This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 169.55 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 275,364 (7.9%) Reads that were too short: 60,891 (1.8%) Reads written (passing filters): 214,473 (6.2%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 59,445,977 bp (5.7%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 275364 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1426 847.3 0 1426 7 85656 847.3 0 85656 8 97337 847.3 0 97337 9 153 847.3 0 153 10 84 847.3 0 84 11 81 847.3 0 81 12 63 847.3 0 63 13 75 847.3 0 75 14 76 847.3 0 76 15 66 847.3 0 66 16 79 847.3 0 79 17 90 847.3 0 90 18 61 847.3 0 61 19 67 847.3 0 67 20 85 847.3 0 85 21 64 847.3 0 64 22 57 847.3 0 57 23 79 847.3 0 79 24 74 847.3 0 74 25 107 847.3 0 107 26 90 847.3 0 90 27 58 847.3 0 58 28 62 847.3 0 62 29 60 847.3 0 60 30 71 847.3 0 71 31 89 847.3 0 89 32 99 847.3 0 99 33 48 847.3 0 48 34 87 847.3 0 87 35 87 847.3 0 87 36 71 847.3 0 71 37 75 847.3 0 75 38 95 847.3 0 95 39 70 847.3 0 70 40 71 847.3 0 71 41 81 847.3 0 81 42 61 847.3 0 61 43 88 847.3 0 88 44 77 847.3 0 77 45 78 847.3 0 78 46 68 847.3 0 68 47 73 847.3 0 73 48 83 847.3 0 83 49 58 847.3 0 58 50 77 847.3 0 77 51 76 847.3 0 76 52 109 847.3 0 109 53 102 847.3 0 102 54 72 847.3 0 72 55 65 847.3 0 65 56 77 847.3 0 77 57 84 847.3 0 84 58 79 847.3 0 79 59 126 847.3 0 126 60 255 847.3 0 255 61 178 847.3 0 178 62 75 847.3 0 75 63 97 847.3 0 97 64 88 847.3 0 88 65 111 847.3 0 111 66 121 847.3 0 121 67 80 847.3 0 80 68 74 847.3 0 74 69 94 847.3 0 94 70 103 847.3 0 103 71 95 847.3 0 95 72 148 847.3 0 148 73 216 847.3 0 216 74 274 847.3 0 274 75 144 847.3 0 144 76 101 847.3 0 101 77 105 847.3 0 105 78 177 847.3 0 177 79 210 847.3 0 210 80 213 847.3 0 213 81 205 847.3 0 205 82 225 847.3 0 225 83 172 847.3 0 172 84 216 847.3 0 216 85 212 847.3 0 212 86 301 847.3 0 301 87 188 847.3 0 188 88 151 847.3 0 151 89 118 847.3 0 118 90 121 847.3 0 121 91 113 847.3 0 113 92 101 847.3 0 101 93 121 847.3 0 121 94 103 847.3 0 103 95 120 847.3 0 120 96 120 847.3 0 120 97 83 847.3 0 83 98 97 847.3 0 97 99 129 847.3 0 129 100 105 847.3 0 105 101 107 847.3 0 107 102 134 847.3 0 134 103 179 847.3 0 179 104 190 847.3 0 190 105 149 847.3 0 149 106 149 847.3 0 149 107 173 847.3 0 173 108 128 847.3 0 128 109 152 847.3 0 152 110 145 847.3 0 145 111 151 847.3 0 151 112 134 847.3 0 134 113 162 847.3 0 162 114 125 847.3 0 125 115 160 847.3 0 160 116 191 847.3 0 191 117 158 847.3 0 158 118 133 847.3 0 133 119 159 847.3 0 159 120 144 847.3 0 144 121 159 847.3 0 159 122 172 847.3 0 172 123 164 847.3 0 164 124 283 847.3 0 283 125 254 847.3 0 254 126 244 847.3 0 244 127 170 847.3 0 170 128 149 847.3 0 149 129 151 847.3 0 151 130 165 847.3 0 165 131 186 847.3 0 186 132 179 847.3 0 179 133 205 847.3 0 205 134 239 847.3 0 239 135 207 847.3 0 207 136 145 847.3 0 145 137 175 847.3 0 175 138 157 847.3 0 157 139 134 847.3 0 134 140 132 847.3 0 132 141 173 847.3 0 173 142 133 847.3 0 133 143 195 847.3 0 195 144 162 847.3 0 162 145 168 847.3 0 168 146 174 847.3 0 174 147 240 847.3 0 240 148 212 847.3 0 212 149 334 847.3 0 334 150 207 847.3 0 207 151 184 847.3 0 184 152 161 847.3 0 161 153 157 847.3 0 157 154 167 847.3 0 167 155 169 847.3 0 169 156 195 847.3 0 195 157 187 847.3 0 187 158 310 847.3 0 310 159 228 847.3 0 228 160 250 847.3 0 250 161 190 847.3 0 190 162 201 847.3 0 201 163 167 847.3 0 167 164 176 847.3 0 176 165 181 847.3 0 181 166 199 847.3 0 199 167 223 847.3 0 223 168 178 847.3 0 178 169 336 847.3 0 336 170 383 847.3 0 383 171 480 847.3 0 480 172 403 847.3 0 403 173 203 847.3 0 203 174 199 847.3 0 199 175 194 847.3 0 194 176 162 847.3 0 162 177 145 847.3 0 145 178 177 847.3 0 177 179 216 847.3 0 216 180 217 847.3 0 217 181 221 847.3 0 221 182 189 847.3 0 189 183 195 847.3 0 195 184 221 847.3 0 221 185 233 847.3 0 233 186 173 847.3 0 173 187 341 847.3 0 341 188 356 847.3 0 356 189 277 847.3 0 277 190 225 847.3 0 225 191 187 847.3 0 187 192 230 847.3 0 230 193 217 847.3 0 217 194 218 847.3 0 218 195 214 847.3 0 214 196 188 847.3 0 188 197 161 847.3 0 161 198 175 847.3 0 175 199 233 847.3 0 233 200 266 847.3 0 266 201 217 847.3 0 217 202 193 847.3 0 193 203 280 847.3 0 280 204 310 847.3 0 310 205 331 847.3 0 331 206 168 847.3 0 168 207 161 847.3 0 161 208 140 847.3 0 140 209 168 847.3 0 168 210 157 847.3 0 157 211 194 847.3 0 194 212 185 847.3 0 185 213 219 847.3 0 219 214 574 847.3 0 574 215 1012 847.3 0 1012 216 1351 847.3 0 1351 217 1402 847.3 0 1402 218 275 847.3 0 275 219 297 847.3 0 297 220 213 847.3 0 213 221 192 847.3 0 192 222 226 847.3 0 226 223 259 847.3 0 259 224 353 847.3 0 353 225 541 847.3 0 541 226 1923 847.3 0 1923 227 3872 847.3 0 3872 228 4840 847.3 0 4840 229 8604 847.3 0 8604 230 1305 847.3 0 1305 231 1999 847.3 0 1999 232 1763 847.3 0 1763 233 3308 847.3 0 3308 234 196 847.3 0 196 235 136 847.3 0 136 236 156 847.3 0 156 237 179 847.3 0 179 238 208 847.3 0 208 239 257 847.3 0 257 240 203 847.3 0 203 241 168 847.3 0 168 242 146 847.3 0 146 243 156 847.3 0 156 244 172 847.3 0 172 245 176 847.3 0 176 246 134 847.3 0 134 247 218 847.3 0 218 248 294 847.3 0 294 249 313 847.3 0 313 250 282 847.3 0 282 251 180 847.3 0 180 252 164 847.3 0 164 253 152 847.3 0 152 254 213 847.3 0 213 255 229 847.3 0 229 256 196 847.3 0 196 257 218 847.3 0 218 258 247 847.3 0 247 259 263 847.3 0 263 260 287 847.3 0 287 261 275 847.3 0 275 262 237 847.3 0 237 263 272 847.3 0 272 264 672 847.3 0 672 265 1268 847.3 0 1268 266 2113 847.3 0 2113 267 1791 847.3 0 1791 268 2228 847.3 0 2228 269 355 847.3 0 355 270 215 847.3 0 215 271 220 847.3 0 220 272 218 847.3 0 218 273 317 847.3 0 317 274 362 847.3 0 362 275 361 847.3 0 361 276 422 847.3 0 422 277 271 847.3 0 271 278 217 847.3 0 217 279 268 847.3 0 268 280 299 847.3 0 299 281 247 847.3 0 247 282 216 847.3 0 216 283 197 847.3 0 197 284 210 847.3 0 210 285 218 847.3 0 218 286 287 847.3 0 287 287 261 847.3 0 261 288 219 847.3 0 219 289 189 847.3 0 189 290 198 847.3 0 198 291 1026 847.3 0 1026 292 803 847.3 0 803 293 644 847.3 0 644 294 219 847.3 0 219 295 153 847.3 0 153 296 140 847.3 0 140 297 113 847.3 0 113 298 153 847.3 0 153 299 172 847.3 0 172 300 219 847.3 0 219 301 268 847.3 0 268 Finished in 16.99 s (79 us/read; 0.76 M reads/minute). === Summary === Total reads processed: 214,473 Reads with adapters: 100,024 (46.6%) Reads that were too short: 31 (0.0%) Reads written (passing filters): 99,993 (46.6%) Total basepairs processed: 59,445,977 bp Total written (filtered): 27,198,494 bp (45.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 100024 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 45 0.0 3 0 0 44 1 19 1251 0.0 3 0 1225 19 5 2 20 97408 0.0 4 95665 1673 64 5 1 21 234 0.0 4 30 198 6 22 17 0.0 4 1 0 16 23 65 0.0 4 0 0 0 55 10 24 2 0.0 4 1 0 0 0 1 26 2 0.0 4 0 0 0 1 1 30 1 0.0 4 0 0 1 33 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 1 119 1 0.0 4 0 0 0 0 1 120 4 0.0 4 0 2 1 0 1 121 1 0.0 4 0 1 124 2 0.0 4 0 0 0 0 2 125 1 0.0 4 0 0 0 0 1 126 9 0.0 4 0 0 0 0 9 127 941 0.0 4 0 0 0 0 941 128 4 0.0 4 0 0 0 0 4 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 3 0.0 4 0 0 0 0 3 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 1 250 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 269 1 0.0 4 0 0 0 0 1 288 3 0.0 4 0 0 0 0 3 293 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.63 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 99,993 Reads with adapters: 6,809 (6.8%) Reads written (passing filters): 99,993 (100.0%) Total basepairs processed: 27,198,494 bp Total written (filtered): 27,124,022 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6809 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 2.4% G: 94.8% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 40 1562.4 0 40 4 26 390.6 0 26 5 8 97.6 0 8 6 3 24.4 0 3 8 1 1.5 0 1 9 93 0.4 0 3 90 10 1759 0.1 1 57 1702 11 4838 0.0 1 27 4811 12 15 0.0 1 2 13 13 2 0.0 1 0 2 14 4 0.0 1 0 4 19 8 0.0 1 7 1 20 1 0.0 1 1 148 1 0.0 1 1 151 2 0.0 1 0 2 190 1 0.0 1 0 1 209 7 0.0 1 0 7 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.