This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 163.26 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 66,186 (1.9%) Reads that were too short: 9,760 (0.3%) Reads written (passing filters): 56,426 (1.6%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 12,583,298 bp (1.2%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 66186 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 581 847.3 0 581 7 12671 847.3 0 12671 8 182 847.3 0 182 9 9602 847.3 0 9602 10 4 847.3 0 4 11 2 847.3 0 2 12 4 847.3 0 4 13 2 847.3 0 2 14 1 847.3 0 1 15 2 847.3 0 2 16 1 847.3 0 1 17 1 847.3 0 1 18 1 847.3 0 1 19 1 847.3 0 1 21 4 847.3 0 4 23 2 847.3 0 2 24 2 847.3 0 2 25 1 847.3 0 1 26 3 847.3 0 3 27 1 847.3 0 1 28 4 847.3 0 4 29 2 847.3 0 2 30 1 847.3 0 1 32 4 847.3 0 4 33 3 847.3 0 3 34 3 847.3 0 3 35 7 847.3 0 7 36 1 847.3 0 1 37 3 847.3 0 3 38 3 847.3 0 3 39 5 847.3 0 5 40 32 847.3 0 32 41 10 847.3 0 10 42 1 847.3 0 1 44 4 847.3 0 4 45 5 847.3 0 5 46 5 847.3 0 5 47 3 847.3 0 3 48 10 847.3 0 10 49 3 847.3 0 3 50 4 847.3 0 4 51 1 847.3 0 1 52 4 847.3 0 4 53 5 847.3 0 5 54 1 847.3 0 1 55 3 847.3 0 3 56 3 847.3 0 3 57 2 847.3 0 2 58 4 847.3 0 4 59 5 847.3 0 5 60 5 847.3 0 5 61 4 847.3 0 4 62 3 847.3 0 3 64 1 847.3 0 1 66 18 847.3 0 18 67 2 847.3 0 2 68 4 847.3 0 4 69 6 847.3 0 6 70 5 847.3 0 5 71 6 847.3 0 6 72 1 847.3 0 1 73 3 847.3 0 3 74 4 847.3 0 4 75 2 847.3 0 2 76 5 847.3 0 5 77 7 847.3 0 7 78 14 847.3 0 14 79 21 847.3 0 21 80 14 847.3 0 14 81 23 847.3 0 23 82 15 847.3 0 15 83 28 847.3 0 28 84 29 847.3 0 29 85 19 847.3 0 19 86 41 847.3 0 41 87 23 847.3 0 23 88 36 847.3 0 36 89 48 847.3 0 48 90 28 847.3 0 28 91 42 847.3 0 42 92 49 847.3 0 49 93 52 847.3 0 52 94 58 847.3 0 58 95 58 847.3 0 58 96 67 847.3 0 67 97 121 847.3 0 121 98 183 847.3 0 183 99 641 847.3 0 641 100 426 847.3 0 426 101 279 847.3 0 279 102 78 847.3 0 78 103 89 847.3 0 89 104 83 847.3 0 83 105 51 847.3 0 51 106 77 847.3 0 77 107 70 847.3 0 70 108 90 847.3 0 90 109 139 847.3 0 139 110 227 847.3 0 227 111 3133 847.3 0 3133 112 3298 847.3 0 3298 113 3952 847.3 0 3952 114 2352 847.3 0 2352 115 1547 847.3 0 1547 116 33 847.3 0 33 117 30 847.3 0 30 118 37 847.3 0 37 119 33 847.3 0 33 120 34 847.3 0 34 121 25 847.3 0 25 122 34 847.3 0 34 123 32 847.3 0 32 124 25 847.3 0 25 125 44 847.3 0 44 126 36 847.3 0 36 127 85 847.3 0 85 128 85 847.3 0 85 129 650 847.3 0 650 130 210 847.3 0 210 131 177 847.3 0 177 132 76 847.3 0 76 133 89 847.3 0 89 134 73 847.3 0 73 135 164 847.3 0 164 136 172 847.3 0 172 137 141 847.3 0 141 138 89 847.3 0 89 139 90 847.3 0 90 140 132 847.3 0 132 141 159 847.3 0 159 142 1730 847.3 0 1730 143 4702 847.3 0 4702 144 3060 847.3 0 3060 145 1162 847.3 0 1162 146 62 847.3 0 62 147 49 847.3 0 49 148 35 847.3 0 35 149 28 847.3 0 28 150 24 847.3 0 24 151 33 847.3 0 33 152 35 847.3 0 35 153 30 847.3 0 30 154 28 847.3 0 28 155 21 847.3 0 21 156 50 847.3 0 50 157 57 847.3 0 57 158 62 847.3 0 62 159 45 847.3 0 45 160 48 847.3 0 48 161 44 847.3 0 44 162 38 847.3 0 38 163 28 847.3 0 28 164 34 847.3 0 34 165 27 847.3 0 27 166 55 847.3 0 55 167 22 847.3 0 22 168 30 847.3 0 30 169 30 847.3 0 30 170 26 847.3 0 26 171 26 847.3 0 26 172 25 847.3 0 25 173 25 847.3 0 25 174 34 847.3 0 34 175 31 847.3 0 31 176 21 847.3 0 21 177 55 847.3 0 55 178 47 847.3 0 47 179 33 847.3 0 33 180 37 847.3 0 37 181 28 847.3 0 28 182 33 847.3 0 33 183 34 847.3 0 34 184 25 847.3 0 25 185 37 847.3 0 37 186 27 847.3 0 27 187 40 847.3 0 40 188 64 847.3 0 64 189 63 847.3 0 63 190 126 847.3 0 126 191 40 847.3 0 40 192 31 847.3 0 31 193 53 847.3 0 53 194 40 847.3 0 40 195 61 847.3 0 61 196 43 847.3 0 43 197 56 847.3 0 56 198 41 847.3 0 41 199 36 847.3 0 36 200 25 847.3 0 25 201 53 847.3 0 53 202 46 847.3 0 46 203 42 847.3 0 42 204 47 847.3 0 47 205 34 847.3 0 34 206 55 847.3 0 55 207 56 847.3 0 56 208 80 847.3 0 80 209 51 847.3 0 51 210 57 847.3 0 57 211 38 847.3 0 38 212 41 847.3 0 41 213 41 847.3 0 41 214 38 847.3 0 38 215 43 847.3 0 43 216 56 847.3 0 56 217 47 847.3 0 47 218 46 847.3 0 46 219 47 847.3 0 47 220 58 847.3 0 58 221 51 847.3 0 51 222 54 847.3 0 54 223 79 847.3 0 79 224 148 847.3 0 148 225 91 847.3 0 91 226 398 847.3 0 398 227 28 847.3 0 28 228 37 847.3 0 37 229 57 847.3 0 57 230 47 847.3 0 47 231 46 847.3 0 46 232 44 847.3 0 44 233 53 847.3 0 53 234 86 847.3 0 86 235 63 847.3 0 63 236 44 847.3 0 44 237 65 847.3 0 65 238 67 847.3 0 67 239 86 847.3 0 86 240 78 847.3 0 78 241 104 847.3 0 104 242 102 847.3 0 102 243 112 847.3 0 112 244 80 847.3 0 80 245 67 847.3 0 67 246 57 847.3 0 57 247 47 847.3 0 47 248 53 847.3 0 53 249 59 847.3 0 59 250 61 847.3 0 61 251 69 847.3 0 69 252 97 847.3 0 97 253 73 847.3 0 73 254 63 847.3 0 63 255 92 847.3 0 92 256 103 847.3 0 103 257 143 847.3 0 143 258 131 847.3 0 131 259 72 847.3 0 72 260 60 847.3 0 60 261 67 847.3 0 67 262 95 847.3 0 95 263 123 847.3 0 123 264 112 847.3 0 112 265 51 847.3 0 51 266 35 847.3 0 35 267 51 847.3 0 51 268 68 847.3 0 68 269 59 847.3 0 59 270 83 847.3 0 83 271 66 847.3 0 66 272 55 847.3 0 55 273 74 847.3 0 74 274 62 847.3 0 62 275 164 847.3 0 164 276 83 847.3 0 83 277 124 847.3 0 124 278 66 847.3 0 66 279 66 847.3 0 66 280 67 847.3 0 67 281 89 847.3 0 89 282 124 847.3 0 124 283 191 847.3 0 191 284 284 847.3 0 284 285 119 847.3 0 119 286 61 847.3 0 61 287 72 847.3 0 72 288 78 847.3 0 78 289 96 847.3 0 96 290 511 847.3 0 511 291 813 847.3 0 813 292 251 847.3 0 251 293 174 847.3 0 174 294 122 847.3 0 122 295 372 847.3 0 372 296 148 847.3 0 148 297 100 847.3 0 100 298 107 847.3 0 107 299 97 847.3 0 97 300 105 847.3 0 105 301 85 847.3 0 85 Finished in 3.94 s (70 us/read; 0.86 M reads/minute). === Summary === Total reads processed: 56,426 Reads with adapters: 13,266 (23.5%) Reads that were too short: 27 (0.0%) Reads written (passing filters): 13,239 (23.5%) Total basepairs processed: 12,583,298 bp Total written (filtered): 3,611,158 bp (28.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 13266 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 6 0.0 3 0 0 6 19 121 0.0 3 0 119 2 20 12877 0.0 4 12631 240 5 1 21 53 0.0 4 36 17 22 13 0.0 4 10 0 3 23 12 0.0 4 2 0 0 10 25 1 0.0 4 1 27 1 0.0 4 1 30 1 0.0 4 0 0 0 0 1 35 6 0.0 4 0 0 0 0 6 73 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 122 10 0.0 4 0 0 0 0 10 123 2 0.0 4 0 0 0 0 2 126 1 0.0 4 0 0 0 0 1 127 146 0.0 4 0 0 0 0 146 135 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 142 2 0.0 4 0 0 0 0 2 162 1 0.0 4 1 189 1 0.0 4 0 0 0 0 1 217 1 0.0 4 1 228 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.75 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 13,239 Reads with adapters: 231 (1.7%) Reads written (passing filters): 13,239 (100.0%) Total basepairs processed: 3,611,158 bp Total written (filtered): 3,608,292 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 231 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 13.0% C: 6.9% G: 78.4% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 24 206.9 0 24 4 4 51.7 0 4 5 2 12.9 0 2 10 1 0.0 1 0 1 11 176 0.0 1 0 176 12 10 0.0 1 1 9 13 1 0.0 1 0 1 14 1 0.0 1 0 1 15 1 0.0 1 0 1 20 7 0.0 1 5 2 24 1 0.0 1 1 61 1 0.0 1 1 210 1 0.0 1 1 225 1 0.0 1 1