This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 163.74 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 71,081 (2.0%) Reads that were too short: 12,037 (0.3%) Reads written (passing filters): 59,044 (1.7%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 13,060,958 bp (1.3%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 71081 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 580 847.3 0 580 7 10311 847.3 0 10311 8 255 847.3 0 255 9 10714 847.3 0 10714 10 12 847.3 0 12 11 7 847.3 0 7 12 2 847.3 0 2 13 2 847.3 0 2 14 1 847.3 0 1 15 3 847.3 0 3 16 2 847.3 0 2 17 1 847.3 0 1 19 5 847.3 0 5 20 5 847.3 0 5 21 2 847.3 0 2 22 15 847.3 0 15 23 18 847.3 0 18 24 3 847.3 0 3 25 45 847.3 0 45 26 19 847.3 0 19 27 10 847.3 0 10 28 6 847.3 0 6 29 6 847.3 0 6 30 10 847.3 0 10 31 3 847.3 0 3 32 13 847.3 0 13 33 27 847.3 0 27 34 630 847.3 0 630 35 388 847.3 0 388 36 11 847.3 0 11 37 6 847.3 0 6 38 4 847.3 0 4 39 12 847.3 0 12 40 14 847.3 0 14 41 11 847.3 0 11 42 3 847.3 0 3 43 4 847.3 0 4 44 5 847.3 0 5 45 2 847.3 0 2 46 3 847.3 0 3 47 2 847.3 0 2 48 2 847.3 0 2 49 1 847.3 0 1 50 5 847.3 0 5 51 2 847.3 0 2 52 8 847.3 0 8 53 9 847.3 0 9 54 5 847.3 0 5 55 3 847.3 0 3 56 6 847.3 0 6 57 4 847.3 0 4 58 6 847.3 0 6 59 7 847.3 0 7 60 9 847.3 0 9 61 5 847.3 0 5 62 3 847.3 0 3 63 5 847.3 0 5 64 4 847.3 0 4 65 4 847.3 0 4 66 8 847.3 0 8 67 8 847.3 0 8 68 8 847.3 0 8 69 3 847.3 0 3 70 9 847.3 0 9 71 4 847.3 0 4 72 8 847.3 0 8 73 10 847.3 0 10 74 115 847.3 0 115 75 41 847.3 0 41 76 42 847.3 0 42 77 50 847.3 0 50 78 50 847.3 0 50 79 31 847.3 0 31 80 19 847.3 0 19 81 36 847.3 0 36 82 39 847.3 0 39 83 31 847.3 0 31 84 18 847.3 0 18 85 24 847.3 0 24 86 41 847.3 0 41 87 45 847.3 0 45 88 44 847.3 0 44 89 59 847.3 0 59 90 49 847.3 0 49 91 41 847.3 0 41 92 35 847.3 0 35 93 161 847.3 0 161 94 95 847.3 0 95 95 89 847.3 0 89 96 85 847.3 0 85 97 202 847.3 0 202 98 234 847.3 0 234 99 1328 847.3 0 1328 100 798 847.3 0 798 101 554 847.3 0 554 102 75 847.3 0 75 103 65 847.3 0 65 104 77 847.3 0 77 105 57 847.3 0 57 106 72 847.3 0 72 107 81 847.3 0 81 108 79 847.3 0 79 109 152 847.3 0 152 110 276 847.3 0 276 111 3116 847.3 0 3116 112 3316 847.3 0 3316 113 3985 847.3 0 3985 114 2421 847.3 0 2421 115 1704 847.3 0 1704 116 40 847.3 0 40 117 32 847.3 0 32 118 41 847.3 0 41 119 66 847.3 0 66 120 30 847.3 0 30 121 41 847.3 0 41 122 33 847.3 0 33 123 32 847.3 0 32 124 20 847.3 0 20 125 63 847.3 0 63 126 45 847.3 0 45 127 88 847.3 0 88 128 91 847.3 0 91 129 865 847.3 0 865 130 320 847.3 0 320 131 225 847.3 0 225 132 104 847.3 0 104 133 99 847.3 0 99 134 69 847.3 0 69 135 181 847.3 0 181 136 189 847.3 0 189 137 166 847.3 0 166 138 111 847.3 0 111 139 106 847.3 0 106 140 126 847.3 0 126 141 182 847.3 0 182 142 1522 847.3 0 1522 143 4230 847.3 0 4230 144 2732 847.3 0 2732 145 1145 847.3 0 1145 146 78 847.3 0 78 147 65 847.3 0 65 148 49 847.3 0 49 149 46 847.3 0 46 150 29 847.3 0 29 151 31 847.3 0 31 152 43 847.3 0 43 153 39 847.3 0 39 154 38 847.3 0 38 155 42 847.3 0 42 156 49 847.3 0 49 157 59 847.3 0 59 158 88 847.3 0 88 159 49 847.3 0 49 160 62 847.3 0 62 161 57 847.3 0 57 162 77 847.3 0 77 163 34 847.3 0 34 164 33 847.3 0 33 165 46 847.3 0 46 166 60 847.3 0 60 167 30 847.3 0 30 168 41 847.3 0 41 169 40 847.3 0 40 170 44 847.3 0 44 171 36 847.3 0 36 172 33 847.3 0 33 173 30 847.3 0 30 174 38 847.3 0 38 175 33 847.3 0 33 176 44 847.3 0 44 177 67 847.3 0 67 178 65 847.3 0 65 179 53 847.3 0 53 180 53 847.3 0 53 181 51 847.3 0 51 182 55 847.3 0 55 183 50 847.3 0 50 184 41 847.3 0 41 185 48 847.3 0 48 186 44 847.3 0 44 187 70 847.3 0 70 188 85 847.3 0 85 189 80 847.3 0 80 190 107 847.3 0 107 191 52 847.3 0 52 192 44 847.3 0 44 193 45 847.3 0 45 194 45 847.3 0 45 195 73 847.3 0 73 196 55 847.3 0 55 197 70 847.3 0 70 198 48 847.3 0 48 199 45 847.3 0 45 200 55 847.3 0 55 201 56 847.3 0 56 202 64 847.3 0 64 203 81 847.3 0 81 204 63 847.3 0 63 205 43 847.3 0 43 206 49 847.3 0 49 207 66 847.3 0 66 208 67 847.3 0 67 209 58 847.3 0 58 210 65 847.3 0 65 211 60 847.3 0 60 212 42 847.3 0 42 213 59 847.3 0 59 214 57 847.3 0 57 215 49 847.3 0 49 216 56 847.3 0 56 217 58 847.3 0 58 218 55 847.3 0 55 219 47 847.3 0 47 220 75 847.3 0 75 221 90 847.3 0 90 222 91 847.3 0 91 223 116 847.3 0 116 224 157 847.3 0 157 225 77 847.3 0 77 226 356 847.3 0 356 227 36 847.3 0 36 228 33 847.3 0 33 229 72 847.3 0 72 230 60 847.3 0 60 231 53 847.3 0 53 232 63 847.3 0 63 233 83 847.3 0 83 234 91 847.3 0 91 235 95 847.3 0 95 236 86 847.3 0 86 237 75 847.3 0 75 238 102 847.3 0 102 239 89 847.3 0 89 240 103 847.3 0 103 241 143 847.3 0 143 242 127 847.3 0 127 243 149 847.3 0 149 244 102 847.3 0 102 245 82 847.3 0 82 246 70 847.3 0 70 247 61 847.3 0 61 248 76 847.3 0 76 249 60 847.3 0 60 250 80 847.3 0 80 251 79 847.3 0 79 252 117 847.3 0 117 253 96 847.3 0 96 254 100 847.3 0 100 255 97 847.3 0 97 256 122 847.3 0 122 257 198 847.3 0 198 258 177 847.3 0 177 259 138 847.3 0 138 260 93 847.3 0 93 261 92 847.3 0 92 262 174 847.3 0 174 263 209 847.3 0 209 264 232 847.3 0 232 265 86 847.3 0 86 266 61 847.3 0 61 267 53 847.3 0 53 268 63 847.3 0 63 269 65 847.3 0 65 270 103 847.3 0 103 271 116 847.3 0 116 272 93 847.3 0 93 273 78 847.3 0 78 274 109 847.3 0 109 275 198 847.3 0 198 276 146 847.3 0 146 277 182 847.3 0 182 278 125 847.3 0 125 279 80 847.3 0 80 280 103 847.3 0 103 281 104 847.3 0 104 282 153 847.3 0 153 283 241 847.3 0 241 284 358 847.3 0 358 285 201 847.3 0 201 286 90 847.3 0 90 287 84 847.3 0 84 288 66 847.3 0 66 289 99 847.3 0 99 290 541 847.3 0 541 291 788 847.3 0 788 292 277 847.3 0 277 293 192 847.3 0 192 294 120 847.3 0 120 295 390 847.3 0 390 296 142 847.3 0 142 297 103 847.3 0 103 298 150 847.3 0 150 299 133 847.3 0 133 300 128 847.3 0 128 301 100 847.3 0 100 Finished in 3.82 s (65 us/read; 0.93 M reads/minute). === Summary === Total reads processed: 59,044 Reads with adapters: 11,457 (19.4%) Reads that were too short: 108 (0.2%) Reads written (passing filters): 11,349 (19.2%) Total basepairs processed: 13,060,958 bp Total written (filtered): 3,085,222 bp (23.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 11457 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 9 0.0 3 0 0 6 3 16 212 0.0 3 0 194 15 3 17 10789 0.0 3 10195 495 84 15 18 40 0.0 3 1 24 6 9 19 23 0.0 3 1 5 8 9 20 21 0.0 3 0 0 0 21 21 1 0.0 3 0 0 0 1 22 1 0.0 3 0 0 0 1 23 15 0.0 3 0 0 0 15 24 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 95 0.0 3 0 0 1 94 30 1 0.0 3 0 0 0 1 32 18 0.0 3 0 0 0 18 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 55 3 0.0 3 0 0 0 3 56 6 0.0 3 0 0 0 6 57 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 12 0.0 3 0 0 0 12 64 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 67 2 0.0 3 0 0 0 2 72 23 0.0 3 0 0 0 23 73 16 0.0 3 0 0 0 16 74 11 0.0 3 0 0 0 11 75 4 0.0 3 0 0 1 3 76 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 78 3 0.0 3 0 0 1 2 80 3 0.0 3 0 0 0 3 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 92 5 0.0 3 0 0 0 5 93 3 0.0 3 0 0 0 3 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 138 4 0.0 3 0 0 0 4 140 1 0.0 3 0 0 0 1 141 17 0.0 3 0 0 1 16 142 8 0.0 3 0 0 0 8 143 1 0.0 3 0 0 1 144 23 0.0 3 0 0 0 23 145 5 0.0 3 0 0 0 5 146 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 2 0.0 3 0 0 0 2 186 4 0.0 3 0 0 0 4 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 1 189 1 0.0 3 0 0 1 196 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 280 3 0.0 3 0 0 0 3 281 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.66 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 11,349 Reads with adapters: 790 (7.0%) Reads written (passing filters): 11,349 (100.0%) Total basepairs processed: 3,085,222 bp Total written (filtered): 3,082,839 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 790 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 79.2% C: 12.4% G: 7.6% T: 0.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 778 177.3 0 778 4 11 44.3 0 11 5 1 11.1 0 1