This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 166.54 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 310,552 (8.9%) Reads that were too short: 99,467 (2.9%) Reads written (passing filters): 211,085 (6.1%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 58,160,398 bp (5.6%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 310552 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2067 847.3 0 2067 7 95764 847.3 0 95764 8 81534 847.3 0 81534 9 256 847.3 0 256 10 100 847.3 0 100 11 104 847.3 0 104 12 55 847.3 0 55 13 82 847.3 0 82 14 63 847.3 0 63 15 70 847.3 0 70 16 80 847.3 0 80 17 120 847.3 0 120 18 52 847.3 0 52 19 59 847.3 0 59 20 56 847.3 0 56 21 29 847.3 0 29 22 50 847.3 0 50 23 64 847.3 0 64 24 84 847.3 0 84 25 66 847.3 0 66 26 57 847.3 0 57 27 53 847.3 0 53 28 39 847.3 0 39 29 68 847.3 0 68 30 66 847.3 0 66 31 66 847.3 0 66 32 75 847.3 0 75 33 58 847.3 0 58 34 69 847.3 0 69 35 61 847.3 0 61 36 73 847.3 0 73 37 57 847.3 0 57 38 83 847.3 0 83 39 47 847.3 0 47 40 64 847.3 0 64 41 83 847.3 0 83 42 91 847.3 0 91 43 113 847.3 0 113 44 72 847.3 0 72 45 75 847.3 0 75 46 71 847.3 0 71 47 76 847.3 0 76 48 79 847.3 0 79 49 66 847.3 0 66 50 66 847.3 0 66 51 95 847.3 0 95 52 133 847.3 0 133 53 156 847.3 0 156 54 119 847.3 0 119 55 83 847.3 0 83 56 76 847.3 0 76 57 82 847.3 0 82 58 86 847.3 0 86 59 120 847.3 0 120 60 226 847.3 0 226 61 147 847.3 0 147 62 80 847.3 0 80 63 93 847.3 0 93 64 102 847.3 0 102 65 75 847.3 0 75 66 98 847.3 0 98 67 85 847.3 0 85 68 94 847.3 0 94 69 89 847.3 0 89 70 103 847.3 0 103 71 87 847.3 0 87 72 246 847.3 0 246 73 275 847.3 0 275 74 325 847.3 0 325 75 125 847.3 0 125 76 97 847.3 0 97 77 104 847.3 0 104 78 123 847.3 0 123 79 134 847.3 0 134 80 140 847.3 0 140 81 112 847.3 0 112 82 120 847.3 0 120 83 104 847.3 0 104 84 122 847.3 0 122 85 96 847.3 0 96 86 125 847.3 0 125 87 83 847.3 0 83 88 92 847.3 0 92 89 111 847.3 0 111 90 83 847.3 0 83 91 82 847.3 0 82 92 79 847.3 0 79 93 100 847.3 0 100 94 109 847.3 0 109 95 107 847.3 0 107 96 79 847.3 0 79 97 74 847.3 0 74 98 82 847.3 0 82 99 100 847.3 0 100 100 93 847.3 0 93 101 101 847.3 0 101 102 154 847.3 0 154 103 254 847.3 0 254 104 260 847.3 0 260 105 167 847.3 0 167 106 235 847.3 0 235 107 208 847.3 0 208 108 154 847.3 0 154 109 150 847.3 0 150 110 156 847.3 0 156 111 180 847.3 0 180 112 144 847.3 0 144 113 147 847.3 0 147 114 156 847.3 0 156 115 153 847.3 0 153 116 192 847.3 0 192 117 168 847.3 0 168 118 142 847.3 0 142 119 142 847.3 0 142 120 156 847.3 0 156 121 215 847.3 0 215 122 208 847.3 0 208 123 152 847.3 0 152 124 456 847.3 0 456 125 348 847.3 0 348 126 326 847.3 0 326 127 147 847.3 0 147 128 129 847.3 0 129 129 138 847.3 0 138 130 129 847.3 0 129 131 181 847.3 0 181 132 208 847.3 0 208 133 276 847.3 0 276 134 267 847.3 0 267 135 260 847.3 0 260 136 161 847.3 0 161 137 194 847.3 0 194 138 164 847.3 0 164 139 161 847.3 0 161 140 144 847.3 0 144 141 172 847.3 0 172 142 143 847.3 0 143 143 177 847.3 0 177 144 161 847.3 0 161 145 219 847.3 0 219 146 245 847.3 0 245 147 231 847.3 0 231 148 260 847.3 0 260 149 412 847.3 0 412 150 312 847.3 0 312 151 231 847.3 0 231 152 221 847.3 0 221 153 226 847.3 0 226 154 215 847.3 0 215 155 193 847.3 0 193 156 224 847.3 0 224 157 199 847.3 0 199 158 289 847.3 0 289 159 282 847.3 0 282 160 255 847.3 0 255 161 249 847.3 0 249 162 290 847.3 0 290 163 221 847.3 0 221 164 209 847.3 0 209 165 225 847.3 0 225 166 259 847.3 0 259 167 242 847.3 0 242 168 215 847.3 0 215 169 411 847.3 0 411 170 466 847.3 0 466 171 571 847.3 0 571 172 438 847.3 0 438 173 245 847.3 0 245 174 245 847.3 0 245 175 197 847.3 0 197 176 151 847.3 0 151 177 151 847.3 0 151 178 144 847.3 0 144 179 167 847.3 0 167 180 280 847.3 0 280 181 263 847.3 0 263 182 226 847.3 0 226 183 251 847.3 0 251 184 246 847.3 0 246 185 290 847.3 0 290 186 226 847.3 0 226 187 414 847.3 0 414 188 398 847.3 0 398 189 329 847.3 0 329 190 234 847.3 0 234 191 201 847.3 0 201 192 199 847.3 0 199 193 227 847.3 0 227 194 177 847.3 0 177 195 213 847.3 0 213 196 191 847.3 0 191 197 169 847.3 0 169 198 184 847.3 0 184 199 218 847.3 0 218 200 256 847.3 0 256 201 213 847.3 0 213 202 206 847.3 0 206 203 324 847.3 0 324 204 357 847.3 0 357 205 452 847.3 0 452 206 184 847.3 0 184 207 172 847.3 0 172 208 175 847.3 0 175 209 234 847.3 0 234 210 225 847.3 0 225 211 205 847.3 0 205 212 239 847.3 0 239 213 306 847.3 0 306 214 630 847.3 0 630 215 1471 847.3 0 1471 216 1685 847.3 0 1685 217 1970 847.3 0 1970 218 329 847.3 0 329 219 371 847.3 0 371 220 296 847.3 0 296 221 341 847.3 0 341 222 344 847.3 0 344 223 474 847.3 0 474 224 516 847.3 0 516 225 831 847.3 0 831 226 3714 847.3 0 3714 227 7073 847.3 0 7073 228 9046 847.3 0 9046 229 17004 847.3 0 17004 230 1786 847.3 0 1786 231 1688 847.3 0 1688 232 1495 847.3 0 1495 233 2560 847.3 0 2560 234 189 847.3 0 189 235 151 847.3 0 151 236 182 847.3 0 182 237 271 847.3 0 271 238 329 847.3 0 329 239 438 847.3 0 438 240 367 847.3 0 367 241 189 847.3 0 189 242 171 847.3 0 171 243 178 847.3 0 178 244 199 847.3 0 199 245 172 847.3 0 172 246 192 847.3 0 192 247 231 847.3 0 231 248 352 847.3 0 352 249 384 847.3 0 384 250 280 847.3 0 280 251 173 847.3 0 173 252 202 847.3 0 202 253 204 847.3 0 204 254 251 847.3 0 251 255 217 847.3 0 217 256 259 847.3 0 259 257 356 847.3 0 356 258 386 847.3 0 386 259 511 847.3 0 511 260 438 847.3 0 438 261 417 847.3 0 417 262 385 847.3 0 385 263 532 847.3 0 532 264 1399 847.3 0 1399 265 2981 847.3 0 2981 266 4646 847.3 0 4646 267 4106 847.3 0 4106 268 5216 847.3 0 5216 269 534 847.3 0 534 270 319 847.3 0 319 271 284 847.3 0 284 272 325 847.3 0 325 273 438 847.3 0 438 274 450 847.3 0 450 275 529 847.3 0 529 276 645 847.3 0 645 277 344 847.3 0 344 278 305 847.3 0 305 279 431 847.3 0 431 280 444 847.3 0 444 281 368 847.3 0 368 282 252 847.3 0 252 283 250 847.3 0 250 284 272 847.3 0 272 285 267 847.3 0 267 286 343 847.3 0 343 287 425 847.3 0 425 288 316 847.3 0 316 289 260 847.3 0 260 290 381 847.3 0 381 291 2338 847.3 0 2338 292 1917 847.3 0 1917 293 1396 847.3 0 1396 294 251 847.3 0 251 295 201 847.3 0 201 296 165 847.3 0 165 297 140 847.3 0 140 298 160 847.3 0 160 299 198 847.3 0 198 300 275 847.3 0 275 301 587 847.3 0 587 Finished in 15.57 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 211,085 Reads with adapters: 94,784 (44.9%) Reads that were too short: 149 (0.1%) Reads written (passing filters): 94,635 (44.8%) Total basepairs processed: 58,160,398 bp Total written (filtered): 26,199,277 bp (45.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 94784 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 9 0.0 2 0 0 0 9 15 39 0.0 3 0 0 26 13 16 778 0.0 3 0 671 69 38 17 93155 0.0 3 88589 3881 566 119 18 227 0.0 3 20 189 13 5 19 154 0.0 3 2 1 89 62 20 181 0.0 3 0 0 1 180 22 3 0.0 3 0 0 1 2 23 6 0.0 3 0 0 0 6 25 2 0.0 3 0 0 0 2 26 2 0.0 3 1 1 27 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 32 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 1 0.0 3 1 41 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 48 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 62 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 67 13 0.0 3 0 0 0 13 69 2 0.0 3 0 0 0 2 70 3 0.0 3 0 0 0 3 71 3 0.0 3 0 0 1 2 75 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 3 0.0 3 0 0 0 3 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 5 0.0 3 0 0 0 5 94 2 0.0 3 0 0 0 2 95 2 0.0 3 0 0 0 2 97 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 131 2 0.0 3 0 0 0 2 132 2 0.0 3 0 0 0 2 133 2 0.0 3 0 0 0 2 135 2 0.0 3 0 0 0 2 136 2 0.0 3 0 0 1 1 138 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 141 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 1 154 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 3 0.0 3 0 0 0 3 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 7 0.0 3 0 0 0 7 165 4 0.0 3 0 0 0 4 166 1 0.0 3 0 0 1 168 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 173 3 0.0 3 0 0 1 2 176 1 0.0 3 1 181 3 0.0 3 0 0 0 3 182 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 191 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 205 4 0.0 3 0 0 0 4 206 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 1 216 1 0.0 3 0 0 0 1 217 25 0.0 3 0 0 2 23 218 2 0.0 3 0 0 1 1 221 2 0.0 3 0 0 0 2 222 3 0.0 3 0 0 0 3 223 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 278 4 0.0 3 0 0 0 4 279 6 0.0 3 0 0 0 6 280 1 0.0 3 0 0 0 1 281 8 0.0 3 0 0 0 8 282 3 0.0 3 0 0 0 3 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.59 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 94,635 Reads with adapters: 655 (0.7%) Reads written (passing filters): 94,635 (100.0%) Total basepairs processed: 26,199,277 bp Total written (filtered): 26,197,236 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 655 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 12.8% C: 43.5% G: 29.3% T: 14.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 594 1478.7 0 594 4 49 369.7 0 49 5 9 92.4 0 9 6 3 23.1 0 3