 0 7 28 4 847.3 0 4 29 10 847.3 0 10 30 5 847.3 0 5 31 3 847.3 0 3 32 4 847.3 0 4 33 12 847.3 0 12 34 5 847.3 0 5 35 6 847.3 0 6 36 6 847.3 0 6 37 6 847.3 0 6 38 1 847.3 0 1 39 14 847.3 0 14 40 18 847.3 0 18 41 7 847.3 0 7 42 13 847.3 0 13 43 16 847.3 0 16 44 7 847.3 0 7 45 3 847.3 0 3 46 11 847.3 0 11 47 7 847.3 0 7 48 16 847.3 0 16 49 12 847.3 0 12 50 20 847.3 0 20 51 9 847.3 0 9 52 17 847.3 0 17 53 15 847.3 0 15 54 12 847.3 0 12 55 13 847.3 0 13 56 12 847.3 0 12 57 43 847.3 0 43 58 78 847.3 0 78 59 74 847.3 0 74 60 661 847.3 0 661 61 843 847.3 0 843 62 1651 847.3 0 1651 63 15 847.3 0 15 64 20 847.3 0 20 65 17 847.3 0 17 66 69 847.3 0 69 67 371 847.3 0 371 68 70 847.3 0 70 69 6 847.3 0 6 70 7 847.3 0 7 71 8 847.3 0 8 72 7 847.3 0 7 73 37 847.3 0 37 74 6 847.3 0 6 75 9 847.3 0 9 76 11 847.3 0 11 77 157 847.3 0 157 78 176 847.3 0 176 79 395 847.3 0 395 80 14 847.3 0 14 81 25 847.3 0 25 82 32 847.3 0 32 83 20 847.3 0 20 84 29 847.3 0 29 85 25 847.3 0 25 86 45 847.3 0 45 87 9 847.3 0 9 88 17 847.3 0 17 89 29 847.3 0 29 90 33 847.3 0 33 91 21 847.3 0 21 92 19 847.3 0 19 93 41 847.3 0 41 94 49 847.3 0 49 95 28 847.3 0 28 96 23 847.3 0 23 97 44 847.3 0 44 98 37 847.3 0 37 99 38 847.3 0 38 100 28 847.3 0 28 101 55 847.3 0 55 102 16 847.3 0 16 103 29 847.3 0 29 104 32 847.3 0 32 105 28 847.3 0 28 106 23 847.3 0 23 107 54 847.3 0 54 108 49 847.3 0 49 109 30 847.3 0 30 110 37 847.3 0 37 111 79 847.3 0 79 112 124 847.3 0 124 113 86 847.3 0 86 114 639 847.3 0 639 115 2673 847.3 0 2673 116 360 847.3 0 360 117 54 847.3 0 54 118 14 847.3 0 14 119 20 847.3 0 20 120 16 847.3 0 16 121 18 847.3 0 18 122 23 847.3 0 23 123 26 847.3 0 26 124 35 847.3 0 35 125 35 847.3 0 35 126 30 847.3 0 30 127 39 847.3 0 39 128 13 847.3 0 13 129 31 847.3 0 31 130 36 847.3 0 36 131 34 847.3 0 34 132 29 847.3 0 29 133 18 847.3 0 18 134 22 847.3 0 22 135 26 847.3 0 26 136 25 847.3 0 25 137 39 847.3 0 39 138 34 847.3 0 34 139 30 847.3 0 30 140 40 847.3 0 40 141 21 847.3 0 21 142 21 847.3 0 21 143 43 847.3 0 43 144 44 847.3 0 44 145 50 847.3 0 50 146 27 847.3 0 27 147 32 847.3 0 32 148 40 847.3 0 40 149 73 847.3 0 73 150 22 847.3 0 22 151 26 847.3 0 26 152 17 847.3 0 17 153 33 847.3 0 33 154 24 847.3 0 24 155 21 847.3 0 21 156 17 847.3 0 17 157 18 847.3 0 18 158 21 847.3 0 21 159 39 847.3 0 39 160 20 847.3 0 20 161 36 847.3 0 36 162 28 847.3 0 28 163 35 847.3 0 35 164 37 847.3 0 37 165 163 847.3 0 163 166 154 847.3 0 154 167 83 847.3 0 83 168 23 847.3 0 23 169 21 847.3 0 21 170 24 847.3 0 24 171 22 847.3 0 22 172 27 847.3 0 27 173 19 847.3 0 19 174 25 847.3 0 25 175 24 847.3 0 24 176 23 847.3 0 23 177 35 847.3 0 35 178 32 847.3 0 32 179 60 847.3 0 60 180 56 847.3 0 56 181 368 847.3 0 368 182 32 847.3 0 32 183 21 847.3 0 21 184 42 847.3 0 42 185 66 847.3 0 66 186 42 847.3 0 42 187 43 847.3 0 43 188 26 847.3 0 26 189 88 847.3 0 88 190 105 847.3 0 105 191 187 847.3 0 187 192 358 847.3 0 358 193 95 847.3 0 95 194 29 847.3 0 29 195 51 847.3 0 51 196 38 847.3 0 38 197 46 847.3 0 46 198 49 847.3 0 49 199 36 847.3 0 36 200 37 847.3 0 37 201 39 847.3 0 39 202 40 847.3 0 40 203 133 847.3 0 133 204 681 847.3 0 681 205 141 847.3 0 141 206 29 847.3 0 29 207 81 847.3 0 81 208 87 847.3 0 87 209 51 847.3 0 51 210 80 847.3 0 80 211 29 847.3 0 29 212 77 847.3 0 77 213 215 847.3 0 215 214 386 847.3 0 386 215 52 847.3 0 52 216 60 847.3 0 60 217 67 847.3 0 67 218 62 847.3 0 62 219 49 847.3 0 49 220 30 847.3 0 30 221 31 847.3 0 31 222 23 847.3 0 23 223 36 847.3 0 36 224 36 847.3 0 36 225 46 847.3 0 46 226 55 847.3 0 55 227 44 847.3 0 44 228 49 847.3 0 49 229 36 847.3 0 36 230 39 847.3 0 39 231 68 847.3 0 68 232 92 847.3 0 92 233 70 847.3 0 70 234 89 847.3 0 89 235 103 847.3 0 103 236 141 847.3 0 141 237 285 847.3 0 285 238 350 847.3 0 350 239 412 847.3 0 412 240 79 847.3 0 79 241 81 847.3 0 81 242 106 847.3 0 106 243 164 847.3 0 164 244 270 847.3 0 270 245 5798 847.3 0 5798 246 3574 847.3 0 3574 247 1253 847.3 0 1253 248 49 847.3 0 49 249 34 847.3 0 34 250 39 847.3 0 39 251 42 847.3 0 42 252 42 847.3 0 42 253 47 847.3 0 47 254 65 847.3 0 65 255 82 847.3 0 82 256 54 847.3 0 54 257 26 847.3 0 26 258 28 847.3 0 28 259 62 847.3 0 62 260 89 847.3 0 89 261 108 847.3 0 108 262 163 847.3 0 163 263 72 847.3 0 72 264 88 847.3 0 88 265 451 847.3 0 451 266 304 847.3 0 304 267 208 847.3 0 208 268 35 847.3 0 35 269 20 847.3 0 20 270 81 847.3 0 81 271 112 847.3 0 112 272 114 847.3 0 114 273 35 847.3 0 35 274 23 847.3 0 23 275 39 847.3 0 39 276 58 847.3 0 58 277 94 847.3 0 94 278 81 847.3 0 81 279 55 847.3 0 55 280 35 847.3 0 35 281 26 847.3 0 26 282 39 847.3 0 39 283 37 847.3 0 37 284 41 847.3 0 41 285 76 847.3 0 76 286 68 847.3 0 68 287 42 847.3 0 42 288 44 847.3 0 44 289 44 847.3 0 44 290 36 847.3 0 36 291 56 847.3 0 56 292 88 847.3 0 88 293 52 847.3 0 52 294 58 847.3 0 58 295 76 847.3 0 76 296 55 847.3 0 55 297 80 847.3 0 80 298 59 847.3 0 59 299 65 847.3 0 65 300 71 847.3 0 71 301 51 847.3 0 51 Finished in 8.03 s (80 us/read; 0.75 M reads/minute). === Summary === Total reads processed: 100,368 Reads with adapters: 47,900 (47.7%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 47,886 (47.7%) Total basepairs processed: 27,946,062 bp Total written (filtered): 12,951,549 bp (46.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 47900 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 0 0 2 17 2 0.0 3 0 0 0 0 2 18 26 0.0 3 0 0 26 19 736 0.0 3 0 724 10 0 2 20 41866 0.0 4 41099 743 20 4 21 198 0.0 4 66 125 7 22 4241 0.0 4 4165 66 10 23 39 0.0 4 2 8 0 22 7 24 7 0.0 4 4 0 1 0 2 25 4 0.0 4 0 0 0 4 27 1 0.0 4 1 52 1 0.0 4 0 0 0 0 1 93 1 0.0 4 1 118 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 8 0.0 4 0 0 0 0 8 127 721 0.0 4 0 0 0 0 721 128 27 0.0 4 0 0 0 1 26 138 1 0.0 4 1 145 1 0.0 4 0 1 153 1 0.0 4 0 0 0 0 1 157 1 0.0 4 1 158 1 0.0 4 1 165 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 217 2 0.0 4 0 0 0 0 2 228 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 1 264 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.84 s (59 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 47,886 Reads with adapters: 7,476 (15.6%) Reads written (passing filters): 47,886 (100.0%) Total basepairs processed: 12,951,549 bp Total written (filtered): 12,879,019 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7476 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.6% C: 2.0% G: 96.5% T: 0.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 748.2 0 4 4 9 187.1 0 9 5 5 46.8 0 5 6 1 11.7 0 1 7 1 2.9 0 1 8 2 0.7 0 2 9 4923 0.2 0 116 4807 10 2013 0.0 1 67 1946 11 494 0.0 1 8 486 12 9 0.0 1 0 9 19 1 0.0 1 1 150 7 0.0 1 4 3 153 2 0.0 1 0 2 208 2 0.0 1 2 210 1 0.0 1 1 223 1 0.0 1 1 225 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.